Evaluation of the Suitability of RNAscope as a Technique to Measure Gene Expression in Clinical Diagnostics: A Systematic Review

The objective of this study was to evaluate neuropeptide Y (NPY) and sea bream gonadotropin-release hormone (sbGnRH) gene expression in juvenile and adult males of Brazilian flounder. Hypothalamuses from fish were sampled for total RNA extraction. After cDNA synthesis, real-time PCR was used to measure gene expression. NPY showed approximately 2-fold increases in their mRNA levels while sbGnRH showed 3-fold increases in adult fish. These results suggest that these peptides could be involved on hypothalamic regulation of Brazilian flounder sexual maturation.

Download Full-text

High Moesin Expression Is a Predictor of Poor Prognosis of Breast Cancer: Evidence From a Systematic Review With Meta-Analysis

Frontiers in Oncology ◽

10.3389/fonc.2021.650488 ◽

2021 ◽

Vol 11 ◽

Author(s):

Xiaoli Hu ◽

Yang Liu ◽

Zhitong Bing ◽

Qian Ye ◽

Chengcheng Li

Keyword(s):

Breast Cancer ◽

Gene Expression ◽

Systematic Review ◽

Cancer Patients ◽

Poor Prognosis ◽

Data Extraction ◽

Meta Analysis ◽

Gene Expression Omnibus ◽

Breast Cancer Patients ◽

Node Metastases

Owing to metastases and drug resistance, the prognosis of breast cancer is still dismal. Therefore, it is necessary to find new prognostic markers to improve the efficacy of breast cancer treatment. Literature shows a controversy between moesin (MSN) expression and prognosis in breast cancer. Here, we aimed to conduct a systematic review and meta-analysis to evaluate the prognostic relationship between MSN and breast cancer. Literature retrieval was conducted in the following databases: PubMed, Web of Science, Embase, and Cochrane. Two reviewers independently performed the screening of studies and data extraction. The Gene Expression Omnibus (GEO) database including both breast cancer gene expression and follow-up datasets was selected to verify literature results. The R software was employed for the meta-analysis. A total of 9 articles with 3,039 patients and 16 datasets with 2,916 patients were ultimately included. Results indicated that there was a significant relationship between MSN and lymph node metastases (P < 0.05), and high MSN expression was associated with poor outcome of breast cancer patients (HR = 1.99; 95% CI 1.73–2.24). In summary, there is available evidence to support that high MSN expression has valuable importance for the poor prognosis in breast cancer patients.Systematic Review Registrationhttps://inplasy.com/inplasy-2020-8-0039/.

Download Full-text

A Systematic Review of DNA Methylation and Gene Expression Studies in Posttraumatic Stress Disorder, Posttraumatic Growth, and Resilience

Journal of Traumatic Stress ◽

10.1002/jts.22472 ◽

2020 ◽

Vol 33 (2) ◽

pp. 171-180 ◽

Cited By ~ 5

Author(s):

Divya Mehta ◽

Olivia Miller ◽

Dagmar Bruenig ◽

Georgina David ◽

Jane Shakespeare‐Finch

Keyword(s):

Gene Expression ◽

Systematic Review ◽

Dna Methylation ◽

Posttraumatic Stress Disorder ◽

Posttraumatic Growth ◽

Posttraumatic Stress ◽

Stress Disorder ◽

Expression Studies ◽

Gene Expression Studies

Download Full-text

Use of Real-Time Polymerase Chain Reaction to Measure Gene Expression in Single Cells

Methods in Molecular Medicine™ - Single Cell Diagnostics ◽

10.1007/978-1-59745-298-4_11 ◽

2007 ◽

pp. 125-133 ◽

Cited By ~ 2

Author(s):

Dagan Wells

Keyword(s):

Gene Expression ◽

Polymerase Chain Reaction ◽

Real Time ◽

Single Cells ◽

Chain Reaction ◽

Polymerase Chain ◽

Measure Gene Expression

Download Full-text

Fatty acids, epigenetic mechanisms and chronic diseases: a systematic review

Lipids in Health and Disease ◽

10.1186/s12944-019-1120-6 ◽

2019 ◽

Vol 18 (1) ◽

Cited By ~ 19

Author(s):

K. González-Becerra ◽

O. Ramos-Lopez ◽

E. Barrón-Cabrera ◽

J. I. Riezu-Boj ◽

F. I. Milagro ◽

...

Keyword(s):

Gene Expression ◽

Systematic Review ◽

Fatty Acids ◽

Chronic Diseases ◽

Gene Expression Regulation ◽

Saturated Fatty Acids ◽

Dietary Fatty Acids ◽

Epigenetic Mechanisms ◽

Metabolic Alterations

Abstract Background Chronic illnesses like obesity, type 2 diabetes (T2D) and cardiovascular diseases, are worldwide major causes of morbidity and mortality. These pathological conditions involve interactions between environmental, genetic, and epigenetic factors. Recent advances in nutriepigenomics are contributing to clarify the role of some nutritional factors, including dietary fatty acids in gene expression regulation. This systematic review assesses currently available information concerning the role of the different fatty acids on epigenetic mechanisms that affect the development of chronic diseases or induce protective effects on metabolic alterations. Methods A targeted search was conducted in the PubMed/Medline databases using the keywords “fatty acids and epigenetic”. The data were analyzed according to the PRISMA-P guidelines. Results Consumption fatty acids like n-3 PUFA: EPA and DHA, and MUFA: oleic and palmitoleic acid was associated with an improvement of metabolic alterations. On the other hand, fatty acids that have been associated with the presence or development of obesity, T2D, pro-inflammatory profile, atherosclerosis and IR were n-6 PUFA, saturated fatty acids (stearic and palmitic), and trans fatty acids (elaidic), have been also linked with epigenetic changes. Conclusions Fatty acids can regulate gene expression by modifying epigenetic mechanisms and consequently result in positive or negative impacts on metabolic outcomes.

Download Full-text