scholarly journals Molecular investigation and description of Iberozospeum n. gen., including the description of one new species (Eupulmonata, Ellobioidea, Carychiidae)

Author(s):  
Jeannette Kneubühler ◽  
Adrienne Jochum ◽  
Carlos E. Prieto ◽  
Eike Neubert

AbstractThe subterranean realm of the Cantabrian-Pyrenean region of northern Spain harbours a rich diversity of Zospeum. Due to their tiny size and the difficulty of finding them alive, scarce animal material has been available for scientific investigation. Recent investigations of Zospeum shells have provided valuable, but limited insights towards our understanding of the evolutionary processes occurring within this taxon in northern Spain. In an integrative study, we investigate 57 populations of Zospeum from northern Spanish caves using two mitochondrial (COI and 16S) and two nuclear markers (H3 and 5.8 S rRNA + ITS2). Revealed is a separate radiation of the northern Spanish species for which the new genus, Iberozospeum, is proposed. The independent radiation of Dinaric Zospeum from that of northern Spain justifies the designation of Iberozospeum n. gen. Morphological evidence is provided via histological analysis of Iberozospeum vasconicum and SEM analyses of radulae of eastern Alpine, Dinaric and Iberian species. Important differences in morphological structure and character states are presented, including the first view of the sexually mature female and the presence of the giant albumen gland in an individual of the subterranean, troglobitic Carychiidae. Significant differences are revealed in superficial crystallographic structure of the columellar lamellae, the morphology of the columellar muscle and in the radula. Radular ribbon length, ribbon broadness, straightness of the ribbon base and cusp configuration are distinctive in the Iberian species. One new species is described corroborated by genetic and morphological characters.

2004 ◽  
Vol 94 (6) ◽  
pp. 525-535 ◽  
Author(s):  
G. Nicolescu ◽  
Y.-M. Linton ◽  
A. Vladimirescu ◽  
T.M. Howard ◽  
R.E. Harbach

AbstractMosquitoes of the Anopheles maculipennis group were collected in five districts of Romania (Constanta, Giurgiu, Ilfov, Mehedinti and Suceava) between March 2000 and June 2003. Two hundred and ninety-seven specimens were identified by molecular methods. Nuclear rDNA ITS2 sequences of 178 specimens were compared with GenBank sequences for nine known Palaearctic species of the group, and 119 specimens were identified using an ITS2 PCR–RFLP assay developed during the study. Five genetically distinct species of the group were identified: A. atroparvus van Thiel, A. maculipennis Meigen, A. melanoon Hackett and A. messeae Falleroni and a previously unrecognized species. The new species, herein formally described and named A. daciaesp. n., was collected in the Black Sea coastal region and plains adjacent to the Danube River in southern Romania. Anopheles daciae is most similar to and sympatric with A. messeae. It is contrasted with A. messeae and characterized on the basis of unique nuclear ITS2 and mitochondrial COI DNA sequences and morphological characters of the eggs. The larval, pupal and adults stages of the two species were also compared, but no reliable characters were found to distinguish them. It seems likely that A. daciae is more widespread in eastern Europe and the Balkan States, and could be responsible for malaria transmission in these regions that is currently attributed to A. messeae. Anopheles melanoon is reported from Romania for the first time.


PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11848
Author(s):  
Gustavo Hassemer ◽  
Elliot M. Gardner ◽  
Nina Rønsted

High-throughput sequencing, when combined with taxonomic expertise, is a powerful tool to refine and advance taxonomic classification, including at the species level. In the present work, a new species, Plantago campestris, is described out of the P. commersoniana species complex, based on phylogenomic and morphological evidence. The main morphological characters that distinguish the new species from P. commersoniana are the glabrous posterior sepals and the slightly broader leaves. The new species is known from only three localities, all in natural high-elevation grasslands in Paraná and Santa Catarina states, southern Brazil. According to the IUCN criteria new species should be assessed as Endangered (EN). We present field photographs of P. campestris and related species, and we provide an identification key to the species previously included within the circumscription of P. commersoniana.


Zootaxa ◽  
2018 ◽  
Vol 2490 (1) ◽  
pp. 55 ◽  
Author(s):  
FANG YU ◽  
YAN-ZHOU ZHANG ◽  
CHAO-DONG ZHU ◽  
LI-HONG TU

Five species of Copidosomopsis from China are reviewed, keyed and illustrated. One new species, Copidosomopsis orientalis Yu & Zhang sp. nov. is described, and C. bohemicus (Hoffer), C. meridionalis Kazmi & Hayat and C. nacoleiae (Eady) are newly recorded from China. Photomicrographs are provided to illustrate morphological characters of the species.


2018 ◽  
Vol 93 (2) ◽  
pp. 208-219
Author(s):  
J.F. Santos Neto ◽  
N.G.S. Costa ◽  
G.B. Soares ◽  
M.V. Domingues

AbstractTwo new species of Diaphorocleidus and one new species of Rhinoxenoides n. gen. are described from the gills of Acestrorhynchus falcatus (Bloch) from rivers of north-eastern Pará, Brazil. Diaphorocleidus jaymedeloyolai n. sp. is characterized by a male copulatory organ (MCO) possessing three counterclockwise coils; similar anchors with subtriangular superficial roots; a ventral bar with posteromedial projection; and hooks of pairs 1, 4 and 7 approximately three times longer than hook pair 5. Diaphorocleidus sclerocolpus n. sp. differs from its congeners by a dual-branched accessory piece articulated with the MCO and a sclerotized tubular vagina with a bottle-shaped vestibule. Rhinoxenoides n. gen. is proposed and is characterized by possessing: MCO sclerotized with clockwise coils; an accessory piece articulated to the base of MCO; a sinistroventral vaginal aperture; ventral anchor with conspicuous roots; dorsal anchor with superficial root five times longer than deep root; and absence of dorsal bar. The proposal of Rhinoxenoides n. gen. is also supported by its phylogenetic relationship with Protorhinoxenus prochilodi and species of Rhinoxenus, using 16 morphological characters, which resulted in the following hypothesis of sister-group relationships: Rhinoxenoides n. gen. [Protorhinoxenus (Rhinoxenus curimatae (R. nyttus (R. bulbovaginatus (R. guianensis, R. piranhus, R. euryxenus (R. arietinus, R. anaclaudiae)))))].


Zootaxa ◽  
2010 ◽  
Vol 2516 (1) ◽  
pp. 1 ◽  
Author(s):  
ANTOINE MANTILLERI

The genera Hoplopisthius and Carcinopisthius are reviewed. All the species are redescribed and illustrated and an updated identification key and distributional maps are provided. One new species from New Guinea is described (Hoplopisthius maximus n. sp.) and two new synonymies are proposed: Hoplopisthius celebensis Kolbe, 1892 = H. trichemerus Senna, 1892, n. syn. and Carcinopisthius lamingtoni Damoiseau, 1987 = C. forcipitiger Damoiseau, 1987, n. syn. Phylogenetic analysis using PAUP (maximum parsimony) was performed using 25 morphological characters of adults. This analysis shows the group Hoplopisthius + Carcinopisthius is monophyletic, but Carcinopisthius alone is paraphyletic. Nomenclatural changes at the generic level are made to reconcile nomenclature and phylogeny: Hoplopisthius is preserved; Carcinopisthius is downgraded to the rank of subgenus for the two oriental species H. oberthueri and H. fruhstorferi; and Pseudotaphroderes is resurrected as a third subgenus and includes all New-Guinean and Australian species.


Zootaxa ◽  
2019 ◽  
Vol 4651 (1) ◽  
pp. 85-113
Author(s):  
KOBRA HASHEMI ◽  
AKBAR KAREGAR

During a survey, 12 known and one new species of Ditylenchus Filipjev, 1936 were collected from southern provinces of Iran during 2013–2017. Ditylenchus paraparvus n. sp. is characterised by a short body length (441–543 µm), lateral field with four lines, delicate and short stylet (6–7.5 µm) with small rounded or posteriorly sloping knobs, pyriform and offset basal pharyngeal bulb, V = 67.7–76.4, short post-vulval uterine sac less than one vulval body width long, small spicules (12.5–15 µm) and almost cylindrical tail (71–90 µm) with rounded end. Morphometric data of studied species are presented and intraspecific variation of their morphometrics and morphological characters is discussed. The list of the world Ditylenchus species is updated, and a dichotomous identification key and an updated tabular compendium for 63 valid species is provided. 


1983 ◽  
Vol 14 (2) ◽  
pp. 205-233 ◽  
Author(s):  
Per Brinck

AbstractGenus-group taxon Rhombodineutus Ochs, 1926 is related to Paracyclous Ochs (Indonesia) and Callistodineutus Ochs (Melanesia), classified under Dineutus MacLeay. It occurs in New Guinea and New Britain Island, inhabiting streams and rivers primarily at low and medium altitudes where the species may occur abundantly. Most species live in the forests and have a fairly restricted range, while D. pectoralis Régimbart has passed a niche shift and adapted to exposed and exploited land and spread widely, splitting into a considerable number of races, some of which were found at an elevation of 1500-2000 m. The morphological characters are reviewed and their differentiation examined as a background for the classification of the taxon and a revision of its species which have been placed in three groups, containing 8 species and 11 subspecies. One new species and four new subspecies are described. Keys are provided to the various groups of taxa.


PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e7012 ◽  
Author(s):  
Abhijit Das ◽  
Sonali Garg ◽  
Amir Hamidy ◽  
Eric N. Smith ◽  
S. D. Biju

We describe a new species of frog in the microhylid genus Micryletta Dubois, 1987 from Northeast India based on molecular and morphological evidence. The new species, formally described as Micryletta aishani sp. nov., is phenotypically distinct from other congeners by a suite of morphological characters such as brown to reddish-brown dorsum; dorsal skin shagreened with minute spinules; snout shape nearly truncate in dorsal and ventral view; a prominent dark streak extending from tip of the snout up to the lower abdomen; ash-grey mottling along the margins of upper and lower lip extending up to the flanks, limb margins and dorsal surfaces of hand and foot; tibiotarsal articulation reaching up to the level of armpits; absence of outer metatarsal tubercles; and absence of webbing between toes. Phylogenetic relationships within the genus are inferred based on mitochondrial data and the new taxon is found to differ from all the recognised Micryletta species by 3.5–5.9% divergence in the mitochondrial 16S rRNA. The new species was found in the states of Assam, Manipur, and Tripura, from low to moderate elevation (30–800 m asl) regions lying south of River Brahmaputra and encompassing the Indo-Burma Biodiversity Hotspot. The discovery validates the presence of genus Micryletta in Northeast India based on genetic evidence, consequently confirming the extension of its geographical range, westwards from Southeast Asia up to Northeast India. Further, for nomenclatural stability of two previously known species, Microhyla inornata (= Micryletta inornata) and Microhyla steinegeri (= Micryletta steinegeri), lectotypes are designated along with detailed descriptions.


Phytotaxa ◽  
2020 ◽  
Vol 438 (4) ◽  
pp. 223-236
Author(s):  
BÁLINT DIMA ◽  
KARL SOOP

Cortinarius section Xenosmatae, originally based on solely morphological characters, was subsequently shown to contain phylogenetically distantly related species. The type species C. xenosma is a singleton, and this study aims to revise the other members of the section using combined molecular (nrDNA ITS and LSU) and morphological data. Based on phylogenetic analyses using RAxML, PhyML and Bayesian Inference and additional morphological features one new species (C. paraxenosma) and one new section (sect. Olorinati) are proposed. Furthermore sect. Carbonelli is extended and emended to include two former members of sect. Xenosmatae. A key to the species in New Zealand with xenosmatoid morphology is provided.


2009 ◽  
Vol 87 (8) ◽  
pp. 689-698 ◽  
Author(s):  
M. Okassa ◽  
M.-S. Tixier ◽  
B. Cheval ◽  
S. Kreiter

The identity of specimens belonging to the genus Euseius Wainstein, 1962 found on several trees in France is investigated. These specimens are morphologically close to Euseius stipulatus (Athias-Henriot, 1960) except for the shape of the spermatheca and the dorsal shield reticulation. The aim of the present study was to determine if these specimens belong to this latter species and if the shape of the spermatheca could be considered a reliable diagnostic character. Thirty-four characters, including idiosomal seta lengths, were measured and molecular tests (12S rDNA, COI mtDNA) were carried out. Although small, significant differences between populations of Euseius sp. were observed. The morphometric differences between E. stipulatus and Euseius sp. were very small except for the seta z2 and for the length of the ventrianal shield, as well as the length and form of the spermatheca. Molecular analyses support that Euseius sp. and E. stipulatus are two different species. Euseius sp. has been described as a new species to science in another publication under the name Euseius gallicus . Reliability of some morphological characters for species diagnosis is discussed.


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