Intracerebroventricular injection with octanoic acid activates hypothalamic fatty acid sensing systems and regulates appetite in Chinese perch Siniperca chuatsi

2022 ◽  
Author(s):  
Hexiong Feng ◽  
Di Peng ◽  
Xu-Fang Liang ◽  
Jiao Li ◽  
Haocan Luo ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Octanoic Acid ◽  
Intracerebroventricular Injection ◽  
Siniperca Chuatsi ◽  
Sensing Systems ◽  
Chinese Perch ◽  
Fatty Acid Sensing

scholarly journals Effects of CCK-8 and GLP-1 on fatty acid sensing and food intake regulation in trout

2019 ◽  
Vol 62 (3) ◽  
pp. 101-116
Author(s):  
Cristina Velasco ◽  
Sara Comesaña ◽  
Marta Conde-Sieira ◽  
Jesús M Míguez ◽  
José L Soengas
Keyword(s):  
Fatty Acid ◽  
Food Intake ◽  
Metabolic Regulation ◽  
Acid Metabolism ◽  
Sensing Systems ◽  
Food Intake Regulation ◽  
Glucagon Like Peptide ◽  
Glucagon Like Peptide 1 ◽  
Intake Regulation ◽  
Fatty Acid Sensing

We hypothesize that cholecystokinin (CCK) and glucagon-like peptide-1 (GLP-1) are involved in the modulation of metabolic regulation of food intake by fatty acids in fish. Therefore, we assessed in rainbow trout (Oncorhynchus mykiss) the effects of intracerebroventricular treatment with 1 ng/g of CCK-8 and with 2 ng/g of GLP-1 on food intake, expression of neuropeptides involved in food intake control and the activity of fatty acid-sensing systems in hypothalamus and hindbrain. Food intake decreased up to 24 h post-treatment to 49.8–72.3% and 3.1–17.8% for CCK-8 and GLP-1, respectively. These anorectic responses are associated with changes in fatty acid metabolism and an activation of fatty acid-sensing mechanisms in the hypothalamus and hindbrain. These changes occurred in parallel with those in the expression of anorexigenic and orexigenic peptides. Moreover, we observed that the activation of fatty acid sensing and the enhanced anorectic potential elicited by CCK-8 and GLP-1 treatments occurred in parallel with the activation of mTOR and FoxO1 and the inhibition of AMPKα, BSX and CREB. The results are discussed in the context of metabolic regulation of food intake in fish.

Effects of insulin treatment on the response to oleate and octanoate of food intake and fatty acid–sensing systems in rainbow trout

2015 ◽  
Vol 53 ◽  
pp. 124-135 ◽  
Author(s):  
M. Librán-Pérez ◽  
C. Velasco ◽  
C. Otero-Rodiño ◽  
M.A. López-Patiño ◽  
J.M. Míguez ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Rainbow Trout ◽  
Food Intake ◽  
Insulin Treatment ◽  
Sensing Systems ◽  
Fatty Acid Sensing

scholarly journals Ghrelin modulates hypothalamic fatty acid-sensing and control of food intake in rainbow trout

2015 ◽  
Vol 228 (1) ◽  
pp. 25-37 ◽  
Author(s):  
Cristina Velasco ◽  
Marta Librán-Pérez ◽  
Cristina Otero-Rodiño ◽  
Marcos A López-Patiño ◽  
Jesús M Míguez ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Rainbow Trout ◽  
Food Intake ◽  
Fatty Acid Metabolism ◽  
Metabolic Regulation ◽  
Acid Metabolism ◽  
Simultaneous Treatment ◽  
Sensing Systems ◽  
And Control ◽  
Fatty Acid Sensing

There is no information available on fish as far as the possible effects of ghrelin on hypothalamic fatty acid metabolism and the response of fatty acid-sensing systems, which are involved in the control of food intake. Therefore, we assessed in rainbow trout the response of food intake, hypothalamic fatty acid-sensing mechanisms and expression of neuropeptides involved in the control of food intake to the central treatment of ghrelin in the presence or absence of a long-chain fatty acid such as oleate. We observed that the orexigenic actions of ghrelin in rainbow trout are associated with changes in fatty acid metabolism in the hypothalamus and an inhibition of fatty acid-sensing mechanisms, which ultimately lead to changes in the expression of anorexigenic and orexigenic peptides resulting in increased orexigenic potential and food intake. Moreover, the response to increased levels of oleate of hypothalamic fatty acid-sensing systems (activation), expression of neuropeptides (enhanced anorexigenic potential) and food intake (decrease) were counteracted by the simultaneous treatment with ghrelin. These changes provide evidence for the first time in fish of a possible modulatory role of ghrelin on the metabolic regulation by fatty acid of food intake occurring in the hypothalamus.

scholarly journals Ceramides are involved in the regulation of food intake in rainbow trout (Oncorhynchus mykiss)

2016 ◽  
Vol 311 (4) ◽  
pp. R658-R668 ◽  
Author(s):  
Cristina Velasco ◽  
Marta Librán-Pérez ◽  
Cristina Otero-Rodiño ◽  
Marcos A. López-Patiño ◽  
Jesús M. Míguez ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Rainbow Trout ◽  
Food Intake ◽  
Oncorhynchus Mykiss ◽  
Signaling Pathways ◽  
Cellular Signaling ◽  
Mrna Abundance ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Sensing Systems ◽  
Fatty Acid Sensing

We hypothesize that ceramides are involved in the regulation of food intake in fish. Therefore, we assessed in rainbow trout ( Oncorhynchus mykiss) the effects of intracerebroventricular treatment with C6:0 ceramide on food intake. In a second experiment, we assessed the effects in brain areas of ceramide treatment on neuropeptide expression, fatty acid-sensing systems, and cellular signaling pathways. Ceramide treatment induced a decrease in food intake, a response opposed to the orexigenic effect described in mammals, which can be related to enhanced mRNA abundance of cocaine and amphetamine-related transcript and proopiomelanocortin and decreased mRNA abundance of Agouti-related protein and neuropeptide Y. Fatty acid-sensing systems appear to be inactivated by ceramide treatment. The mRNA abundance of integrative sensors AMPK and sirtuin 1, and the phosphorylation status of cellular signaling pathways dependent on protein kinase B, AMPK, mammalian target of rapamycin (mTOR), and forkhead box protein O1 (FoxO1) are generally activated by ceramide treatment. However, there are differences between hypothalamus and hindbrain in the phosphorylation status of AMPK (decreased in hypothalamus and increased in hindbrain), mTOR (decreased in hypothalamus and increased in hindbrain), and FoxO1 (increased in hypothalamus and decreased in hindbrain) to ceramide treatment. The results suggest that ceramides are involved in the regulation of food intake in rainbow trout through mechanisms comparable to those characterized previously in mammals in some cases.

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