Effects of inorganic iron on the thiobarbituric acid method for determination of lipid peroxides

1965 ◽  
Vol 98 (3) ◽  
pp. 643-646 ◽  
Author(s):  
R.C. McKnight ◽  
F.E. Hunter
Keyword(s):  
Lipid Peroxides ◽  
Thiobarbituric Acid ◽  
Acid Method

A modified thiobarbituric acid method for the determination of N-acetylneuraminic acid

1991 ◽  
Vol 19 (1) ◽  
pp. 56S-56S
Author(s):  
VALERIE A. KINSELLA ◽  
GERARD J. BERNS ◽  
RICHARD O'KENNEDY
Keyword(s):  
Thiobarbituric Acid ◽  
Acetylneuraminic Acid ◽  
Acid Method

Determination of Antioxidant Components and Activity of Tamarix ramosissima Comparative with Vaccinium myrtillus on Streptozotocin-diabetic Mice

2018 ◽  
Vol 69 (7) ◽  
pp. 1860-1865
Author(s):  
Luciana Teodora Rotaru ◽  
Renata Maria Varut ◽  
Mihai Banicioiu Covei ◽  
Irina Iuliana Costache ◽  
Marius Novac ◽  
...  
Keyword(s):  
Lipid Peroxides ◽  
Thiobarbituric Acid ◽  
Vaccinium Myrtillus ◽  
Tamarix Ramosissima ◽  
Diabetic Mice ◽  
Flavonoid Content ◽  
Thiobarbituric Acid Reactive Substances ◽  
Total Polyphenol

Tamarix ramosissima (Tamaricaceae) is a small tree that grows spontaneously in Europe and Asia, being considered an invasive species in geographical areas with warm climates. The chemical composition is partially elucidated, being empirically used for antiinflammatory, analgesic, antibacterial and antioxidant effect. Our study aimed to evaluate the total polyphenol and flavonoid content of vegetal extracts and to test in vivo antioxidant therapeutic effect of it, comparative with Vaccinium myrtillus, using streptozotocin-induced diabetic mice. After five weeks the animals were sacrificed and we determined erythrocyte activities of superoxide dismutase, glutathione peroxidase, glutathione reductase and level of lipid peroxides as thiobarbituric acid reactive substances. Antioxidant enzymes had highest activities in mice treated with T. ramosissima extract and the level of lipid peroxides was the lowest. The tested extract had higher content of polyphenols comparative with V. myrtillus. Our results sustain the efficiency of T. ramosissima extracts on normalizing the effects of oxidative stress in diabetes.

scholarly journals Fluorometric determination of lipid peroxides in tissues with 1,3-diphenyl-2-thiobarbituric acid.

1988 ◽  
Vol 4 (2) ◽  
pp. 207-210 ◽  
Author(s):  
Yoshihiro YOSHIMURA ◽  
Shinichi KOIKE ◽  
Hiroshi TANAKA ◽  
Kohei TAMURA ◽  
Keiko OHSAWA ◽  
...  
Keyword(s):  
Lipid Peroxides ◽  
Thiobarbituric Acid ◽  
Fluorometric Determination

Principales métodos para la determinación de la oxidación lipídica Main methods used in lipid oxidation determination

1998 ◽  
Vol 4 (6) ◽  
pp. 391-399 ◽  
Author(s):  
C. Sánchez-Moreno' ◽  
J.A. Larrauri
Keyword(s):  
Lipid Oxidation ◽  
Lipid Peroxides ◽  
Thiobarbituric Acid ◽  
Conjugated Dienes ◽  
Oxygen Absorption ◽  
Selective Determination ◽  
Chromatographic Methods ◽  
Peroxide Values ◽  
Termination Phase

Main spectrophotometric and chromatographic methods for determination of lipid oxidation in foods and biological systems are reviewed. A new classification, according to the compounds produced in the oxidation phases, is suggested as follows: measurement of oxygen absorption, conjugated dienes, peroxide values, and decomposition of lipid peroxides. Both conjugated dienes (propagation phase) and thiobarbituric acid (termination phase) methods are the most used, despite their interferences; however, chromatographic methods are more precise and selective. Determination of bioavailability and metabolic pathway of the antioxidants in the human body are problems to be solved in the devel opment of new biological antioxidants.

A Simple Method for Estimation of Lipoxygenase and Cyclo-Oxygenase Pathways in Human Platelets - The Use of Thiobarbituric Acid Reaction

1979 ◽  
Author(s):  
M. Okuma ◽  
H. Takayama ◽  
H. Uchino
Keyword(s):  
Arachidonic Acid ◽  
Thin Layer ◽  
Normal Values ◽  
Lipid Peroxides ◽  
Thiobarbituric Acid ◽  
Myeloproliferative Disorders ◽  
Human Platelets ◽  
Simple Method ◽  
Acid Reaction

As lipid peroxides are produced from arachidonic acid(AA) by platelet lipoxygenase(PLO) and cyclo-oxygenase(PCO), a simple method was developed for estimation of these enzymic pathways by quantitating the thiobarbituric acid-reacting substance(TBARS) produced by the incubation of AA with human platelets. Thin-layer radiochromatographic analysis (J. Biol.Chem. 252:5871, 1977) of lipid products obtained by incubating [1-14C] AA with washed platelets under various conditions revealed that maximal amounts of PCO products were obtained by the incubation at 37°C within 1 min around pH 7.4, while those of PLO products within 10 min around pH 6.5. The incubation at 37°C of AA with aspirin-treated platelets at pH 7.4 for 1 min produced no TBARS, while that at pH 6.5 did TBARS as a function of the incubation time (up to 10 min). Based on these findings, PLO and PCO pathways were estimated by the determination of TBARS (J.Lab.Clin.Med. 75:283, 1970) obtained by incubating AA (0.2 mM, f.c.) either with aspirin-treated or with intact washed platelets (108) at 37°C as shown in the table. Normal values (M ± SD) expresse in terms of nmols of malonaldehyde were: PLO = 1. 76 ± 0 .36 (n=l7), PCO = 1.12 ± 0.42 (n=15). Alterations in these pathways were detecetd by this method in some patients with myeloproliferative disorders.

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