Physicochemical and Biological Properties of Eel By-Products Protein Hydrolysates: Potential Application to Meat Product Preservation

In the medical device sector, bloom index and residual endotoxins should be controlled, as they are crucial regulators of the device’s physicochemical and biological properties. It is also imperative to identify a suitable crosslinking method to increase mechanical integrity, without jeopardising cellular functions of gelatin-based devices. Herein, gelatin preparations with variable bloom index and endotoxin levels were used to fabricate non-crosslinked and polyethylene glycol succinimidyl glutarate crosslinked gelatin scaffolds, the physicochemical and biological properties of which were subsequently assessed. Gelatin preparations with low bloom index resulted in hydrogels with significantly (p < 0.05) lower compression stress, elastic modulus and resistance to enzymatic degradation, and significantly higher (p < 0.05) free amine content than gelatin preparations with high bloom index. Gelatin preparations with high endotoxin levels resulted in films that induced significantly (p < 0.05) higher macrophage clusters than gelatin preparations with low endotoxin level. Our data suggest that the bloom index modulates the physicochemical properties, and the endotoxin content regulates the biological response of gelatin biomaterials. Although polyethylene glycol succinimidyl glutarate crosslinking significantly (p < 0.05) increased compression stress, elastic modulus and resistance to enzymatic degradation, and significantly (p < 0.05) decreased free amine content, at the concentration used, it did not provide sufficient structural integrity to support cell culture. Therefore, the quest for the optimal gelatin crosslinker continues.

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Anthocyanins Recovered from Agri-Food By-Products Using Innovative Processes: Trends, Challenges, and Perspectives for Their Application in Food Systems

Molecules ◽

10.3390/molecules26092632 ◽

2021 ◽

Vol 26 (9) ◽

pp. 2632

Author(s):

Henrique Silvano Arruda ◽

Eric Keven Silva ◽

Nayara Macêdo Peixoto Araujo ◽

Gustavo Araujo Pereira ◽

Glaucia Maria Pastore ◽

...

Keyword(s):

Food Systems ◽

Food Industry ◽

Biological Properties ◽

Extraction Techniques ◽

Naturally Occurring ◽

Current Review ◽

Natural Colorants ◽

Conventional Extraction ◽

The Stability ◽

By Products

Anthocyanins are naturally occurring phytochemicals that have attracted growing interest from consumers and the food industry due to their multiple biological properties and technological applications. Nevertheless, conventional extraction techniques based on thermal technologies can compromise both the recovery and stability of anthocyanins, reducing their global yield and/or limiting their application in food systems. The current review provides an overview of the main innovative processes (e.g., pulsed electric field, microwave, and ultrasound) used to recover anthocyanins from agri-food waste/by-products and the mechanisms involved in anthocyanin extraction and their impacts on the stability of these compounds. Moreover, trends and perspectives of anthocyanins’ applications in food systems, such as antioxidants, natural colorants, preservatives, and active and smart packaging components, are addressed. Challenges behind anthocyanin implementation in food systems are displayed and potential solutions to overcome these drawbacks are proposed.

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The effect of Ag substitution on physicochemical and biological properties of sol-gel derived 60%SiO2–31%CaO–4%P2O5–5%Li2O (mol%) quaternary bioactive glass

Ceramics International ◽

10.1016/j.ceramint.2021.02.173 ◽

2021 ◽

Author(s):

Mohsen Rahmani ◽

Amirhossein Moghanian ◽

Morteza Saghafi Yazdi

Keyword(s):

Bioactive Glass ◽

Sol Gel ◽

Biological Properties ◽

Physicochemical And Biological Properties ◽

Ag Substitution

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Characterization of Protein Hydrolysates from Fish Discards and By-Products from the North-West Spain Fishing Fleet as Potential Sources of Bioactive Peptides

Marine Drugs ◽

10.3390/md19060338 ◽

2021 ◽

Vol 19 (6) ◽

pp. 338

Author(s):

Andreia Henriques ◽

José A. Vázquez ◽

Jesus Valcarcel ◽

Rogério Mendes ◽

Narcisa M. Bandarra ◽

...

Keyword(s):

Inhibitory Activity ◽

Bioactive Peptides ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Protein Hydrolysates ◽

Scavenging Activity ◽

North West ◽

Chelating Activity ◽

The North ◽

By Products

Fish discards and by-products can be transformed into high value-added products such as fish protein hydrolysates (FPH) containing bioactive peptides. Protein hydrolysates were prepared from different parts (whole fish, skin and head) of several discarded species of the North-West Spain fishing fleet using Alcalase. All hydrolysates had moisture and ash contents lower than 10% and 15%, respectively. The fat content of FPH varied between 1.5% and 9.4% and had high protein content (69.8–76.6%). The amino acids profiles of FPH are quite similar and the most abundant amino acids were glutamic and aspartic acids. All FPH exhibited antioxidant activity and those obtained from Atlantic horse mackerel heads presented the highest 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, reducing power and Cu2+ chelating activity. On the other hand, hydrolysates from gurnard heads showed the highest ABTS radical scavenging activity and Fe2+ chelating activity. In what concerns the α-amylase inhibitory activity, the IC50 values recorded for FPH ranged between 5.70 and 84.37 mg/mL for blue whiting heads and whole Atlantic horse mackerel, respectively. α-Glucosidase inhibitory activity of FPH was relatively low but all FPH had high Angiotensin Converting Enzyme (ACE) inhibitory activity. Considering the biological activities, these FPH are potential natural additives for functional foods or nutraceuticals.

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