In vitro growth performance of Psidium guajava and P. guineense plantlets as affected by culture medium formulations

2020 ◽  
Vol 33 (3) ◽  
pp. 435-445 ◽  
Author(s):  
Márcia Adriana Carvalho dos Santos ◽  
Mailson Monteiro do Rêgo ◽  
Manoel Abílio de Queiróz ◽  
Duanny Thais Rodrigues Caproni ◽  
Otto Herbert Schuhmacher Dietrich ◽  
...  
Keyword(s):  
Growth Performance ◽  
Culture Medium ◽  
Psidium Guajava ◽  
In Vitro Growth

scholarly journals Carbohydrates on the growth of Cattleya schilleriana Reichb.f. seedlings (Orchidaceae)

2018 ◽  
Vol 48 (7) ◽  
Author(s):  
Renato Fernandes Galdiano Júnior ◽  
Cibele Mantovani ◽  
Eliana Gertrudes de Macedo Lemos
Keyword(s):  
Survival Data ◽  
Culture Medium ◽  
Seedling Survival ◽  
In Vitro Growth ◽  
Growth Data ◽  
Carbohydrate Source ◽  
Carbohydrate Supplementation ◽  
Percentage Survival ◽  
Half Strength

ABSTRACT: The aim of the present study was to evaluate the effects of carbohydrate supplementation on the propagation of the orchid Cattleya schilleriana. The 120-d-old seedlings were subcultured in fructose-, glucose-, or sucrose-supplemented (0, 15, 30, and 45g L-1) ½ MS culture medium (half-strength macronutrient concentrations), using a completely random design with four repetitions per treatment. After 120d of treatment, root number and length, leaf number and length, and fresh weight were evaluated, and seedling survival was evaluated after 75d of acclimatization in a greenhouse. The in vitro growth data were submitted to regression analysis, whereas the percentage survival data were analyzed using ANOVA and Tukey’s test. Both in vitro growth and ex vitro survival were lowest when the plantlets were grown in the absence of a carbohydrate source and highest (>90% survival) when supplemented with glucose. According to our findings, the addition of either glucose (30g L-1) or sucrose (30g L-1) is recommended for mass propagation of C. schilleriana.

scholarly journals In vitro growth of Cattleya nobilior Rchb. f.: culture media, sealing systems and irradiance

2021 ◽  
Vol 51 ◽  
Author(s):  
Kaliana Gottschalk de Freitas ◽  
José Carlos Sorgato ◽  
Jackeline Schultz Soares ◽  
Luan Marlon Ribeiro
Keyword(s):  
Plant Height ◽  
Natural Ventilation ◽  
Culture Medium ◽  
Culture Media ◽  
Ventilation System ◽  
Light Condition ◽  
Leaf Length ◽  
In Vitro Growth ◽  
Randomized Design

ABSTRACT Some factors may influence the in vitro culture of native orchids, especially the culture medium, sealing system and light condition. This study aimed to evaluate the addition of banana pulp and peel in the formulation of culture media, sealing system and irradiance, in the in vitro growth of Cattleya nobilior Rchb. f. A completely randomized design was used in a factorial scheme with five culture media [Murashige & Skoog supplemented or not with banana pulp or peel (100 or 200g L-1)], two light conditions [3,000 K LED lamps (43 µmol m-2 s-1 or 86 µmol m-2 s-1)] and two sealing systems (natural and conventional ventilation), with six replicates of one vial each. The plant height, diameter of the largest pseudobulb, length of the largest root and of the largest leaf, fresh weight and number of leaves, roots and shoots were evaluated. The use of both the banana pulp and peel as additives in the culture medium formulation promoted the C. nobilior growth. The natural ventilation system increased the plant height, pseudobulb diameter and leaf length, while the conventional system promoted tillering.

A new experimental culture medium for cultivation of Leishmania amazonensis: its efficacy for the continuous in vitro growth and differentiation of infective promastigote forms

2010 ◽  
Vol 106 (5) ◽  
pp. 1249-1252 ◽  
Author(s):  
Igor de Almeida Rodrigues ◽  
Bianca Alcântara da Silva ◽  
André Luis Souza dos Santos ◽  
Alane Beatriz Vermelho ◽  
Celuta Sales Alviano ◽  
...  
Keyword(s):  
Culture Medium ◽  
Leishmania Amazonensis ◽  
In Vitro Growth

scholarly journals THE OPTIMIZATION OF JELLIFYING AGENT COMPOSITION ON REGENERATION OF IN VITRO OBTAINED POTATO MICROPLANTS

2016 ◽  
Vol 24 ◽  
pp. 79-82
Author(s):  
H. M. Shevaha ◽  
M. M. Kyryk ◽  
V. M. Hunchak ◽  
T. M. Oliinyk
Keyword(s):  
Growth And Development ◽  
Culture Medium ◽  
In Vitro Growth ◽  
Maize Starch ◽  
Potato Plants

Results of studies on the optimization of Murashige and Skoog culture medium withmodified maize starch for the acceleration of potato plants micropropogation are provided. Ithas been established that the method modification increases surveillance parameters, promotesactive in vitro growth and development of potato springs.

In vitro growth of Trypanosoma musculi: Requirements of cells and serum free culture medium

1986 ◽  
Vol 16 (4) ◽  
pp. 387-390 ◽  
Author(s):  
P. Vincendeau ◽  
B. Guillemain ◽  
S. Daulouede ◽  
C. Ripert
Keyword(s):  
Culture Medium ◽  
In Vitro Growth ◽  
Serum Free

scholarly journals Orchid in vitro growth as affected by nitrogen levels in the culture medium

2012 ◽  
Vol 30 (1) ◽  
pp. 119-124 ◽  
Author(s):  
Armando R Tavares ◽  
Jorge Luiz M Young ◽  
Sandra S Ori ◽  
Shoey Kanashiro ◽  
Giuseppina PP Lima ◽  
...  
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Nitrogen Concentration ◽  
Dry Weight ◽  
In Vitro Cultivation ◽  
Ionic Balance ◽  
In Vitro Growth ◽  
Nitrogen Levels ◽  
Nitrogen Concentrations

In vitro cultivation is the main propagation method for the family Orchidaceae, whereas nitrogen is the most important nutrient in the culture media. This work was carried out to study the influence of different nitrogen concentrations on the in vitro growth of the orchid Phalaenopsis amabilis. Nitrogen concentrations varied by altering the ionic balance of the Murashige & Skoog (MS) culture medium. Plants, 360 days old, were cultivated in liquid MS, modified with 7.5, 15, 30, 45, and 60 mM N. After 180 days, we assessed plant and root length, number of leaves and roots, and fresh and dry weight of leaves, roots and plants. Treatments were assigned to completely randomized plots, with four replications. Plots consisted of five three-plant flasks. The lowest nitrogen level (7.5 mM) in the medium induced root development in length, number, and fresh and dry weight. The concentration 30 mM N stimulated both emission and dry weight accumulation of leaves. The original nitrogen concentration in the MS medium (60 mM) was excessive for the in vitro growth of P. amabilis.

scholarly journals Kasugamycin on Leifsonia xyli subsp. xyli in the in vitro culture of sugarcane

2019 ◽  
Vol 49 (8) ◽  
Author(s):  
Fabio Ribeiro Garcia ◽  
Felipe Lira de Sá Cavalcante ◽  
Robson Antonio de Souza ◽  
Laureen Michelle Houllou
Keyword(s):  
Genomic Dna ◽  
Culture Medium ◽  
Bacterial Load ◽  
Dna Amplification ◽  
In Vitro Cultivation ◽  
In Vitro Growth ◽  
Shoot Number ◽  
Morphological Variables ◽  
Higher Doses

ABSTRACT: One of the significant obstacles to the growth of sugarcane production is the infection by phytopathogens, mainly by the bacterium Leifsonia xyli subsp. xyli (Lxx) causal agent of Ratoon stunting disease. Thus, this research aimed to evaluate the effects of kasugamycin on the in vitro growth of sugarcane, as well as its effect on the bacterium Lxx. Explants of strain SP791011 from sugarcane were inoculated in MS culture medium supplemented with the antimicrobial kasugamycin at concentrations of 0.00; 0.87; 1.08; 1.74 and 3.48 mL.L-1, where they remained for 30 days. After this period, the survival rate, shoot number per explant, height of the explants, phytomass, dry phytomass and phytosanitary were evaluated based on the presence of genomic DNA of Lxx. It was verified that the culture in kasugamycin influenced the morphological variables negatively; nevertheless, the antimicrobial did not demonstrate phytotoxicity to the plants. All treatments tested in this experiment were diagnosed as positive, with DNA amplification for Lxx, despite it was observed a reduction in bacterial load, suggesting that kasugamycin at higher doses can be evaluated as an attempt to eliminate the bacterium in the in vitro cultivation of sugarcane.

scholarly journals In-vitro callus induction and multiplication of inter-nodal explants in plants Dicoma tomentosa and Alhagi maurorum

2019 ◽  
Vol 9 (4-A) ◽  
pp. 212-219 ◽  
Author(s):  
Shilpa Dhaniya ◽  
Suman Kumari Parihar
Keyword(s):  
Medicinal Plants ◽  
Culture Medium ◽  
Raw Material ◽  
Nodal Segments ◽  
Ms Medium ◽  
In Vitro Growth ◽  
Primary Metabolites ◽  
Shoot Initiation ◽  
Rooting Medium

Dicoma tomentosa and Alhagi maurorum are the two medicinal plants with fast in-vitro growth. Both the plants have high economic values. Both the plants were investigated on nodal segments and on leaves. The plants were cultured in five different conditions of medium ranging from MS1- MS5. The hormones were used in these mediums in different concentrations. BAP, NAA, Kinetin, and 2,4 D were use. The MS medium in combination with BAP (2.0 and 2.0mg/ml) with NAA 0.1 mg/ml with kinetin 0.25 mg/ml with 2-4 D were taken, where BAP 1 mg/ml with 2 mg/ml of NAA, BAP 2 mg/ml with 0.5 mg/ml of NAA showed better results with callus growth and root-shoot initiation. The best rooting medium found was MS medium supplemented with IAA and IBA 0.5mg/ each. The culture medium was used in different concentrations for estimation of primary metabolites. Maximum protein and lipid percentage were noticed in leaves of both the plants. It can be concluded that both the studied plants have high medicinal importance and can be used as raw material for industry. Keywords: - Dicoma tomentosa; Alhagi maurorum; Plant hormones; MS media.

scholarly journals In vitro growth and leaf anatomy of Cattleya walkeriana (Gardner, 1839) grown in natural ventilation system

Revista CERES ◽  
2014 ◽  
Vol 61 (6) ◽  
pp. 883-890 ◽  
Author(s):  
Adriano Bortolotti da Silva ◽  
Poliana Patrícia Lima ◽  
Livia Emanuelle Simao de Oliveira ◽  
Andre Luis Moreira
Keyword(s):  
Plant Growth ◽  
Leaf Anatomy ◽  
Natural Ventilation ◽  
Culture Medium ◽  
Ventilation System ◽  
Leaf Tissue ◽  
Conventional Ventilation ◽  
In Vitro Growth ◽  
Leaf Mesophyll

Natural ventilation system facilitates gaseous exchanges in in vitro plants promoting changes in the leaf tissue, which can be evaluated through the leaf anatomy, and it allows a cultivation closer to the photoautrophic micropropagation. The objective of this work was to evaluate the effects on in vitro growth and on the leaf anatomy of Cattleya walkeriana grown in natural and conventional ventilation system with different concentrations of sucrose (0; 15; 30 and 45 L-1) combined with different cultivation systems (conventional micropropagation and natural ventilation system). The culture medium was composed of MS salts, solidified with 7 g L-1 of agar and pH adjusted to 5.8. Forty milliliters of culture medium were distributed in 250 mL flasks, autoclaved at 120 ºC for 20 minutes. The greater plant growth, as well as the greater thickness of the mesophyll was observed with the use of 20 g L-1 sucrose in natural ventilation system. Plants grown in natural ventilation system showed a thicker leaf mesophyll, which is directly related to photoautotrophic crops. The natural ventilation system induced more elliptical stomata and probably more functional formats.

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