scholarly journals PL-4 (CIP596131.4): an Improved Potato Haploid Inducer

2021 ◽  
Author(s):  
Benny Ordoñez ◽  
Monica Santayana ◽  
Mariela Aponte ◽  
Isabelle M. Henry ◽  
Luca Comai ◽  
...  
Keyword(s):  
Genetic Background ◽  
Potato Breeding ◽  
Haploid Induction ◽  
Breeding Lines ◽  
International Potato ◽  
Haploid Induction Rate ◽  
Induction Rate ◽  
Hybrid Seeds ◽  
Efficient Screening ◽  
Potato Genetics

AbstractDihaploid production from elite tetraploid cultivars is key to both traditional and novel breeding approaches that seek to simplify potato genetics. For this purpose, efficient and widely compatible haploid inducers (HIs) are needed. We compared PL-4, a new HI developed at the International Potato Center, to known HIs IvP101 and IvP35. By pollination of elite tetraploid breeding lines, we showed that PL-4 performed significantly better and had a homogeneous response regardless of the genetic background of the pistillate parents, on the most important efficiency traits—number of dihaploids per 100 fruits and haploid induction rate. Moreover, PL-4 exhibited a reduced proportion of hybrid seeds, a convenient trait for efficient screening. In this context, we recommend PL-4 as an enhanced HI for the potato breeding community.

scholarly journals MYO, a Candidate Gene for Haploid Induction in Maize Causes Male Sterility

Plants ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 773
Author(s):  
Kimberly Vanous ◽  
Thomas Lübberstedt ◽  
Rania Ibrahim ◽  
Ursula K. Frei
Keyword(s):  
Male Sterility ◽  
Gene Isolation ◽  
Haploid Induction ◽  
Maize Breeding ◽  
Breeding Programs ◽  
Positional Candidate ◽  
Haploid Induction Rate ◽  
Induction Rate ◽  
Transgenic Lines ◽  
Strong Candidate

Doubled haploid technology is highly successful in maize breeding programs and is contingent on the ability of maize inducers to efficiently produce haploids. Knowledge of the genes involved in haploid induction is important for not only developing better maize inducers, but also to create inducers in other crops. The main quantitative trait loci involved in maize haploid induction are qhir1 and qhir8. The gene underlying qhir1 has been discovered and validated by independent research groups. Prior to initiation of this study, the gene associated with qhir8 had yet to be recognized. Therefore, this research focused on characterizing positional candidate genes underlying qhir8. Pursuing this goal, a strong candidate for qhir8, GRMZM2G435294 (MYO), was silenced by RNAi. Analysis of crosses with these heterozygous RNAi-transgenic lines for haploid induction rate revealed that the silencing of MYO significantly enhanced haploid induction rate by an average of 0.6% in the presence of qhir1. Recently, GRMZM2G465053 (ZmDMP) was identified by map-based gene isolation and shown to be responsible for qhir8. While our results suggest that MYO may contribute to haploid induction rate, results were inconsistent and only showing minor increases in haploid induction rate compared to ZmDMP. Instead, reciprocal crosses clearly revealed that the silencing of MYO causes male sterility.

scholarly journals Selection Gain of Maize Haploid Inducers for the Tropical Savanna Environments

Plants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 2812
Author(s):  
Abil Dermail ◽  
Sompong Chankaew ◽  
Kamol Lertrat ◽  
Thomas Lübberstedt ◽  
Khundej Suriharn
Keyword(s):  
Genetic Gain ◽  
Seed Set ◽  
Agronomic Traits ◽  
Phenotypic Diversity ◽  
Hybrid Breeding ◽  
Agronomic Performance ◽  
Tropical Savanna ◽  
Haploid Induction ◽  
Haploid Induction Rate ◽  
Induction Rate

Lacking elite haploid inducers performing high haploid induction rate (HIR) and agronomic performance is one of fundamental factors hindering the rapid adoption of doubled haploid technology in maize hybrid breeding, especially under tropical savanna climate. Breeding haploid inducers for specific agro-ecology, thus, is indispensable yet challenging. We used temperate inducer Stock6 as genetic source for haploid induction ability and eight tropical maize genotypes as principal donors for agronomic adaptation. Three cycles of modified ear-to-row with 5% intra-family selection were applied in a population set of 78 putative haploid inducer families emphasized on agronomic performance, R1-nj anthocyanin intensity, and inducer seed set. Genetic gains, variance components, and heritability on given traits were estimated. Hierarchical clustering based on five selection criteria was performed to investigate the phenotypic diversity of putative families. Cycle effect was predominant for all observed traits. Realized genetic gain was positive for HIR (0.40% per cycle) and inducer seed set (30.10% or 47.30 seeds per ear per cycle). In this study, we reported the first haploid inducers for regions under tropical savanna climate. Three inducer families, KHI-42, KHI-54, and KHI-64, were promising as they possessed HIR about 7.8% or 14 haploid seeds per tester ear and inducer seed rate about 95.0% or 208 inducer seeds per ear. The breeding method was effective for enhancing the seed set and the expression of R1-nj anthocyanin marker of inducers, yet it showed a low effectiveness to improve haploid induction rate. Introgression of temperate inducer Stock6 into tropical gene pool followed by phenotypic selections through modified ear-to-row selection on inducer seed set and R1-nj marker did not compromise the agronomic traits of tropical inducer families. Implications and further strategies for optimizing genetic gain on HIR are discussed.

scholarly journals Development of In Vivo Haploid Inducer Lines for Screening Haploid Immature Embryos in Maize

Plants ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 739
Author(s):  
Chen Chen ◽  
Zijian Xiao ◽  
Junwen Zhang ◽  
Wei Li ◽  
Jinlong Li ◽  
...  
Keyword(s):  
Doubled Haploid ◽  
Immature Embryos ◽  
Haploid Induction ◽  
Haploid Induction Rate ◽  
Induction Rate ◽  
Haploid Inducer ◽  
Breeding Populations ◽  
Selection For ◽  
Doubled Haploid Breeding

Doubled haploid technology is widely applied in maize. The haploid inducer lines play critical roles in doubled haploid breeding. We report the development of specialized haploid inducer lines that enhance the purple pigmentation of crossing immature embryos. During the development of haploid inducer lines, two breeding populations derived from the CAU3/S23 and CAU5/S23 were used. Molecular marker-assisted selection for both qhir1 and qhir8 was used from BC1F1 to BC1F4. Evaluation of the candidate individuals in each generation was carried out by pollinating to the tester of ZD958. Individuals with fast and clear pigmentation of the crossing immature embryos, high number of haploids per ear, and high haploid induction rate were considered as candidates. Finally, three new haploid inducer lines (CS1, CS2, and CS3) were developed. The first two (CS1 and CS2) were from the CAU3/S23, with a haploid induction rate of 8.29%–13.25% and 11.54%–15.54%, respectively. Meanwhile, the CS3 was from the CAU5/S23. Its haploid induction rate was 8.14%–12.28%. In comparison with the donor haploid inducer lines, the 24-h purple embryo rates of the newly developed haploid inducer lines were improved by 10%–20%, with a ~90% accuracy for the identification of haploid immature embryos. These new haploid inducer lines will further improve the efficiency of doubled haploid breeding of maize.

Effect of source germplasm and season on the in vivo haploid induction rate in tropical maize

Euphytica ◽  
2011 ◽  
Vol 180 (2) ◽  
pp. 219-226 ◽  
Author(s):  
Aida Z. Kebede ◽  
Baldev S. Dhillon ◽  
Wolfgang Schipprack ◽  
Jose L. Araus ◽  
Marianne Bänziger ◽  
...  
Keyword(s):  
Tropical Maize ◽  
Haploid Induction ◽  
Haploid Induction Rate ◽  
Induction Rate

Mapping of maternal QTLs for in vivo haploid induction rate in maize (Zea mays L.)

Euphytica ◽  
2013 ◽  
Vol 196 (3) ◽  
pp. 413-421 ◽  
Author(s):  
Penghao Wu ◽  
Haochuan Li ◽  
Jiaojiao Ren ◽  
Shaojiang Chen
Keyword(s):  
Zea Mays ◽  
Zea Mays L ◽  
Haploid Induction ◽  
Haploid Induction Rate ◽  
Induction Rate

scholarly journals Genomic prediction of maternal haploid induction rate in maize

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Vinícius Costa Almeida ◽  
Henrique Uliana Trentin ◽  
Ursula Karoline Frei ◽  
Thomas Lübberstedt
Keyword(s):  
Genomic Prediction ◽  
Haploid Induction ◽  
Haploid Induction Rate ◽  
Induction Rate

scholarly journals The genotypic response towards haploid induction in Turkish onion (Allium cepa L.) germplasm

2020 ◽  
Vol 48 (3) ◽  
pp. 1176-1184
Author(s):  
Faika YARALI KARAKAN
Keyword(s):  
Flow Cytometry ◽  
Allium Cepa ◽  
Ms Medium ◽  
Haploid Induction ◽  
Flower Buds ◽  
Plant Survival ◽  
Survival Percentage ◽  
Induction Rate ◽  
Allium Cepa L ◽  
Medium Flow

Haploid induction efficiency of unpollinated flower buds in twenty-six Turkish onion germplasm were determined on two media, Dunstan and Short medium (BDS) and Murashige and Skoog’s medium (MS). On BDS medium, 5850 flower buds were cultured and the induction rate was 2.10% and a plant survival percentage of 1.62% (2 plants). On MS medium, 5850 flower buds were cultured and 175 plantlets were induced; induction rate was 2.99% and plant survival percentage was 13.71% (24 plants). The highest number of plantlets was obtained from genotype Sanliurfa 2; 27 plantlets on BDS medium while 45 plantlets on MS medium. Flow cytometry confirmed that out of the twenty-six plants, 4 plants were haploid (15.38%), 2 mixoploid (7.69%), 18 (69.23%) diploid and 2 tetraploid (7.69%).

scholarly journals Carotenoid Profiles of Potato Breeding Lines and Selected Cultivars

HortScience ◽  
2003 ◽  
Vol 38 (6) ◽  
pp. 1173-1177 ◽  
Author(s):  
Sergey Nesterenko ◽  
Kenneth C. Sink
Keyword(s):  
Solanum Tuberosum ◽  
Potato Breeding ◽  
Eye Diseases ◽  
Breeding Line ◽  
Fresh Weight ◽  
Breeding Lines ◽  
Low Level ◽  
Yellow Flesh ◽  
High Level ◽  
Β Carotene

Lutein and zeaxanthin are becoming established as carotenoids beneficial for protection against common age-associated eye diseases. Thus, 15 potato (Solanum tuberosum subsp. tuberosum L.) breeding lines, cultivars Atlantic, Spunta, and Yukon Gold; and orange flesh OR-4 were surveyed for carotenoid profiles. Seven carotenoids, including violaxanthin, neoaxanthin, antheraxanthin, lutein, zeaxanthin, β-cryptoaxanthin, and β-carotene, were identified in the 19 genotypes. Violaxanthin and lutein were the prominent carotenoids detected in all genotypes studied. Neoaxanthin and antheraxanthin were found in 26% and 63% of the genotypes, respectively. β-Cryptoaxanthin, zeaxanthin, and β-carotene were found in only 5%, 10%, and 16% of the genotypes, respectively. Lutein varied from 19.8 to 119.0 μg·100 g-1 fresh weight across the 15 white- or yellow-flesh breeding lines. In contrast, zeaxanthin was detected at a low level in only one breeding line and at high level in OR-4. The three cultivars had profiles typical of yellow-flesh potatoes `Spunta' and `Yukon Gold'; while `Atlantic' had a typical white-flesh profile and a trace of zeaxanthin. The carotenoid baseline data established in this study provide information for activities to enhance potato for lutein and zeaxanthin.

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