scholarly journals A proteomic study of cysteine protease induced cell death in anthers of male sterile tobacco transgenic plants

2019 ◽  
Vol 25 (4) ◽  
pp. 1073-1082 ◽  
Author(s):  
Pawan Shukla ◽  
Ranjana Gautam ◽  
Naveen Kumar Singh ◽  
Israr Ahmed ◽  
Pulugurtha Bharadwaja Kirti
Keyword(s):  
Cell Death ◽  
Transgenic Plants ◽  
Cysteine Protease ◽  
Male Sterile ◽  
Proteomic Study ◽  
Tobacco Transgenic Plants

scholarly journals The Cysteine Protease CEP1, a Key Executor Involved in Tapetal Programmed Cell Death, Regulates Pollen Development in Arabidopsis

2014 ◽  
Vol 26 (7) ◽  
pp. 2939-2961 ◽  
Author(s):  
Dandan Zhang ◽  
Di Liu ◽  
Xiaomeng Lv ◽  
Ying Wang ◽  
Zhili Xun ◽  
...  
Keyword(s):  
Cell Death ◽  
Programmed Cell Death ◽  
Cysteine Protease ◽  
Pollen Development

scholarly journals Expression of an Oxalate Decarboxylase Impairs the Necrotic Effect Induced by Nep1-like Protein (NLP) of Moniliophthora perniciosa in Transgenic Tobacco

2011 ◽  
Vol 24 (7) ◽  
pp. 839-848 ◽  
Author(s):  
Leonardo F. da Silva ◽  
Cristiano V. Dias ◽  
Luciana C. Cidade ◽  
Juliano S. Mendes ◽  
Carlos P. Pirovani ◽  
...  
Keyword(s):  
Cell Death ◽  
Transgenic Plants ◽  
Plant Pathogens ◽  
Oxygen Species ◽  
Oxalate Decarboxylase ◽  
Moniliophthora Perniciosa ◽  
Enhanced Resistance ◽  
Broom Disease ◽  
Ros Formation ◽  
Theobroma Cacao L

Oxalic acid (OA) and Nep1-like proteins (NLP) are recognized as elicitors of programmed cell death (PCD) in plants, which is crucial for the pathogenic success of necrotrophic plant pathogens and involves reactive oxygen species (ROS). To determine the importance of oxalate as a source of ROS for OA- and NLP-induced cell death, a full-length cDNA coding for an oxalate decarboxylase (FvOXDC) from the basidiomycete Flammulina velutipes, which converts OA into CO2 and formate, was overexpressed in tobacco plants. The transgenic plants contained less OA and more formic acid compared with the control plants and showed enhanced resistance to cell death induced by exogenous OA and MpNEP2, an NLP of the hemibiotrophic fungus Moniliophthora perniciosa. This resistance was correlated with the inhibition of ROS formation in the transgenic plants inoculated with OA, MpNEP2, or a combination of both PCD elicitors. Taken together, these results have established a pivotal function for oxalate as a source of ROS required for the PCD-inducing activity of OA and NLP. The results also indicate that FvOXDC represents a potentially novel source of resistance against OA- and NLP-producing pathogens such as M. perniciosa, the causal agent of witches' broom disease of cacao (Theobroma cacao L.).

scholarly journals Production of male sterile transgenic plants

2005 ◽  
Vol 22 (5) ◽  
pp. 469-476 ◽  
Author(s):  
Keita Takada ◽  
Hiroshi Kamada ◽  
Hiroshi Ezura
Keyword(s):  
Transgenic Plants ◽  
Male Sterile

scholarly journals Intracellular Staphylococcus aureus employs the cysteine protease staphopain A to induce host cell death in epithelial cells

2020 ◽  
Author(s):  
Kathrin Stelzner ◽  
Tobias Hertlein ◽  
Aneta Sroka ◽  
Adriana Moldovan ◽  
Kerstin Paprotka ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Cell Death ◽  
Epithelial Cells ◽  
Host Cell ◽  
Cysteine Protease ◽  
Host Cells ◽  
Upper Respiratory Tract ◽  
Intracellular Replication ◽  
Tissue Destruction ◽  
Host Cell Death

AbstractStaphylococcus aureus is a major human pathogen, which can invade and survive in non-professional and professional phagocytes. Intracellularity is thought to contribute to pathogenicity and persistence of the bacterium. Upon internalization by epithelial cells, cytotoxic S. aureus strains can escape from the phagosome, replicate in the cytosol and induce host cell death. Here, we identified a staphylococcal cysteine protease to induce cell death by intracellular S. aureus after translocation into the host cell cytoplasm. We demonstrated that loss of staphopain A function leads to delayed onset of host cell death and prolonged intracellular replication of S. aureus in epithelial cells. Overexpression of staphopain A in a non-cytotoxic strain facilitated intracellular killing of the host cell even in the absence of detectable intracellular replication. Moreover, staphopain A contributed to efficient colonization of the lung in a mouse pneumonia model. Our study suggests that staphopain A is utilized by S. aureus to mediate escape from the host cell and thus contributes to tissue destruction and dissemination of infection.Author SummaryStaphylococcus aureus is a well-known antibiotic-resistant pathogen that emerges in hospital and community settings and can cause a variety of diseases ranging from skin abscesses to lung inflammation and blood poisoning. The bacterium asymptomatically colonizes the upper respiratory tract and skin of about one third of the human population and takes advantage of opportune conditions, like immunodeficiency or breached barriers, to cause infection. Although S. aureus is not regarded as a professional intracellular bacterium, it can be internalized by human cells and subsequently exit the host cells by induction of cell death, which is considered to cause tissue destruction and spread of infection. The bacterial virulence factors and underlying molecular mechanisms involved in the intracellular lifestyle of S. aureus remain largely unknown. We identified a bacterial cysteine protease to contribute to host cell death mediated by intracellular S. aureus. Staphopain A induced killing of the host cell after translocation of the pathogen into the cell cytosol, while bacterial proliferation was not required. Further, the protease enhanced survival of the pathogen during lung infection. These findings reveal a novel, intracellular role for the bacterial protease staphopain A.

scholarly journals DCET1 Controls Male Sterility Through Callose Regulation, Exine Formation, and Tapetal Programmed Cell Death in Rice

2021 ◽  
Vol 12 ◽  
Author(s):  
Riaz Muhammad Khan ◽  
Ping Yu ◽  
Lianping Sun ◽  
Adil Abbas ◽  
Liaqat Shah ◽  
...  
Keyword(s):  
Cell Death ◽  
Programmed Cell Death ◽  
Male Sterility ◽  
Tapetal Cell ◽  
Pollen Wall ◽  
Rna Recognition Motif ◽  
Male Sterile ◽  
Cell Degeneration ◽  
Regulatory Pathways ◽  
Callose Wall

In angiosperms, anther development comprises of various complex and interrelated biological processes, critically needed for pollen viability. The transitory callose layer serves to separate the meiocytes. It helps in primexine formation, while the timely degradation of tapetal cells is essential for the timely callose wall dissolution and pollen wall formation by providing nutrients for pollen growth. In rice, many genes have been reported and functionally characterized that are involved in callose regulation and pollen wall patterning, including timely programmed cell death (PCD) of the tapetum, but the mechanism of pollen development largely remains ambiguous. We identified and functionally characterized a rice mutant dcet1, having a complete male-sterile phenotype caused by defects in anther callose wall, exine patterning, and tapetal PCD. DCET1 belongs to the RNA recognition motif (RRM)-containing family also called as the ribonucleoprotein (RNP) domain or RNA-binding domain (RBD) protein, having single-nucleotide polymorphism (SNP) substitution from G (threonine-192) to A (isoleucine-192) located at the fifth exon of LOC_Os08g02330, was responsible for the male sterile phenotype in mutant dcet1. Our cytological analysis suggested that DCET1 regulates callose biosynthesis and degradation, pollen exine formation by affecting exine wall patterning, including abnormal nexine, collapsed bacula, and irregular tectum, and timely PCD by delaying the tapetal cell degeneration. As a result, the microspore of dcet1 was swollen and abnormally bursted and even collapsed within the anther locule characterizing complete male sterility. GUS and qRT-PCR analysis indicated that DCET1 is specifically expressed in the anther till the developmental stage 9, consistent with the observed phenotype. The characterization of DCET1 in callose regulation, pollen wall patterning, and tapetal cell PCD strengthens our knowledge for knowing the regulatory pathways involved in rice male reproductive development and has future prospects in hybrid rice breeding.

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