The optimal growth conditions for the biomass production of Isochrysis galbana and the effects that phosphorus, Zn2+, CO2, and light intensity have on the biochemical composition of Isochrysis galbana and the activity of extracellular CA

2009 ◽  
Vol 14 (2) ◽  
pp. 225-231 ◽  
Author(s):  
Yingying Sun ◽  
Changhai Wang
Keyword(s):  
Light Intensity ◽  
Biomass Production ◽  
Biochemical Composition ◽  
Isochrysis Galbana ◽  
Optimal Growth ◽  
Growth Conditions

scholarly journals Growth and biochemical composition of Chlorella vulgaris in different growth media

2013 ◽  
Vol 85 (4) ◽  
pp. 1427-1438 ◽  
Author(s):  
MATHIAS A. CHIA ◽  
ANA T. LOMBARDI ◽  
MARIA DA GRACA G. MELAO
Keyword(s):  
Biomass Production ◽  
Chlorella Vulgaris ◽  
Biochemical Composition ◽  
Physiological Response ◽  
Low Cost ◽  
Cost Effective ◽  
Growth Conditions ◽  
Growth Media ◽  
Continuous Cultures ◽  
The Cost

The need for clean and low-cost algae production demands for investigations on algal physiological response under different growth conditions. In this research, we investigated the growth, biomass production and biochemical composition of Chlorella vulgaris using semi-continuous cultures employing three growth media (LC Oligo, Chu 10 and WC media). The highest cell density was obtained in LC Oligo, while the lowest in Chu medium. Chlorophyll a, carbohydrate and protein concentrations and yield were highest in Chu and LC Oligo media. Lipid class analysis showed that hydrocarbons (HC), sterol esthers (SE), free fatty acids (FFA), aliphatic alcohols (ALC), acetone mobile polar lipids (AMPL) and phospholipids (PL) concentrations and yields were highest in the Chu medium. Triglyceride (TAG) and sterol (ST) concentrations were highest in the LC Oligo medium. The results suggested that for cost effective cultivation, LC Oligo medium is the best choice among those studied, as it saved the cost of buying vitamins and EDTA associated with the other growth media, while at the same time resulted in the best growth performance and biomass production.

Investigations on the photosynthetic sulfur bacterium Chlorobium phaeobacteroides causing seasonal blooms in Lake Kinneret

1979 ◽  
Vol 25 (9) ◽  
pp. 999-1007 ◽  
Author(s):  
T. Bergstein ◽  
Y. Henis ◽  
B. Z. Cavari
Keyword(s):  
Light Intensity ◽  
Photosynthetic Activity ◽  
Large Population ◽  
Optimal Growth ◽  
Growth Conditions ◽  
Organic Substrate ◽  
Lake Kinneret ◽  
Sulfur Bacteria ◽  
Chlorobium Phaeobacteroides ◽  
Photosynthetic Sulfur Bacteria

Between May and December, the annual stratification period in Lake Kinneret, sulfide is formed and accumulates in the hypolimnion. In July–August a large population (up to 106 cells/mL) of green, photosynthetic, sulfur bacteria develops at the boundary of the oxidative and reductive zones of the water column lasting for 3–8 weeks. These bacteria were isolated from the lake and identified as Chlorobium phaeobacteroides. Optimal growth conditions included 160 mg S=L−1 and light intensities of 5–0 μEinstein (μE) m−2s−1. Glucose and acetate augmented growth when added to the mineral medium. The lowest light intensity which still supported growth was 0.3 μE m−2s−1 when acetate was present and 1.0 μE m−2s−1 when no organic substrate was present. Under complete darkness, either with or without organic substrate, the bacteria die. Photosynthetic activity was higher when no organic compound was added to the medium. Uptake of acetate was light-dependant.In the lake the photosynthetic activity of the bacteria is low because of the limited light intensity (0.3 μE m−2s−1) at the bloom layer. It is suggested that the appearance and the disappearance of the bloom are caused by the influence of the daily internal seiche.

scholarly journals Effect of the deficiency of nitrate and silicate on the growth and composition of the benthic diatom Navicula incerta

2020 ◽  
Vol 48 (2) ◽  
pp. 280-286
Author(s):  
José Jesús Encinas-Arzate ◽  
Enrique Marquez-Ríos ◽  
José Antonio López-Elías ◽  
Wilfrido Torres-Areola ◽  
José Ángel Huerta-Ocampo ◽  
...  
Keyword(s):  
Growth Kinetics ◽  
Biochemical Composition ◽  
Optimal Growth ◽  
Growth Conditions ◽  
Benthic Diatom ◽  
Lipid Concentration ◽  
Silicate Concentration ◽  
Chlorophyll Protein ◽  
Potential Use ◽  
Navicula Incerta

Navicula incerta is a benthic diatom with potential use in nutrition and health for humans and aquaculture. Therefore, it is important to know its optimal growth conditions and biochemical composition. In this study, the effects of nitrate and silicate concentration on the growth kinetics and biochemical composition of N. incerta under laboratory conditions were measured. F/2 media was used as the control, and different concentrations of nitrogen (N/4, N/8 and N/16) and silicates (Si/4, Si/8 and Si/16) were evaluated. We measured cell concentration, chlorophyll-a and conducted proximal chemical analyses. It was found that different concentrations of nutrients affected the diatom’s growth kinetics and affected the concentrations of ash, chlorophyll, protein, lipids and carbohydrates. The highest concentration of lipids was obtained in the limiting treatment of nitrogen N/8 (27.09%), while the lowest value was found with silicate Si/8 media (16.97%). Carbohydrates increased compared to the control, with the N/16 treatment presenting the maximal concentration (23.31%). Treatments with reduced nitrate (N/8 and N/16) demonstrated the lowest concentrations of protein (18.75 and 12.44%, respectively), while in reduced silicate treatments, no statistical differences (P ≥ 0.05) were observed. Therefore, media limited nitrogen and silicates affected the growth kinetics and proximal chemical composition of N. incerta. The growth of this species using the N/8 medium is a suitable method for increasing lipid concentration in N. incerta.

scholarly journals Cultivation of Microalgae and Cyanobacteria: Effect of Operating Conditions on Growth and Biomass Composition

Molecules ◽  
2020 ◽  
Vol 25 (12) ◽  
pp. 2834 ◽  
Author(s):  
Alejandra Sánchez-Bayo ◽  
Victoria Morales ◽  
Rosalía Rodríguez ◽  
Gemma Vicente ◽  
Luis Fernando Bautista
Keyword(s):  
Cell Growth ◽  
Culture Media ◽  
Treatment Plant ◽  
Isochrysis Galbana ◽  
Volume Ratio ◽  
Optimal Growth ◽  
Growth Conditions ◽  
Operating Conditions ◽  
Biomass Composition ◽  
Nannochloropsis Gaditana

The purpose of this work is to define optimal growth conditions to maximise biomass for batch culture of the cyanobacterium Arthrospira maxima and the microalgae Chlorella vulgaris, Isochrysis galbana and Nannochloropsis gaditana. Thus, we study the effect of three variables on cell growth: i.e., inoculum:culture medium volume ratio (5:45, 10:40, 15:35 and 20:30 mL:mL), light:dark photoperiod (8:16, 12:12 and 16:8 h) and type of culture medium, including both synthetic media (Guillard’s F/2 and Walne’s) and wastewaters. The results showed that the initial inoculum:culture medium volume ratio, within the range 5:45 to 20:30, did not affect the amount of biomass at the end of the growth (14 days), whereas high (18 h) or low (6 h) number of hours of daily light was important for cell growth. The contribution of nutrients from different culture media could increase the growth rate of the different species. A. maxima was favoured in seawater enriched with Guillard’s F/2 as well as C. vulgaris and N. gaditana, but in freshwater medium. I. galbana had the greatest growth in the marine environment enriched with Walne’s media. Nitrogen was the limiting nutrient for growth at the end of the exponential phase of growth for C. vulgaris and N. gaditana, while iron was for A. maxima and I. galbana. The growth in different synthetic culture media also determines the biochemical composition of each of the microalgae. All species demonstrated their capability to grow in effluents from a wastewater treatment plant and they efficiently consume nitrogen, especially the three microalga species.

scholarly journals Effect of Cultivation Variables on Biomass Composition and Growth of Microalgae and Cyanobacteria

2019 ◽  
Author(s):  
Alejandra Sánchez-Bayo ◽  
Victoria Morales ◽  
Rosalía Rodríguez ◽  
Gemma Vicente Crespo ◽  
Luis Fernando Bautista
Keyword(s):  
Culture Media ◽  
Treatment Plant ◽  
Isochrysis Galbana ◽  
Optimal Growth ◽  
Growth Conditions ◽  
Biomass Composition ◽  
Water Medium ◽  
Nannochloropsis Gaditana ◽  
Initial Inoculum ◽  
Inoculum Volume

The purpose of this work is to define optimal growth conditions for batch culture of the cyanobacterium Arthrospira maxima and the microalgae Chlorella vulgaris, Isochrysis galbana and Nannochloropsis gaditana. Thus, we study the effect of three variables on algae growth: i.e., inoculum:culture medium ratio, light:darkness photoperiod and type of culture medium, including both synthetic media and wastewaters. The results showed that the initial inoculum volume did not affect the amount of biomass at the end of the growth (14 days), whereas an excess (18 h) or defect (6 h) in the number of hours of light is determinant for its development. The contribution of nutrients from different culture media modified the growth of the different species. A. maxima was favoured in seawater enriched with Guillard's F/2 as well as C. vulgaris and N. gaditana but in fresh water medium. I. galbana had the greatest growth in the marine environment enriched with Walne’s media. Nitrate was the limiting growth reagent at the end of the exponential phase of growth for C. vulgaris and N. gaditana, while iron was for A. maxima and I. galbana. All species demonstrated their capability to grow in effluents from a wastewater treatment plant and they efficiently consume nitrogen, especially the three microalgae species.

Optimal growth conditions for Isochrysis galbana

Biomass ◽  
1986 ◽  
Vol 9 (1) ◽  
pp. 37-48 ◽  
Author(s):  
Drora Kaplan ◽  
Zvi Cohen ◽  
Aharon Abeliovich
Keyword(s):  
Isochrysis Galbana ◽  
Optimal Growth ◽  
Growth Conditions
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