Quantitative Cytotoxicity, Cellular Uptake and Radioprotection Effect of Cerium Oxide Nanoparticles in MRC-5 Normal Cells and MCF-7 Cancerous Cells

Background: Drug resistance due to genetic variations renders many therapeutic methods such as surgery, radiotherapy, chemotherapy, and hormone therapy unsuccessful in eradicating cancerous cells. Nowadays, application of nanoparticles (NPs) has been promising in destroying cancerous cells without side effects on normal cells. Objectives: This study aimed to investigate the antioxidant and anticancer effects of biosynthesized cerium oxide nanoparticles (CeO2 -NPs) on a hepatic carcinoma cell line. Methods: MTT assay was used to determine the cytotoxicity of CeO2 -NPs in concentrations of 0, 15.6, 31.2, 62.5, 125, and 250 μg/mL after 24, 48, and 72 hours of incubation. Moreover, the expression levels of catalase (CAT) and superoxide dismutase (SOD) (the antioxidant genes) were investigated at different concentrations of CeO2 -NPs using real-time polymerase chain reaction (PCR). Results: Our results showed a significant toxicity of the synthesized NPs against the cancerous liver cells. The IC50 calculated for CeO2 -NPs was 500 μg/mL at 24 hours of incubation. In addition, the expression levels of CAT and SOD significantly (P<0.05) increased upon the treatment of cells with CeO2 -NPs (500 µg /mL) compared to the untreated cells. Conclusion: Considering the minimal effects of the biosynthesized CeO2 -NPs on normal cells and on the other hand their considerable toxicity against hepatic cancer cells, these NPs could be utilized in medicine and in the development of new drugs for cancer cells.

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Cerium oxide nanoparticles at the nano-bio interface: size-dependent cellular uptake

Artificial Cells Nanomedicine and Biotechnology ◽

10.1080/21691401.2018.1521818 ◽

2018 ◽

Vol 46 (sup3) ◽

pp. S956-S963 ◽

Cited By ~ 13

Author(s):

Sushant Singh ◽

Anh Ly ◽

Soumen Das ◽

Tamil S. Sakthivel ◽

Swetha Barkam ◽

...

Keyword(s):

Cerium Oxide ◽

Cellular Uptake ◽

Cerium Oxide Nanoparticles ◽

Oxide Nanoparticles ◽

Size Dependent

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Protein adsorption and cellular uptake of cerium oxide nanoparticles as a function of zeta potential

Biomaterials ◽

10.1016/j.biomaterials.2007.07.029 ◽

2007 ◽

Vol 28 (31) ◽

pp. 4600-4607 ◽

Cited By ~ 603

Author(s):

Swanand Patil ◽

Amanda Sandberg ◽

Eric Heckert ◽

William Self ◽

Sudipta Seal

Keyword(s):

Zeta Potential ◽

Protein Adsorption ◽

Cerium Oxide ◽

Cellular Uptake ◽

Cerium Oxide Nanoparticles ◽

Oxide Nanoparticles

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Cellular uptake and activity of heparin functionalised cerium oxide nanoparticles in monocytes

Biomaterials ◽

10.1016/j.biomaterials.2013.02.042 ◽

2013 ◽

Vol 34 (17) ◽

pp. 4377-4386 ◽

Cited By ~ 37

Author(s):

S.R. Simon Ting ◽

John M. Whitelock ◽

Romana Tomic ◽

Cindy Gunawan ◽

Wey Yang Teoh ◽

...

Keyword(s):

Cerium Oxide ◽

Cellular Uptake ◽

Cerium Oxide Nanoparticles ◽

Oxide Nanoparticles

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Cellular uptake and reactive oxygen species modulation of cerium oxide nanoparticles in human monocyte cell line U937

Biomaterials ◽

10.1016/j.biomaterials.2012.07.024 ◽

2012 ◽

Vol 33 (31) ◽

pp. 7915-7924 ◽

Cited By ~ 73

Author(s):

Megan S. Lord ◽

MoonSun Jung ◽

Wey Yang Teoh ◽

Cindy Gunawan ◽

James A. Vassie ◽

...

Keyword(s):

Reactive Oxygen Species ◽

Cell Line ◽

Cerium Oxide ◽

Cellular Uptake ◽

Human Monocyte ◽

Cerium Oxide Nanoparticles ◽

Oxide Nanoparticles ◽

Oxygen Species ◽

Human Monocyte Cell Line ◽

Monocyte Cell

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Polyacrylic Acid Modified Cerium Oxide Nanoparticles: Synthesis and Characterization as a Peroxidase Mimic for Non-Enzymatic H2O2 Sensor

Current Nanoscience ◽

10.2174/1573413715666191204124329 ◽

2020 ◽

Vol 16 (5) ◽

pp. 816-828

Author(s):

Gurdeep Rattu ◽

Nishtha Khansili ◽

Prayaga M. Krishna

Keyword(s):

Hydrogen Peroxide ◽

Cerium Oxide ◽

Polyacrylic Acid ◽

Spectroscopic Analysis ◽

Cerium Oxide Nanoparticles ◽

Oxide Nanoparticles ◽

Peroxidase Mimic ◽

Ft Ir ◽

H2o2 Sensing ◽

Fluorescence Spectrometer

Background: Cerium oxide nanoparticles (nanoceria) are efficient free-radical scavengers due to their dual valence state and thus exhibit optical and catalytic properties. Therefore, the main purpose of this work was to understand the peroxidase mimic activity of polymer-stabilized nanoceria for enzyme-less H2O2 sensing by fluorescence spectrometer. Objective: This research revealed the development of fluorescence hydrogen peroxide nanosensor based on the peroxidase-like activity of polyacrylic acid stabilized nanoceria (PAA-CeO2 Nps). Methods: PAA-CeO2 Nps were synthesized by simple cross-linking reaction at a low temperature and characterized by XRD, SEM, Zeta potential, TGA, FT-IR and UV-VIS spectroscopic analysis. H2O2 sensing was performed by a fluorescence spectrometer. Results:: The synthesized polymer nanocomposite was characterized by XRD, SEM, TGA, FT-IR and UV-VIS spectroscopic analysis. The XRD diffraction patterns confirmed the polycrystalline nature and SEM micrograph showed nanoparticles having hexagonal symmetry and crystallite size of 32 nm. The broad peak of Ce–O bond appeared at 508 cm-1. UV-VIS measurements revealed a welldefined absorbance peak around 315 nm and an optical band-gap of 3.17 eV. As synthesized PAACeO2 Nps effectively catalysed the decomposition of hydrogen peroxide (H2O2) into hydroxyl radicals. Then terephthalic acid was oxidized by hydroxyl radical to form a highly fluorescent product. Under optimized conditions, the linear range for determination of hydrogen peroxide was 0.01 - 0.2 mM with a limit of detection (LOD) of 1.2 μM. Conclusion: The proposed method is ideally suited for the sensing of H2O2 at a low cost and this detection system enabled the sensing of analytes (sugars), which can enzymatically generate hydrogen peroxide.

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