scholarly journals Intensity of Stem Rot Disease in Dragon Fruit (Hylocereus polyrhizus) at Samboja Regency

2019 ◽  
Vol 1 (1) ◽  
pp. 53
Author(s):  
Nur Adi

This study aims to determine the intensity of stem rot disease in dragon fruit plants in Samboja District Kutai Kartanegara Regency. This research was condu cted from November to December 2017 which is represented by four land i.e. two land located in Sungai Merdeka Village and two land located in Kelurahan Karya Merdeka. The method used in this research was interview and direct observation (survey). The sample was determined by purposive sampling or judgemental sampling method, that is method that chooses subject based on specified specific criteria (symptomatic plants) and sample of dragon fruits tems that attack disease in the field. The sample then isolated and identified the pathogen causing the disease at the Pest and Plant Disease Laboratory, Faculty of Agriculture Mulawarman University. The results of this study indicated that the percentage of stem rot disease in the District of Samboja KutaiKartanegara Regency of 60.83% with the severity of disease 36.12%. Results from the isolation of the ten drills of dragon fruit plants affected by stem rot disease identified the fungus Fusarium sp., Aspergillus sp. And Trichoderma sp.

2018 ◽  
Vol 7 (1) ◽  
pp. 19
Author(s):  
Herlyan Prasetiyo ◽  
Purwati Purwati ◽  
Iin Arsensi

Utilization of Trichoderma sp fungi as pathogenic fungi antagonists in red dragon fruit plants (Hylocereus polyrhizus) in vitro. The purpose of this study was to identify foul pathogens of dragon fruit plants and then test the ability of Trichoderma sp antagonists to deciduous pathogens of red dragon fruit plants in vitro. The study was conducted from August to October 2016, The research was conducted at the Laboratory of Pest and Plant Disease Sciences, Faculty of Agriculture, Mulawarman University, Samarinda. Sampling of plants exposed to foul tendrils was carried out in Bukit Merdeka Village, Samboja District, Kutai Kartanegara Regency. There are two data observed in this study, primary data and secondary data. Primary data is data obtained directly from the source through direct field observations and laboratory observations and secondary data data obtained from interviews with farmers. The results showed that the pathogen that causes tendon rot in dragon fruit plants is the fungus Colletotrichum gloesporioides (penz. Ssaac). Trichoderma sp can inhibit the development of pathogens Colletotrichum gloesporioides (Penz.) Ssaac., With the highest average resistance of 71.85%. 


2014 ◽  
Vol 23 (6) ◽  
pp. 3-9
Author(s):  
René Dionisio Cupull-Santana ◽  
Remigio Cortés-Rodríguez ◽  
Ervelio Eliseo Olazábal-Manso ◽  
Carlos Alberto Hernández Medina

La investigación se realizó con el objetivo de evaluar las características fisicoquímicas y la actividad antifúngica de propóleos de Apis mellifera, provenientes de las provincias de Villa Clara, Cienfuegos y Sancti Spíritus, Cuba. Se utilizó el método de recolección por raspado y se estableció el contenido de cera, ceniza, material insoluble y resina de los propóleos crudos. Además, se evaluó la actividad antimicrobiana in vitro frente a los hongos Aspergillus sp., Penicillium sp., Fusarium sp., Trichoderma sp., Colletotrichum sp. y Monilia sp.; los extractos presentaron alta actividad antifúngica en un amplio rango de concentraciones; además, los parámetros fisicoquímicos presentaron valores semejantes y dentro de los establecidos por regulaciones internacionales.


AgriPeat ◽  
2019 ◽  
Vol 20 (02) ◽  
pp. 92-98
Author(s):  
Admin Journal

ABSTRACTThis study aims to determine effective management patterns to control Sclerotium rot and to improvepeat soil fertility with the application of the Trichoderma sp + Aspergillus sp consortium.as biologicalagents and P solvents, combined with spacing arrangements in the cultivation of scallion in peatlands.The study used a factorial randomized block design consisting of two factors with four replications.Factor I Application of biological agents consists of: A0 = No biological agents; A1 = Consortium ofTrichoderma sp. + Aspergillus sp; Factor II Plant spacing, consisting of J1 = 20x25 cm; J2 = 25x25cm and J3 = 30x25 cm. The results showed that the interaction treatment of Trichoderma sp. +Aspergillus sp. and spacing of 25x25 cm effectively suppresses the incidence of white rot disease(Sclerotium cepivorum Berk) up to 34.02%, while at a spacing of 20x25 cm with the application ofTrichoderma sp. + Aspergillus sp. produced the highest fresh plant weight of 8.80 kg plot-1 or 24.44tons hectares-1. Increasing the number of leaves is only influenced by a single factor of biologicalagents (23.29%) and spacing of 25x25 cm (19.7%). Application of Trichoderma sp. + Aspergillus sp.can increase the nutrient content of N, P (total and available), K and peat soil organic matter.Consortium of biological agents Trichoderma sp. and Aspergillus sp. indigenous have the potential tobe developed as biological agents and biofertilizers, with optimum spacing can be applied to themanagement of scallion cultivation in peatlands.Key words: Sclerotium cepivorum Berk, scallion, Trichoderma sp. and Aspergillus sp., spacing


2007 ◽  
Vol 40 (3) ◽  
pp. 326-331 ◽  
Author(s):  
Samuel Dutra Varo ◽  
Carlos Henrique Gomes Martins ◽  
Miguel Jorge de Oliveira Cardoso ◽  
Flávio Garcia Sartori ◽  
Lílian Bueno Montanari ◽  
...  

A despeito da relativa freqüência de infecções fúngicas oportunísticas em pacientes sob hemodiálise, os reservatórios ambientais destes permanecem desconhecidos, embora alguns estudos recentes tenham correlacionado o suprimento de água como fonte desses microrganismos. O objetivo deste trabalho foi monitorar a qualidade micológica do sistema hídrico de uma Unidade de Hemodiálise, do interior do Estado de São Paulo, Brasil, no período entre abril e julho de 2006. Foram coletadas amostras (15), de 1000mL em 7 pontos de distribuição de água empregando-se técnica da membrana filtrante (0,45µm). Foram isolados 116 fungos filamentosos, dos quais 47 (40,5%) Trichoderma sp, 29 (25%) Cladosporium sp, 16 (13,8%) Aspergillus sp e 11 (9,5%) Fusarium sp. Mediante os resultados, sugerimos que suprimentos de água para Unidades de Hemodiálise devam ser monitorados também quanto ao aspecto micológico, adotando-se medidas profiláticas eficazes que minimizem a exposição destes pacientes imunodeficientes a fontes aquáticas ambientais contaminadas.


2014 ◽  
Vol 2 (3) ◽  
Author(s):  
Reny Destya Angrum ◽  
Muhammad Nurdin ◽  
Suskandini Ratih ◽  
Hasriadi Mat Akin

Penelitian ini bertujuan untuk menginventarisasi jamur yang terdapat pada beberapa varietas benih kedelai [ Glycine max (L.) Merril ] yang berada di sekitar Bandar Lampung. Penelitian ini dilakukan di Laboratorium Penyakit Tanaman, UniversitasLampung dari Mei 2013 sampai dengan Agustus 2013. Penelitian ini menggunakan kedelai varieta Burangrang, Kaba, Argomulyo, Tanggamus dan Grobogan. Benih-benih tersebut diinkubasi menggunakan metode blotter test dan agar plate test dengan dua perlakuan, yaitu kontrol (tanpa NaOCl) dan dengan menggunakan NaOCl. Jamur-jamur yang tumbuh dari benih yang diinkubasi diidentifikasi dengan cara diamati dan difoto, selanjutnya dicocokkan dengan bubu-buku referensi tentang penyakit- penyakit kedelai. Selain itu juga dilakukan penghitungan persentase kemunculan jamur dari benih yang diinkubasi. Hasil yang diperoleh dari penelitian yang dilakukan dengan perlakuan desinfeksi maupun tanpa desinfeksi ditemukan jenis jamur yang berasal dari lapang (Fusarium sp., Cercospora sp., Sclerotium sp., Phomopsis sp., Rhizoctonia sp., dan Colletotrichum sp.) dan jenis jamur yang berasal dari gudang penyimpanan (Trichoderma sp., Aspergillus sp., Penicillium sp., dan Rhizopus sp.). Benih yang diberikan perlakuan desinfeksi dapat mengurangi tumbuhnya jamur yang berasal dari gudang/tempat penyimpanan (kontaminan) tetapi tidak menghilangkan jenis jamur yang menginfeksi di dalam benih (jamur yang berasal dari lapang). Pada varietas Grobogan Rhizoctonia sp. memiliki persentase kemunculan jamur yang lebih tinggi dibandingkan dengan jamur lainnya yaitu sebesar 60,56%. Pada benih dengan perlakuan tanpa desinfeksi persentase kemunculan Aspergillus sp. pada varietas Kaba dan Tanggamus lebih tinggi dibandingkan dengan jamur lainnya sebesar 63,33%. Pada media perlakuan desinfeksi dengan menggunakan media kertas saring persentase kemunculan jamur pada varietas Kaba Cercospora sp. memiliki persentase lebih tinggi dibandingkan dengan jamur lainnya sebesar 30%. Pada media perlakuan tanpa desinfeksidengan menggunakan media kertas saring, Trichoderma sp. pada varietas Kaba memiliki persentase kemunculan jamur lebih tinggi dibandingkan dengan jamur lainnya, dengan persentase kemunculan masing-masing sebesar 77,23%.


2019 ◽  
Vol 8 (2) ◽  
pp. 157-167
Author(s):  
Ika Ayuningtyas ◽  
Edi Wibowo Kushartono ◽  
Sri Redjeki

Penyu merupakan vertebrata laut yang termasuk kedalam kategori Appendix 1 yang artinya dilarang diperdagangkan secara internasional. Selain itu penyu salah satu hewan yang paling terancam populasinya secara langsung maupun tidak langsung. Kematian tukik penyu disebabkan oleh berbagai faktor, diantaranya kerusakan habitat alami, pencemaran laut, serangan predator dan perburuan daging maupun telurnya untuk kepentingan komersial. Selain itu, menurunnya jumlah populasi penyu juga disebabkan dari infeksi jamur. Adanya jamur juga menjadi masalah di TCEC (Turtle Conservation and Education Center) yang menyebabkan tingginya tingkat kematian tukik dari penyu lekang (Lepidochelys olivacea). Maka dari itu dengan mengetahui jenis jamur yang ditemukan dapat dilakukan pengobatan serta pencegahan sesuai jamur yang menginfeksi. Tujuan dari penelitian ini adalah untuk mengidentifikasi jamur yang ditemukan pada bagian tubuh penyu lekang (L. olivacea) dan cangkang telur penyu gagal menetas. Sampel tukik dari penyu lekang berumur 10 – 14 hari didapatkan dari penangkaran penyu TCEC Bali. Sampel yang sudah diambil kemudian diswab pada media PDA dan diinkubasi selama 7 hari. Setelah itu dilakukan purifikasi hingga didapatkan koloni murni. Tahap selanjutnya yaitu dilakukannya identifikasi genus secara makroskopis dan mikroskopis pada perbesaran 40x dan 100x. Hasil penelitian memperlihatkan bahwa dari 10 sampel jamur yang diambil dari beberapa bagian tubuh penyu seperti pada mata, kaki, leher dan karapas dan cangkang telur gagal menetas saat sampling, didapatkan 15 isolat koloni jamur. Dari 15 isolat tersebut, ditemukan 5 genus Aspergillus sp, 5 genus Fusarium sp, 3 genus Pytophthora sp dan 1 isolat Trichoderma sp. Pada bagian tubuh jamur yang paling banyak ditemukan yaitu pada bagian leher  sebanyak 4 koloni dan masing-masing jenis jamur bisa ditemukan pada bagian leher. Jenis jamur yang paling banyak ditemukan pada cangkang telur penyu lekang yang gagal menetas yaitu jamur Aspergillus sp yang masing – masing memiliki bercak kuning pada cangkang telur. Selain dari cangkang telur yang memiliki bercak kuning didapatkan juga dari cangkang telur yang melunak.  Turtle is a marine vertebrata that belongs to Appendix 1 category which means it is prohibited to be traded internationally. In addition there is a sea turtle one of animals that are most under threat its population is set directly or indirectly. The death of hatchlings sea turtle was caused by various factors, including natural habitat destruction, marine pollution, predatory attacks and the hunting of meat and eggs for commercial purposes. In addition, the declining number of turtle populations is also caused by fungal infections. The presence of fungi is also a problem in TCEC which causes high mortality rates of turtles (Lepidochelys olivacea). Therefore, by knowing the type of fungus that can be found to be treated as well as prevention of appropriate fungi that infect. The purpose of this study was to identify the fungus found in the turtle body part (L. olivacea) and the turtle eggshell failed to hatch. The turtle samples from turtles aged 10-14 days were obtained from TCEC Bali turtle breeding. Samples were taken later in swabbing on PDA media and incubated for 7 days. After that purification to get a pure colony. The next stage is the identification of the genus in macroscopic and microscopic at 40x and 100x magnification. The results showed that from 10 samples of fungus taken from some parts of sea turtle body such as on eyes, feet, neck and carapace, and eggshell failed to hatch during sampling, 15 isolates of fungal colonies were found. Of the 15 isolates, found 5 genera of Aspergillus sp, 5 genus of Fusarium sp, 3 genus of Pytophthora sp and 1 isolate of Trichoderma sp. In the body of the fungus is most commonly found in the neck as much as 4 colonies and each type of fungus can be found on the neck. The most common types of fungus found in eggshell shells that fail to hatch are the Aspergillus sp fungus, each of which has yellow spots on the eggshell. Apart from the eggshell that has yellow spots it is also obtained from the softened eggshell.


2012 ◽  
Vol 17 (1) ◽  
pp. 61
Author(s):  
Soni Isnaini ◽  
Ainin Niswati ◽  
. Maryati

<p>The experiment was conducted to screen potentials indigenous fungi for rapid decomposing of rice straw.&nbsp; Seven isolates of dominant fungi were isolated from the burying rice straw on the 2.5 cm soil depth after 30 days incubation on the paddy fields. Five dominant isolates were tested for their potential to decompose rice straw by assessing their value of decreasing C/N ratio and dry weight of rice straw.&nbsp; Fungal inoculums treatments were arranged in a Completely Randomized Design with four replications.&nbsp; The results showed that the dominant cultivable fungi that isolated from decomposed rice straw were Trichoderma sp., Fusarium sp., Mucor sp., Aspergillus sp., and Penicillium sp.&nbsp; Among the tested fungi, Trichoderma sp. had the biggest ability to decompose rice straw compared to others indigenous fungi. The C/N ratio was reduced to 39.47 from an initial value of 73.33 of control treatment in 10 days of biodegradation process in laboratory scale, thus showing the potential of indigenous Trichoderma sp. for use in large-scale composting of rice straw.</p><p>Keywords: Cultivable, decomposer, indigenous fungi, rice straw</p><p>[<strong>How to Cite</strong>: Isnaini S, A Niswati and Maryati. 2012. Screening of Cultivatable Indigenous Fungi which Responsible for Decomposing of Rice Straw. J Trop Soils 17 (1): 61-66. doi: 10.5400/jts.2012.17.1.61]</p><p>&nbsp;</p><p>[<strong>Permalink/DOI:</strong> <a href="http://dx.doi.org/10.5400/jts.2012.17.1.61" target="_blank">www.dx.doi.org/10.5400/jts.2012.17.1.61</a>]</p>


2017 ◽  
Vol 43 (3) ◽  
pp. 246-247 ◽  
Author(s):  
Ana Lídia Moura do Carmo ◽  
Edson José Mazaratto ◽  
Bárbara Eckstein ◽  
Álvaro Figueredo dos Santos

RESUMO No Brasil, ainda há poucas pesquisas sobre os microrganismos associados às sementes florestais. O objetivo desse trabalho foi detectar fungos associados às sementes de seis espécies florestais nativas: açoita-cavalo (Luehea divaricata), araçá amarelo (Psidium cattleyanum), corticeira (Erythrina falcata), rabo-de-bugio (Lonchocarpus sp.), vassoura-vermelha (Dodonea viscosa) e pau-jacaré (Piptadenia gonoacantha). Foram identificados oito gêneros de fungos potencialmente patogênicos: Cladosporyum sp., Fusarium sp., Pestalotiopsis sp., Phomopsis sp., Coletotrichum sp., Alternaria sp., Phoma sp. e Botrytis sp. e fungos saprófitas: Aspergillus sp., Penicillium sp., Rhizopus sp., Epicoccum sp., Torula sp., Trichoderma sp., Gliocladium sp., Chaetomium sp. e Trichothecium sp. Os resultados evidenciam a diversidade de fungos associados às sementes florestais nativas, incluindo vários potencialmente fitopatogênicos.


2018 ◽  
Vol 2 (1) ◽  
Author(s):  
Iin Arsensi

The pathogen attacks the host plant in various ways to obtain the food substance needed by the host pathogen. To get into the host pathogen is able to break the host's defense reaction. The aim of the study was to know the symptoms and signs of stem rot disease in E. pellita in the nursery. In addition, also to determine the frequency and intensity of rodent pathogen attack in plant seeds E. pellita from shoot cuttings. Research in the nursery begins by determining the sample of plant seeds to be observed. Seed determination is done by census. Seedlings consisted of 120 plants in a plastic rack that was repeated as many as 3 replications so that the total number of seedlings observed was 120 plant seed x 3 replicates x 4 families = 1.440 plant seeds. The pathogen that causes stem rot on E. pellita seed is Fusarium sp which is transmitted through soil.


2016 ◽  
Vol 3 (1) ◽  
pp. 36
Author(s):  
Andri Kurniawan ◽  
Nuraeni Ekowati

Heavy metals contamination is a main issue which has negative impacts to environment and organisms. Various methods have been developed to reduce such pollutants, including utilization of organisms’ capability in order to minimize the contamination. Mycoremediation is one of remediation process in contaminated environment using fungi and its reduction mechanisms, involving intracellular, as well as extracellular system. There are some species of fungi that are frequently used as remediator agents, for example Aspergillus sp., Fusarium sp., Penicillium sp., Phanerochaete sp., Trichoderma sp. There are some methods that have been used for heavy metal reduction mechanisms such as biosorption, bioaccumulation, bioprecipitation, bioreduction, and bioleaching.Keywords: Mycoremediation, fungi, heavy metal, biosorption, bioaccumulation ABSTRAK Kontaminasi logam berat adalah suatu permasalahan utama yang berdampak negatif bagi lingkungan dan juga makhluk hidup. Berbagai metode telah dikembangkan untuk mereduksi cemaran, termasuk memanfaatkan kemampuan organisme untuk meminimalkan kontaminan tersebut. Mikoredemiasi adalah salah satu proses remediasi cemaran di lingkungan dengan melibatkan fungi beserta mekanisme reduksinya, baik secara intraselular maupun ekstraselular. Beberapa jenis fungi yang sering dijadikan agen remediator antara lain Aspergillus sp., Fusarium sp., Penicillium sp., Phanerochaete sp., Trichoderma sp. Beberapa prinsip yang digunakan untuk menghilangkan logam berat antara lain biosorpsi, bioakumulasi, biopresipitasi, bioreduksi, dan bioleaching. Kata kunci: Mikoremediasi, fungi, logam berat, biosorpsi, bioakumulasi


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