scholarly journals Correction to: Microbial spectrum, antibiotic susceptibility profile, and biofilm formation of diabetic foot infections (2014–18): a retrospective multicenter analysis

3 Biotech ◽  
2021 ◽  
Vol 11 (9) ◽  
Author(s):  
Fakhria A. Al-Joufi ◽  
Khalid M. Aljarallah ◽  
Soheir A. Hagras ◽  
Ibrahim M. Al Hosiny ◽  
Mounir M. Salem‑Bekhit ◽  
...  
Keyword(s):  
Diabetic Foot ◽  
Antibiotic Susceptibility ◽  
Biofilm Formation ◽  
Diabetic Foot Infections ◽  
Multicenter Analysis ◽  
Microbial Spectrum

scholarly journals Microbial spectrum, antibiotic susceptibility profile, and biofilm formation of diabetic foot infections (2014–18): a retrospective multicenter analysis

3 Biotech ◽  
2020 ◽  
Vol 10 (7) ◽  
Author(s):  
Fakhria A. Al-Joufi ◽  
Khalid M. Aljarallah ◽  
Soheir A. Hagras ◽  
Ibrahim M. Al Hosiny ◽  
Mounir M. Salem-Bekhit ◽  
...  
Keyword(s):  
Diabetic Foot ◽  
Antibiotic Susceptibility ◽  
Biofilm Formation ◽  
Diabetic Foot Infections ◽  
Multicenter Analysis ◽  
Microbial Spectrum

scholarly journals Spectral and Antibiotic Susceptibility of Pathogens Isolated from Saudi Patients with Diabetic Foot Infections

2021 ◽  
Vol 12 (1) ◽  
pp. 16-20
Author(s):  
Samiah Hamad S Al-Mijalli
Keyword(s):  
Staphylococcus Aureus ◽  
Diabetic Foot ◽  
Antibiotic Susceptibility ◽  
Empirical Therapy ◽  
Multidrug Resistant ◽  
Foot Ulcers ◽  
Sensitivity Testing ◽  
Cross Sectional ◽  
Diabetic Foot Infections ◽  
Microbiological Methods

Diabetic foot infections (DFIs) are a significant health issue and a common complication among patients with diabetes. To develop antibiotic therapy for these high-risk patients, the current study evaluates the scope of DFIs and identifies the causing microbes. It also measures spectrum and antibiotic susceptibility of the pathogens isolated from adults with DFIs in Saudi Arabia. To achieve the study objectives, a cross-sectional study was implemented and the baseline characteristics for 44 patients with DFIs were defined. Optimal aerobic and anaerobic microbiological techniques were utilized to culture specimens isolated from infected foot ulcers. The standard microbiological methods were employed to identify the bacterial isolates and antibiotic susceptibility testing was conducted following the procedures of the Clinical and Laboratory Standards Institute (CLSI). Results showed that 12 microorganisms were isolated from the participants’ diabetic foot ulcers. Staphylococcus Aureus was ranked first because it appeared in 29 (65.9%) cases. Streptococcus Agalactiae was ranked second and multi-microbial infections were also found. Most of the organisms were susceptible to Vancomycin, Ciprofloxacin, and Cefalexin, but they were resistant to Methicillin, Gentamicin, and Ampicillin antibiotics. Staphylococcus Aureus was most sensitive to Ciprofloxacin, while it was resistant to Methicillin. About 10% of the isolates were multidrug-resistant. The study concludes that while Vancomycin should be used empirically for Gram-positive isolates, Ciprofloxacin can be taken into consideration for most of the Gram-negatives aerobes. Based on including various microorganisms and the advent of multidrug-resistant strains, proper culture and sensitivity testing are necessary prior to the empirical therapy.

scholarly journals Hypertonic glucose inhibits growth and attenuates virulence factors of multidrug-resistant Pseudomonas aeruginosa

2020 ◽  
Author(s):  
Tao Chen ◽  
Ye Xu ◽  
Wenya Xu ◽  
Wenli Liao ◽  
Chunquan Xu ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Virulence Factors ◽  
Diabetic Foot ◽  
Biofilm Formation ◽  
Chronic Wound ◽  
Multidrug Resistant ◽  
Wound Infections ◽  
Hypertonic Glucose ◽  
Diabetic Foot Infections ◽  
Chronic Wound Infections

Abstract Background: Pseudomonas aeruginosa is the most common Gram-negative pathogen responsible for chronic wound infections, such as diabetic foot infections, and further exacerbates the treatment options and cost of such conditions. Hypertonic glucose, a commonly used prolotherapy solution, can accelerate the proliferation of granulation tissue and improve microcirculation in wounds. However, the action of hypertonic glucose on bacterial pathogens that infect wounds is unclear. In this study, we investigated the inhibitory effects of hypertonic glucose on multidrug-resistant P. aeruginosa strains isolated from diabetic foot infections. Hypertonic glucose represents a novel approach to control chronic wound infections caused by P. aeruginosa. Results: Four multidrug-resistant P. aeruginosa clinical strains isolated from diabetic foot ulcers from a tertiary hospital in China and the reference P. aeruginosa PAO1 strain were studied. Hypertonic glucose significantly inhibited the growth, biofilm formation, and swimming motility of P. aeruginosa clinical strains and PAO1. Furthermore, hypertonic glucose significantly reduced the production of pyocyanin and elastase virulence factors in P. aeruginosa. The expression of major quorum sensing genes (lasI, lasR, rhlI, and rhlR) in P. aeruginosa were all downregulated in response to hypertonic glucose treatment. In a Galleria mellonella larvae infection model, the administration of hypertonic glucose was shown to increase the survival rates of larvae infected by P. aeruginosa strains (3/5).Conclusions: Hypertonic glucose inhibited the growth, biofilm formation, and swimming motility of P. aeruginosa, as well as reduced the production of virulence factors and quorum sensing gene expression. Further studies that investigate hypertonic glucose therapy should be considered in treating chronic wound infections.

scholarly journals Antibiotic Susceptibility Profiles of Bacteria from Diabetic Foot Infections in Selected Teaching Hospitals in Southwestern Nigeria

2018 ◽  
Vol 4 (1) ◽  
pp. 1-13 ◽  
Author(s):  
Olorunjuwon O Bello ◽  
Edward O Oyekanmi ◽  
Babatunde A Kelly ◽  
Olakunle O Mebude ◽  
Temitope K Bello
Keyword(s):  
Diabetic Foot ◽  
Antibiotic Susceptibility ◽  
Bacterial Species ◽  
Foot Ulcer ◽  
Bacterial Isolates ◽  
Southwestern Nigeria ◽  
Mannitol Salt Agar ◽  
Gram Negative ◽  
Diabetic Foot Infections ◽  
Susceptibility Profiles

One of the serious complications associated with diabetes is foot ulcer and this condition affects the quality of life in patients in all classes, races and ages. Chronic wounds are prone to colonization by wide array of microorganisms which could be extremely hazardous to patients if effective and timely therapeutic intervention is not made. This study was conducted to determine the antibiotic susceptibility profiles of bacteria from diabetic foot infections in southwestern Nigeria. Samples were collected from a total of 142 diabetic foot ulcer patients with moistened sterile cotton swabs. Nutrient agar, Mac-Conkey agar, blood agar and mannitol salt agar media were used for the isolation of total viable bacteria, Gram-negative non-spore forming lactose fermenters, fastidious bacteria and staphylococci, respectively. Morphological and biochemical characteristics of pure cultures were determined in accordance with standard laboratory criteria. API 20 E and API 20 NE were used for the confirmation of identity of the bacterial isolates. The disc diffusion technique was employed for the determination of antibiotic susceptibility of bacterial isolates in accordance with standard procedures. The antibiotics investigated included amikacin, amoxicillin, ampicillin, ceftazidime, cefazolin, ceftriaxone, chloramphenicol, ciprofloxacin, clindamycin, gentamicin, imipenem, linezolid, methicillin, netilmicin, ofloxacin, oxacillin, penicillin, piperacilin, sulfamethoxazole, trimethoprim and vancomycin. One hundred and seventy-seven isolates were encountered and these were characterized into eleven bacterial species. These included Staphylococcus aureus (22.03%), Pseudomonas aeruginosa (16.95%), Staphylococcus epidermidis (12.43%), Proteus mirabilis (8.48%), Klebsiella pneumoniae (7.91%), E. coli (7.35%), Staphylococcus saprophyticus (6.78%), Streptococcus pyogenes (5.65%), Morganella morganii (5.09%), Citrobacter freundii (4.52%) and Acinetobacter baumannii (2.83%). Gram-negative bacteria showed 76.99% susceptibility to the antibiotics while 22.85% was resistant. Gram-positive bacteria showed 93.75% susceptibility and 5.01% resistance to the antibiotics. This study revealed that there is no definite aetiologic bacterial agent for diabetic foot infections and many of the associated bacteria are sensitive to certain antibiotics.

scholarly journals The effect of biofilm formation on the outcome therapy of diabetic foot infections (DFIs) patients in the outpatient clinic and inpatient ward of Dr Sardjito General Hospital Yogyakarta

2021 ◽  
pp. 172-177
Author(s):  
Ika Puspitasari ◽  
Titik Nuryastuti ◽  
Rizka Humardewayanti Asdie ◽  
Hemi Sinorita ◽  
Nusaibah Umaroh ◽  
...  
Keyword(s):  
General Hospital ◽  
Diabetic Foot ◽  
Biofilm Formation ◽  
Communicable Disease ◽  
Therapy Outcome ◽  
Exclusion Criteria ◽  
Inpatient Ward ◽  
Diabetic Foot Infections ◽  
Culture And Sensitivity ◽  
Non Communicable Disease

Introduction: Diabetes is a non-communicable disease with incidence rate of about 1.5 – 2.3% per annum with the most complication is Diabetic Foot Infections (DFIs). Objective: This research was conducted to describe the bacteria responsible for biofilm formation and its ability to cause DFIs in biofilm formation at Dr. Sardjito General Hospital as well as the therapy outcome. Methods: This research was conducted from September to November 2017. Specimens of samples were obtained from wound swabs of DFIs patients who met the inclusion and exclusion criteria (31 outpatients and 15 inpatients), and were then tested for culture and sensitivity and their ability to form biofilms. Results: The DFIs with the biofilm-producing bacteria (weak to moderate) have a different outcome compared to DFIs patients without biofilms.

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