scholarly journals Use of Non-Conventional Yeast Yarrowia lipolytica in Treatment or Upgradation of Hydrophobic Industry Wastes

2021 ◽  
Author(s):  
Katarzyna Wierzchowska ◽  
Bartłomiej Zieniuk ◽  
Agata Fabiszewska
Keyword(s):  
Yarrowia Lipolytica ◽  
Development Policy ◽  
Food Industry ◽  
Yeast Species ◽  
Current Knowledge ◽  
Waste Cooking Oil ◽  
Application Potential ◽  
Palm Oil Mill ◽  
Yeast Yarrowia Lipolytica ◽  
Olive Mill

AbstractThe review aims to summarize the current knowledge on the possibility of using non-conventional yeast species Yarrowia lipolytica in the treatment and upgradation of industry wastes. Importantly Y. lipolytica yeast is argued as generally recognized as safe species, what indicates the high application potential of the reviewed technologies. Special emphasis in the paper was given on microbial processing of the food industry wastes, including fish and animals’ wastes utilization. Yarrowia-based processing of waste cooking oil or oil-bearing plants wastewaters, such as palm oil mill effluents or olive mill wastewater was reviewed. Recent advances in biosynthesis of valuable metabolites (e.g. lipases or microbial oil) with simultaneous wastes utilization by Y. lipolytica are additionally discussed. The broad implications of the present paper are a part of sustainable development policy. Graphic Abstract

scholarly journals Bioproducts generation from carboxylate platforms by the non-conventional yeast Yarrowia lipolytica

2021 ◽  
Author(s):  
Young-Kyoung Park ◽  
Cristina González-Fernández ◽  
Raúl Robles-Iglesias ◽  
Lea Vidal ◽  
Pierre Fontanille ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Yarrowia Lipolytica ◽  
Volatile Fatty Acids ◽  
Oleaginous Yeast ◽  
Current Knowledge ◽  
Value Added ◽  
Building Blocks ◽  
Renewable Sources ◽  
The Relationship ◽  
Yeast Yarrowia Lipolytica

Abstract In recent years, there has been a growing interest in the use of renewable sources for bio-based production aiming at developing sustainable and feasible approaches towards a circular economy. Among these renewable sources, organic wastes (OWs) can be anaerobically digested to generate carboxylates like volatile fatty acids (VFAs), lactic acid, and longer-chain fatty acids that are regarded as novel building blocks for the synthesis of value-added compounds by yeasts. This review discusses on the processes that can be used to create valuable molecules from OW-derived VFAs; the pathways employed by the oleaginous yeast Yarrowia lipolytica to directly metabolize such molecules; and the relationship between OW composition, anaerobic digestion, and VFA profiles. The review also summarizes the current knowledge about VFA toxicity, the pathways by which VFAs are metabolized, and the metabolic engineering strategies that can be employed in Y. lipolytica to produce value-added biobased compounds from VFAs.

scholarly journals Phylogenomic and biochemical analysis reassesses temperate marine yeast Yarrowia lipolytica NCIM 3590 to be Yarrowia bubula

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Prashant Gaikwad ◽  
Swanand Joshi ◽  
Akshay Mandlecha ◽  
Ameeta RaviKumar
Keyword(s):  
Yarrowia Lipolytica ◽  
Biochemical Analysis ◽  
Reference Strain ◽  
Yeast Species ◽  
Lipolytic Activity ◽  
Extracellular Lipase ◽  
Marine Yeast ◽  
Molecular Phylogenetic ◽  
Yeast Yarrowia Lipolytica ◽  
Tblastn Analysis

AbstractYarrowia clade contains yeast species morphologically, ecologically, physiologically and genetically diverse in nature. Yarrowia lipolytica NCIM 3590 (NCIM 3590), a biotechnologically important strain, isolated from Scottish sea waters was reinvestigated for its phenotypic, biochemical, molecular and genomic properties as it exhibited characteristics unlike Y. lipolytica, namely, absence of extracellular lipolytic activity, growth at lower temperatures (less than 20 °C) and in high salt concentrations (10% NaCl). Molecular identification using ITS and D1/D2 sequences suggested NCIM 3590 to be 100% identical with reference strain Yarrowia bubula CBS 12934 rather than Y. lipolytica CBS 6124 (87% identity) while phylogenetic analysis revealed that it clustered with Y. bubula under a separate clade. Further, whole genome sequencing of NCIM 3590 was performed using Illumina NextSeq technology and the draft reported here. The overall genome relatedness values obtained by dDDH (94.1%), ANIb/ANIm (99.41/99.42%) and OrthoANI (99.47%) indicated proximity between NCIM 3590 and CBS 12934 as compared to the reference strain Y. lipolytica. No extracellular lipase activity could be detected in NCIM 3590 while LIP2 gene TBLASTN analysis suggests a low 42% identity with e value 2 e−77 and 62% coverage. Hence molecular, phylogenetic, genomics, biochemical and microbial analyses suggests it belongs to Yarrowia bubula.

Ochratoxin A is degraded by Yarrowia lipolytica and generates non-toxic degradation products

2016 ◽  
Vol 9 (2) ◽  
pp. 269-278 ◽  
Author(s):  
Q. Yang ◽  
J. Wang ◽  
H. Zhang ◽  
C. Li ◽  
X. Zhang
Keyword(s):  
Ochratoxin A ◽  
Yarrowia Lipolytica ◽  
Food Industry ◽  
Initial Level ◽  
Degradation Products ◽  
Negative Effect ◽  
Yeast Concentration ◽  
Natural Decay ◽  
Yeast Yarrowia Lipolytica

The mycotoxin ochratoxin A (OTA) is a common contaminant of various plant-derived foods and feeds. However, methods for complete decontamination remain to be established. Recently, biological approaches for mycotoxin removal using various species of yeast have been explored. In the present study, we investigated the efficacy of OTA degradation by the yeast Yarrowia lipolytica under various conditions, altering yeast concentration, temperature, pH, and concentration of OTA in order to determine the optimal requirements of this species. At a yeast concentration of 108 cells/ml, the degradation rate was higher than that observed at any other concentration and, after 24 h, the OTA concentration was reduced to almost half of the initial level introduced to the culture. Further, Y. lipolytica cultured at 28 °C showed the highest level of OTA degradation. Similarly, the culture performed optimally at a pH of 4. The initial concentration of OTA also affected the ability of the yeast to degrade OTA, with the level of degradation being the highest when the initial OTA concentration was 0.1 μg/ml. Moreover, we also tested the toxicity of the OTA biodegradation products using HepG2 cells to determine the physiological applicability of this yeast species in the food industry and observed that these products were notably less toxic than non-degraded OTA. Y. lipolytica effectively reduced natural decay incidence of grapes, and had no negative effect to the storage quality of grape fruits. Taken together, these data suggest that Y. lipolytica could be a viable OTA contamination prevention/treatment option and additional research concerning its commercial use is warranted.

scholarly journals Psychrotrophic yeast Yarrowia lipolytica NCYC 789 mediates the synthesis of antimicrobial silver nanoparticles via cell-associated melanin

AMB Express ◽  
2013 ◽  
Vol 3 (1) ◽  
pp. 32 ◽  
Author(s):  
Mugdha Apte ◽  
Devashree Sambre ◽  
Shital Gaikawad ◽  
Swanand Joshi ◽  
Ashok Bankar ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Yarrowia Lipolytica ◽  
Yeast Yarrowia Lipolytica

scholarly journals Stable isotope and chemical inhibition analyses suggested the existence of a non-mevalonate-like pathway in the yeast Yarrowia lipolytica

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Sivamoke Dissook ◽  
Tomohisa Kuzuyama ◽  
Yuri Nishimoto ◽  
Shigeru Kitani ◽  
Sastia Putri ◽  
...  
Keyword(s):  
Yarrowia Lipolytica ◽  
Oleaginous Yeast ◽  
Isoprenoid Biosynthesis ◽  
Mep Pathway ◽  
Authentic Standard ◽  
Chemical Inhibition ◽  
Chromatographic Peaks ◽  
Methyl Erythritol Phosphate ◽  
Limiting Condition ◽  
Yeast Yarrowia Lipolytica

AbstractMethyl erythritol phosphate (MEP) is the metabolite found in the MEP pathway for isoprenoid biosynthesis, which is known to be utilized by plants, algae, and bacteria. In this study, an unprecedented observation was found in the oleaginous yeast Yarrowia lipolytica, in which one of the chromatographic peaks was annotated as MEP when cultivated in the nitrogen limiting condition. This finding raised an interesting hypothesis of whether Y. lipolytica utilizes the MEP pathway for isoprenoid biosynthesis or not, because there is no report of yeast harboring the MEP pathway. Three independent approaches were used to investigate the existence of the MEP pathway in Y. lipolytica; the spiking of the authentic standard, the MEP pathway inhibitor, and the 13C labeling incorporation analysis. The study suggested that the mevalonate and MEP pathways co-exist in Y. lipolytica and the nitrogen limiting condition triggers the utilization of the MEP pathway in Y. lipolytica.

scholarly journals Droplet-based microfluidic high-throughput screening of heterologous enzymes secreted by the yeast Yarrowia lipolytica

2017 ◽  
Vol 16 (1) ◽  
Author(s):  
Thomas Beneyton ◽  
Stéphane Thomas ◽  
Andrew D. Griffiths ◽  
Jean-Marc Nicaud ◽  
Antoine Drevelle ◽  
...  
Keyword(s):  
Yarrowia Lipolytica ◽  
High Throughput ◽  
High Throughput Screening ◽  
Yeast Yarrowia Lipolytica

Cañihua (Chenopodium pallidicaule Aellen) a promising superfood in food industry: a review

2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Jerry Fredy Gomez Cahuata ◽  
Yessica Estefany Rosas-Quina ◽  
Erika Pachari Vera
Keyword(s):  
Food Industry ◽  
Unsaturated Fatty Acids ◽  
Protein Quality ◽  
Current Knowledge ◽  
Scientific Information ◽  
Essential Amino Acids ◽  
High Concentration ◽  
Content Type ◽  
Functional Value ◽  
Potential Applications

Purpose The purpose of this paper is to divulge the current knowledge about the nutritional and functional characteristics of Cañihua (Chenopodium pallidicaule Aellen), in addition to its potential applications in the food industry since research studies related to it are still limited compared to other cereals of greater diffusion. Design/methodology/approach The scientific information was collected from Web of Science, Scopus and Google Scholar databases, using keywords such as nutrition value of Chenopodium pallidicaule, amaranth and pseudocereals. Consistent information was selected according to its relevance, year of publication and accuracy with the topic. A total of 49 research papers were selected. Findings Cañihua is a grain with high nutritional potential, considered a superfood because it has a high protein quality, a balanced composition of essential amino acids and unsaturated fatty acids, with a high concentration of linoleic and oleic acid. Besides, it has a good level of bioactive compounds with high antioxidant capacity. However, its production and consumption are limited outside its area of origin, although its cultivation is possible under harsh conditions. Originality/value This paper, through a systematic bibliographic review, highlights the potential of cañihua to be considered in the development of food products with high nutritional and functional value. The information compiled will help researchers and professionals become aware of the importance of this grain and join forces in its processing and enhancement of its attributes.

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