Influence of location, weather condition, maturity, and plant disease on chemical profiles of dragon fruit (Hylocereus spp.) branches grown in Vietnam

Author(s):  
Nguyen Ngoc Thanh Tien ◽  
Ngoc Lieu Le ◽  
Tran Tien Khoi ◽  
Aurore Richel
2018 ◽  
Vol 7 (1) ◽  
pp. 19
Author(s):  
Herlyan Prasetiyo ◽  
Purwati Purwati ◽  
Iin Arsensi

Utilization of Trichoderma sp fungi as pathogenic fungi antagonists in red dragon fruit plants (Hylocereus polyrhizus) in vitro. The purpose of this study was to identify foul pathogens of dragon fruit plants and then test the ability of Trichoderma sp antagonists to deciduous pathogens of red dragon fruit plants in vitro. The study was conducted from August to October 2016, The research was conducted at the Laboratory of Pest and Plant Disease Sciences, Faculty of Agriculture, Mulawarman University, Samarinda. Sampling of plants exposed to foul tendrils was carried out in Bukit Merdeka Village, Samboja District, Kutai Kartanegara Regency. There are two data observed in this study, primary data and secondary data. Primary data is data obtained directly from the source through direct field observations and laboratory observations and secondary data data obtained from interviews with farmers. The results showed that the pathogen that causes tendon rot in dragon fruit plants is the fungus Colletotrichum gloesporioides (penz. Ssaac). Trichoderma sp can inhibit the development of pathogens Colletotrichum gloesporioides (Penz.) Ssaac., With the highest average resistance of 71.85%. 


2019 ◽  
Vol 1 (1) ◽  
pp. 53
Author(s):  
Nur Adi

This study aims to determine the intensity of stem rot disease in dragon fruit plants in Samboja District Kutai Kartanegara Regency. This research was condu cted from November to December 2017 which is represented by four land i.e. two land located in Sungai Merdeka Village and two land located in Kelurahan Karya Merdeka. The method used in this research was interview and direct observation (survey). The sample was determined by purposive sampling or judgemental sampling method, that is method that chooses subject based on specified specific criteria (symptomatic plants) and sample of dragon fruits tems that attack disease in the field. The sample then isolated and identified the pathogen causing the disease at the Pest and Plant Disease Laboratory, Faculty of Agriculture Mulawarman University. The results of this study indicated that the percentage of stem rot disease in the District of Samboja KutaiKartanegara Regency of 60.83% with the severity of disease 36.12%. Results from the isolation of the ten drills of dragon fruit plants affected by stem rot disease identified the fungus Fusarium sp., Aspergillus sp. And Trichoderma sp.


Plant Disease ◽  
2021 ◽  
Author(s):  
Luz M. Serrato-Diaz ◽  
Ricardo Goenaga

Dragon fruit or pitahaya (Hylocereus spp.) is a tropical fruit belonging to the Cactaceae. It is native to Central and South America and commercially grown in the United States in southern California, south Florida and Puerto Rico. During a disease survey from April to June 2020, stem canker was observed in greenhouses and commercial orchards located in Mayaguez and San Sebastian, Puerto Rico with an incidence of 80%. Diseased cladodes (stems) of 1 mm2 tissue sections of 23 pitahaya varieties (NOI-13, NOI-14, NOI-16, N97-15, N97-17, N97-18, N97-20, N97-22, American Beauty, Cosmic Charlie, Halley’s comet, Purple Haze, Alice, Bloody Mary, Dark Star, David Bowie, Delight, Makisupa, Red Jaina, Soul Kitchen, Vietnamese Jaina, Neitzel and Lisa) were disinfested with 70% ethanol, rinsed with double distilled water and plated on potato dextrose agar (PDA) amended with 60 mg/L streptomycin. Three isolates (17B-173-T3, 12C-118-T1 and 13B-131-T2) of Neoscytalidium dimidiatum (syn. N. hyalinum) were identified using taxonomic keys (Crous et al., 2006) and sequencing of the internal transcribed spacer (ITS) with primers ITS5 and ITS4 (White et al. 1990) and translation elongation factor 1 alpha (TEF1-α) with primers EF1-728F and EF1-986R (Carbone and Kohn, 1999). Sequences were compared using the BLASTn tool with N. dimidiatum deposited in NCBI GenBank. In PDA, colonies of N. dimidiatum were initially powdery white and turned grayish-black with age. Arthroconidia (n=50) were dark brown, disarticulating, truncate or cylindrical at the base, thick-walled with 0 to 1 septum, averaging 9.1 X 5.5um in length. GenBank accession numbers of N. dimidiatum DNA sequences were MT921260, MT921261 and MT921262 for ITS and MT920898, MT920899 and MT920900 for TEF1-α. Sequences were 99-100% identical with Ex-isotype CBS145.78 accession numbers KF531816 for ITS and KF531795 for TEF1-α. Pathogenicity tests were conducted on 12 healthy dragon fruit plants of 1.5 years old using three non-detached cladodes per plant. Cladodes were inoculated with 5mm mycelial plugs from 8-day-old pure cultures grown on PDA. Three healthy dragon fruit plants were used as controls and were inoculated with PDA plugs only. The experiment was repeated once. Twenty days after inoculations (DAI), isolates of N. dimidiatum caused stem canker on dragon fruit plants. For all isolates, sunken orange spots averaged 3 X 2 mm in length at 8 DAI. Necrotic blotches with chlorotic halos averaged 10 X 15 mm at 14 DAI; stem cankers with water-soaked tissue were observed at 20 DAI, and arthroconidia and black pycnidia on dry stem cankers at 30 DAI. Untreated controls had no symptoms of stem canker, and no fungi were isolated from tissue. Neoscytalidium dimidiatum has been reported to cause stem canker on Hylocereus spp. in China, Florida, Israel, Malaysia and Taiwan (Chuang et al. 2012; Lan et al., 2012; Ezra et al., 2013; Sanahuja et al., 2016). To our knowledge, this is the first report of N. dimidiatum causing stem canker on dragon fruit in Puerto Rico. References: 1. Carbone, I., and Kohn, L. 1999. Mycologia, 91:553. doi:10.2307/3761358 2. Chuang, M. F. et al. 2012. Plant Disease 96: 906. https://doi.org/10.1094/PDIS-08-11-0689-PDN. 3. Crous, P. W., et al. 2006. Stud. Mycol. 55:235. https://doi.org/10.3114/sim.55.1.235 4. Ezra et al. 2013. Plant Disease 97: 1513. https://doi.org/10.1094/PDIS-05-13-0535-PDN 5. Lan, G.B. et al. 2012. Plant Disease 96: 1702. https://doi.org/10.1094/PDIS-07-12-0632-PDN 6. Sanahuja et al. 2016. Plant Disease 100: 1499. https://doi.org/10.1094/PDIS-11-15-1319-PDN 7. White, T., Bruns, T., Lee, S., and Taylor, J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. Pages 315-322 in: PCR Protocols: A Guide to Methods and Applications. Academic Press, San Diego, CA.


Author(s):  
Karen K. Baker ◽  
David L. Roberts

Plant disease diagnosis is most often accomplished by examination of symptoms and observation or isolation of causal organisms. Occasionally, diseases of unknown etiology occur and are difficult or impossible to accurately diagnose by the usual means. In 1980, such a disease was observed on Agrostis palustris Huds. c.v. Toronto (creeping bentgrass) putting greens at the Butler National Golf Course in Oak Brook, IL.The wilting symptoms of the disease and the irregular nature of its spread through affected areas suggested that an infectious agent was involved. However, normal isolation procedures did not yield any organism known to infect turf grass. TEM was employed in order to aid in the possible diagnosis of the disease.Crown, root and leaf tissue of both infected and symptomless plants were fixed in cold 5% glutaraldehyde in 0.1 M phosphate buffer, post-fixed in buffered 1% osmium tetroxide, dehydrated in ethanol and embedded in a 1:1 mixture of Spurrs and epon-araldite epoxy resins.


2020 ◽  
Vol 2 (1) ◽  
Author(s):  
Silaturahmi Silaturahmi ◽  
Zaidiyah Zaidiyah ◽  
Tengku Mia Rahmiati

The use of colorants in the dried noodle manufactures is an effort of product diversification. Besides of being used as a natural coloring agent, this peel extract is also used to improve nutritional value of the expected product. The purpose of this study was to determine the effect of red dragon fruit peel extract on the physical quality of dried noodle.  The study was conducted by using Completely Randomized Design (CRD) method with one factor, namely concentration of red dragon fruit peel extract (N1 = 10 ml, N2 = 15 ml, N3 = 20 ml, N4 = 25 ml, N5 = 30 ml).  The observations of its physical quality consisted of water absorption, solid loss during cooking, and organoleptic tests (aroma and color).  The best quality of dried noodle was obtained by using 30 ml red dragon fruit peel extract (N5) with physical properties, namely DSA levels of 351.92%, solid loss during cooking 4.78%, aroma 3.79 (like) and color 3.89 (like).


2018 ◽  
Vol 1 (01) ◽  
pp. 18-21
Author(s):  
Yulian Syahputri ◽  
Diana Widiastuti

Dragon fruit skin, waste material (dragon fruit waste), will have a profitable sale value if it can be used as a food raw material. Dragon fruit skin waste contains relatively high dietary fiber so it can be used as a food raw material. This study aims to utilize dragon fruit skin waste for the manufacture of dragon fruit skin flour as an alternative food source. Some tests are made on white-meat dragon fruit skin, red-meat dragon fruit skin and super red dragon fruit skin. The preliminary study is the soaking of the three types of dragon fruit skin in two solutions, namely 0.1% sodium citrate and 0.1% sodium metabisulfite to prevent the browning effect on flour. Dragon fruit skin flour from the soaking with both solutions is then characterized physically, including its texture, color, flavor and rendement. The best physical characterization is followed by chemical characterization, including the contents of water, ash, protein, fat, carbohydrate, dietary fiber, minerals (Fe, Na, K, Ca and P), and also microbiological characterization of Escherichia coli, molds and Bacillus cereus. The chemical and microbiological characterization shows that the red-meat dragon fruit skin flour has better results than the white-meat and super red dragon fruit skin flour does. The red-meat dragon fruit skin flour contains 8.80% water, 0.20% ash, 2.35% fat, 7.69%, protein, 68,29% carbohydrate and 28,72% dietary fiber as well as 4.40 mg K, 8.76 mg Na , 0.65 mg Fe , 10.20 mg Ca and 32.58 mg P. Keywords: Waste, Dragon Fruit Skin, Dragon Fruit Skin Powder, Alternative Food Source


2018 ◽  
Vol 4 (1) ◽  
pp. 165
Author(s):  
Herry Prabowo ◽  
Mochamad Hilmy

The assessment of the service life of concrete structures using the durability design approach is widely accepted nowadays. It is really encouraged that a simulation model can resemble the real performance of concrete during the service life. This paper investigates the concrete carbonation through probabilistic analysis. Data regarding Indonesian construction practice were taken from Indonesian National Standard (SNI). Meanwhile, data related to Indonesian weather condition for instance humidity and temperature are taken from local Meteorological, Climatological, and Geophysical Agency from 2004 until 2016. Hopefully the results can be a starting point for durability of concrete research in Indonesia.


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