Molecular intervention of colon cancer and inflammation manifestation by tannin capped biocompatible controlled sized gold nanoparticles from Terminalia bellirica: A green strategy for pharmacological drug formulation based on nanotechnology principles

Herbal medicines are widely used worldwide and much appreciated because of their fewer side effects and the ability to fight diseases at the root cause. Active ‘phyto’ ingredients require a scientific approach and a mechanism to distribute components at the target site for better therapeutic results. Nanotechnology, on the other hand, has created new hope for cancer treatment but is still far from being proven in clinical settings. This article combines a unique approach to synthesis with the use of Pleurotus sajor-caju, followed by microwave irritation of silver and gold nanoparticles that ensures the capping of the active phyto ingredient and further enhances the effects of nanomedicine to fight colon cancer, thus opening a new era of what we call herbonanoceutics. The article also compares the characteristics and properties of silver (Au) and gold (Ag) nanoparticles synthesized by an in house developed novel microwave-assisted rapid green synthesis method. The as-prepared Ag NPs and Au NPs were compared using ultraviolet-visible spectroscopy (UV-Vis), Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), and energy dispersive spectroscopy (EDS). Our comparative study revealed that both assemblies display face-centred cubic structures (FCCs) and are nanocrystalline in nature. The advantage of the approach was that the sizes of gold and silver were identical in range with a similar distribution pattern. This has helped us to study the activity against colon cancer cell line (HCT-116) without incoherence since size plays a key role in the application. More specifically, morphological changes, cell viability, the production of reactive oxygen species (ROS) and the fragmentation of DNA have been further reported to assess better the results obtained with the two metals. Our results suggest that the newly adopted synthesis method may ensure the dual benefits from phyto ingredients which further enhances the effectiveness of advanced nanomedicine.

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Polyphenol stabilized colloidal gold nanoparticles from Abutilon indicum leaf extract induce apoptosis in HT-29 colon cancer cells

Colloids and Surfaces B Biointerfaces ◽

10.1016/j.colsurfb.2016.03.069 ◽

2016 ◽

Vol 143 ◽

pp. 499-510 ◽

Cited By ~ 59

Author(s):

Rani Mata ◽

Jayachandra Reddy Nakkala ◽

Sudha Rani Sadras

Keyword(s):

Colon Cancer ◽

Gold Nanoparticles ◽

Cancer Cells ◽

Colloidal Gold ◽

Leaf Extract ◽

Colon Cancer Cells ◽

Colloidal Gold Nanoparticles ◽

Ht 29 ◽

Abutilon Indicum

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Anticarcinogenic potential of gold nanoparticles synthesized from Trichosanthes kirilowii in colon cancer cells through the induction of apoptotic pathway

Artificial Cells Nanomedicine and Biotechnology ◽

10.1080/21691401.2019.1626412 ◽

2019 ◽

Vol 47 (1) ◽

pp. 3577-3584 ◽

Cited By ~ 4

Author(s):

Xiaodong Han ◽

Xiaojia Jiang ◽

Lanjie Guo ◽

Yongxin Wang ◽

Vishnu Priya Veeraraghavan ◽

...

Keyword(s):

Colon Cancer ◽

Gold Nanoparticles ◽

Cancer Cells ◽

Colon Cancer Cells ◽

Apoptotic Pathway ◽

Trichosanthes Kirilowii

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GREEN SYNTHESIS OF GOLD NANOPARTICLES OF ISOLATED CITRUS BIOFLAVONOID FROM ORANGE: CHARACTERIZATION AND IN VITRO CYTOTOXICITY AGAINST COLON CANCER CELL LINES COLO 320DM AND HT29

INDIAN DRUGS ◽

10.53879/id.57.08.12514 ◽

2020 ◽

Vol 57 (08) ◽

pp. 61-69

Author(s):

D. S Randive ◽

K. P. Shejawal ◽

S. D Bhinge ◽

M. A Bhutkar ◽

P. D. Patil ◽

...

Keyword(s):

Colon Cancer ◽

Gold Nanoparticles ◽

Green Synthesis ◽

Cancer Cell ◽

Orange Peel ◽

In Vitro Cytotoxicity ◽

Colon Cancer Cell ◽

Cytotoxicity Studies ◽

Ht 29

The aim of the present research was to perform green synthesis of gold nanoparticles of isolated citrus bioflavonoid from Citrus sinensis (orange) peel extract and to evaluate its anticancer potential. Methanolic extract of orange peel was obtained by Soxhlet extraction and citrus bioflavonoid was isolated by using column chromatography. Gold nanoparticles were developed by green synthesis method, wherein 1 % aqueous solution of isolated citrus bioflavonoid were mixed with 1% solution of HAuCl4 and incubated at ambient temperature for 4 to 5 hours and observed for the color change which indicated formation of nanoparticles. Obtained gold nanoparticles were evaluated by UV visible spectra, FTIR, SEM, XRD analysis and for antimicrobial potential against E coli, S.aureus and P. aeruginosa. Cytotoxicity study was carried out by using in vitro assays, namely MTT, SRB and Tryphan blue assay, against colon cancer cell line Colo 320 DM, and HT 29. results of SEM showed that nanosized particles in the range of 80-100nm were formed. Results of cytotoxicity studies revealed that CBFGNP exhibited 72.28% inhibition, against Colo320 DM whereas pure CBF showed 70.46% inhibition. Against HT 29, CBFGNP exhibited 69.79% inhibition, whereas for MTT assay and SRB assay, CBFGNP showed 80.15% and 58.29% inhibition, respectively. Moreover, CBFGNP exhibited 90.29% and 85% non viability against Colo320 DM and HT29. Based on the results, it can be concluded that gold nanoparticles of citrus bioflavonoid (CBFGNP) exhibits more cytotoxicity against Colo320 DM and HT29 as compared to pure citrus bioflavonoid when assessed by three different in vitro cytotoxicity assays.

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Efficient biogenesis of Au nanoparticles using extract of Mentha pulegium flower: Evaluation of catalytic reduction of nitroarenes and anti-human colon cancer properties in the in vitro condition

10.21203/rs.3.rs-49606/v1 ◽

2020 ◽

Author(s):

Shabnam Bovandi ◽

Maryam Shahriari ◽

Mohammad Mahdi Zangeneh

Keyword(s):

Colon Cancer ◽

Gold Nanoparticles ◽

Colorectal Adenocarcinoma ◽

Human Colon ◽

Mentha Pulegium ◽

Human Colon Cancer ◽

Au Nps ◽

X Ray ◽

Hct 116 ◽

Ht 29

Abstract Background With regards to applicative, facile, green chemical research, a bio-inspired approach is being reported for the synthesis of Au nanoparticles by using Mentha pulegium flower extract. Methods The phytochemical immobilized Au NPs were characterized by advanced physicochemical techniques like Fourier Transformed Infrared spectroscopy (FT-IR), Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), Energy Dispersive X-ray spectroscopy (EDX), and X-ray Diffraction (XRD) study. Thereafter, catalytic performance of those biomolecule functionalized Au NPs was investigated in the efficient reduction of nitroarenes over a range of substrates. To survey the anti-human colon cancer effects of gold nanoparticles, MTT assay was used on the common colon cancer cell lines i.e., colorectal adenocarcinoma (HT-29), colorectal carcinoma (HCT 116), ileocecal colorectal adenocarcinoma (HCT-8 [HRT-18]), and Burkitt's lymphoma (Ramos.2G6.4C10). Results The conversion was achieved in short reaction time with good to excellent yields in association with outstanding turnover frequency (TOF). In addition, the nanocomposite catalyst was easily recovered and recycled for 12 successive times without noticeable decrease in catalytic activity. Gold nanoparticles had high anti-colon cancer activities dose-dependently against HT-29, HCT 116, HCT-8 [HRT-18], and Ramos.2G6.4C10 cell lines. The best result of anti-colon cancer effects was seen in the case of the HCT 116 cell line. Conclusions It looks gold nanoparticles can be used for the treatment of several types of colon cancers in human.

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