Bacillus amyloliquefaciens BLCC1-0238 Can Effectively Improve Laying Performance and Egg Quality Via Enhancing Immunity and Regulating Reproductive Hormones of Laying Hens

2019 ◽  
Vol 12 (1) ◽  
pp. 246-252 ◽  
Author(s):  
Yufa Zhou ◽  
Song Li ◽  
Quanhai Pang ◽  
Zengmin Miao
Keyword(s):  
Bacillus Amyloliquefaciens ◽  
Laying Hens ◽  
Egg Quality ◽  
Reproductive Hormones ◽  
Laying Performance

scholarly journals Cuticle Secretion Duration in the Uterus Is One of the Major Factors Affecting Eggshell Cuticle Deposition in White Leghorn Laying Hens

2020 ◽  
Author(s):  
Xia CHEN ◽  
Zhaoxiang HE ◽  
Xingzheng LI ◽  
Jianlou SONG ◽  
Mingyi HUANG ◽  
...  
Keyword(s):  
Laying Hens ◽  
Egg Quality ◽  
Activity Rhythm ◽  
Expression Patterns ◽  
Genetic Regulation ◽  
Reproductive Hormones ◽  
Poultry Production ◽  
Factors Affecting ◽  
Laying Performance ◽  
Major Factors

Abstract Background: The cuticle formed in the uterus is the outermost layer as the first defense line of eggshell against microbial invasions in most avian species, and analyzing its genetic regulation and influencing factors are of great importance to egg biosecurity in poultry production. The current study compared the uterine transcriptome and proteome of laying hens producing eggs with good and poor cuticle deposition (GC and PC, the top and tail of the cuticle deposition distribution), and identified several genes involved with eggshell cuticle deposition.Results: Overall, transcriptomic analysis identified 53 differentially expressed genes (DEGs) between PC vs GC group hens, among which 25 were up-regulated and 28 were down-regulated. No differences were found in the uterine proteome. Several up-regulated DEGs of PC vs GC hen uterus, including PTGDS, PLCG2, ADM and PRLR related to uterine functions and reproductive hormones, were validated by qPCR analysis. Phenotypic measurements for verification between GC and PC hens showed GC hens had longer laying interval between two consecutive ovipositions (25.64 ± 1.23 vs 24.94 ± 1.12 h), thicker eggshell thickness (352.01 ± 23.04 vs 316.20 ± 30.58 μm) and more laying days (20.67 ± 2.58 vs 18.48 ± 2.46) within 27 laying days (P < 0.05). Apart from eggshell traits, other egg quality traits didn’t differ. The results above suggested that the expression patterns of PTGDS, PLCG2, ADM and PRLR genes in the uterus regulated the muscular activity rhythm and secretion function, which ensured the eggshell and cuticle formation duration, and lead to the good eggshell and cuticle quality of the GC hens.Conclusions: The present study demonstrated eggshell and cuticle secretion duration in the uterus is one of the major factors affecting eggshell cuticle deposition in laying hens. PTGDS, PLCG2, ADM and PRLR genes were discovered and might play crucial roles in cuticle deposition by regulating the uterine muscular activities and secretion function. Moreover, the cuticle is associated with the eggshell quality and laying performance, implying the importance of selection to the cuticle trait in laying hens. The findings in present study provide new insights into the genetic regulation of cuticle deposition in laying hens and establish a foundation for further investigations.

scholarly journals Effects of dietary protein, energy and β-mannanase on laying performance, egg quality, and ileal amino acid digestibility in laying hens.

2021 ◽  
pp. 101312
Author(s):  
Dima White ◽  
Roshan Adhikari ◽  
Jinquan Wang ◽  
Chongxiao Chen ◽  
Jae Hwan Lee ◽  
...  
Keyword(s):  
Amino Acid ◽  
Dietary Protein ◽  
Laying Hens ◽  
Egg Quality ◽  
Protein Energy ◽  
Laying Performance

Influence of peeling and inorganic calcium concentration on the utilisation of giant taro (Alocasia macrorrhiza) corm meal by laying hens

2019 ◽  
Vol 59 (8) ◽  
pp. 1553 ◽  
Author(s):  
S. S. Diarra ◽  
I. Wamekeni ◽  
A. Vunagilee ◽  
H. Lavaka ◽  
T. Finau
Keyword(s):  
Egg Production ◽  
Laying Hens ◽  
Egg Quality ◽  
Shape Index ◽  
Egg Mass ◽  
Feed Conversion ◽  
Shell Quality ◽  
Laying Performance ◽  
Calcium Source ◽  
Main Effects

The effects of peeling and limestone flour level on the utilisation of Alocasia macrorrhiza corm meal (ACM) by laying hens were investigated. Maize-based diets with two concentrations of limestone flour (40 and 60 g/kg) and diets containing 200 g peeled and unpeeled ACM meal/kg with the same levels of limestone flour were fed to five replicate pens containing 10 20-week-old Shaver brown pullets (1635 ± 148 g), each for 105 days. Results showed no interaction or main effects of ACM or limestone concentration on feed intake, egg weight, egg mass, feed conversion efficiency, egg shape index, surface area and Haugh unit (P &gt; 0.05). Feeding whole ACM with 40 g limestone/kg diet reduced hen-day production and shell thickness (P &lt; 0.05) but this was overcome by increasing limestone concentration to 60 g/kg diet. Peeled ACM with both concentrations of limestone flour maintained laying performance and egg quality. We concluded that feeding 200 g whole ACM depresses egg production and shell quality in laying hens, but increasing dietary limestone concentration overcomes this adverse effect. The concentration of limestone flour in the diet has no effect on laying performance. Higher inclusion levels of ACM, calcium source and level that will maintain laying performance and reduce feed cost need to be investigated.

scholarly journals Effects of Ginger Extract on Laying Performance, Egg Quality, and Antioxidant Status of Laying Hens

Animals ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 857 ◽  
Author(s):  
Wen ◽  
Gu ◽  
Tao ◽  
Cheng ◽  
Wang ◽  
...  
Keyword(s):  
Antioxidant Status ◽  
Laying Hens ◽  
Egg Quality ◽  
Basal Diet ◽  
Cholesterol Content ◽  
Ginger Extract ◽  
Sod Activity ◽  
Egg Weight ◽  
Laying Performance ◽  
Yolk Cholesterol

The objective of this study was to investigate the effects of ginger extract (GE) as a dietary supplement for laying hens. A total of 40-week-old 288 Hyline Brown laying hens were randomly divided into two groups with six replicates, and fed a basal diet with or without 100 g/t GE for eight weeks. Dietary GE supplementation increased egg weight, albumin height, and Haugh unit of eggs, and decreased yolk cholesterol content and activities of alanine transaminase and aspartate transaminase in serum at eight weeks. Moreover, GE resulted in higher total superoxide dismutase (T-SOD) activity and lower malondialdehyde (MDA) content in yolk at four and eight weeks and in serum. It was concluded that GE was effective in increasing egg weight and improving the egg quality and antioxidant status of laying hens.

scholarly journals Dietary cadmium chloride impairs shell biomineralization by disrupting the metabolism of the eggshell gland in laying hens

2020 ◽  
Vol 98 (2) ◽  
Author(s):  
Mingkun Zhu ◽  
Huaiyu Li ◽  
Liping Miao ◽  
Lanlan Li ◽  
Xinyang Dong ◽  
...  
Keyword(s):  
Laying Hens ◽  
Egg Quality ◽  
Dietary Supplementation ◽  
Basal Diet ◽  
Complement C3 ◽  
Mrna Levels ◽  
Palisade Layer ◽  
Positive Effects ◽  
Laying Performance ◽  
Dietary Cadmium

Abstract In this study, we identified cadmium (Cd) as a potential endocrine disruptor that impairs laying performance, egg quality, and eggshell deposition and induces oxidative stress and inflammation in the eggshell glands of laying hens. A total of 480 38-wk-old laying hens were randomly assigned into 5 groups that were fed a basal diet (control) or a basal diet supplemented with Cd (provided as CdCl2·2.5 H2O) at 7.5, 15, 30, and 60 mg Cd per kg feed for 9 wk. The results showed that, when compared with the control group, a low dose of dietary Cd (7.5 mg/kg) had positive effects on egg quality by improving albumen height, Haugh unit, yolk color, and shell thickness at the third or ninth week. However, with the increase in the dose and duration of Cd exposure, the laying performance, egg quality, and activities of eggshell gland antioxidant enzymes (catalase [CAT], glutathione peroxide [GSH-Px]), and ATPase (Na+/K+-ATPase, Ca2+-ATPase, and Mg2+-ATPase) deteriorated, and the activity of total nitric oxide synthase (T-NOS) and the level of malondialdehyde (MDA) increased significantly (P &lt; 0.05). The histopathology and real-time quantitative PCR results showed that Cd induced endometrial epithelial cell proliferation accompanied by upregulation of the mRNA levels of progesterone receptor (PgR) and epidermal growth factor receptor (EGFR), downregulation of the mRNA levels of estrogen receptor α (ERα) and interleukin 6 (IL6), and inflammation of the eggshell gland accompanied by significantly increased expression of complement C3 and pro-inflammatory cytokine tumor necrosis factor α (TNFα) (P &lt; 0.05). In addition, the ultrastructure of the eggshell showed that dietary supplementation with 7.5 mg/kg Cd increased the palisade layer and total thickness of the shell, but with the increase in dietary Cd supplementation (30 and 60 mg/kg) the thickness of the palisade layer and mammillary layer decreased significantly (P &lt; 0.05), and the outer surface of the eggshell became rougher. Correspondingly, the expression of calbindin 1 (CALB1), ovocalyxin-32 (OCX-32), ovocalyxin-36 (OCX-36), osteopontin (SPP1), and ovocledidin-17 (OC-17) decreased significantly (P &lt; 0.05) with increasing dietary Cd supplementation. Conclusively, the present study demonstrates that dietary supplementation with Cd negatively affects laying performance, egg quality, and eggshell deposition by disturbing the metabolism of eggshell glands in laying hens but has a positive effect on egg quality at low doses.

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