A Review on Application of DNA Barcoding Technology for Rapid Molecular Diagnostics of Adulterants in Herbal Medicine

Drug Safety ◽  
2021 ◽  
Author(s):  
Alok Senapati ◽  
Supriyo Basak ◽  
Latha Rangan
Keyword(s):  
Herbal Medicine ◽  
Dna Barcoding ◽  
Molecular Diagnostics ◽  
Barcoding Technology

DNA barcoding for identification of the endangered species Aquilaria sinensis: comparison of data from heated or aged wood samples

Holzforschung ◽  
2014 ◽  
Vol 68 (4) ◽  
pp. 487-494 ◽  
Author(s):  
Lichao Jiao ◽  
Yafang Yin ◽  
Yeming Cheng ◽  
Xiaomei Jiang
Keyword(s):  
Dna Barcoding ◽  
Dna Sequences ◽  
Phylogenetic Trees ◽  
Species Level ◽  
Related Genus ◽  
Illegal Logging ◽  
Aquilaria Sinensis ◽  
Long Period ◽  
High Temperature Drying ◽  
Barcoding Technology

Abstract Aquilaria sinensis (Lour.) Gilg is an evergreen tree and produces agarwood used for incense and as a uniquely precious medicine. It is in danger of disappearing due to illegal logging and its identification and protection is crucial. However, it is difficult or impossible to distinguish A. sinensis from other species of the genus Aquilaria Lam. and its closely related genus Gyrinops Gaertn. based on wood anatomical characteristics. Probably, DNA barcoding technology might provide an improvement in species identification. In this study, wood samples were tested, which were submitted to high-temperature drying and were stored for a long period in a xylarium. The factors should be identified that hinder the efficiency of wood DNA extraction from this species. The results indicate that the DNA from the wood tissues could be successfully amplified, apart from some DNA regions from the heartwood of the dried samples and the xylarium samples. The DNA sequences from the wood tissues mostly matched with the sequences of A. sinensis deposited in the GenBank. Moreover, analyses of phylogenetic trees based on trnL-trnF and ITS1 regions indicated that the wood tissues in the tests clustered together with the A. sinensis species from the GenBank, with bootstrap values of 74% and 94%, respectively. Consequently, it is feasible to identify A. sinensis wood on a species level based on the DNA barcoding technology.

Strategy and Key Technique of Identification of Chinese Herbal Medicine Using DNA Barcoding

2010 ◽  
Vol 7 (5) ◽  
pp. 322-327 ◽  
Author(s):  
Shi-Lin CHEN ◽  
Jing-Yuan SONG ◽  
Hui YAO ◽  
Lin-Chun SHI ◽  
Kun LUO ◽  
...  
Keyword(s):  
Herbal Medicine ◽  
Dna Barcoding ◽  
Chinese Herbal Medicine ◽  
Chinese Herbal

scholarly journals DNA Barcoding for the Identification of Botanicals in Herbal Medicine and Dietary Supplements: Strengths and Limitations

Planta Medica ◽  
2016 ◽  
Vol 82 (14) ◽  
pp. 1225-1235 ◽  
Author(s):  
Iffat Parveen ◽  
Stefan Gafner ◽  
Natascha Techen ◽  
Susan Murch ◽  
Ikhlas Khan
Keyword(s):  
Herbal Medicine ◽  
Dietary Supplements ◽  
Dna Barcoding

scholarly journals Identification of ungulates used in a traditional Chinese medicine with DNA barcoding technology

2015 ◽  
Vol 5 (9) ◽  
pp. 1818-1825 ◽  
Author(s):  
Jing Chen ◽  
Zhigang Jiang ◽  
Chunlin Li ◽  
Xiaoge Ping ◽  
Shaopeng Cui ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Dna Barcoding ◽  
Barcoding Technology

scholarly journals DNA barcoding of medicinal orchids in Asia

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Bhakta Bahadur Raskoti ◽  
Rita Ale
Keyword(s):  
Herbal Medicine ◽  
Dna Barcoding ◽  
Global Markets ◽  
Morphological Observation ◽  
Correct Identification ◽  
Illegal Trade ◽  
Natural Habitats ◽  
Accurate Identification ◽  
Orchid Species ◽  
Important Method

AbstractGrowing popularity of herbal medicine has increased the demand of medicinal orchids in the global markets leading to their overharvesting from natural habitats for illegal trade. To stop such illegal trade, the correct identification of orchid species from their traded products is a foremost requirement. Different species of medicinal orchids are traded as their dried or fresh parts (tubers, pseudobulbs, stems), which look similar to each other making it almost impossible to identify them merely based on morphological observation. To overcome this problem, DNA barcoding could be an important method for accurate identification of medicinal orchids. Therefore, this research evaluated DNA barcoding of medicinal orchids in Asia where illegal trade of medicinal orchids has long existed. Based on genetic distance, similarity-based and tree-based methods with sampling nearly 7,000 sequences from five single barcodes (ITS, ITS2, matK, rbcL, trnH-psbA and their seven combinations), this study revealed that DNA barcoding is effective for identifying medicinal orchids. Among single locus, ITS performed the best barcode, whereas ITS + matK exhibited the most efficient barcode among multi-loci. A barcode library as a resource for identifying medicinal orchids has been established which contains about 7,000 sequences of 380 species (i.e. 90%) of medicinal orchids in Asia.

scholarly journals The plant original identification of Inner Mongolia Arnebia Radix based on morphology and DNA barcoding

2020 ◽  
Author(s):  
Jie Liu ◽  
Chunyan Zhao ◽  
Linong Guo ◽  
Shuangcheng Ma ◽  
Jian Zheng ◽  
...  
Keyword(s):  
Herbal Medicine ◽  
Dna Barcoding ◽  
Inner Mongolia ◽  
Herbal Medicines ◽  
Morphological Characteristics ◽  
Plant Origin ◽  
Original Plant

Abstract Background: It’s a long history for the use of herbal medicines to derive remedies in Chinese, and the popularity of herbal medicines has risen worldwide. It is necessary to assure the safety, efficacy and quality of herbal medicines by the corrected identification. Inner Mongolia Arnebia Radix is an important and historied used herbal medicine in Inner Mongolia.Methods: The methods of morphological characteristics and DNA barcoding was used to identify the plant origin of Inner Mongolia Arnebia Radix.Results: The identification results of morphological characteristics and DNA barcoding showed that the original plant of Inner Mongolia Arnebia Radix was Arnebia szechenyi, which was distinguished with Arnebia guttata, the official original plant of Inner Mongolia Arnebia Radix.Conclusions: We inferred that that the original plant of Inner Mongolia Arnebia Radix was Arnebia szechenyi.

DNA barcoding technology to identity spawn from the Jiangmen marine environment in summer

2018 ◽  
Vol 25 (4) ◽  
pp. 721
Author(s):  
Nan ZHANG ◽  
Na WU ◽  
Huayang GUO ◽  
Kecheng ZHU ◽  
Yong LIU ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
Marine Environment ◽  
Barcoding Technology

scholarly journals An economic analysis of private incentives to adopt DNA barcoding technology for fish species authentication in Canada

Genome ◽  
2015 ◽  
Vol 58 (12) ◽  
pp. 559-567 ◽  
Author(s):  
Albert I. Ugochukwu ◽  
Jill E. Hobbs ◽  
Peter W.B. Phillips ◽  
Richard Gray
Keyword(s):  
Dna Barcoding ◽  
Fish Species ◽  
Retail Store ◽  
Third Party ◽  
Economic Gain ◽  
The Public ◽  
Information Problem ◽  
Species Substitution ◽  
Private Incentives ◽  
Barcoding Technology

The increasing spate of species substitution and mislabelling in fish markets has become a concern to the public and a challenge to both the food industry and regulators. Species substitution and mislabelling within fish supply chains occurs because of price incentives to misrepresent products for economic gain. Emerging authenticity technologies, such as the DNA barcoding technology that has been used to identify plants and animal (particularly fish) species through DNA sequencing, offer a potential technological solution to this information problem. However, the adoption of these authenticity technologies depends also on economic factors. The present study uses economic welfare analysis to examine the effects of species substitution and mislabelling in fish markets, and examines the feasibility of the technology for a typical retail store in Canada. It is assumed that increased accuracy of the technology in detecting fraud and enforcement of legal penalties and other associated costs would be likely to discourage cheating. Empirical results suggest that DNA barcoding technology would be feasible presently for a typical retail store only if authentication is done in a third party laboratory, as it may not be feasible on an individual retail store level once fixed and other associated costs of the technology are considered.

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