scholarly journals Kasugamycin on Leifsonia xyli subsp. xyli in the in vitro culture of sugarcane

2019 ◽  
Vol 49 (8) ◽  
Author(s):  
Fabio Ribeiro Garcia ◽  
Felipe Lira de Sá Cavalcante ◽  
Robson Antonio de Souza ◽  
Laureen Michelle Houllou
Keyword(s):  
Genomic Dna ◽  
Culture Medium ◽  
Bacterial Load ◽  
Dna Amplification ◽  
In Vitro Cultivation ◽  
In Vitro Growth ◽  
Shoot Number ◽  
Morphological Variables ◽  
Higher Doses

ABSTRACT: One of the significant obstacles to the growth of sugarcane production is the infection by phytopathogens, mainly by the bacterium Leifsonia xyli subsp. xyli (Lxx) causal agent of Ratoon stunting disease. Thus, this research aimed to evaluate the effects of kasugamycin on the in vitro growth of sugarcane, as well as its effect on the bacterium Lxx. Explants of strain SP791011 from sugarcane were inoculated in MS culture medium supplemented with the antimicrobial kasugamycin at concentrations of 0.00; 0.87; 1.08; 1.74 and 3.48 mL.L-1, where they remained for 30 days. After this period, the survival rate, shoot number per explant, height of the explants, phytomass, dry phytomass and phytosanitary were evaluated based on the presence of genomic DNA of Lxx. It was verified that the culture in kasugamycin influenced the morphological variables negatively; nevertheless, the antimicrobial did not demonstrate phytotoxicity to the plants. All treatments tested in this experiment were diagnosed as positive, with DNA amplification for Lxx, despite it was observed a reduction in bacterial load, suggesting that kasugamycin at higher doses can be evaluated as an attempt to eliminate the bacterium in the in vitro cultivation of sugarcane.

scholarly journals Orchid in vitro growth as affected by nitrogen levels in the culture medium

2012 ◽  
Vol 30 (1) ◽  
pp. 119-124 ◽  
Author(s):  
Armando R Tavares ◽  
Jorge Luiz M Young ◽  
Sandra S Ori ◽  
Shoey Kanashiro ◽  
Giuseppina PP Lima ◽  
...  
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Nitrogen Concentration ◽  
Dry Weight ◽  
In Vitro Cultivation ◽  
Ionic Balance ◽  
In Vitro Growth ◽  
Nitrogen Levels ◽  
Nitrogen Concentrations

In vitro cultivation is the main propagation method for the family Orchidaceae, whereas nitrogen is the most important nutrient in the culture media. This work was carried out to study the influence of different nitrogen concentrations on the in vitro growth of the orchid Phalaenopsis amabilis. Nitrogen concentrations varied by altering the ionic balance of the Murashige & Skoog (MS) culture medium. Plants, 360 days old, were cultivated in liquid MS, modified with 7.5, 15, 30, 45, and 60 mM N. After 180 days, we assessed plant and root length, number of leaves and roots, and fresh and dry weight of leaves, roots and plants. Treatments were assigned to completely randomized plots, with four replications. Plots consisted of five three-plant flasks. The lowest nitrogen level (7.5 mM) in the medium induced root development in length, number, and fresh and dry weight. The concentration 30 mM N stimulated both emission and dry weight accumulation of leaves. The original nitrogen concentration in the MS medium (60 mM) was excessive for the in vitro growth of P. amabilis.

scholarly journals In vitro behaviour of Aspasia variegata, an epiphytic orchid from the Brazilian Cerrado

2013 ◽  
Vol 43 (12) ◽  
pp. 2178-2184 ◽  
Author(s):  
Vespasiano Borges de Paiva Neto ◽  
Guilherme de Oliveira Campos ◽  
Amanda Galdi Boaretto ◽  
Monica Cristina Rezende Zuffo ◽  
Mateus de Aguiar Torrezan ◽  
...  
Keyword(s):  
Activated Charcoal ◽  
Culture Medium ◽  
Culture Media ◽  
Survival Rates ◽  
In Vitro Cultivation ◽  
In Vitro Growth ◽  
Mato Grosso ◽  
Chlorophyll Contents ◽  
Coconut Fibre

Aspasia variegata occurs naturally in the savanna of the Mato Grosso do Sul State, Brazil and it has been widely collected for its beautiful flowers. Additionally, its habitat has been greatly reduced and little or no investigation of its spread has been performed. Aiming to establish a protocol to obtain seedlings of the orchid A. variegata, different compositions of culture medium were tested to identify which one provided better in vitro growth and development and to assess the influence of these media in seedling acclimatisation. Thus, seeds obtained from mature capsules were inoculated in Knudson culture medium for 120 days until the protocorm stage. They were transferred to different culture media formulations, including MS and Knudson with half or full formulation, and 3.0 and 6.0g L-1 activated charcoal were added to them or not. After 180 days of protocorm inoculation, seedlings were evaluated for length of roots and shoots, number of roots and leaves, and chlorophyll contents. After that, seedlings were transferred to trays containing a mixture of Plantmax® and coconut fibre (1:1) for acclimatisation. Best results for the in vitro growth of A. variegata were obtained with the use of MS medium supplemented with 6.0g L-1 activated charcoal. Higher levels of chlorophyll were obtained, however, in treatments containing MS salts without activated charcoal presence, and lower levels in media containing Knudson salts with the presence of activated charcoal. The seedlings originated by higher chlorophyll levels during in vitro cultivation presented the highest survival rates and better development in the acclimatisation phase.

scholarly journals In vitro Cultivation of Hancornia speciosa Gomes : The Physical Constitution of the Culture Medium, Sucrose Concentrations and Growth Conditions

2014 ◽  
Vol 23 (2) ◽  
pp. 177-187 ◽  
Author(s):  
Juliana Silva Rodrigues Cabral ◽  
Paula Sperotto Alberto ◽  
Flávia Dionísio Pereira ◽  
Edson Luiz Souchie ◽  
Fabiano Guimarães Silva
Keyword(s):  
Seed Germination ◽  
Culture Medium ◽  
Average Length ◽  
Growth Conditions ◽  
In Vitro Assays ◽  
In Vitro Cultivation ◽  
In Vitro Growth ◽  
Hancornia Speciosa ◽  
Physical Constitution

The effect of constitution of the culture medium, different concentrations of sucrose, and different growth conditions on the germination of seeds and growth of explants of "mangaba" (Hancornia speciosa Gomes) were evaluated in two in vitro assays. In assay one, the physical constitution of a medium (solid and liquid) and different concentrations of sucrose (0, 15, 30, 45, 60, 75, and 90 g/l) were tested, and seed germination was found to be variable. In assay two evaluation of the effect of different growth conditions (with and without agitation) on the in vitro growth of explants. Present results showed that "mangaba" had the highest percentage of germination and potentiality when inoculated in a liquid medium with (15 g/l) or without sucrose. The medium without agitation resulted in a better growth of explants the average length number of buds. D. O. I. http://dx.doi.org/10.3329/ptcb.v23i2.17519 Plant Tissue Cult. & Biotech. 23(2): 177-187, 2013  (December)

scholarly journals Carbohydrates on the growth of Cattleya schilleriana Reichb.f. seedlings (Orchidaceae)

2018 ◽  
Vol 48 (7) ◽  
Author(s):  
Renato Fernandes Galdiano Júnior ◽  
Cibele Mantovani ◽  
Eliana Gertrudes de Macedo Lemos
Keyword(s):  
Survival Data ◽  
Culture Medium ◽  
Seedling Survival ◽  
In Vitro Growth ◽  
Growth Data ◽  
Carbohydrate Source ◽  
Carbohydrate Supplementation ◽  
Percentage Survival ◽  
Half Strength

ABSTRACT: The aim of the present study was to evaluate the effects of carbohydrate supplementation on the propagation of the orchid Cattleya schilleriana. The 120-d-old seedlings were subcultured in fructose-, glucose-, or sucrose-supplemented (0, 15, 30, and 45g L-1) ½ MS culture medium (half-strength macronutrient concentrations), using a completely random design with four repetitions per treatment. After 120d of treatment, root number and length, leaf number and length, and fresh weight were evaluated, and seedling survival was evaluated after 75d of acclimatization in a greenhouse. The in vitro growth data were submitted to regression analysis, whereas the percentage survival data were analyzed using ANOVA and Tukey’s test. Both in vitro growth and ex vitro survival were lowest when the plantlets were grown in the absence of a carbohydrate source and highest (>90% survival) when supplemented with glucose. According to our findings, the addition of either glucose (30g L-1) or sucrose (30g L-1) is recommended for mass propagation of C. schilleriana.

scholarly journals In vitro growth of Cattleya nobilior Rchb. f.: culture media, sealing systems and irradiance

2021 ◽  
Vol 51 ◽  
Author(s):  
Kaliana Gottschalk de Freitas ◽  
José Carlos Sorgato ◽  
Jackeline Schultz Soares ◽  
Luan Marlon Ribeiro
Keyword(s):  
Plant Height ◽  
Natural Ventilation ◽  
Culture Medium ◽  
Culture Media ◽  
Ventilation System ◽  
Light Condition ◽  
Leaf Length ◽  
In Vitro Growth ◽  
Randomized Design

ABSTRACT Some factors may influence the in vitro culture of native orchids, especially the culture medium, sealing system and light condition. This study aimed to evaluate the addition of banana pulp and peel in the formulation of culture media, sealing system and irradiance, in the in vitro growth of Cattleya nobilior Rchb. f. A completely randomized design was used in a factorial scheme with five culture media [Murashige & Skoog supplemented or not with banana pulp or peel (100 or 200g L-1)], two light conditions [3,000 K LED lamps (43 µmol m-2 s-1 or 86 µmol m-2 s-1)] and two sealing systems (natural and conventional ventilation), with six replicates of one vial each. The plant height, diameter of the largest pseudobulb, length of the largest root and of the largest leaf, fresh weight and number of leaves, roots and shoots were evaluated. The use of both the banana pulp and peel as additives in the culture medium formulation promoted the C. nobilior growth. The natural ventilation system increased the plant height, pseudobulb diameter and leaf length, while the conventional system promoted tillering.

scholarly journals Oviposition by Schistosoma mansoni during in vitro cultivation

1996 ◽  
Vol 38 (6) ◽  
pp. 423-426 ◽  
Author(s):  
Leo Roberto Barth ◽  
Ana Paula Morais Fernandes ◽  
Vanderlei Rodrigues
Keyword(s):  
Schistosoma Mansoni ◽  
Egg Production ◽  
Culture Medium ◽  
Parasite Development ◽  
In Vitro Cultivation ◽  
Progressive Reduction ◽  
Maximum Period ◽  
Medium Time ◽  
Kinetics Of

Observation of Schistosoma mansoni oviposition during in vitro culture of adult worms for a maximum period of 10 days showed three well distinct phases in the kinetics of oviposition: an initial phase with low egg production, a period of maximum oviposition and finally a progressive reduction in the number of eggs during the late phases of culture. The kinetics of oviposition and the number of eggs laid by the parasites are influenced by the number of worm pairs per amount of RPMI 1640 medium, time of parasite development in the vertebrate host and type of serum utilized in the culture medium.

Partial purification of antigens collected during in vitro cultivation of the larval stages of Taenia pisiformis

Parasitology ◽  
1976 ◽  
Vol 72 (3) ◽  
pp. 269-279 ◽  
Author(s):  
M. D. Rickard ◽  
J. C. Katiyar
Keyword(s):  
Protective Immunity ◽  
Culture Medium ◽  
Serum Proteins ◽  
Skin Tests ◽  
Rabbit Serum ◽  
Partial Purification ◽  
In Vitro Cultivation ◽  
Normal Rabbit Serum ◽  
Skin Reactions

SummaryLarvae of Taenia pisiformis were cultured in vitro in medium containing 2·5, 5 or 20% (v/v) of normal rabbit serum (NRS). Greatest development occurred in 20% NRS, and the potency of antigens collected in medium from each culture tested by intradermal (i/d) skin tests in infected rabbits paralleled the in vitro growth rate of larvae. ‘Culture’ antigens from 5% NRS stimulated good immunity in rabbits to a challenge infection with T. pisiformis eggs, although they were poorly reactive in skin tests.T. pisiformis larvae were also cultured in 10% (v/v) of nitrates of serum reduced to one-half of its volume by passage through 300 000 MW cut oif (XM300F) or 100000 MW cut off (XM100F) ultrafiltration membranes. Larvae cultured using XM300F had growth rates comparable with those cultured in 20% NRS, and the antigens released into the culture medium had equal potency in i/d tests and in stimulating protective immunity in rabbits. Larvae did not develop in XM100F orproduce skin-reactive or protective antigens.Crude ‘culture’ antigen from cultures in 20% NRS was separated into 4 fractions by nitration on Sephadex G200. All of these fractions gave i/d skin reactions in infected rabbits. Fraction 3 (F3) was the most active, but was shown by acrylamide gel electrophoresis and immunoelectrophoresis to be highly contaminated with rabbit serum proteins. F3 was separated into fractions on DEAE-Sephadex A50, and the third fraction from this was as active as the original culture medium in i/d skin tests, but had only 5% of the original protein concentration. Electrophoresis demonstrated few serum contaminants, and 2 indistinct protein bands that were not present in a similar fraction of NRS.Neither Sephadex G200 F3 nor DEAE-Sephadex F3 stimulated protective immunity in rabbits, suggesting that antigens stimulating immunity against the establishment of T. pisiformis in rabbits and those provoking cell-mediated immune reactions may be different.

A new experimental culture medium for cultivation of Leishmania amazonensis: its efficacy for the continuous in vitro growth and differentiation of infective promastigote forms

2010 ◽  
Vol 106 (5) ◽  
pp. 1249-1252 ◽  
Author(s):  
Igor de Almeida Rodrigues ◽  
Bianca Alcântara da Silva ◽  
André Luis Souza dos Santos ◽  
Alane Beatriz Vermelho ◽  
Celuta Sales Alviano ◽  
...  
Keyword(s):  
Culture Medium ◽  
Leishmania Amazonensis ◽  
In Vitro Growth

scholarly journals Polpa de banana e sacarose no desenvolvimento in vitro de orquídeas

2021 ◽  
Vol 4 (2) ◽  
pp. 70-77
Author(s):  
Eliane Lima de = Aquino ◽  
◽  
Tarcísio Rangel do Couto ◽  
João Sebastião de Paula Araújo ◽  
◽  
...  
Keyword(s):  
Experimental Design ◽  
Seedling Growth ◽  
Culture Medium ◽  
In Vitro Cultivation ◽  
Fresh Weight ◽  
Randomized Experimental Design ◽  
Number Of Leaves ◽  
Weight Number

The objetive of this study was to evaluate the effects of adding two types of banana pulp, combined with varying concentrations of sacarose on the growth of Cattleya sp. plantlets. Hybrid LCTV-01 seedlings (Cattleya labiata rubra x Cattleya labiata semi alba) made to germinate in vitro were inoculated in an MS culture medium with half the concentration of nutrients and supplemented with 60 g.L-1 'maçã' or 'terra' banana pulp in addition to different concentrations of sacarose (10, 20 and 30 g.L-1. The entirely randomized experimental design was chosen, implemented in seven treatments, ten repetitions and eight seedlings per repetition. After 160 days of in vitro cultivation, variables of fresh weight, number of leaves, number of roots and length of the longest root were evaluated. It was found that the addition of banana pulp of any of the analyzed cultivars promoted better seedling growth. Additionally, the 20 g.L-1 sacarose concentration yielded better results for the analyzed variables.

scholarly journals THE OPTIMIZATION OF JELLIFYING AGENT COMPOSITION ON REGENERATION OF IN VITRO OBTAINED POTATO MICROPLANTS

2016 ◽  
Vol 24 ◽  
pp. 79-82
Author(s):  
H. M. Shevaha ◽  
M. M. Kyryk ◽  
V. M. Hunchak ◽  
T. M. Oliinyk
Keyword(s):  
Growth And Development ◽  
Culture Medium ◽  
In Vitro Growth ◽  
Maize Starch ◽  
Potato Plants

Results of studies on the optimization of Murashige and Skoog culture medium withmodified maize starch for the acceleration of potato plants micropropogation are provided. Ithas been established that the method modification increases surveillance parameters, promotesactive in vitro growth and development of potato springs.

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