Enhancement of poly-3-hydroxybutyrate (PHB) productivity by the two-stage supplementation of carbon sources and continuous feeding of NH4Cl

2008 ◽  
Vol 13 (1) ◽  
pp. 14-24 ◽  
Author(s):  
Joo Sang Yeo ◽  
Jae Yeon Park ◽  
Sung Ho Yeom ◽  
Young Je Yoo
Keyword(s):  
Carbon Sources ◽  
Two Stage ◽  
Continuous Feeding

Production of Bacterial Cellulose Using Different Carbon Sources by Two-Stage Cultivation Strategy

2011 ◽  
Vol 201-203 ◽  
pp. 722-725 ◽  
Author(s):  
Jun Hong Lin ◽  
Yi Jen Lin ◽  
Jui Chih Kuo ◽  
Ting Yu Chen ◽  
Wen Pei Sung
Keyword(s):  
Bacterial Cellulose ◽  
Reducing Sugars ◽  
Cell Concentration ◽  
Carbon Sources ◽  
Gluconacetobacter Xylinus ◽  
Glucose Medium ◽  
Two Stage ◽  
Second Stage ◽  
Cultivation Strategy ◽  
Cell Volumes

The production of bacterial cellulose (BC) from Gluconacetobacter xylinus could be improved by the two-stage cultivation strategy. The jar fermentor was applied at first stage to increase cell concentration. At the second stage, the bacteria statically grew within beaker. Three different fraction of cell volumes were cultured in modified YPD-glucose medium or YPD-molasses one. In the modified YPD-molasses medium, the BC yield were highest in this study when the consuming rate of reducing sugars (RS) was 0.181 g-dry BC L-1 d-1 and the BC production rate was 0.183 g-dry BC/g-RS, respectively. The modified YPD-glucose medium could get the maximum pellicle thickness of 5.56±0.64 mm and water content of 99.4%, respectively.

Two-Stage Continuous Feeding in the Production and Use of Carbon Nanomaterials

2018 ◽  
Vol 54 (5-6) ◽  
pp. 386-391 ◽  
Author(s):  
V. F. Pershin ◽  
A. M. Vorob’ev ◽  
V. M. Nechaev ◽  
A. A. Pas’ko ◽  
T. Kh. K. Alsaiyad
Keyword(s):  
Carbon Nanomaterials ◽  
Two Stage ◽  
Continuous Feeding

scholarly journals Post-Treatment of the Effluent from Anaerobic Digestion of the Leachate in Two-Stage SBR System Using Alternative Carbon Sources

2021 ◽  
Vol 13 (11) ◽  
pp. 6297
Author(s):  
Katarzyna Bernat ◽  
Dorota Kulikowska ◽  
Magdalena Zielińska ◽  
Magdalena Zaborowska ◽  
Irena Wojnowska-Baryła ◽  
...  
Keyword(s):  
Anaerobic Digestion ◽  
Nitrate Removal ◽  
Carbon Sources ◽  
Post Treatment ◽  
Effluent Treatment ◽  
Electricity Production ◽  
Nitrite Accumulation ◽  
Ammonium Oxidation ◽  
Two Stage ◽  
Removal Rates

Although anaerobic digestion (AD) enables biogas production and facilitates renewable electricity production, its effluent must be post-treated before discarding it into the environment. However, during AD designing, the post-treatment step is often overlooked. This paper presents the kinetics and efficiency of nitrogen removal from effluent after AD of leachate from the aerobic stabilization of the organic fraction of municipal solid waste. A two-stage SBR system was used. An ammonium oxidation rate of 15.5 mg N-NH4/(L·h) ensured a 98% nitrification efficiency (I stage). For denitrification (II stage), alternative carbon sources (ACS) (molasses, crude glycerine, or distillery stillage) were used. Two volumetric exchange rates (n) were tested: 0.35 1/d (COD/N-NO3 ratio of 8) and 0.5 1/d (COD/N-NO3 of 7). With all ACS and COD/N-NO3 ratios, almost 100% of nitrate was denitrified; at the COD/N-NO3 of 8, biodegradable organics remained in the effluents. At the COD/N-NO3 of 7, the denitrification removal rates were lower (29.6-45.1 mg N-NOx/(L·h)) than at the ratio of 8 (72.1–159.5 mg N-NOx/(L·h)), because of temporal nitrite accumulation. The highest nitrate removal rates were obtained with molasses, the lowest with a distillery stillage. Considering the nitrate removal rate and the effluent COD concentration, molasses was recommended as the most effective carbon source for AD effluent treatment at the COD/N-NO3 of 7.

Aerobic metabolism of mixed carbon sources in sequencing batch reactor under pulse and continuous feeding

2013 ◽  
Vol 129 ◽  
pp. 118-126 ◽  
Author(s):  
Asli S. Ciggin ◽  
Derin Orhon ◽  
Donatella Capitani ◽  
Alfredo Miccheli ◽  
Caterina Puccetti ◽  
...  
Keyword(s):  
Sequencing Batch Reactor ◽  
Batch Reactor ◽  
Carbon Sources ◽  
Aerobic Metabolism ◽  
Continuous Feeding

Continuous-Melter Vitrification of Fernald Mixed Wastes: Distribution of Contaminants Between Glass, Scrubber Solutions and Off-Gases

1993 ◽  
Vol 333 ◽  
Author(s):  
Keith S. Matlack ◽  
Hamid Hojaji ◽  
Sabrina S. Fu ◽  
Ian L. Pegg ◽  
Pedro B. Macedo
Keyword(s):  
Minimum Amount ◽  
Gaseous Emissions ◽  
Two Stage ◽  
Low Level ◽  
Regulatory Limits ◽  
Mixed Wastes ◽  
Solid Precipitate ◽  
Continuous Feeding

ABSTRACTResults are presented from treatability studies on the vitrification of hazardous and low-level radioactive soils and sludges from the Fernald site. The majority of the contaminants of concern are contained in large volumes of sludges accumulated from uranium and thorium production operations that are presently stored in pits at Fernald. These sludges present particular challenges to the vitrification process in that they contain significant amounts of fluorides (mostly magnesium and calcium). In this study, glass formulations used sludges, soils and a minimum amount of additives resulting in waste loadings as high as 94%. Several of these formulations were evaluated by continuous feeding of slurries into a joule-heated vitrification system. Melter off-gases were treated in a two-stage NaOH scrubbing system. HF acid fumes were neutralized, resulting in the formation of a NaF solid precipitate which is removed from the scrubber and recycled to subsequent melter feeds. Elemental and isotope compositions of the glasses, scrubber solutions, NaF precipitate and off-gases show that greater than 99.7% of uranium and thorium, 95% of Pb, Cd, Ni, Sb, Cr, and Hg were retained in the glass. Fluoride and other gaseous emissions from the scrubber system were below regulatory limits, even before HEPA filtration.

Two-stage self-seeding method for preparation of single crystals of poly(phenylene sulfide)

1989 ◽  
Vol 47 ◽  
pp. 368-369
Author(s):  
Sengshiu Chung ◽  
Peggy Cebe
Keyword(s):  
Single Crystals ◽  
High Performance ◽  
Dilute Solution ◽  
Two Stage ◽  
Annealing Behavior ◽  
High Performance Polymers ◽  
Seeding Method ◽  
Phenylene Sulfide

We are studying the crystallization and annealing behavior of high performance polymers, like poly(p-pheny1ene sulfide) PPS, and poly-(etheretherketone), PEEK. Our purpose is to determine whether PPS, which is similar in many ways to PEEK, undergoes reorganization during annealing. In an effort to address the issue of reorganization, we are studying solution grown single crystals of PPS as model materials.Observation of solution grown PPS crystals has been reported. Even from dilute solution, embrionic spherulites and aggregates were formed. We observe that these morphologies result when solutions containing uncrystallized polymer are cooled. To obtain samples of uniform single crystals, we have used two-stage self seeding and solution replacement techniques.

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