Postharvest chitosan application maintains the quality of spinach through suppression of bacterial growth and elicitation

Author(s):  
Karlia Meitha ◽  
Yonadita Pramesti ◽  
Santiago Signorelli ◽  
Jayen Aris Kriswantoro
Keyword(s):  
Author(s):  
Nourhan Mahrous Ahmed Salem ◽  
Mohamed Abbas Elbarrawy ◽  
Nashwa Fawzy Abd El Moez Azzam

Abstract Background The context and purpose of the study are as follows: Drug-borne infections may arise from non-adherence to strict microbiological quality of pharmaceuticals products. Moreover, presence of exceeding levels of microorganisms in non-sterile pharmaceuticals may lead to change of their organoleptic characteristics and loss of effectiveness. The aim of the study is to evaluate the microbiological quality of commonly used non-sterile pharmaceuticals in Alexandria, Egypt. Results Average microbiological quality of the studied products, where 17.03% and 19.23 % of samples had exceeded the maximum acceptable limit of TAMC and TYMC, respectively. No E. coli was isolated from oral products. None of S. aureus nor P. aeruginosa were isolated from topical products. Bacterial growth was recovered from 19 (10.44%) of the studied 182 samples, four Bacillus spp. had been recovered from topical products, two P. aeruginosa isolates were recovered from tablets and other two were isolated from syrups dosage forms. Other isolates were Pseudomonas stutzeri, Stenotrophomonas maltophilia, Acinetobacter Achromobacter denitrificans, Ochrobactrum anthropic and Aeromonas salmonicida. Conclusion Average microbiological quality of the tested pharmaceuticals used in Alexandria.


2014 ◽  
Vol 644-650 ◽  
pp. 4671-4676
Author(s):  
Ying Chun Zhu ◽  
Li Zhen Ma ◽  
Yu Jing Tian ◽  
Hua Yang ◽  
Yao Hua Guo ◽  
...  

The objective of this study was to evaluate the use of high pressure processing (HPP) as a preservation method of meat products. Vacuum-packaged fish patties were subjected to HPP (300 MPa for 30 min 15°C or 500 Mpa for 10 min at 15°C). Untreated samples represented the control group. The three groups were stored at 4°C for 0–5 weeks. Color parameters, pH, thiobarbituric (TBARS), bacterial growth, and Oxidation-Reduction Potential (ORP) were determined. The results revealed that the 500-MPa treatment inhibited bacterial growth and extended the shelf-life of fish patties to four weeks with insignificant effects on the physicochemical attributes.


2020 ◽  
Vol 38 (2) ◽  
pp. 126
Author(s):  
Apris Beniawan ◽  
Agustin Indrawati ◽  
Fachriyan Hasmi Pasaribu

Campylobacter fetus subsp. Venerealis (Cfv) is bacteria causing contagious genital diseases in cows called Bovine Genital Campylobacteriosis (BGC) or vibriosis. Isolation of Cfv is difficult, because the bacteria are fragile and need specific nutrients and oxygen (5-10%). The transport media is very important to maintain Cfv survival before culturing in laboratory. The aim of this study was to modify a new transport media as an alternative media for Cfv. Developed media capability was compared to Weybridge media, and Phosphate Buffered Saline (PBS). All transport media was contaminated by Cfv with concentrations of 105,104,103,102,101 (CFU/ml), and was stored for <6, 24, 48, 72, and 96 hours in each transport medium before culturing on blood agar, all in triplicate. The quality of transport media was analyzed based on bacterial growth on blood agar. PCR test was used as a confirmatory test of growing bacteria cultured on blood agar. Based on culture results, Cfv stored in three transport mediums for <6 hours, Cfv grew on blood agar from all concentration levels provided. Cfv stored for 24, 48, 72 and 96 hours on PBS did not grow, whereas on developed media and Weybridge media, the bacteria could grow and enrichment occurs at all concentration levels given. This study indicated the developed media can be used as an alternative transport medium for Cfv bacteria.


2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Yajing Xie ◽  
Qiong Lin ◽  
Wenqiang Guan ◽  
Shuzhen Cheng ◽  
Zhidong Wang ◽  
...  

Fresh-cut vegetables, such as potato chips, get brown quickly and can easily be infected by bacterium during storage. Sodium acid sulfate (SAS) and UV-C treatments are regarded as effective methods for food preservation. In this study, the effects of SAS, UV-C treatment, and their combination on fresh-cut potatoes during storage were evaluated. Compared with the control, all of the treatments were effective in inhibiting the bacterial growth during the whole storage period. Also, both SAS and SAS + UV-C treatments significantly decreased browning and polyphenol oxidase (PPO) activity and increased the firmness and malondialdehyde (MDA) contents, while the UV-C treatment has no good effects on protecting such storage qualities in fresh-cut potatoes. However, when compared with SAS treatment, the combination of SAS and UV-C treatment did not promote the effect in protecting the storage abilities. Thus, it was concluded that SAS is a better treatment in extending shelf life and controlling the quality of fresh-cut potatoes during storage compared to UV-C treatment.


1975 ◽  
Vol 38 (10) ◽  
pp. 570-572 ◽  
Author(s):  
SANDRA L. ANAGNOSTAKIS ◽  
LESTER HANKIN

Media are described for use in food analysis to detect fungi and bacteria able to excrete extracellular lipases, proteases, pectinases, amylases, and acids. Antibiotics were used to suppress bacterial growth in media that detect fungi. Food products were examined to show the ability of these media to detect contaminating microorganisms able to excrete these enzymes. The possibility that keeping quality of foods could be correlated with numbers of organisms able to excrete these degradative enzymes is discussed. The test media also could be used to obtain total counts of fungal and bacterial populations.


2013 ◽  
Vol 79 (6) ◽  
pp. 1020-1022
Author(s):  
TAKAHIRO NAGAI ◽  
MISATO NAKAMORI ◽  
KEN-ICHIRO MIZUNO ◽  
HIDEYUKI TAKATUJI ◽  
MAKOTO WAKANO

1982 ◽  
Vol 49 (3) ◽  
pp. 425-437 ◽  
Author(s):  
Monika J. A. Schröder

SUMMARYThe effect of limiting the amount of O2 present in commercially pasteurized milk on bacterial growth and on the keeping quality of the milk was investigated. No increase in bacterial count of laboratory pasteurized milk during storage for 9 d at 5 °C was observed and O2 consumption due to bacterial activity was low. In commercial pasteurized milk containing post-pasteurization contamination (PPC) growth of strict aerobes was encouraged by high O2 content while that of coliforms was less dependent on O2 level. The amounts of headspace (HS) O2 consumed in milk with PPC growth were large. Reducing the dissolved O2 content of commercial pasteurized milk stored in O2-impermeable containers without HS had little effect on bacterial activity and shelf-life.


1975 ◽  
Vol 75 (1) ◽  
pp. 113-119 ◽  
Author(s):  
Margaret M. Taylor

SUMMARYBacteriological tests alone are not capable of predicting the keeping quality of cream, since taints can develop which are due to non-bacterial action. When spoilage is due to bacterial growth, the water agar test was found to be more accurate than the other tests that were examined.


2017 ◽  
Vol 89 ◽  
pp. 235-243 ◽  
Author(s):  
Eva Bussalleu ◽  
Sílvia Sancho ◽  
Maria D. Briz ◽  
Marc Yeste ◽  
Sergi Bonet

2021 ◽  
Author(s):  
Domenica Mallardi ◽  
Chiara Tabasso ◽  
Pasqua Piemontese ◽  
Stefano Morandi ◽  
Tiziana Silvetti ◽  
...  

Abstract Background Human milk is a vehicle of bioactive compounds and beneficial bacteria which promote the establishment of a healthy gut microbiome of newborns, especially of preterm infants. Pasteurized donor human milk (PDHM) is the second-best option when preterm mother’s own milk is unavailable. Since pasteurization affect the quality of donor milk, the effect on bacterial growth, human milk microbiota diversity and proteolytic phenomena in PDHM inoculated with different preterm milk samples, was evaluated. Methods Ten preterm milk samples was used to perform inoculation of PDHM at 10% v/v. Microbiological, metataxonomic and peptidomic analysis, on preterm milk samples at the baseline (T0) and PDHM and inoculated milk (IM) samples at T0, after 2h (T1) and 4h (T2) of incubation at 37°C, were conducted. Results IM samples at T2 showed a Total Bacterial Count not significantly different (p > 0.01) compared to preterm milk samples. Lactic acid bacteria (LAB) level was restored in all IM at T2. After inoculation, metataxonomic analysis showed that Proteobacteria remained the predominant phylum while Firmicutes moved from 3% at T1 to 9.4% at T2 in IM samples. Peptidomic profile of IM resembled that of PDHM incubated for the same time in terms of number and type of peptides. Conclusion The study demonstrated that inoculation with fresh mother’s own milk could restore bacterial growth and personalize human milk microbiome in PDHM. This effect could be beneficial thanks to the presence of maternal probiotic bacteria which make PDHM more similar to mother’s own milk.


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