On 28 January 2020, three living individuals of the Leisler’s bat, Nyctalus leisleri, were found in a shed in Malaucène, southern France (44°11’34”N, 05°07’26”E). Two of these bats had bands with the inscription CESON.ORG used in the Czech Republic since 2019. The whole ring code was detected in one case (CZ03313), in a female captured and banded in a bat-box near Bečov nad Teplou, western Bohemia, Czech Republic (50°05’27”N, 12°51’38”E) on 9 September 2019, 878 km away from Malaucène. This is the first case of a long distance migration in the population of Nyctalus leisleri of the Czech Republic. This observation also gives a direct evidence of a flight during the maximum 141 days in late summer or autumn from the central or northeastern Europe to the southwestern parts of the continent.