Application of Mesenchymal Stem Cells Derived Artificial Microvesicles for the Treatment of Canine Skin Wound

2021 ◽  
Author(s):  
Elena Zakirova ◽  
Anastasiya Valeeva ◽  
Svetlana Sofronova ◽  
Maksim Tambovsky ◽  
Catrin Rutland ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Skin Wound

Abstract 5: Neovascularization By Substance-p- Mobilized Epc And Msc In Vitro And In Vivo

2014 ◽  
Vol 115 (suppl_1) ◽  
Author(s):  
Hyun Sook Hong ◽  
Suna Kim ◽  
Youngsook Son
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Substance P ◽  
Network Formation ◽  
Skin Wound ◽  
Hematopoietic Stem ◽  
Vessel Structure

Bone marrow stem cells, especially, endothelial precursor cells (EPC), mesenchymal stem cells (MSC) or hematopoietic stem cell (HSC) are expected as reparative cells for the repair of a variety of tissue damages such as stroke and myocardial infarction, even though their role in the repair is not demonstrated. This report was investigated to find a role of Substance-p (SP) as a reparative agent in the tissue repair requiring EPC and MSC. In order to examine EPC (EPC SP ) and MSC (MSC SP ) mobilized by SP, we injected SP intravenously for consecutive 2 days and saline was injected as a vehicle. At 3 post injection, peripheral blood (PB) was collected.To get mesenchymal stem cells or endothelial progenitor cells, MNCs were incubated in MSCGM or EGM-2 respectively for 10 days. Functional characteristics of the EPC SP were proven by the capacity to form endothelial tubule network in the matrigel in vitro and in the matrigel plug assay in vivo. In contrast, MSC SP did not form a tube-like structure but formed a pellet-structure on matrigel. However, when both cells were premixed before the matrigel assay, much longer and branched tubular network was formed, in which a-SMA expressing MSC SP were decorating outside of the endothelial tube, especially enriched at the bifurcating point. MSC SP may contribute and reinforce elaborate vascular network formation in vivo by working as pericyte-like cells. Thus, the EPC SP and MSC SP were labeled with PKH green and PKH red respectively and their tubular network was examined. Well organized tubular network was formed, which was covered by PKH green labeled cells and was decorated in a punctate pattern by PKH red labeled cells. In order to investigate the role of EPC SP and MSC SP specifically in vivo, rabbit EPC SP and MSC SP were transplanted to full thickness skin wound. The vessel of EPC SP -transplanted groups was UEA-lectin+, which was not covered with a-SMA+ pericytes but EPC SP + MSC SP -transplanted groups showed, in part, a-SMA+ pericyte-encircled UEA-lectin+ vessels. This proved the specific role of MSC SP as pericytes. From these data, we have postulated that the collaboration of MSC and EPC is essential for normal vessel structure and furthermore, accelerated wound healing as ischemia diseases, which can be stimulated through by SP injection.

scholarly journals The role of placenta-derived mesenchymal stem cells in healing of induced full-thickness skin wound in a mouse model

IUBMB Life ◽  
2015 ◽  
Vol 67 (9) ◽  
pp. 701-709 ◽  
Author(s):  
Somia H. Abd-Allah ◽  
Amal S. El-Shal ◽  
Sally M. Shalaby ◽  
Eman Abd-Elbary ◽  
Nehad F. Mazen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Mouse Model ◽  
Skin Wound ◽  
Full Thickness ◽  
Full Thickness Skin ◽  
Thickness Skin

scholarly journals Mesenchymal stem cells in skin wound healing

2016 ◽  
Vol 10 (3) ◽  
pp. 228-234 ◽  
Author(s):  
Işıl Aydemir ◽  
Şamil Öztürk ◽  
Pınar Kılıçaslan Sönmez ◽  
Mehmet İbrahim Tuğlu
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Mesenchymal Stem Cells ◽  
Skin Wound ◽  
Skin Wound Healing

scholarly journals Apoptotic bodies derived from mesenchymal stem cells promote cutaneous wound healing via regulating the functions of macrophages

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Jin Liu ◽  
Xinyu Qiu ◽  
Yajie Lv ◽  
Chenxi Zheng ◽  
Yan Dong ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Mesenchymal Stem Cells ◽  
Mouse Skin ◽  
Skin Wound ◽  
Therapeutic Effects ◽  
Cutaneous Wound Healing ◽  
Apoptotic Bodies ◽  
Skin Wound Healing ◽  
Cutaneous Wound

Abstract Background As the major interface between the body and the external environment, the skin is liable to various injuries. Skin injuries often lead to severe disability, and the exploration of promising therapeutic strategies is of great importance. Exogenous mesenchymal stem cell (MSC)-based therapy is a potential strategy due to the apparent therapeutic effects, while the underlying mechanism is still elusive. Interestingly, we observed the extensive apoptosis of exogenous bone marrow mesenchymal stem cells (BMMSCs) in a short time after transplantation in mouse skin wound healing models. Considering the roles of extracellular vesicles (EVs) in intercellular communication, we hypothesized that the numerous apoptotic bodies (ABs) released during apoptosis may partially contribute to the therapeutic effects. Methods ABs derived from MSCs were extracted, characterized, and applied in mouse skin wound healing models, and the therapeutic effects were evaluated. Then, the target cells of ABs were explored, and the effects of ABs on macrophages were investigated in vitro. Results We found ABs derived from MSCs promoted cutaneous wound healing via triggering the polarization of macrophages towards M2 phenotype. In addition, the functional converted macrophages further enhanced the migration and proliferation abilities of fibroblasts, which together facilitated the wound healing process. Conclusions Collectively, our study demonstrated that transplanted MSCs promoted cutaneous wound healing partially through releasing apoptotic bodies which could convert the macrophages towards an anti-inflammatory phenotype that plays a crucial role in the tissue repair process.

Studies on the Efficacy of Single and Twice Application of Mesenchymal Stem Cells in Full Thickness Cutaneous Wound Healing

2021 ◽  
Author(s):  
Jyotsana Bhatt ◽  
Amarpal . ◽  
Abas Rashid Bhat ◽  
Anuj Pratap Singh ◽  
A.M. Pawde ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Mesenchymal Stem Cells ◽  
Skin Wound ◽  
Full Thickness ◽  
Cutaneous Wound ◽  
Group Iii ◽  
Group I ◽  
Thickness Skin ◽  
Early Wound

Background: A lot of research has been done in the field of wound healing utilising stem cell therapy. This study was conducted to compare the efficacy of single and twice application of mesenchymal stem cells for cutaneous wound healing.Methods: The present study was done on 18 guinea pigs and a 2.5 X 2.5 cm2 full-thickness skin wound was created on the dorsum of each under standard anesthetic protocol. Animals were divided into 3 groups having 6 animals in each. The animals of group I were administered phosphate buffer saline (PBS) subcutaneously on day 0, group II received 1×106 mesenchymal stem cells (MSCs) in PBS subcutaneously on day 0 and group III received 1×106 MSCs in PBS subcutaneously on day 0 and again at day 3, at wound margins. Clinical, photographic, histopathological and histochemical parameters were recorded for each animal.Result: An overall gross and histopathological evaluation suggested early wound closer, better tissue granulation, early orientation of fibrocytes and collagen fibers, revealing a superior quality healing in animals of group III as compared to rest of the groups. From the above study it was concluded that twice application of MSCs leads to faster and qualitatively better healing. 

Collagen-tussah silk fibroin hybrid scaffolds loaded with bone mesenchymal stem cells promote skin wound repair in rats

2020 ◽  
Vol 109 ◽  
pp. 110611 ◽  
Author(s):  
Biling Cui ◽  
Chenchen Zhang ◽  
Bin Gan ◽  
Wenen Liu ◽  
Jiaqiang Liang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Silk Fibroin ◽  
Wound Repair ◽  
Skin Wound ◽  
Bone Mesenchymal Stem Cells ◽  
Tussah Silk Fibroin ◽  
Hybrid Scaffolds
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