Effect of selenium biofortification on phenolic content and antioxidant properties of Jute leaf (Corchorus olitorius)

Objective: The present study has been carried out to evaluate the diuretic and antioxidant properties of pine herb in an animal model. Materials and Methods: 45 adult male rats were randomly divided into nine groups including: groups I (the negative control), groups II (positive control, furosemide 10 mg/kg), groups III to VIII (treatment groups received 100, 200, 400 mg/kg of the aqueous extracts of bark and fruit) and group IX received the combination of aqueous extract of bark (100 mg/kg) and the fruit (100 mg/kg). The urine output, glomerular filtration rate (GFR), electrolytes, urea, and creatinine levels were evaluated . Furthermore, the phenolic content and antioxidant activity of both extracts were also assessed using 2, 2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP) and Folin–Ciocalteu methods. Results: The aqueous extracts of the pine bark and fruit increased the urinary output in a dose-dependent manner. The combination of the two extracts compared to the other extracts alone significantly increased the serum potassium level. This study also showed each extract increase creatinine clearance in a dose-dependent manner (p<0.01 and p<0.05). The increase of GFR in the combination group was not significant. The current data showed a significant increase in the total phenolic content in pine bark extract in compared with the fruit extract. Conclusion: The pine bark and fruit can be useful in the prevention and treatment of kidney stones due to the high antioxidant activity.

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Avocado-Derived Biomass: Chemical Composition and Antioxidant Potential

Proceedings ◽

10.3390/foods_2020-07750 ◽

2020 ◽

Vol 70 (1) ◽

pp. 100

Author(s):

Minerva C. García-Vargas ◽

María del Mar Contreras ◽

Irene Gómez-Cruz ◽

Juan Miguel Romero-García ◽

Eulogio Castro

Keyword(s):

Chemical Composition ◽

Phenolic Content ◽

Antioxidant Properties ◽

Total Phenolic ◽

Nutritional Properties ◽

Aqueous Fraction ◽

Extraction Solvent ◽

Main Components ◽

A Minor ◽

Derivatives Of

Avocado has become fashionable due to its great organoleptic and nutritional properties. It is consumed as a fresh product and it is also processed to obtain salad oil and guacamole. In all cases, the only usable portion is the pulp. Therefore, to be a more sustainable and profitable agribusiness, it is important to recognize which compounds from the peel and the stone waste can be converted into valuable bio-products. Therefore, their chemical composition was determined according to the National Renewable Energy Laboratory, the total phenolic content by the Folin-Ciocalteu method and the antioxidant properties by the FRAP and TEAC assays. The main components of the peel and stone were acid-insoluble lignin (35.0% and 15.3%, respectively), polymeric sugars (23.6% and 43.9%, respectively), and the aqueous extractives (15.5% and 16.9%, respectively). Both biomasses contain lipids and protein, but a minor proportion (<6%). The valorization of lignin and sugars is of interest given the high content; stones are a rich source of glucose (93.2% of the polymeric fraction), which could be used to obtain biofuels or derivatives of interest. The extractive fraction of the peel contained the highest number of phenolic compounds (4.7 g/100 g biomass), mainly concentrated in the aqueous fraction (i.e., 87%) compared to the ethanol one, which was subsequently extracted. It correlated with major antioxidant activity and, therefore, the peel can be applied to obtain antioxidants and water can be used as an environmentally friendly extraction solvent.

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Evaluation of Antioxidant Properties, Total Phenolic Content, and Biological Activities of New Tomato Hybrids of Industrial Interest

Journal of Medicinal Food ◽

10.1089/jmf.2011.0118 ◽

2012 ◽

Vol 15 (5) ◽

pp. 483-489 ◽

Cited By ~ 9

Author(s):

Giuseppina Tommonaro ◽

Rocco de Prisco ◽

Gennaro Roberto Abbamondi ◽

Stefania Marzocco ◽

Carmela Saturnino ◽

...

Keyword(s):

Total Phenolic Content ◽

Phenolic Content ◽

Biological Activities ◽

Antioxidant Properties ◽

Total Phenolic

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Phytochemical and antioxidant properties of Algerian Medicago Sativa L. Water, Ethenol and Chloroform extract

South Asian Journal of Experimental Biology ◽

10.38150/sajeb.9(3).p80-86 ◽

2019 ◽

Vol 9 (3) ◽

pp. 80-86

Author(s):

Hanane Megdad ◽

Samuel Chiomokhare ◽

Ahmed Mâ€™Hamedi ◽

Paul L Chazot

Keyword(s):

Antioxidant Activity ◽

Medicago Sativa ◽

Phenolic Content ◽

Antioxidant Properties ◽

Ethanol Extract ◽

Natural Antioxidants ◽

Chloroform Extract ◽

Total Phenolic ◽

Medicago Sativa L ◽

Ethanol Extracts

Plant natural products have a long history of being a rich source of neuroactive drugs.The total phenolic and flavonoid content, and antioxidant activity of ethanol, chloroform and aqueous extracts of AlgerianMedicagoSativaL, leaves were measured to discover new potential sources of Algerian natural antioxidants and neuroactives. All extracts were non-neurotoxic up to 1 mg/ ml for 24 h exposure. The total phenolcontent of M.Sativa extracts ranged between 38 and 69 mg GAE per g extracts. All of the extracts displayed antioxidant capacityassessed by two methods, but at different levels depending on the concentration and the extraction solvent used. The overall antioxidant potency was in the order water > chloroform> ethanol extracts, and the phenolic content exhibited a positive correlation with the antioxidant activity of M. Sativa extracts.Despite a weaker antioxidant activity, the ethanol extract elicited a calcium spike and suppressed the KCl-induced depolarisation response in neurons, indicating effect on ligand-and/or voltage gated calcium channels. In contrast, the chloroform and water extractsdisplayed no apparent direct functional effects. In conclusion, the antioxidant and functional properties related to phenolic content of Algerian Medicago Sativa L. in water and ethanol extracts, respectively.

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Effects of solvent extraction system on antioxidant activity of Lamium purpureum L.

Hemijska industrija ◽

10.2298/hemind160518047g ◽

2017 ◽

Vol 71 (5) ◽

pp. 361-370

Author(s):

Slavica Grujic ◽

Ana Dzamic ◽

Violeta Mitic ◽

Vesna Stankov-Jovanovic ◽

P.D. Marin ◽

...

Keyword(s):

Antioxidant Activity ◽

Methanol Extract ◽

Phenolic Content ◽

Radical Scavenging Activity ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Reducing Power ◽

Total Phenolic ◽

Radical Scavenger ◽

Scavenging Activity

Antioxidant and free radical scavenging activity of methanol, ethanol, ethyl acetate and chloroform extracts of aerial parts of Lamium purpureum L. was determined by DPPH, ABTS, FRAP and TRP assays. Contents of flavonoids and phenols were also investigated. The total phenolic content in the extracts, determined using Folin?Ciocalteu assay, ranged between 8.57 to 128.00 mg GAE/g d.e. while concentrations of flavonoids in the extracts varied from 24.20 to 39.80 mg QuE/g d.e. The highest phenolic content was found in methanol extract (128.00 mg GAE/g d.e.). The highest content of total flavonoids was identified in the methanol extract (39.80 mg QuE/g d.e.) and the lowest was in the chloroform (24.30 mg QuE/g d.e.). DPPH scavenging of the extracts was determined and obtained IC50 values ranged from 0.12 to 3.12 mg/mL of solution. The values of ABTS radical scavenging activity ranged from 0.35 to 1.80 mg AA/g. The highest ABTS antiradical activity was registered for methanol extract. The FRAP value was found within the range 0.08 to 1.04 ?mol Fe/mg. The best radical scavenger was methanol (1.04 ?mol Fe/mg). In reducing power assay different extracts of L. purpureum showed increasing of activity with increased concentration, and all extracts possessed substantial dose dependent antioxidant activity. The best reducing capacity was obtained with methanol extract of L. purpureum (0.0132 mg AA/mL). The results in this study confirmed that L. purpureum possesses moderate antioxidant properties.

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Application of the Folin-Ciocalteu method to the evaluation of Salvia sclarea extracts

Pharmacia ◽

10.3897/pharmacia.66.e38976 ◽

2019 ◽

Vol 66 (4) ◽

pp. 209-215 ◽

Cited By ~ 2

Author(s):

Nataliia Hudz ◽

Oksana Yezerska ◽

Mariia Shanajda ◽

Vladimira Horčinová Sedláčková ◽

Piotr P. Wieczorek

Keyword(s):

Analytical Procedure ◽

Phenolic Content ◽

Antioxidant Properties ◽

Total Phenolic ◽

Optimum Conditions ◽

Pharmaceutical Development ◽

Salvia Sclarea ◽

High Antioxidant Activity ◽

High Level

Polyphenols are valuable group of phytoconstituents due to their high antioxidant activity and healing properties. Antioxidant properties of sages are attributed mainly to a high level of phenolic compounds. The aim of the present study was to elaborate an analytical procedure for the evaluation of the content of secondary metabolites of the polyphenol nature in the herb of Clary Sage (Salvia sclarea L.). Four crude extracts of Salvia sclarea herb obtained with different technologies were used to develop an analytical procedure for the total phenolic content (TPC) assay by spectrophotometric method. The optimum conditions for the analysis (time of the reaction, wavelength, and reference substances) were chosen and experimentally justified (60–80 min, 760 nm, gallic acid and rutin, respectively). Under these conditions, the developed analytical procedure is robust in the indicated time and easy for performing in phytochemical or technological laboratories. The yield of TPC from the herb of Salvia sclarea was the highest in the extracts prepared by heating at a temperature of 36–46 °C and with using the ultrasonic bath. TPC was the highest in the extract in which solvent-to-herb ratio was the least (10:1) and particle size was in the range of 2–5 mm. As a result of the studies, the analytical procedure of the determination of TPC was developed and its parameters were justified. This methodology complies with the requirements for pharmaceutical analysis to ensure the reliability of results during pharmaceutical development and routine control of Salvia sclarea extracts.

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PALYNOLOGICAL ORIGIN, PHENOLIC CONTENT AND ANTIOXIDANT PROPERTIES OF GEOPROPOLIS COLLECTED BY MANDAÇAIA (MELIPONA MANDACAIA) STINGLESS

Revista Caatinga ◽

10.1590/1983-21252020v33n126rc ◽

2020 ◽

Vol 33 (1) ◽

pp. 246-252

Author(s):

PAULO RICARDO DA SILVA ◽

TELMA MARIA GUEDES DA SILVA ◽

CELSO AMORIM CAMARA ◽

EVA MÔNICA SARMENTO DA SILVA ◽

FRANCISCO DE ASSIS RIBEIRO DOS SANTOS ◽

...

Keyword(s):

Antioxidant Activity ◽

Phenolic Compounds ◽

Phenolic Content ◽

Antioxidant Properties ◽

Northeastern Brazil ◽

Food Sources ◽

Potential Health ◽

Pollen Types ◽

Strong Relation ◽

Β Carotene

ABSTRACT The stingless bee Melipona mandacaia (Smith 1863) (mandaçaia) is found only in the region of Caatinga, Northeastern Brazil, in the states of Bahia and Pernambuco, near to São Francisco river. The aim of the present work was to determine the botanical origin and to evaluate the phenolic content and antioxidant properties (β-carotene/linoleic acid system, DPPH and ABTS scavenging) of mandaçaia geopropolis. 25 pollen types from 15 families were identified from the 9 geopropolis samples analyzed. Phenolic compounds content varied between all the geopropolis EtOH extracts, hexane, EtOAc and MeOH:H2O fractions. The main pollens found in the geopropolis samples were from the Leguminoseae family. This identification of meliponicultural plants is extremely important because it indicates the food sources used for the collection of nectar and pollen. Our results revealed that there is a strong relation between the phenolic compounds and the antioxidant activity. These results showed that total phenols of mandaçaia geopropolis may be responsible for the antioxidant activity with evidence that it's a rich source of phenols bioactive compounds with potential health benefits.

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Phenolic Content and Antioxidant Properties of Bran in 51 Wheat Cultivars

Cereal Chemistry ◽

10.1094/cchem-85-4-0544 ◽

2008 ◽

Vol 85 (4) ◽

pp. 544-549 ◽

Cited By ~ 64

Author(s):

Brij Verma ◽

Pierre. Hucl ◽

Ravindra N. Chibbar

Keyword(s):

Phenolic Content ◽

Antioxidant Properties ◽

Wheat Cultivars

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Screening and Characterization of Phenolic Compounds and Their Antioxidant Capacity in Different Fruit Peels

Foods ◽

10.3390/foods9091206 ◽

2020 ◽

Vol 9 (9) ◽

pp. 1206 ◽

Cited By ~ 2

Author(s):

Hafiz A. R. Suleria ◽

Colin J. Barrow ◽

Frank R. Dunshea

Keyword(s):

Phenolic Compounds ◽

Antioxidant Capacity ◽

Phenolic Acids ◽

Phenolic Content ◽

Antioxidant Properties ◽

Antioxidant Potential ◽

Fruit Peel ◽

Diverse Range ◽

Mango Peel

Fruit peels have a diverse range of phytochemicals including carotenoids, vitamins, dietary fibres, and phenolic compounds, some with remarkable antioxidant properties. Nevertheless, the comprehensive screening and characterization of the complex array of phenolic compounds in different fruit peels is limited. This study aimed to determine the polyphenol content and their antioxidant potential in twenty different fruit peel samples in an ethanolic extraction, including their comprehensive characterization and quantification using the LC-MS/MS and HPLC. The obtained results showed that the mango peel exhibited the highest phenolic content for TPC (27.51 ± 0.63 mg GAE/g) and TFC (1.75 ± 0.08 mg QE/g), while the TTC (9.01 ± 0.20 mg CE/g) was slightly higher in the avocado peel than mango peel (8.99 ± 0.13 mg CE/g). In terms of antioxidant potential, the grapefruit peel had the highest radical scavenging capacities for the DPPH (9.17 ± 0.19 mg AAE/g), ABTS (10.79 ± 0.56 mg AAE/g), ferric reducing capacity in FRAP (9.22 ± 0.25 mg AA/g), and total antioxidant capacity, TAC (8.77 ± 0.34 mg AAE/g) compared to other fruit peel samples. The application of LC-ESI-QTOF-MS/MS tentatively identified and characterized a total of 176 phenolics, including phenolic acids (49), flavonoids (86), lignans (11), stilbene (5) and other polyphenols (25) in all twenty peel samples. From HPLC-PDA quantification, the mango peel sample showed significantly higher phenolic content, particularly for phenolic acids (gallic acid, 14.5 ± 0.4 mg/g) and flavonoids (quercetin, 11.9 ± 0.4 mg/g), as compared to other fruit peel samples. These results highlight the importance of fruit peels as a potential source of polyphenols. This study provides supportive information for the utilization of different phenolic rich fruit peels as ingredients in food, feed, and nutraceutical products.

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Total Phenolic, Antioxidant Activities and Flavonoid Contents of Herbal Syrups

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.800.60 ◽

2019 ◽

Vol 800 ◽

pp. 60-64

Author(s):

Fredijs Dimiņš ◽

Ingrīda Augšpole

Keyword(s):

Antiradical Activity ◽

Phenolic Content ◽

Antioxidant Activities ◽

Antioxidant Properties ◽

Total Phenolic ◽

Total Flavonoids ◽

Scavenging Activity ◽

Total Phenols ◽

Total Phenolic Compounds ◽

Trolox Equivalent

The aim of the study was to characterize antioxidative properties and antiradical activity of the herbal syrups collection in Latvia. Spectrophotometry was used to characterize antioxidant properties of herbal syrups. Antiradical activity, total phenols and content of flavonoids were determined spectrophotometrically. The antiradical scavenging activity was measured by the DPPH (2.2-diphenyl-1-picrylhydrazyl) reaction. The herbal syrups antiradical scavenging activity was estimated by the amount of DPPH of reagent using in reaction with a sample of vegetable syrup (in percent) and by Trolox equivalent. The total phenolic compounds were determined by using the Folin – Ciocalteu method. The total phenolic content was expressed in mg of the gallic acid equivalents. The content of flavonoids was determined by using reaction between the AlCl3 and flavonoids. Antiradical scavenging activity by Trolox equivalent of the herbal syrups was found from 81 to be 130 mg 100 g-1 herbal syrups. The content of total phenols was found from 22 to be 282 mg GAE 100 g-1, but content of total flavonoids by quercetin equivalent was found from 1 to 44 mg QE 100 g-1. In case of increased disease problems, syrups with increased antioxidant content should be used. However, the specificity of each disease should be taken into account as well as the content of certain compounds in the plants.

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