Significance of Adopting Nucleic Acid Amplification Technique for Blood Donor Screening in a Resource Limited Setting: A Study from a Single Centre in South India

2021 ◽  
Author(s):  
Sudha Ranganathan ◽  
Ranganathan N. Iyer ◽  
Nagalla Balakrishna
Keyword(s):  
Nucleic Acid ◽  
Blood Donor ◽  
South India ◽  
Nucleic Acid Amplification ◽  
Single Centre ◽  
Resource Limited ◽  
Blood Donor Screening ◽  
Amplification Technique ◽  
Nucleic Acid Amplification Technique ◽  
Limited Setting

scholarly journals Risk Minimization Measures for Blood Screening HIV-1 Nucleic Acid Amplification Technique Assays in Germany

2014 ◽  
Vol 41 (1) ◽  
pp. 8-8 ◽  
Author(s):  
Michael Chudy ◽  
Julia Kress ◽  
Jochen Halbauer ◽  
Margarethe Heiden ◽  
Markus B. Funk ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acid Amplification ◽  
Risk Minimization ◽  
Amplification Technique ◽  
Hiv 1 ◽  
Nucleic Acid Amplification Technique ◽  
Blood Screening

Quantitative nucleic acid amplification by digital PCR for clinical viral diagnostics

2016 ◽  
Vol 0 (0) ◽  
Author(s):  
Kuo Zhang ◽  
Guigao Lin ◽  
Jinming Li
Keyword(s):  
Nucleic Acid ◽  
Digital Pcr ◽  
Nucleic Acid Amplification ◽  
Target Sequence ◽  
The Past ◽  
Amplification Technique ◽  
Diagnostic Applications ◽  
Further Development ◽  
Nucleic Acid Amplification Technique ◽  
Viral Diagnostics

AbstractIn the past few years, interest in the development of digital PCR (dPCR) as a direct nucleic acid amplification technique for clinical viral diagnostics has grown. The main advantages of dPCR over qPCR include: quantification of nucleic acid concentrations without a calibration curve, comparable sensitivity, superior quantitative precision, greater resistance to perturbations by inhibitors, and increased robustness to the variability of the target sequence. In this review, we address the application of dPCR to viral nucleic acid quantification in clinical applications and for nucleic acid quantification standardization. Further development is required to overcome the current limitations of dPCR in order to realize its widespread use for viral load measurements in clinical diagnostic applications.

Investigation of an algorithm for anti HCV EIA reactivity in blood donor screening in Turkey in the absence of nucleic acid amplification screening

2017 ◽  
Vol 56 (5) ◽  
pp. 732-737 ◽  
Author(s):  
Ayse Esra Karakoc ◽  
Rukiye Berkem ◽  
Hasan Irmak ◽  
Ali Pekcan Demiroz ◽  
Idil Yenicesu ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Blood Donor ◽  
Nucleic Acid Amplification ◽  
Donor Screening ◽  
Blood Donor Screening

scholarly journals SIMPLIFIED DIAGNOSIS OF MALARIA INFECTION: GFM/PCR/ELISA A SIMPLIFIED NUCLEIC ACID AMPLIFICATION TECHNIQUE BY PCR/ELISA

1998 ◽  
Vol 40 (5) ◽  
pp. 333-334 ◽  
Author(s):  
Ricardo Luiz Dantas MACHADO ◽  
Denise Oliveira GARRET ◽  
Ipemida Sullayman ADAGU ◽  
David Charles WARHURST ◽  
Marinete Marins PÓVOA
Keyword(s):  
Nucleic Acid ◽  
Genetic Analysis ◽  
Blood Sample ◽  
Malaria Infection ◽  
Nucleic Acid Amplification ◽  
Sample Collection ◽  
Amplification Technique ◽  
The Cost ◽  
Nucleic Acid Amplification Technique

We report an adaptation of a technique for the blood sample collection (GFM) as well as for the extraction and amplification of Plasmodium DNA for the diagnosis of malaria infection by the PCR/ELISA. The method of blood sample collection requires less expertise and saves both time and money, thus reducing the cost by more than half. The material is also suitable for genetic analysis in either fresh or stored specimens prepared by this method.

A netlike rolling circle nucleic acid amplification technique

The Analyst ◽  
2015 ◽  
Vol 140 (1) ◽  
pp. 74-78 ◽  
Author(s):  
Xiaoli Zhu ◽  
Chang Feng ◽  
Bin Zhang ◽  
Hui Tong ◽  
Tao Gao ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Rolling Circle Amplification ◽  
Nucleic Acid Amplification ◽  
Amplification Efficiency ◽  
Rolling Circle ◽  
Isothermal Nucleic Acid Amplification ◽  
Amplification Technique ◽  
Nucleic Acid Amplification Technique ◽  
Network Morphology

An isothermal nucleic acid amplification technique termed as netlike rolling circle amplification is proposed. Dense and uniform network morphology of amplified products is first observed, suggesting the ultrahigh amplification efficiency.

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