An efficient and cost-effective method for DNA extraction from athalassohaline soil using a newly formulated cell extraction buffer

Background: Available DNA isolation methods for Plasmodium involve numerous processing steps, adding to the cost and conferring risk of contamination. Here we devise a simple and cost-effective method for direct extraction of Plasmodium DNA from dried filter paper spot (DBS), appropriate for resource-limited setups. Methods: The protocol involves simple freezing and thawing of DBS, neither involves any purification step nor any chemical reagent. The method was assessed in terms of DNA quantity, PCR detection sensitivity, time requirement, cost effectiveness, labor intensiveness and degree of shearing. The reliability of this method was confirmed by comparing it with other in use methods for Plasmodium DNA isolation. Results: Pure DNA was obtained with this method, as exemplified by the absorbance ratio (260nm /280nm) of 1.2. The protocol produced digestible, PCR-grade genomic DNA, also found to be suitable for sequencing. DNA isolated remained stable and retained its integrity after storage for one month at 4 0C. Conclusion: Our process substantiated as efficient, reproducible, simple, fast, and inexpensive. Development of this optimized freeze-thaw based DNA extraction method for malaria parasite may provide a valuable tool for molecular analysis in resource-limited setups. This is the first report of DNA extraction from DBS of Plasmodium utilizing freeze-thaw.

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An Easy, Quick and Cost Effective Method of High Quality DNA Extraction from Mungbean [Vigna radiata (L.) Wilczek] Without Liquid Nitrogen

International Journal of Current Microbiology and Applied Sciences ◽

10.20546/ijcmas.2017.609.332 ◽

2017 ◽

Vol 6 (8) ◽

pp. 2695-2703 ◽

Cited By ~ 1

Author(s):

Supriya Ambawat ◽

Ram Kumar ◽

Subaran Singh ◽

Rajesh Yadav

Keyword(s):

Liquid Nitrogen ◽

Dna Extraction ◽

Vigna Radiata ◽

Cost Effective ◽

High Quality ◽

Cost Effective Method

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On-Site DNA Extraction and Real-Time PCR for Detection of Phytophthora ramorum in the Field

Applied and Environmental Microbiology ◽

10.1128/aem.71.11.6702-6710.2005 ◽

2005 ◽

Vol 71 (11) ◽

pp. 6702-6710 ◽

Cited By ~ 88

Author(s):

J. A. Tomlinson ◽

N. Boonham ◽

K. J. D. Hughes ◽

R. L. Griffin ◽

I. Barker

Keyword(s):

Real Time ◽

Dna Extraction ◽

Real Time Pcr ◽

Cost Effective ◽

The United States ◽

Phytophthora Ramorum ◽

Taqman Assay ◽

Molecular Testing ◽

Field Independent ◽

Cost Effective Method

ABSTRACT Phytophthora ramorum is a recently described pathogen causing oak mortality (sudden oak death) in forests in coastal areas of California and southern Oregon and dieback and leaf blight in a range of tree, shrub, and herbaceous species in the United States and Europe. Due to the threat posed by this organism, stringent quarantine regulations are in place, which restrict the movement of a number of hosts. Fast and accurate diagnostic tests are required in order to characterize the distribution of P. ramorum, prevent its introduction into pathogen-free areas, and minimize its spread within affected areas. However, sending samples to a laboratory for testing can cause a substantial delay between sampling and diagnosis. A rapid and simple DNA extraction method was developed for use at the point of sampling and used to extract DNAs from symptomatic foliage and stems in the field. A sensitive and specific single-round real-time PCR (TaqMan) assay for P. ramorum was performed using a portable real-time PCR platform (Cepheid SmartCycler II), and a cost-effective method for stabilizing PCR reagents was developed to allow their storage and transportation at room temperature. To our knowledge, this is the first description of a method for DNA extraction and molecular testing for a plant pathogen carried out entirely in the field, independent of any laboratory facilities.

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A simple and cost-effective method of DNA extraction from small formalin-fixed paraffin-embedded tissue for molecular oncologic testing

BMC Clinical Pathology ◽

10.1186/1472-6890-14-30 ◽

2014 ◽

Vol 14 (1) ◽

Cited By ~ 8

Author(s):

Anthony N Snow ◽

Aaron A Stence ◽

Jonathan A Pruessner ◽

Aaron D Bossler ◽

Deqin Ma

Keyword(s):

Dna Extraction ◽

Cost Effective ◽

Formalin Fixed Paraffin ◽

Cost Effective Method ◽

Formalin Fixed Paraffin Embedded ◽

Formalin Fixed

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Using off – set handpumps — a cost-effective method to protect drinking water in rural villages

Waterlines ◽

10.3362/0262-8104.1984.019 ◽

1984 ◽

Vol 2 (4) ◽

pp. 25-26

Author(s):

Daniel Jenkins

Keyword(s):

Drinking Water ◽

Cost Effective ◽

Rural Villages ◽

Cost Effective Method

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CAD Of Anticorrosive Protection Of Steel Structures

Promyshlennoe i Grazhdanskoe Stroitel stvo ◽

10.33622/0869-7019.2019.09.18-23 ◽

2019 ◽

pp. 18-23

Keyword(s):

Protective Coating ◽

Computer Aided Design ◽

Steel Structures ◽

Cost Effective ◽

Operating Conditions ◽

Computational Tool ◽

Protection Method ◽

Cost Effective Method ◽

The Cost ◽

Aided Design

The choice of cost-effective method of anticorrosive protection of steel structures is an urgent and time consuming task, considering the significant number of protection ways, differing from each other in the complex of technological, physical, chemical and economic characteristics. To reduce the complexity of solving this problem, the author proposes a computational tool that can be considered as a subsystem of computer-aided design and used at the stage of variant and detailed design of steel structures. As a criterion of the effectiveness of the anti-corrosion protection method, the cost of the protective coating during the service life is accepted. The analysis of existing methods of steel protection against corrosion is performed, the possibility of their use for the protection of the most common steel structures is established, as well as the estimated period of effective operation of the coating. The developed computational tool makes it possible to choose the best method of protection of steel structures against corrosion, taking into account the operating conditions of the protected structure and the possibility of using a protective coating.

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