scholarly journals HTLV-1-Associated Myelopathy/Tropical Spastic Paraparesis Is Not Associated with SNP rs12979860 of the IL-28B Gene

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Antonio C. R. Vallinoto ◽  
Bárbara Brasil Santana ◽  
Keyla S. G. Sá ◽  
Tuane C. S. Ferreira ◽  
Rita Catarina M. Sousa ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Proviral Load ◽  
Spastic Paraparesis ◽  
Cell Subset ◽  
Tropical Spastic Paraparesis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Asymptomatic Group ◽  
Positive Correlation ◽  
Asymptomatic Subjects

The present study investigated the association between the rs12979860 polymorphism in the IL-28B gene and HTLV-1 infection as well as the development of HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). HTLV-1-infected patients (26 HAM/TSP symptomatic and 53 asymptomatic) and 300 seronegative healthy controls were investigated. Plasma levels of the cytokines TNF-α, TNF-β, IL-8, IL-10, IL-6, and IFN-γfrom infected patients were measured using an indirect enzyme-linked immunosorbent assay. The HTLV proviral load was measured using a real-time PCR assay, and T-cell subset counts were determined by flow cytometry. Real-time PCR was used to genotype the rs12979860 SNP. The allelic and genotypic distributions displayed no significant differences among the investigated groups. No significant association between the serum cytokine levels and the presence of the rs12979860 SNP in symptomatic and asymptomatic subjects was observed. A positive correlation (p=0.0015) between TNF-βand IFN-γwas observed in the asymptomatic group, but a positive correlation was only observed (p=0.0180) between TNF-αand IL-6 in the HAM/TSP group. The proviral load was significantly higher in HAM/TSP patients than in asymptomatic subjects. The present results do not support a previous report indicating an association between the SNP rs12979860 and HAM/TSP outcome.

Usefulness of Proviral Load Measurement for Monitoring of Disease Activity in Individual Patients with Human T-Lymphotropic Virus Type I-Associated Myelopathy/Tropical Spastic Paraparesis

2003 ◽  
Vol 9 (1) ◽  
pp. 29-35 ◽  
Author(s):  
Norihiro Takenouchi ◽  
Yoshihisa Yamano ◽  
Koichiro Usuku ◽  
Mitsuhiro Osame ◽  
Shuji Izumo
Keyword(s):  
Disease Activity ◽  
Virus Type ◽  
Proviral Load ◽  
Spastic Paraparesis ◽  
Tropical Spastic Paraparesis ◽  
Type I ◽  
T Lymphotropic Virus ◽  
Load Measurement

scholarly journals Changes in proviral load (PVL) among patients with rapidly progressive HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) receiving empirical therapy

Retrovirology ◽  
2011 ◽  
Vol 8 (S1) ◽  
Author(s):  
Eduardo Gotuzzo ◽  
Carolina Alvarez ◽  
Elsa González ◽  
Martin A Tipismana ◽  
Giovanni M López ◽  
...  
Keyword(s):  
Proviral Load ◽  
Spastic Paraparesis ◽  
Empirical Therapy ◽  
Tropical Spastic Paraparesis

Relationship among the potato rot nematode, Ditylenchus destructor, densities in soil, root and garlic (Allium sativum) bulbs, and rot damage in stored garlic bulbs

Nematology ◽  
2019 ◽  
Vol 21 (5) ◽  
pp. 547-555 ◽  
Author(s):  
Zejun Cheng ◽  
Koki Toyota ◽  
Rie Aoyama
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Significant Positive Correlation ◽  
Allium Sativum ◽  
Outer Skin ◽  
Positive Correlation ◽  
The Real ◽  
Pcr Method ◽  
Ditylenchus Destructor

Summary The potato rot nematode, Ditylenchus destructor, threatens garlic production in Japan. The objectives of this study were to determine the relationships of D. destructor densities in soil, garlic roots and outer skins of garlic bulbs, and damage to bulbs that rot during storage. Ditylenchus destructor densities were evaluated with the real-time PCR method. There was a significant positive correlation between D. destructor densities in soil at planting and those in the outer skin of garlic bulbs at harvest in 2016, but not in 2017. Ditylenchus destructor densities in outer skins at harvest were consistently low when those in roots at harvest were lower than 80 ind. (0.05 g)−1. No damage to garlic bulbs after storage was observed when D. destructor densities in outer skins were lower than 300 ind. (0.05 g)−1. These results indicate that D. destructor densities in roots and outer skins may be a good indicator to estimate nematode damage to garlic bulbs after storage.

Significance of HTLV-1 proviral load quantification by real-time PCR as a surrogate marker for HTLV-1-infected cell count

2003 ◽  
Vol 25 (2) ◽  
pp. 111-117 ◽  
Author(s):  
S. Kamihira ◽  
N. Dateki ◽  
K. Sugahara ◽  
T. Hayashi ◽  
H. Harasawa ◽  
...  
Keyword(s):  
Infected Cell ◽  
Cell Count ◽  
Real Time ◽  
Real Time Pcr ◽  
Proviral Load ◽  
Surrogate Marker

Real-Time Polymerase Chain Reaction Detection of Asymptomatic Clostridium difficile Colonization and Rising C. difficile–Associated Disease Rates

2014 ◽  
Vol 35 (6) ◽  
pp. 667-673 ◽  
Author(s):  
Hoonmo L. Koo ◽  
John N. Van ◽  
Meina Zhao ◽  
Xunyan Ye ◽  
Paula A. Revell ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Clostridium Difficile ◽  
Real Time ◽  
Real Time Pcr ◽  
Pcr Detection ◽  
Adult Patients ◽  
Incidence Density ◽  
Enzyme Linked Immunosorbent Assay ◽  
Chain Reaction ◽  
Polymerase Chain

Objective.To evaluate the accuracy of real-time polymerase chain reaction (PCR) for Clostridium difficile–associated disease (CDAD) detection, after hospital CDAD rates significantly increased following real-time PCR initiation for CDAD diagnosis.Design.Hospital-wide surveillance study following examination of CDAD incidence density rates by interrupted time series design.Setting.Large university-based hospital.Participants.Hospitalized adult patients.Methods.CDAD rates were compared before and after real-time PCR implementation in a university hospital and in the absence of physician and infection control practice changes. After real-time PCR introduction, all hospitalized adult patients were screened for C. difficile by testing a fecal specimen by real-time PCR, toxin enzyme-linked immunosorbent assay, and toxigenic culture.Results.CDAD hospital rates significantly increased after changing from cell culture cytotoxicity assay to a real-time PCR assay. One hundred ninety-nine hospitalized subjects were enrolled, and 101 fecal specimens were collected. C. difficile was detected in 18 subjects (18%), including 5 subjects (28%) with either definite or probable CDAD and 13 patients (72%) with asymptomatic C. difficile colonization.Conclusions.The majority of healthcare-associated diarrhea is not attributable to CDAD, and the prevalence of asymptomatic C. difficile colonization exceeds CDAD rates in healthcare facilities. PCR detection of asymptomatic C. difficile colonization among patients with non-CDAD diarrhea may be contributing to rising CDAD rates and a significant number of CDAD false positives. PCR may be useful for CDAD screening, but further study is needed to guide interpretation of PCR detection of C. difficile and the value of confirmatory tests. A gold standard CDAD diagnostic assay is needed.Infect Control Hosp Epidemiol 2014;35(6):667–673

scholarly journals Low Annexin A1 level in HTLV-1 Infected Patients might be a Potential Biomarker for the Clinical Progression and Diagnosis of HAM/TSP

2020 ◽  
Author(s):  
Bárbara Santana ◽  
Maria Alice Queiroz ◽  
Rodrigo Cerveira ◽  
Claudia Rodrigues ◽  
Ednelza Amoras ◽  
...  
Keyword(s):  
Gene Expression ◽  
Neurodegenerative Disease ◽  
Proviral Load ◽  
Spastic Paraparesis ◽  
Formyl Peptide Receptor ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Annexin A1 ◽  
Real Time Quantitative Pcr ◽  
Potential Biomarker

Abstract Background: Human T-lymphotropic virus 1 (HTLV-1) is etiologically associated with the chronic inflammatory neurodegenerative disease HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) Annexin A1 (AnxA1) is an anti-inflammatory protein with proposed neuroprotective and anti-neuroinflammatory functions. We hypothesized that ANXA1 gene expression may be dysregulated in HTLV-1-infected HAM/TSP patients.Methods: This study involved 37 individuals infected with HTLV-1, including 21 asymptomatic (AS) carriers and 16 with HAM/TSP, and a control group of 30 individuals negative for HTLV-1 and HTLV-2. For AS HTLV-1-positive and HAM/TSP patients, ANXA1 and formyl peptide receptor (FPR1, FPR2 and FPR3) expression and HTLV-1 proviral load (PVL) in peripheral blood cells were evaluated by real-time quantitative PCR (qPCR), and plasma AnxA1 levels were determined by enzyme-linked immunosorbent assay (ELISA). Results: ANXA1 gene expression was increased in the AS group compared with the HAM/TSP and control groups, but the differences were not statistically significant. FPR1 gene expression was higher in patients with HTLV-1 than in controls (AS, p= 0.0032; HAM/TSP, p< 0.0001). Plasma AnxA1 levels were higher in the AS group than in the HAM/TSP group (p= 0.0045), and PVL was higher in patients with HAM/TSP than in AS individuals (p= 0.0162). The use of a combined ROC curve using Annexin 1 levels and proviral load significantly increased the sensitivity and specificity to predict progression to HAM/TSP (AUC = 0.851 and AUC = 0.937, respectively, to AUC=1,000). Conclusions: Our results suggest that AnxA1 may be dysregulated in HAM/TSP patients. Serological detection of AnxA1 in association with proviral load may provide a prognostic biomarker for HTLV-1-associated neurodegenerative disease.

scholarly journals Prevalence of Bovine viral diarrhea virus in cattle herds from Basrah and Nassirya Provinces by direct and indirect Elisa and Real time qPCR

2012 ◽  
Vol 11 (2) ◽  
pp. 1
Author(s):  
B. A. Jarullah, J. Aed Gati, and A. Saleh
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Indirect Elisa ◽  
Positive Sample ◽  
Enzyme Linked Immunosorbent Assay ◽  
Rt Pcr ◽  
Diarrhea Virus ◽  
Virus Rna ◽  
Two Samples ◽  
Cattle Herds

The current study was conducted to investigate the prevalence of BVD virus in Basrah and Nassirya city by using ELISA and RT-PCR. Two hundreds and eighty two samples of non vaccinated cattle sera samples collected from two regions of Iraq (188 samples from Nassirya city and 92 samples from Basrah city). Samples tested by Enzyme Linked Immunosorbent Assay (ELISA) antigen capture. Positive results were 20 samples ( 8 sample in Thi-Qar and 12 positive samples from Basrah). All samples submitted to indirect ELISA(IDEXX HerdCheck ELISA )for detect BVDV antibodies .Genotyping of all 20 positive samples to antigen detection were tested by Real time PCR, using Cador BVDV ½ kit, after extraction of virus RNA by QIAamp mini kit. The results revealed that there were 20 positive sample according to direct ELISA(Ag detection), while 66 sample were positive to indirect ELISA, as well as, the result of RT-PCR showed that there were two sample positive to BVDV type-1 (one sample form each city).Key words: BVDV, Genotype, ELISA, Iraq, Real time PCR.

scholarly journals HTLV-1 proviral load in infective dermatitis associated with HTLV-1 does not increase after the development of HTLV-1-associated myelopathy/tropical spastic paraparesis and does not decrease after IDH remission

2019 ◽  
Vol 13 (12) ◽  
pp. e0007705 ◽  
Author(s):  
Everton S. Batista ◽  
Pedro D. Oliveira ◽  
Janeusa Primo ◽  
Cinthya Maria Neves Varandas ◽  
Ana Paula Nunes ◽  
...  
Keyword(s):  
Proviral Load ◽  
Spastic Paraparesis ◽  
Tropical Spastic Paraparesis

Use of synthetic oligonucleotides for determination of HTLV‐1 proviral load by real‐time PCR: a helpful alternative approach in the clinical management

2020 ◽  
Vol 129 (3) ◽  
pp. 768-774
Author(s):  
L.M. Bandeira ◽  
M.A.M. Puga ◽  
V.S. Paula ◽  
L.H.F. Demarchi ◽  
G.G.C. Lichs ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Clinical Management ◽  
Proviral Load ◽  
Alternative Approach ◽  
Synthetic Oligonucleotides
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