The effect of sodium chloride and temperature on the levels of transcriptional expression of staphylococcal enterotoxin genes in Staphylococcus aureus isolates from broiler carcasses

2021 ◽  
Author(s):  
Graciela Volz Lopes ◽  
Caroline Peixoto Bastos ◽  
Wladimir Padilha da Silva
Keyword(s):  
Staphylococcus Aureus ◽  
Sodium Chloride ◽  
Staphylococcal Enterotoxin ◽  
Transcriptional Expression ◽  
Enterotoxin Genes

scholarly journals Prevalence and expression of staphylococcal enterotoxin genes in Staphylococcus aureus isolated from food poisoning outbreaks

2017 ◽  
Vol 63 (10) ◽  
pp. 834-840 ◽  
Author(s):  
Caroline Peixoto Bastos ◽  
Milena Tomasi Bassani ◽  
Marcia Magalhães Mata ◽  
Graciela Volz Lopes ◽  
Wladimir Padilha da Silva
Keyword(s):  
Staphylococcus Aureus ◽  
Food Poisoning ◽  
Rio Grande ◽  
Staphylococcal Enterotoxin ◽  
The State ◽  
Transcriptional Expression ◽  
Rio Grande Do Sul ◽  
Staphylococcal Enterotoxins ◽  
Enterotoxin Genes ◽  
Reference Strains

Staphylococcus aureus is an important pathogen of foodborne origin. The pathogen produces a variety of toxins that include the staphylococcal enterotoxins (SE). The present study aimed to evaluate the prevalence and expression of 5 SE genes (sea, seb, sec, sed, and see) in S. aureus isolated from outbreaks occurred in the state of Rio Grande do Sul, Brazil. All isolates, with the exception of 2, presented the same or higher transcriptional expression than the reference strains for at least 1 of these genes. The presence of SE genes combined with high levels of transcriptional expression suggests that 1 or more SEs were involved with the staphylococcal food poisoning outbreak analyzed in the present study.

scholarly journals Analysis of home dust for Staphylococcus aureus and staphylococcal enterotoxin genes using quantitative PCR

2017 ◽  
Vol 581-582 ◽  
pp. 750-755 ◽  
Author(s):  
Shanna Ludwig ◽  
Isabel Jimenez-Bush ◽  
Emily Brigham ◽  
Sonali Bose ◽  
Gregory Diette ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Quantitative Pcr ◽  
Staphylococcal Enterotoxin ◽  
Enterotoxin Genes

scholarly journals Prevalence and distribuion of staphylococcal enterotoxin genes among Staphylococcus aureus isolates from chicken and turkey carcasses in Algeria

2019 ◽  
Vol 69 (4) ◽  
pp. 1297 ◽  
Author(s):  
F. Mebkhout ◽  
L. Mezali ◽  
T. M. Hamdi ◽  
Z. Cantekin ◽  
Y. Ergun ◽  
...  
Keyword(s):  
Public Health ◽  
Staphylococcus Aureus ◽  
Multiplex Pcr ◽  
Staphylococcal Enterotoxin ◽  
Foodborne Illnesses ◽  
Biochemical Tests ◽  
Staphylococcal Enterotoxins ◽  
Enterotoxin Genes ◽  
Real Risk ◽  
Genes Encoding

This study is aimed to determine the prevalence of staphylococcus aureus (S.aureus) by biochemical tests in poultry carcasses. It is also intend to detect the distribution of genes for classical staphylococcal enterotoxins A, B, C, D and E (sea, seb, sec, sed and see) and for gene femA, specific for S.aureus species, using multiplex PCR. A total of 385 samples of neck skins from fresh poultry carcasses were collected during the period 2012-2013 from 16 different slaughterhouses located in the region of Algiers, Algeria. The overall prevalence of S.aureus in freshly slaughtered poultry carcasses was 41.56%, with an individual prevalence of 40.63% and 45.71% for chicken and turkey respectively. From the 95 strains of S.aureus identified by biochemical tests, 82 (86.32%) isolates were femA positive using multiplex PCR. The investigation has also revealed the presence of both enterotoxins B and D, with a predominance of seb (13.33%) followed by sed (1.67%), in the chicken carcasses while in turkey only sed was detected (4.55%) It has been found that strains of S.aureus of poultry origin can be enterotoxigenic with the predominance of genes encoding for enterotoxins seb in chicken and sed in turkey. As enterotoxins can be produced in adequate amounts to induce foodborne illnesses, these potential dangers must be considered in terms of a real risk to public health.

scholarly journals Prevalence of classical Staphylococcal enterotoxin genes of Staphylococcus aureus isolated from ready-to-eat food in Ho Chi Minh City, Vietnam

2020 ◽  
Vol 3 (4) ◽  
pp. 9-15
Author(s):  
Phuc Nguyen Do ◽  
Linh Dang Thuy ◽  
My Huong Le Thi ◽  
Thuy Tran Thi ◽  
Ngoc Bong Nguyen Thi ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Fast Food ◽  
Staphylococcal Enterotoxin ◽  
Ho Chi Minh City ◽  
Food Services ◽  
Hygiene Practices ◽  
Enterotoxin Genes ◽  
Three Samples ◽  
Random Samples ◽  
Good Hygiene

Contamination of Staphylococcus aureus in ready-to-eat (RTE) food is a leading cause of foodborne illness in Vietnam. The aim of this study was to analyze the frequency of classical Staphylococcal enterotoxin genes (sea, seb, sec, sed, and see genes) in S. aureus strains isolated from ready-to-eat food in Ho Chi Minh, Vietnam (76 strains of  S. aureus were isolated from 200 random samples of ready - to - eat (RTE) food, which include pate, pork roll, barbecue pork, roasted pork and roasted duck, 40 samples of each), all the samples were collected from different fast food services on street in district 5, 6, 7 and 8 in Ho Chi Minh City, Vietnam. All the trains of S. aureus were stored at -70oC for analyzing Staphylococcal enterotoxin genes). The obtained results in this study indicated that out of 76 S. aureus strains, there are 6 strains (7.9%) carried SE genes:  sea gene (1.32%) was detected in one pate sample (Dist. 8), sec gene was detected (3.95%) in three samples, which are two pork roll samples (Dist. 7 and 8) and one roasted duck sample (Dist. 8), and finally see gene was detected in one pate sample (Dist. 8)and in one pork roll sample (Dist.  8). This could be a serious public health risk and highlight the need to implement good hygiene practices.

scholarly journals Staphylococcus aureus and Staphylococcal Enterotoxin A in Breaded Chicken Products: Detection and Behavior during the Cooking Process

2006 ◽  
Vol 72 (11) ◽  
pp. 7057-7062 ◽  
Author(s):  
Olimpia Pepe ◽  
Giuseppe Blaiotta ◽  
Francesca Bucci ◽  
Marilena Anastasio ◽  
Maria Aponte ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Latex Agglutination ◽  
Staphylococcal Enterotoxin ◽  
Staphylococcal Enterotoxin A ◽  
Potential Health ◽  
Cooking Process ◽  
Enterotoxin Genes ◽  
Scale Preparation ◽  
Toxic Shock Syndrome Toxin ◽  
And Behavior

ABSTRACT In this study we examined the presence of Staphylococcus aureus and staphylococcal enterotoxin A (SEA) in 20 industrial breaded chicken products obtained from different retail butchers and supermarket stores in Italy. The levels of contamination in the products analyzed were quite low, although the pH values and water activities (aw) in the samples considered were in ranges favorable for S. aureus growth. As demonstrated by phenotypic and molecular characterization, in spite of the high percentage of coagulase-positive Staphylococcus strains, only three strains could be referred to the species S. aureus. Moreover, all the strains were negative in PCR assays targeting staphylococcal enterotoxin genes (seA to seE, seG to seJ, and seM to seO), as well as the toxic shock syndrome toxin 1 gene, and no SEA was detected in the retail breaded chicken samples analyzed by a reversed passive latex agglutination assay or by Western blotting. Hence, we evaluated the thermal resistance of two strains of SEA-producing S. aureus in a laboratory-scale preparation of precooked breaded chicken cutlets. The heat treatment employed in the manufacture determined the inactivation of S. aureus cells, but the preformed SEA remained active during product storage at 4°C. The presence of the staphylococci and, in particular, of S. aureus in the retail breaded chicken products analyzed is a potential health risk for consumers since the pH and aw values of these kinds of products are favorable for S. aureus growth. The thermal process used during their manufacture can limit staphylococcal contamination but cannot eliminate preformed toxins.

Distribution of Staphylococcal Enterotoxin Genes Among Staphylococcus aureus Isolates from Poultry and Humans with Invasive Staphylococcal Disease

2002 ◽  
Vol 46 (1) ◽  
pp. 132-136 ◽  
Author(s):  
Amita Hazariwala ◽  
Quesha Sanders ◽  
Charlene R. Hudson ◽  
Charles Hofacre ◽  
Stephan G. Thayer ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Staphylococcal Enterotoxin ◽  
Enterotoxin Genes

Antibiotic Resistance and Molecular Analysis of Staphylococcus aureus Isolated from Cow's Milk and Dairy Products in Northeast Brazil

2014 ◽  
Vol 77 (4) ◽  
pp. 583-591 ◽  
Author(s):  
VLADIMIR M. SILVEIRA-FILHO ◽  
ISABELLE S. LUZ ◽  
ANA PAULA F. CAMPOS ◽  
WELLINGTON M. SILVA ◽  
MARIA PALOMA S. BARROS ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Gel Electrophoresis ◽  
Dairy Products ◽  
Pulsed Field Gel Electrophoresis ◽  
Staphylococcal Enterotoxin ◽  
Cow’S Milk ◽  
Northeast Brazil ◽  
Pulsed Field ◽  
Enterotoxin Genes

This work aimed to assess the clonal distribution among 94 strains of Staphylococcus aureus isolated from cow's milk, raw cheese, and a milking machine in 12 dairy farms in northeast Brazil, by analyzing different typing methods and detecting resistance and toxigenic profiles. For the first time, isolates of this region were assessed simultaneously by the polymorphism of the 3′ -end coa gene and 16S-23S rDNA, pulsed-field gel electrophoresis, antibiotic resistance phenotyping, and toxigenic arsenal. Although pulsed-field gel electrophoresis patterns showed a wider variation (discriminatory index 0.83) than the PCR-based methods, the internal transcribed spacer–PCR proved to be a useful and inexpensive procedure for conducting epidemiological surveys of S. aureus on a regional scale. Each dairy farm had its own resistance profile, and in two herds, 63% of the strains were multiresistant, probably due to the indiscriminate use of antibiotics in bovine mastitis treatment. No methicillin-resistant S. aureus strains were detected in this study; however, 93.6% of S. aureus strains harbored variable profiles of staphylococcal enterotoxin genes seg, seh, sei, and sej. Transcriptional analysis revealed that 53.3% of staphylococcal enterotoxin genes actually transcribed, pointing out the food poisoning risk of these dairy products to consumers in the region. Based on the detection of the most prevalent clones in a herd or region, appropriate antibiotic therapy and specific immunization can be used for the treatment and control of staphylococcal mastitis.

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