Influence of the total concentration and the profile of volatile fatty acids on polyhydroxyalkanoates (PHA) production by mixed microbial cultures

AbstractPolyhydroxyalkanoates (PHAs) production from lignocellulosic biomass using mixed microbial cultures (MMC) is a potential cheap alternative for reducing the use of petroleum-based plastics. In this study, an MMC adapted to acidogenic effluent from dark fermentation (DF) of exhausted sugar beet cossettes (ESBC) has been tested in order to determine its capability to produce PHAs from nine different synthetic mixtures of volatile fatty acids (VFAs). The tests consisted of mixtures of acetic, propionic, butyric, and valeric acids in the range of 1.5–9.0 g/L of total acidity and with three different valeric:butyric ratios (10:1, 1:1, and 1:10). Experimental results have shown a consistent preference of the MMC for the butyric and valeric acids as carbon source instead other shorter acids (propionic or acetic) in terms of PHA production yield (estimated in dry cell weight basis), with a maximum value of 23% w/w. Additionally, valeric-rich mixtures have demonstrated to carry out a fast degradation process but with poor final PHA production compared with high butyric mixtures. Finally, high initial butyric and valeric concentrations (1.1 g/L and 4.1 g/L) have demonstrated to be counterproductive to PHA production.

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Effect of the solid retention time in the obtention of polyhydroxyalkanoates

F1000Research ◽

10.12688/f1000research.28852.1 ◽

2021 ◽

Vol 10 ◽

pp. 864

Author(s):

Rolando Calero ◽

Manuel Martínez

Keyword(s):

Fatty Acids ◽

Retention Time ◽

Volatile Fatty Acids ◽

Batch Reactor ◽

Oxygen Demand ◽

Solid Retention Time ◽

Dry Cell Weight ◽

Acidogenic Reactor ◽

Mixed Microbial Cultures ◽

Dry Cell

Background: The effect of solid retention time (SRT) over cheese whey substrates in a fermentation process drives changes in the composition of polyhydroxyalkanoates (PHAs) obtained. Volatile fatty acids produced in the first step of an anaerobic sequencing batch reactor were used as substrates to produce PHA using mixed microbial cultures under aerobic dynamic feeding conditions. Methods: Analytical methods were used for the standard analysis of parameters of interest including measuring the amount of ammonium and phosphate, chemical oxygen demand, among others. Results: The SRT increasing from 4 to 6 and 10 days produced changes in the distribution of volatile fatty acids produced. The polyhydroxybutyrate-hydroxyvalerate copolymers formed in the accumulation stage gave the following results: 58:42, 68:32 and 81:19 (%), referred to SRTs of 10, 6 and 4 days, respectively. The maximum PHA accumulation obtained at 10 days of SRT was 52% of the dry cell weight within 7 h, reaching a PHA productivity of 0.62 g L−1 h−1 and a storage yield of 0.37. Conclusion: The SRT variation impact on the distribution of volatile fatty acids in the acidogenic reactor and consequently on the PHA production and composition formed in the accumulation stage.

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Metabolic modeling of polyhydroxyalkanoates (PHA) production from complex mixtures of competing volatile fatty acids (VFA) by mixed microbial cultures (MMC)

Journal of Biotechnology ◽

10.1016/j.jbiotec.2010.10.035 ◽

2010 ◽

Vol 150 ◽

pp. 565-565 ◽

Cited By ~ 1

Author(s):

F. Pardelha ◽

M.G.E. Albuquerque ◽

M.A.M. Reis ◽

R. Oliveira ◽

J.M.L. Dias

Keyword(s):

Fatty Acids ◽

Volatile Fatty Acids ◽

Metabolic Modeling ◽

Complex Mixtures ◽

Microbial Cultures ◽

Mixed Microbial Cultures ◽

Pha Production

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Identifikasi Senyawa Asam Lemak Volatil dari Air Limbah Industri Minyak Kelapa Sawit untuk Produksi Polihidroksialkanoat oleh Ralstonia eutropha JMP 134

JURNAL SELULOSA ◽

10.25269/jsel.v11i01.340 ◽

2021 ◽

Vol 11 (01) ◽

pp. 49

Author(s):

Martha Aznury ◽

Tjandra Setiadi

Keyword(s):

Fatty Acids ◽

Palm Oil ◽

Industrial Wastewater ◽

Volatile Fatty Acids ◽

Ralstonia Eutropha ◽

Bacterial Fermentation ◽

Dry Cell Weight ◽

Pha Production ◽

Palm Oil Mill ◽

Dry Cell

Identification of Volatile Fatty Acids from Palm Oil Mill Effluent for Polyhydroxyalkanoate Production by Ralstonia eutropha JMP 134 Abstract Polyhydroxyalkanoate (PHA) is a bioplastic which is derived from bacterial fermentation. In this study, PHA is produced by utilizing Ralstonia eutropha JMP 134 and volatile fatty acids (VFA) from palm oil industrial wastewater as a precursor. The aim of this research is to study the effect of carbon source, addition time, and VFA concentration on PHA production by fermentation using Ralstonia eutropha JMP 134 in batch. PHA and dry cell weight (DCW) concentrations obtained by adding VFA from palm oil industrial wastewater in batches at 20 and 40 hours were 0.014 g/L.hour, 2.76 g/L and 3.66 g/L, respectively. The results also showed that the time of adding VFA greatly affected cell growth, with the best addition time being after the 20th hour. Keywords: palm oil industrial wastewater, polyhydroxyalkanoate (PHA), batch, Ralstonia eutropha JMP 134, volatile fatty acids (VFA) Abstrak Polihidroksialkanoat (PHA) adalah bioplastik yang diproses melalui proses fermentasi dengan mikroba. Pada penelitian ini, PHA diproduksi dengan menggunakan Ralstonia eutropha JMP 134 dan memanfaatkan asam lemak volatil (ALV) dari air limbah industri minyak kelapa sawit sebagai prekursor. Penelitian ditujukan mempelajari pengaruh sumber karbon, waktu penambahan, dan konsentrasi ALV terhadap poduksi PHA yang difermentasi menggunakan Ralstonia eutropha JMP 134 secara batch. Konsentrasi PHA dan berat kering sel (BKS) yang diperoleh pada penambahan ALV dari air limbah industri kelapa sawit secara batch pada jam ke-20 dan 40 masing-masing bernilai 0,014 g/L.jam, 2,76 g/L dan 3,66 g/L. Hasil penelitian memperlihatkan pula bahwa waktu penambahan ALV sangat mempengaruhi pertumbuhan sel, dengan waktu penambahan yang terbaik adalah setelah pada jam ke-20. Kata kunci: air limbah industri minyak kelapa sawit, polihidroksialkanoat (PHA), batch, Ralstonia eutropha JMP 134, asam lemak volatil (ALV)

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Insights into enhanced polyhydroxyalkanoate production by the synergistic use of waste wood hydrolysate and volatile fatty acids by mixed microbial cultures

Bioresource Technology ◽

10.1016/j.biortech.2021.125488 ◽

2021 ◽

pp. 125488

Author(s):

Dongna Li ◽

Xiaojun Ma ◽

Jianing Li ◽

Binqing Sun

Keyword(s):

Fatty Acids ◽

Volatile Fatty Acids ◽

Waste Wood ◽

Microbial Cultures ◽

Mixed Microbial Cultures ◽

Wood Hydrolysate

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From Food Waste to Volatile Fatty Acids towards a Circular Economy

10.5772/intechopen.96542 ◽

2021 ◽

Author(s):

Mónica Carvalheira ◽

Anouk F. Duque

Keyword(s):

Fatty Acids ◽

Food Waste ◽

Circular Economy ◽

Volatile Fatty Acids ◽

Animal Feed ◽

Anaerobic Fermentation ◽

Industrial Sector ◽

Added Value ◽

Microbial Cultures ◽

Mixed Microbial Cultures

The food industrial sector generates large amounts of waste, which are often used for animal feed, for agriculture or landfilled. However, these wastes have a very reach composition in carbon and other compounds, which make them very attractive for valorization through biotechnological processes. Added value compounds, such as volatile fatty acids (VFAs), can be produced by anaerobic fermentation using pure cultures or mixed microbial cultures and food waste as carbon source. Research on valuable applications for VFAs, such as polyhydroxyalkanoates, bioenergy or biological nutrient removal, towards a circular economy is emerging. This enhances the sustainability and the economic value of food waste. This chapter reviews the various types of food waste used for VFAs production using mixed microbial cultures, the anaerobic processes, involved and the main applications for the produced VFAs. The main parameters affecting VFAs production are also discussed.

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Flux balance analysis of mixed microbial cultures: Application to the production of polyhydroxyalkanoates from complex mixtures of volatile fatty acids

Journal of Biotechnology ◽

10.1016/j.jbiotec.2012.08.017 ◽

2012 ◽

Vol 162 (2-3) ◽

pp. 336-345 ◽

Cited By ~ 36

Author(s):

Filipa Pardelha ◽

Maria G.E. Albuquerque ◽

Maria A.M. Reis ◽

João M.L. Dias ◽

Rui Oliveira

Keyword(s):

Fatty Acids ◽

Flux Balance Analysis ◽

Volatile Fatty Acids ◽

Complex Mixtures ◽

Flux Balance ◽

Microbial Cultures ◽

Balance Analysis ◽

Mixed Microbial Cultures

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Bio-based conversion of volatile fatty acids from waste streams to polyhydroxyalkanoates using mixed microbial cultures

Bioresource Technology ◽

10.1016/j.biortech.2020.124604 ◽

2021 ◽

Vol 323 ◽

pp. 124604

Author(s):

Mariel Perez-Zabaleta ◽

Merve Atasoy ◽

Kasra Khatami ◽

Elsa Eriksson ◽

Zeynep Cetecioglu

Keyword(s):

Fatty Acids ◽

Volatile Fatty Acids ◽

Waste Streams ◽

Microbial Cultures ◽

Mixed Microbial Cultures

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Assessment of Long-Term Fermentability of PHA-Based Materials from Pure and Mixed Microbial Cultures for Potential Environmental Applications

Water ◽

10.3390/w13070897 ◽

2021 ◽

Vol 13 (7) ◽

pp. 897

Author(s):

Neda Amanat ◽

Bruna Matturro ◽

Marta Maria Rossi ◽

Francesco Valentino ◽

Marianna Villano ◽

...

Keyword(s):

Environmental Remediation ◽

Volatile Fatty Acids ◽

Rapid Onset ◽

Pilot Scale ◽

Point Of View ◽

Microbial Cultures ◽

Extraction And Purification ◽

Fermentation Pattern ◽

Mixed Microbial Cultures

The use of polyhydroxyalkanoates (PHA) as slow-release electron donors for environmental remediation represents a novel and appealing application that is attracting considerable attention in the scientific community. In this context, here, the fermentation pattern of different types of PHA-based materials has been investigated in batch and continuous-flow experiments. Along with commercially available materials, produced from axenic microbial cultures, PHA produced at pilot scale by mixed microbial cultures (MMC) using waste feedstock have been also tested. As a main finding, a rapid onset of volatile fatty acids (VFA) production was observed with a low-purity MMC-deriving material, consisting of microbial cells containing 56% (on weight basis) of intracellular PHA. Indeed, with this material a sustained, long-term production of organic acids (i.e., acetic, propionic, and butyric acids) was observed. In addition, the obtained yield of conversion into acids (up to 70% gVFA/gPHA) was higher than that obtained with the other tested materials, made of extracted and purified PHA. These results clearly suggest the possibility to directly use the PHA-rich cells deriving from the MMC production process, with no need of extraction and purification procedures, as a sustainable and effective carbon source bringing remarkable advantages from an economic and environmental point of view.

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Rumen volatile fatty acids on six pasture species and relations with digestibility, voluntary intake and chemical composition

Australian Journal of Experimental Agriculture ◽

10.1071/ea9740161 ◽

1974 ◽

Vol 14 (67) ◽

pp. 161 ◽

Cited By ~ 1

Author(s):

PJ Michell

Keyword(s):

Fatty Acids ◽

Lolium Perenne ◽

White Clover ◽

Volatile Fatty Acids ◽

Total Concentration ◽

Dactylis Glomerata ◽

Single Species ◽

Water Soluble ◽

Soluble Carbohydrate ◽

Seasonal Differences

The total concentration and the relative proportions of the volatile fatty acids (VFA) were measured in rumens of sheep fed 78 single species pasture samples. The pastures consisted of regrowths of white clover (Trifolium repens cv. Grasslands Huia), Manawa regrass (Lolium perenne x multiflorum cv. Grasslands Manawa), Ariki ryegrass (Lolium (multiflorum x perenne) x L. perenne cv. Grasslands Ariki), perennial ryegrass (Lolium perenne cv. Tasmania No. 1) , Currie cocksfoot (Dactylis glomerata cv. Currie) and Apanui cocksfoot (Dactylis glomerata cv. Grasslands Apanui), cut eight times between May, 1969 and August, 1970. Sheep fed cocksfoot had lower VFA concentrations, higher proportions of acetate (HAc) and lower proportions of butyrate (HBu) than sheep fed white clover and ryegrass (P < 0.05). They also had lower proportions of propionate (HPr) than sheep fed ryegrass (P < 0.05). Sheep fed white clover had higher proportions of valerate (HVa) than those fed the grasses (P < 0.05). There were no significant (P < 0.05) seasonal differences in total VFA concentration but HAc was lowest with spring pasture (P < 0.05) and HBu was lowest with autumn pasture (P < 0.05). Total VFA concentration was positively related to digestibility (DMD) (r = +0.52, P < 0.01) but species differences occurred and at similar DMD, white clover produced higher concentrations than ryegrass (P < 0.05) which produced higher concentrations than cocksfoot (P < 0.01). The overall relation between total VFA and intake (DMI) was weak but seasonal differences occurred and at similar levels of DMI, winter pasture produced higher VFA concentrations than spring-summer pasture (P < 0.01). The proportion of HPr was positively related to VFA concentration (r = +0.53, P < 0.01) and to water soluble carbohydrate (WSC) content (r = +0.51, P < 0.01) and relations with DMD and DM1 were weak and non-significant (P > 0.05).

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