Microalgal Biomass and Lipid Production on Dairy Effluent Using a Novel Microalga, Chlorella sp. Isolated from Dairy Wastewater

2018 ◽  
Vol 23 (3) ◽  
pp. 333-340 ◽  
Author(s):  
Yong-Keun Choi ◽  
Hyun Min Jang ◽  
Eunsung Kan
Keyword(s):  
Lipid Production ◽  
Dairy Wastewater ◽  
Microalgal Biomass ◽  
Dairy Effluent ◽  
Chlorella Sp

scholarly journals Microalgae Cultivation in Different pH, Temperature and Media for Lipid Production

2014 ◽  
Vol 8 (2) ◽  
pp. 13-17 ◽  
Author(s):  
Dakshayini Jayaramareddy ◽  
Ravikumar Krishnappa ◽  
Girisha Sirangala Thimmappa
Keyword(s):  
Lipid Production ◽  
Life Sciences ◽  
Nutrient Supply ◽  
Polar Lipids ◽  
Microalgae Cultivation ◽  
Microalgal Biomass ◽  
Chlorella Sp ◽  
Nonpolar Lipids

Lipids produced by microalgal biomass can be grouped into nonpolar lipids and polar lipids, which can be easily converted into biofuels. Microalgal samples were collected from three different ponds of Bangalore and cultured in the laboratory to find the effect of different pH, temperature and media on the production of biomass and lipids. Among these, pH-9, temperature -25°C and Beneck’s media was most suitable for production of biomass (35.80 g/L) and lipids from the isolated microalgae Chlorella sp. compare to Chladospora sp. (13.33 g/L). Chlorella sp. Showed 0.32 (OD) at pH-9, 0.43 (OD) at temperature-25°C and 2.94 (OD) in Beneck’s media. Our result revealed that nutrient supply along with measured variables affects the production of biomass and lipids in different microalgae.DOI: http://dx.doi.org/10.3126/ijls.v8i2.10227 International Journal of Life Sciences Vol.8(2): 2014; 13-17

Lipid Production of Heterotrophic Chlorella sp. from Hydrolysate Mixtures of Lipid-Extracted Microalgal Biomass Residues and Molasses

2015 ◽  
Vol 177 (3) ◽  
pp. 662-674 ◽  
Author(s):  
Hongli Zheng ◽  
Xiaochen Ma ◽  
Zhen Gao ◽  
Yiqin Wan ◽  
Min Min ◽  
...  
Keyword(s):  
Lipid Production ◽  
Microalgal Biomass ◽  
Biomass Residues ◽  
Chlorella Sp

scholarly journals Metabolomic Study of Heterotrophically Grown Chlorella sp. Isolated from Wastewater in Northern Sweden

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2410
Author(s):  
Jean Claude Nzayisenga ◽  
Anita Sellstedt
Keyword(s):  
Sustainable Development ◽  
Butyric Acid ◽  
Lipid Production ◽  
Growth Conditions ◽  
Northern Sweden ◽  
Metabolic Intermediate ◽  
Chlorella Sp ◽  
Lipid Contents ◽  
New Compounds ◽  
Potential Use

There are numerous strains of Chlorella with a corresponding variety of metabolic pathways. A strain we previously isolated from wastewater in northern Sweden can grow heterotrophically as well as autotrophically in light and has higher lipid contents under heterotrophic growth conditions. The aims of the present study were to characterize metabolic changes associated with the higher lipid contents in order to enhance our understanding of lipid production in microalgae and potentially identify new compounds with utility in sustainable development. Inter alia, the amino acids glutamine and lysine were 7-fold more abundant under heterotrophic conditions, the key metabolic intermediate alpha-ketoglutarate was more abundant under heterotrophic conditions with glucose, and maltose was more abundant under heterotrophic conditions with glycerol than under autotrophic conditions. The metabolite 3-hydroxy-butyric acid, the direct precursor of the biodegradable plastic PHB (poly-3-hydroxy-butyric acid), was also more abundant under heterotrophic conditions. Our metabolomic analysis has provided new insights into the alga’s lipid production pathways and identified metabolites with potential use in sustainable development, such as the production of renewable, biodegradable plastics, cosmetics, and nutraceuticals, with reduced pollution and improvements in both ecological and human health.

scholarly journals 244 Algae taste preference of Chlorella sp. from dairy wastewater by weaned dairy calves

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 15-15
Author(s):  
Bradley J Heins ◽  
Siane C Luzzi ◽  
Robert D Gardner
Keyword(s):  
Dry Matter ◽  
Taste Preference ◽  
Dairy Wastewater ◽  
Control Treatment ◽  
Central Research ◽  
Solar Panels ◽  
University Of Minnesota ◽  
Chlorella Sp ◽  
Coefficient Of Concordance ◽  
The Difference

Abstract The objective of this study was to evaluate the taste preference of calves fed Chlorella sp. microalgae produced from dairy lagoon wastewater. The study was conducted at the University of Minnesota West Central Research and Outreach Center, Morris, MN, dairy during May 2019. Six Holstein and crossbred dairy heifer calves were fed 0 (control), 30, and 60 g of Chlorella sp. daily in a sequential elimination study. For the 7-d experiment, day 1 to 2 were for diet adaptation and day 3 to 4 were for data collection. During the final 3 days, the primarily consumed treatment was removed to determine the second preferred treatment. The microalgae used in this study was isolated from the dairy wastewater lagoon. The microalgae biomass was produced using outdoor hanging bag bioreactors with Chlorella sp. to recycle the dairy wastewater. The biomass was sterilized and kept frozen at -4°C until fed to calves. Calves were housed individually in hutches with outdoor access under solar panels, with free-choice water. Kendall’s coefficient of concordance was calculated to rank the consumption of the treatments from most to least preferred using JMP 14.3 statistical software. Pairwise comparisons and Tukey adjustment were applied to evaluate the difference between the treatments for total intake. Calves consumed more (P < 0.05) dry matter from control grain (3.4 kg/d) compared to 30 g microalgae grain (2.42 kg/d) and 60 g microalgae grain (1.56 kg/d) during the first 2-d period. During the second 2-d (d 3 and 4) segment, dry matter intake was reduced (P < 0.05) for the 60 g microalgae treatment compared to the control and 30 g microalgae treatment. Five of six calves in this study always ranked the control treatment first (P < 0.05) when given a choice, and ranked the 30 g microalgae second choice. Results indicated that microalgae maybe added to calf starter grains without any adverse effects; however, calves preferred calf starter grains without microalgae.

Cultivation of Chlorella sp. with livestock waste compost for lipid production

2017 ◽  
Vol 223 ◽  
pp. 296-300 ◽  
Author(s):  
L.-D. Zhu ◽  
Z.-H. Li ◽  
D.-B. Guo ◽  
F. Huang ◽  
Y. Nugroho ◽  
...  
Keyword(s):  
Lipid Production ◽  
Livestock Waste ◽  
Chlorella Sp

Process Engineering for High-Cell-Density Cultivation of Lipid Rich Microalgal Biomass of Chlorella sp. FC2 IITG

2014 ◽  
Vol 8 (2) ◽  
pp. 726-739 ◽  
Author(s):  
Muthusivaramapandian Muthuraj ◽  
Niharika Chandra ◽  
Basavaraj Palabhanvi ◽  
Vikram Kumar ◽  
Debasish Das
Keyword(s):  
Cell Density ◽  
High Cell Density ◽  
Process Engineering ◽  
High Cell ◽  
High Cell Density Cultivation ◽  
Microalgal Biomass ◽  
Chlorella Sp

scholarly journals The Dark Side of Microalgae Biotechnology: A Heterotrophic Biorefinery Platform Directed to ω-3 Rich Lipid Production

2019 ◽  
Vol 7 (12) ◽  
pp. 670 ◽  
Author(s):  
Teresa Lopes da Silva ◽  
Patrícia Moniz ◽  
Carla Silva ◽  
Alberto Reis
Keyword(s):  
Low Cost ◽  
Lipid Production ◽  
Arable Land ◽  
Integrated System ◽  
Dark Side ◽  
Omega 3 ◽  
Niche Markets ◽  
Microalgal Biomass ◽  
Oleaginous Microalgae ◽  
Heterotrophic Microalgae

Microbial oils have been considered a renewable feedstock for bioenergy not competing with food crops for arable land, freshwater and biodiverse natural landscapes. Microalgal oils may also have other purposes (niche markets) besides biofuels production such as pharmaceutical, nutraceutical, cosmetic and food industries. The polyunsaturated fatty acids (PUFAs) obtained from oleaginous microalgae show benefits over other PUFAs sources such as fish oils, being odorless, and non-dependent on fish stocks. Heterotrophic microalgae can use low-cost substrates such as organic wastes/residues containing carbon, simultaneously producing PUFAs together with other lipids that can be further converted into bioenergy, for combined heat and power (CHP), or liquid biofuels, to be integrated in the transportation system. This review analyses the different strategies that have been recently used to cultivate and further process heterotrophic microalgae for lipids, with emphasis on omega-3 rich compounds. It also highlights the importance of studying an integrated process approach based on the use of low-cost substrates associated to the microalgal biomass biorefinery, identifying the best sustainability methodology to be applied to the whole integrated system.

scholarly journals Statistical Approach of Nutrient Optimization for Microalgae Cultivation

2020 ◽  
Vol 141 ◽  
pp. 03009
Author(s):  
Pichayatorn Bunkaew ◽  
Sasithorn Kongruang
Keyword(s):  
Light Intensity ◽  
Cobalt Chloride ◽  
Lipid Production ◽  
Biodiesel Production ◽  
Mixotrophic Cultivation ◽  
Microalgae Cultivation ◽  
Cultivation Time ◽  
Chlorella Sp ◽  
Chloride Hexahydrate ◽  
Cobalt Chloride Hexahydrate

The Plackett-Burman Design (PBD) was applied to study fresh water microalgae cultivation using Chlorella sp. TISTR 8411 to select the influential nutrient factors for biomass and lipid production. The PBD for 13 trials from 11 nutrient factors with 3 levels was studied in the mixotrophic cultivation at 28 0C under 16:8 light and dark photoperiods over 7 days of cultivation time. Two influential factors were chosen as glucose and cobalt chloride hexahydrate to further design via Box-Behnken Design (BBD) in order to optimize the cultivation of this microalgae for biodiesel production. The 17 trials of 3 factors and 3 levels of BBD experimental design technique were applied with varying factors of glucose (20-40 g/L), cobalt chloride hexahydrate (0.01-0.04 mg/L) and light intensity (4,500-7,500 Lux) under 16:8 light and dark photoperiods over 7 days of cultivation time at 28 0C. Result showed that Chlorella sp. TISTR 8411 cultivation yield 0.52 g/L biomass and 0.31 g/L lipid production resulting in approximately 60% of lipid production when cultivated in 20.05 g/L glucose, 0.04 mg/L CoCl26H2O under light intensity of 4,614 Lux with the supplementation of 4.38 g/L NaHCO3 coupled with 1 g/L of both NaNO3 and KH2PO4. Under statically mixotrophic cultivation, result indicated that Chlorella sp. TISTR 8411 had potential to produce high lipid content for biodiesel application and biomass production for nutraceutical application. Further experiment with the longer cultivation period up to 2 weeks would implement not only for monitoring the growth kinetics but also evaluating the suitable type of fatty acid production.

Bioremediation of Pyropia-processing wastewater coupled with lipid production using Chlorella sp.

2021 ◽  
Vol 321 ◽  
pp. 124428
Author(s):  
Shiyan Zheng ◽  
Shanyi Chen ◽  
Shangyun Zou ◽  
Yiwen Yan ◽  
Guang Gao ◽  
...  
Keyword(s):  
Lipid Production ◽  
Chlorella Sp
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