Growth promotion of Vigna mungo (L.) by Pseudomonas spp. exhibiting auxin production and ACC-deaminase activity

2011 ◽  
Vol 62 (1) ◽  
pp. 411-417 ◽  
Author(s):  
Shahzadi Noreen ◽  
Basharat Ali ◽  
Shahida Hasnain
Keyword(s):  
Growth Promotion ◽  
Acc Deaminase ◽  
Vigna Mungo ◽  
Pseudomonas Spp ◽  
Auxin Production

scholarly journals Genomic Diversity of Two Hydrocarbon-Degrading and Plant Growth-Promoting Pseudomonas Species Isolated from the Oil Field of Bóbrka (Poland)

Genes ◽  
2019 ◽  
Vol 10 (6) ◽  
pp. 443 ◽  
Author(s):  
Valeria Imperato ◽  
Miguel Portillo-Estrada ◽  
Breanne M. McAmmond ◽  
Yorben Douwen ◽  
Jonathan D. Van Hamme ◽  
...  
Keyword(s):  
Plant Growth ◽  
Growth Promotion ◽  
Acc Deaminase ◽  
Proton Transfer Reaction ◽  
Degradation Pathway ◽  
Oil Field ◽  
Genomic Diversity ◽  
Pseudomonas Spp ◽  
Degrading Bacteria

Hydrocarbon-degrading bacteria are important resources for use in phytoremediation applications. Yet, for many hydrocarbonoclastic strains the genetic information regarding pollutant degradation and detoxification has not been thoroughly revealed. In this study, hydrocarbon-degrading bacteria were isolated from a long-term oil-polluted soil in Bóbrka, Poland. Pseudomonas spp. was the most dominant species. Of all 69 isolated strains tested in the laboratory using qualitative biochemical assays, 61% showed the capability to use diesel as sole carbon source, 33% could produce indole, 19% produced siderophores, 36% produced organic acids, and 54% were capable of producing 1-aminocyclopropane-1-carboxylate (ACC)-deaminase. From all morphologically and genetically different strains, two representative Pseudomonas spp., strain VI4.1 and VI4T1, were selected for genome sequencing. Genomic analyses indicated the presence of the full naphthalene dioxygenase operon (plasmid and chromosomal), of genes involved in the degradation of BTEX compounds (Benzene, Toluene, Ethylbenzene, Xylene) and alkanes (alkB gene) as well as the anthranilate degradation pathway (strain VI4T1) and terephthalate dioxygenase protein (strain VI4.1). Proton transfer reaction time-of-flight mass spectrometry (PTR-TOF-MS) analyses confirmed naphthalene and BTEX degradation within seven days. Motility, resistance to abiotic stresses, high and low temperatures, low pH, and salinity were confirmed at the genetic level and experimentally verified. The presence of multiple degradative and plant growth promotion genes, together with the in vitro experimental evidence, indicates the high value of these two strains and their potential use for sustainable site clean-up.

scholarly journals Screening, Identification and Growth-Promotion Products of Multifunctional Bacteria in a Chinese Fir Plantation

Forests ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 120
Author(s):  
Guangyu Zhao ◽  
Yihui Wei ◽  
Jiaqi Chen ◽  
Yuhong Dong ◽  
Lingyu Hou ◽  
...  
Keyword(s):  
High Efficiency ◽  
Growth Promotion ◽  
Nitrogenase Activity ◽  
Acc Deaminase ◽  
Inorganic Phosphorus ◽  
Chinese Fir ◽  
Biochemical Tests ◽  
Growth Promoting ◽  
Chinese Fir Plantation ◽  
Physiological And Biochemical

Purpose: This research was aimed to screen and identify multifunctional phosphorus-dissolving bacteria of a Chinese fir (Cunninghamia lanceolata) plantation and study its phosphorus-dissolving characteristics in order to provide strain resources and a theoretical basis for developing the appropriate bacterial fertilizer of a Chinese fir plantation. Methods: First, phosphorus-dissolving bacteria were isolated from the woodland soil of a Chinese fir plantation by Pikovskava inorganic phosphorus medium (PVK). Then, some growth-promoting indicators of primary screening strains were determined, including the capacity of phosphorus-solubilized, nitrogenase activity, 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity, production of indole-3-acetic acid (IAA), secretion of iron carrier and so on. Finally, the screening multifunctional phosphorus-dissolving bacteria were identified, which were combined with colony characteristics, physiological and biochemical tests and molecular biotechnology. Results: (1) Thirteen phosphorus-dissolving bacteria were isolated and screened in total, and P5 (195.61 mg·L−1) had the strongest capacity of phosphorus-solubilized. Five phosphorus-dissolving bacteria were provided with nitrogenase activity, and the highest activity of nitrogenase was P10 and P5 (71.90 C2H4 nmol·mL−1·h−1 and 71.00 C2H4 nmol·mL−1·h−1, respectively). Four strains were provided with ACC deaminase activity, and the highest activity of ACC deaminase was P5 and P9, (0.74 μmol·mg−1·h−1 and 0.54 μmol·mg−1·h−1, respectively). Most strains could secrete IAA, and three strains of bacteria had a strong secretory ability, which could secrete IAA with a concentration greater than 15 mg·mL−1, and P5 was 18.00, P2 was 17.30, P6 was 15.59 (mg·mL−1). P5 produced carriers of iron better than others, and the ratio of the diameter of the iron production carrier ring to the diameter of the colony was 1.80, respectively, which was significantly higher than other strains. Combining all kinds of factors, P5 multifunctional phosphorus-dissolving bacteria were screened for eventual further study. (2) Strain P5 was identified as Burkholderia ubonensis, based on the colony characteristics, physiological and biochemical tests, 16SrDNA sequence analysis and phylogenetic tree construction. Conclusion: P5 has a variety of high-efficiency growth-promoting capabilities, and the ability to produce IAA, ACC deaminase activity and siderophore performance are significantly higher than other strains, which had great potential in the development of microbial fertilizer.

scholarly journals Quorum Sensing System Affects the Plant Growth Promotion Traits of Serratia fonticola GS2

2020 ◽  
Vol 11 ◽  
Author(s):  
Byung Kwon Jung ◽  
Jerald Conrad Ibal ◽  
Huy Quang Pham ◽  
Min-Chul Kim ◽  
Gun-Seok Park ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Plant Growth ◽  
Plant Growth Promotion ◽  
Biofilm Formation ◽  
Growth Promotion ◽  
Acc Deaminase ◽  
Deletion Mutants ◽  
Phenotypic Data ◽  
Collective Behaviors ◽  
Pgp Activities

Quorum sensing (QS) enables bacteria to organize gene expression programs, thereby coordinating collective behaviors. It involves the production, release, and population-wide detection of extracellular signaling molecules. The cellular processes regulated by QS in bacteria are diverse and may be used in mutualistic coordination or in response to changing environmental conditions. Here, we focused on the influence of the QS-dependent genes of our model bacterial strain Serratia fonticola GS2 on potential plant growth promoting (PGP) activities including indole-3-acetic acid (IAA) production, 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity, and biofilm formation. Based on genomic and phenotypic experimental data we identified and investigated the function of QS genes in the genome of the model strain. Our gene deletion study confirmed the biological functionality of the QS auto-inducer (gloI) and receptor (gloR) on potential PGP activities of GS2. A transcriptomic approach was also undertaken to understand the role of QS genes in regulation of genes primarily involved in PGP activities (IAA, ACC deaminase activity, and biofilm formation). Both transcriptomic and phenotypic data revealed that the QS-deletion mutants had considerably less PGP activities, as compared to the wild type. In addition, in vivo plant experiments showed that plants treated with GS2 had significantly higher growth rates than plants treated with the QS-deletion mutants. Overall, our results showed how QS-dependent genes regulate the potential PGP activities of GS2. This information may be helpful in understanding the relationship between QS-dependent genes and the PGP activity of bacteria, which aid in the production of practical bio-fertilizers for plant growth promotion.

scholarly journals Exploitation of endophytic Pseudomonas sp. for plant growth promotion and colonization in rice

2017 ◽  
Vol 9 (3) ◽  
pp. 1310-1316
Author(s):  
Gurjot Kaur ◽  
Poonam Sharma ◽  
Deepika Chhabra ◽  
Kailash Chand ◽  
Gurjit Singh Mangat
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Growth Promotion ◽  
Acc Deaminase ◽  
Pseudomonas Sp ◽  
Bacterial Isolates ◽  
Pgp Traits ◽  
P Solubilization ◽  
Fluorescent Pigment

The present investigation was carried out to exploit bacterial endophytes associated with root and leaf tissue of rice plant for plant growth promotion (PGP) and colonization study in vitro. Total 10 endophytic bacterial isolates (Pseudomonas sp.) were evaluate for PGP traits like P solubilization, production of Indole acetic acid (IAA), siderophore, ACC deaminase, protease, cellulase, fluorescent pigment, urease and denitrification activity. Out of 10 endophytic bacteria 30 %, 60 %, 20 %, 70 %, 10 % and 10 % were positive for siderophore, protease, cellulase, fluorescent pigment, urease and denitrification respectively. Maximum IAA production was recorded with isolate LRBLE7 (18.8 μgml-1) followed by LRBRE4 (16.0 μgml-1) and maximum P-solubilization was recorded with isolate LRBRE4 (5.8 mg 100 ml-1) followed by LRBLE7 (4.4 mg 100 ml-1). ACC deaminase production was recorded with isolate LRBLE6 (O.D=0.352 nm) followed by LRBRE5 (O.D=0.324nm). Three potential isolates (LRBRE4, LRBRE6 and LRBLE7) were selected on the basis of multiple PGP traits and were subjected to colonization study of rice seedling in vitro. Potential bacterial isolates can be exploited for improving growth and productivity in rice under sustainable management system.

Transcriptional regulation of the Enterobacter cloacae UW4 1-aminocyclopropane-1-carboxylate (ACC) deaminase gene (acdS)

2001 ◽  
Vol 47 (4) ◽  
pp. 359-367 ◽  
Author(s):  
Jiping Li ◽  
Bernard R Glick
Keyword(s):  
Transcriptional Regulation ◽  
Luciferase Activity ◽  
Growth Promotion ◽  
Regulatory Protein ◽  
Acc Deaminase ◽  
Enterobacter Cloacae ◽  
Upstream Region ◽  
Luciferase Expression ◽  
Luciferase Gene ◽  
The One

Based on DNA sequence analysis and 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity, the region of DNA immediately upstream of the Enterobacter cloacae UW4 ACC deaminase gene (acdS) contains several features that appear to be involved in its transcriptional regulation. In the present study, the 5' upstream region of acdS was cloned into the promoter-probe vector, pQF70, which carries the promoterless luciferase gene (luxAB), and luciferase expression was monitored. The data obtained from studying the expression of the luciferase gene showed that (i) a leucine responsive regulatory protein (LRP)-like protein encoded within the upstream region is located on the opposite strand from acdS under the control of a promoter stronger than the one responsible for acdS transcription, (ii) luciferase gene expression required both ACC and the LRP-like protein, (iii) luciferase expression was increased three-fold under anaerobic conditions, consistent with the involvement of a fumarate-nitrate reduction (FNR)-like regulatory protein box within the upstream region, and (iv) the addition of leucine to the growth medium decreased luciferase activity in the presence of ACC and increased luciferase activity in the absence of ACC, consistent with leucine acting as a regulator of the expression of the LRP-like protein.Key words: plant growth promotion, ethylene, ACC deaminase, regulation, Enterobacter cloacae.

Pseudomonads contribute to regulation ofPratylenchus penetrans(Nematoda) populations on apple

2018 ◽  
Vol 64 (11) ◽  
pp. 775-785 ◽  
Author(s):  
Tristan T. Watson ◽  
Tom A. Forge ◽  
Louise M. Nelson
Keyword(s):  
Pseudomonas Fluorescens ◽  
Protease Activity ◽  
Fungal Pathogens ◽  
Plant Biomass ◽  
Growth Promotion ◽  
In Vitro Growth ◽  
Pratylenchus Penetrans ◽  
Pseudomonas Spp ◽  
Orchard Soil

Inoculation with antagonistic soil microorganisms has shown potential to suppress replant disease of apple in orchard soils. Pseudomonas spp. may have the potential to reduce Pratylenchus penetrans populations on apple. Pseudomonas spp. were isolated from the rhizosphere of sweet cherry and screened for antagonistic characteristics. Two highly antagonistic Pseudomonas isolates, P10-32 and P10-42, were evaluated for growth promotion of apple seedlings, suppression of P. penetrans populations, and root colonization in soil from three orchards. During the isolate screening, Pseudomonas fluorescens P10-32 reduced in vitro growth of fungal pathogens, had protease activity, had capacity to produce pyrrolnitrin, suppressed P. penetrans populations, and increased plant biomass. Pseudomonas fluorescens P10-42 reduced in vitro growth of fungal pathogens, had protease activity, suppressed P. penetrans populations, and increased plant biomass. In potted orchard soil, inoculating apple with P. fluorescens P10-32 suppressed P. penetrans populations in one of the three soils examined. Inoculation with P. fluorescens P10-42 improved plant growth in two of the soils and suppressed P. penetrans abundance in one soil. In one of the soils, P. fluorescens P10-42 was detected on the roots 56 days postinoculation. Overall, we conclude that Pseudomonas spp. play a role in suppressing P. penetrans on apple in orchard soil.

scholarly journals Enhancing the 1-Aminocyclopropane-1-Carboxylate Metabolic Rate of Pseudomonas sp. UW4 Intensifies Chemotactic Rhizocompetence

2020 ◽  
Vol 8 (1) ◽  
pp. 71
Author(s):  
Xiyang Gao ◽  
Tao Li ◽  
Wenliang Liu ◽  
Yan Zhang ◽  
Di Shang ◽  
...  
Keyword(s):  
Metabolic Rate ◽  
Growth Promotion ◽  
Gene Promoter ◽  
Acc Deaminase ◽  
Dry Weight ◽  
Chemotactic Activity ◽  
Pseudomonas Sp ◽  
Rhizosphere Colonization ◽  
Synthetic Promoters ◽  
Acds Gene

1-aminocyclopropane-1-carboxylic acid (ACC) is a strong metabolism-dependent chemoattractant for the plant beneficial rhizobacterium Pseudomonas sp. UW4. It is unknown whether enhancing the metabolic rate of ACC can intensify the chemotaxis activity towards ACC and rhizocompetence. In this study, we selected four promoters to transcribe the UW4 ACC deaminase (AcdS) gene in the UW4 ΔAcdS mutant. PA is the UW4 AcdS gene promoter, PB20, PB10 and PB1 are synthetic promoters. The order of the AcdS gene expression level and AcdS activity of the four strains harboring the promoters were PB20 > PA > PB10 > PB1. Interestingly, the AcdS activity of the four strains and their parent strain UW4 was significantly positively correlated with their chemotactic activity towards ACC, rhizosphere colonization, roots elongation and dry weight promotion. The results released that enhancing the AcdS activity of PGPRenable them to achieve strong chemotactic responses to ACC, rhizocompetence and plant growth promotion.

scholarly journals Exploring the Multi-trait Plant Growth Promotion Capability of Commercial Liquid Biofertilizers Isolates

2015 ◽  
Vol 9 (3) ◽  
pp. 24-37 ◽  
Author(s):  
Mohammed Faisal Ansari ◽  
Devayani R. Tipre ◽  
Shailesh R. Dave
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Phosphate Solubilization ◽  
Growth Promotion ◽  
Acc Deaminase ◽  
Multiple Traits ◽  
The Past ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Field Efficiency

Organic farming is gaining popularity where bio-inoculants could play a key role in promoting the growth of plants. The liquid biofertilizers concept is new to farmers and developed recently. Lots of liquid biofertilizers formulations and field efficiency were shown in the past by various researchers, but the plant growth promoting (PGP) efficiency of the liquid biofertilizers isolates were not reported till date. In the present work 6 different commercially available liquid biofertilizers were used to isolate the organism. These isolated cultures were used to study their PGP efficiency with respect to phosphate solubilization and production of EPS, IAA, siderophore, ammonia, chitinase, ACC-deaminase and HCN. The phosphate solubilization was shown up to 303 g/ml by APS isolate. EPS production was shown by using different C sources and production up to 24 g/l was shown by studied isolated. Most of the organisms studied were able to produce IAA and highest production was shown up to 20 g/ml. More than 65% studied isolates showed siderophore and ACC-deaminase production. The present study shows that the commercial liquid biofertilizer isolates possess multiple traits of plant growth promotion. DOI: http://dx.doi.org/10.3126/ijls.v9i3.12463   International Journal of Life Sciences 9 (3): 2015; 24-37

Sign in / Sign up

Export Citation Format

Share Document