Monitoring of HTLV-1-associated diseases by proviral load quantification using multiplex real-time PCR

2022 ◽  
Author(s):  
Evandra Strazza Rodrigues ◽  
Suellen Salustiano ◽  
Elaine Vieira Santos ◽  
Svetoslav Nanev Slavov ◽  
Virgínia Picanço-Castro ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Proviral Load ◽  
Associated Diseases

Significance of HTLV-1 proviral load quantification by real-time PCR as a surrogate marker for HTLV-1-infected cell count

2003 ◽  
Vol 25 (2) ◽  
pp. 111-117 ◽  
Author(s):  
S. Kamihira ◽  
N. Dateki ◽  
K. Sugahara ◽  
T. Hayashi ◽  
H. Harasawa ◽  
...  
Keyword(s):  
Infected Cell ◽  
Cell Count ◽  
Real Time ◽  
Real Time Pcr ◽  
Proviral Load ◽  
Surrogate Marker

Use of synthetic oligonucleotides for determination of HTLV‐1 proviral load by real‐time PCR: a helpful alternative approach in the clinical management

2020 ◽  
Vol 129 (3) ◽  
pp. 768-774
Author(s):  
L.M. Bandeira ◽  
M.A.M. Puga ◽  
V.S. Paula ◽  
L.H.F. Demarchi ◽  
G.G.C. Lichs ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Clinical Management ◽  
Proviral Load ◽  
Alternative Approach ◽  
Synthetic Oligonucleotides

scholarly journals Quantification of Proviral Load of Human Immunodeficiency Virus Type 2 Subtypes A and B Using Real-Time PCR

2001 ◽  
Vol 39 (12) ◽  
pp. 4264-4268 ◽  
Author(s):  
F. Damond ◽  
D. Descamps ◽  
I. Farfara ◽  
J. N. Telles ◽  
S. Puyeo ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Real Time ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Real Time Pcr ◽  
Proviral Load ◽  
Immunodeficiency Virus

Quantitation of HTLV-I proviral load by a real-time PCR assay using SYBR Green: Comparison of two methods for DNA isolation

2010 ◽  
Vol 170 (1-2) ◽  
pp. 160-164 ◽  
Author(s):  
Natalia Andrea Altamirano ◽  
Carlos Rocco ◽  
Paula Aulicino ◽  
Luisa Sen ◽  
Andrea Mangano
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Proviral Load ◽  
Dna Isolation ◽  
Sybr Green ◽  
Pcr Assay

scholarly journals Histological Lesions and Replication Sites of PCV3 in Naturally Infected Pigs

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1520
Author(s):  
Elisa Rigo De Conti ◽  
Talita Pilar Resende ◽  
Lacey Marshall-Lund ◽  
Albert Rovira ◽  
Fabio Augusto Vannucci
Keyword(s):  
In Situ Hybridization ◽  
Lymph Nodes ◽  
Real Time ◽  
Real Time Pcr ◽  
Systemic Vasculitis ◽  
Tissue Type ◽  
Lymphoid Tissues ◽  
Associated Diseases ◽  
Spleen And Lymph Nodes

Porcine circovirus type 3 (PCV3) has been recently described as a potential cause of abortions and systemic vasculitis in pigs. Although the virus has been detected by real-time PCR in several porcine tissues from countries worldwide, PCV3-associated diseases have not been satisfactorily clarified. The objective of this study was to investigate the association between the presence of PCV3 mRNA detected by in situ hybridization (ISH) within histological lesions and PCV3 DNA detected by real-time PCR in naturally infected pigs. A total of 25 PCV3 PCR-positive cases were analyzed. Formalin-fixed tissues from these cases were evaluated for histologic lesions and for ISH-RNA positive signals for PCV3. The most frequent tissue type with histopathologic lesions was heart, 76.2%, with lymphoplasmacytic myocarditis and epicarditis as the most frequent lesions observed. Lymphoplasmacytic interstitial pneumonia was also a frequent finding, 47.6%. There were also lesions in kidney, liver, spleen and lymph nodes. PCV3-ISH-RNA positive signals were mostly observed in association with lymphoplasmacytic inflammatory infiltrate in various tissues, including arteries. Based on our results, the minimum set of specimens to be submitted for histopathology and mRNA in situ hybridization to confirm or exclude a diagnosis of PCV3 are heart, lung and lymphoid tissues (i.e., spleen and lymph nodes), especially for differential diagnosis related with PCV2-associated diseases.

scholarly journals HTLV-1-Associated Myelopathy/Tropical Spastic Paraparesis Is Not Associated with SNP rs12979860 of the IL-28B Gene

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Antonio C. R. Vallinoto ◽  
Bárbara Brasil Santana ◽  
Keyla S. G. Sá ◽  
Tuane C. S. Ferreira ◽  
Rita Catarina M. Sousa ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Proviral Load ◽  
Spastic Paraparesis ◽  
Cell Subset ◽  
Tropical Spastic Paraparesis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Asymptomatic Group ◽  
Positive Correlation ◽  
Asymptomatic Subjects

The present study investigated the association between the rs12979860 polymorphism in the IL-28B gene and HTLV-1 infection as well as the development of HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). HTLV-1-infected patients (26 HAM/TSP symptomatic and 53 asymptomatic) and 300 seronegative healthy controls were investigated. Plasma levels of the cytokines TNF-α, TNF-β, IL-8, IL-10, IL-6, and IFN-γfrom infected patients were measured using an indirect enzyme-linked immunosorbent assay. The HTLV proviral load was measured using a real-time PCR assay, and T-cell subset counts were determined by flow cytometry. Real-time PCR was used to genotype the rs12979860 SNP. The allelic and genotypic distributions displayed no significant differences among the investigated groups. No significant association between the serum cytokine levels and the presence of the rs12979860 SNP in symptomatic and asymptomatic subjects was observed. A positive correlation (p=0.0015) between TNF-βand IFN-γwas observed in the asymptomatic group, but a positive correlation was only observed (p=0.0180) between TNF-αand IL-6 in the HAM/TSP group. The proviral load was significantly higher in HAM/TSP patients than in asymptomatic subjects. The present results do not support a previous report indicating an association between the SNP rs12979860 and HAM/TSP outcome.

Multiplex real-time PCR for the detection and quantitation of HTLV-1 and HTLV-2 proviral load: Addressing the issue of indeterminate HTLV results

2011 ◽  
Vol 52 (1) ◽  
pp. 38-44 ◽  
Author(s):  
Allison Waters ◽  
André L.A. Oliveira ◽  
Suzie Coughlan ◽  
Carla de Venecia ◽  
Doris Schor ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Proviral Load
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