The Effects of NH4+ and NO3− and Plant Growth Regulators on the Accumulation of Nutrients, Carbohydrates and Secondary Metabolites of Stevia rebaudiana Bertoni

Sugar Tech ◽  
2020 ◽  
Author(s):  
Hourieh Tavakoli Hasanaklou ◽  
Ali Ebadi Khazineh Ghadim ◽  
Foad Moradi ◽  
Sodabeh Jahanbakhsh Ghodehkahriz ◽  
Abdolghayoum Gholipouri
Keyword(s):  
Secondary Metabolites ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Stevia Rebaudiana ◽  
Stevia Rebaudiana Bertoni

scholarly journals Micropropagation of Stevia rebaudiana plants

2020 ◽  
Vol 50 (1) ◽  
Author(s):  
Marta Teresa Rokosa ◽  
Danuta Kulpa
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Stevia Rebaudiana ◽  
In Vitro Cultures ◽  
Optimum Composition ◽  
Ms Medium ◽  
Single Node ◽  
Stevia Rebaudiana Bertoni

ABSTRACT: The aim of the study was to develop optimum composition of plant growth regulators in media for the propagation and rooting of shoots of stevia (Stevia rebaudiana Bertoni) in in vitro cultures. Single-node shoot fragments obtained from plants propagated on MS medium were placed onto media supplemented with: BAP, 2iP and KIN at concentrations: 0.5, 1, 2 and 5 mg∙dm-3, whereas at the rooting stage with addition of: IAA, IBA and NAA at concentrations 1, 2, 4 and 8 mg∙dm-3. The highest number of shoots and leaves was reported for plants propagated on MS medium enriched with 0.5 mg∙dm-3 BAP. The greatest number of the longest roots was developed by stevia on the MS medium enriched with 1 mg∙dm-3 IAA.

Differential effects of plant growth regulators on physiology, steviol glycosides content, and antioxidant capacity in micropropagated tissues of Stevia rebaudiana

Biologia ◽  
2017 ◽  
Vol 72 (10) ◽  
Author(s):  
Rabia Javed ◽  
Buhara Yucesan ◽  
Muhammad Zia ◽  
Ekrem Gurel
Keyword(s):  
Secondary Metabolites ◽  
Plant Growth ◽  
Antioxidant Capacity ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Biotic Stress ◽  
Stevia Rebaudiana ◽  
Steviol Glycosides ◽  
Defence Mechanism ◽  
Differential Effects

AbstractPlants naturally produce secondary metabolites on exposure to abiotic/biotic stress. Production of secondary metabolites, like phenols or flavonoids, is a defence mechanism against different stresses. This study explores the influence of plant growth regulators (PGRs) on the growth and secondary metabolites of

scholarly journals Micropropagation of Stevia rebaudiana Bertoni Using RITA® Bioreactor

HortScience ◽  
2019 ◽  
Vol 54 (4) ◽  
pp. 725-731 ◽  
Author(s):  
Meltem Bayraktar
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Woody Plant ◽  
Stevia Rebaudiana ◽  
Root Induction ◽  
Culture Systems ◽  
Stevia Rebaudiana Bertoni ◽  
Woody Plant Medium ◽  
One Step

Stevia rebaudiana Bertoni is used for medicinal and food purposes. The effectiveness of different culture systems in Stevia micropropagation was evaluated. Node explants were cultured in semisolid or RITA® bioreactor containing woody plant medium (WPM) without plant growth regulators (PGRs). The effect of three medium volumes (100, 200, and 300 mL) and four immersion frequencies (10 seconds every 1, 4, 6, or 8 hours) were assessed. The shoots and roots developed simultaneously in the RITA® bioreactor treatment containing 100 mL of WPM at 10 seconds/4 hours immersion frequency and the healthiest plantlets were obtained from this RITA® treatment using 5 mL of medium per explant. The root induction rate was 100% in nonhyperhydrated shoots obtained from both semisolid and RITA® bioreactor, and all of them were successfully acclimatized. Thus, an economical and viable protocol was developed by performing the micropropagation in one step, eliminating the use of agar and PGRs, and decreasing the amount of medium used per explant.

scholarly journals THE ROLL OF SOME PLANT GROWTH REGULATORS ON SHOOTS MULTIPLICATION OF STEVIA PLANTS IN VITRO

2017 ◽  
Vol 48 (5) ◽  
Author(s):  
Al-Obaidy & Khierallah
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Culture Media ◽  
Stevia Rebaudiana ◽  
Shoot Multiplication ◽  
Dry Weight ◽  
Fresh Weight ◽  
Number Of Shoots

This research was conducted to study the effect of some plant growth regulators on in vitro shoots multiplication of stevia (Stevia rebaudiana Bertoni). The experiments included tests of various combinations of KIN with IBA or IAA in the shoot multiplication. Results indicated that KIN at 1.0 mg. L-1 plus 0.3 mg. L-1 of IBA produced the highest number of shoots (3.5 shoots) while KIN at 1.5 mg. L-1 plus IBA at 1.0 mg. L-1 produced the lowest shoot length (1.14 cm).  Hormone free medium produced the highest rate of the leaves number reached 28.56 leaves. KIN and IBA interaction increased fresh and dry weight significantly.   Treatment contained 2.0 mg -1 KIN plus 0.3 mg. L-1 IBA produced the highest fresh weight (1.739 g) while 0.5 mg. L-1 KIN and 0.3 mg. L-1 IBA produced the highest dry weight (0.822 g). As for the effect of interaction between the IAA and KIN it was significant in the number of shoots formed. Interaction between 1.0 mg. L-1 KIN with 0.1 mg. L-1IAA produced the highest number of shoots (3.8 shoots). Shoots length reached 8.10 cm in the media with 0.3 mg. L-1 IAA only. The highest fresh weight (1.267 g) was achieved with the interaction between 1.0 mg. L-1 KIN and 0.3 mg. L-1 IAA while 0.5 mg. L-1IAA without KIN produced the highest dry weight reached 0.138 g.  Shoots multiplication was improved by incorporation of the cytokinin TDZ in culture media. Shoots number, fresh and dry weights were increased significantly by adding 0.05 mg. L-1 of TDZ at present of 0.3 mg. L-1 of IBA giving 6.6 shoots, 0.974 g and 0.144 g respectively while shoots length decreased significantly as media without TDZ produced the highest shoots length reached 9.32 cm. The above results can adopt for the successful in vitro shoot multiplication of Stevia plants. 

Plant growth regulators affecting in vitro cultivation of Stevia rebaudiana

Sugar Tech ◽  
2008 ◽  
Vol 10 (3) ◽  
pp. 254-259 ◽  
Author(s):  
Ibrahim A. Ibrahim ◽  
Mahmoud I. Nasr ◽  
Berlanti R. Mohammed ◽  
Mohammed M. El-Zefzafi
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Stevia Rebaudiana ◽  
In Vitro Cultivation
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