Principales métodos para la determinación de la oxidación lipídica Main methods used in lipid oxidation determination

Main spectrophotometric and chromatographic methods for determination of lipid oxidation in foods and biological systems are reviewed. A new classification, according to the compounds produced in the oxidation phases, is suggested as follows: measurement of oxygen absorption, conjugated dienes, peroxide values, and decomposition of lipid peroxides. Both conjugated dienes (propagation phase) and thiobarbituric acid (termination phase) methods are the most used, despite their interferences; however, chromatographic methods are more precise and selective. Determination of bioavailability and metabolic pathway of the antioxidants in the human body are problems to be solved in the devel opment of new biological antioxidants.

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Effects of inorganic iron on the thiobarbituric acid method for determination of lipid peroxides

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism ◽

10.1016/0005-2760(65)90163-3 ◽

1965 ◽

Vol 98 (3) ◽

pp. 643-646 ◽

Cited By ~ 11

Author(s):

R.C. McKnight ◽

F.E. Hunter

Keyword(s):

Lipid Peroxides ◽

Thiobarbituric Acid ◽

Acid Method

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Determination of Antioxidant Components and Activity of Tamarix ramosissima Comparative with Vaccinium myrtillus on Streptozotocin-diabetic Mice

Revista de Chimie ◽

10.37358/rc.18.7.6432 ◽

2018 ◽

Vol 69 (7) ◽

pp. 1860-1865

Author(s):

Luciana Teodora Rotaru ◽

Renata Maria Varut ◽

Mihai Banicioiu Covei ◽

Irina Iuliana Costache ◽

Marius Novac ◽

...

Keyword(s):

Lipid Peroxides ◽

Thiobarbituric Acid ◽

Vaccinium Myrtillus ◽

Tamarix Ramosissima ◽

Diabetic Mice ◽

Flavonoid Content ◽

Thiobarbituric Acid Reactive Substances ◽

Total Polyphenol

Tamarix ramosissima (Tamaricaceae) is a small tree that grows spontaneously in Europe and Asia, being considered an invasive species in geographical areas with warm climates. The chemical composition is partially elucidated, being empirically used for antiinflammatory, analgesic, antibacterial and antioxidant effect. Our study aimed to evaluate the total polyphenol and flavonoid content of vegetal extracts and to test in vivo antioxidant therapeutic effect of it, comparative with Vaccinium myrtillus, using streptozotocin-induced diabetic mice. After five weeks the animals were sacrificed and we determined erythrocyte activities of superoxide dismutase, glutathione peroxidase, glutathione reductase and level of lipid peroxides as thiobarbituric acid reactive substances. Antioxidant enzymes had highest activities in mice treated with T. ramosissima extract and the level of lipid peroxides was the lowest. The tested extract had higher content of polyphenols comparative with V. myrtillus. Our results sustain the efficiency of T. ramosissima extracts on normalizing the effects of oxidative stress in diabetes.

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Fluorometric determination of lipid peroxides in tissues with 1,3-diphenyl-2-thiobarbituric acid.

Analytical Sciences ◽

10.2116/analsci.4.207 ◽

1988 ◽

Vol 4 (2) ◽

pp. 207-210 ◽

Cited By ~ 3

Author(s):

Yoshihiro YOSHIMURA ◽

Shinichi KOIKE ◽

Hiroshi TANAKA ◽

Kohei TAMURA ◽

Keiko OHSAWA ◽

...

Keyword(s):

Lipid Peroxides ◽

Thiobarbituric Acid ◽

Fluorometric Determination

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A Simple Method for Estimation of Lipoxygenase and Cyclo-Oxygenase Pathways in Human Platelets - The Use of Thiobarbituric Acid Reaction

10.1055/s-0039-1684771 ◽

1979 ◽

Author(s):

M. Okuma ◽

H. Takayama ◽

H. Uchino

Keyword(s):

Arachidonic Acid ◽

Thin Layer ◽

Normal Values ◽

Lipid Peroxides ◽

Thiobarbituric Acid ◽

Myeloproliferative Disorders ◽

Human Platelets ◽

Simple Method ◽

Acid Reaction

As lipid peroxides are produced from arachidonic acid(AA) by platelet lipoxygenase(PLO) and cyclo-oxygenase(PCO), a simple method was developed for estimation of these enzymic pathways by quantitating the thiobarbituric acid-reacting substance(TBARS) produced by the incubation of AA with human platelets. Thin-layer radiochromatographic analysis (J. Biol.Chem. 252:5871, 1977) of lipid products obtained by incubating [1-14C] AA with washed platelets under various conditions revealed that maximal amounts of PCO products were obtained by the incubation at 37°C within 1 min around pH 7.4, while those of PLO products within 10 min around pH 6.5. The incubation at 37°C of AA with aspirin-treated platelets at pH 7.4 for 1 min produced no TBARS, while that at pH 6.5 did TBARS as a function of the incubation time (up to 10 min). Based on these findings, PLO and PCO pathways were estimated by the determination of TBARS (J.Lab.Clin.Med. 75:283, 1970) obtained by incubating AA (0.2 mM, f.c.) either with aspirin-treated or with intact washed platelets (108) at 37°C as shown in the table. Normal values (M ± SD) expresse in terms of nmols of malonaldehyde were: PLO = 1. 76 ± 0 .36 (n=l7), PCO = 1.12 ± 0.42 (n=15). Alterations in these pathways were detecetd by this method in some patients with myeloproliferative disorders.

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Criteria for determination of lipid peroxidation in tissues: estimation in liver of mice intoxicated with carbon tetrachloride

Canadian Journal of Physiology and Pharmacology ◽

10.1139/y87-236 ◽

1987 ◽

Vol 65 (7) ◽

pp. 1503-1506 ◽

Cited By ~ 12

Author(s):

Ryungsoon Song Kim ◽

Frank S. LaBella

Keyword(s):

Lipid Peroxidation ◽

Degradation Products ◽

Lipid Peroxides ◽

Thiobarbituric Acid ◽

Time Intervals ◽

Microsomal Membranes ◽

Liver Homogenates ◽

Second Peak

The profiles of lipid peroxidation products in liver homogenates or microsomal membranes prepared from CCl4-intoxicated mice were determined by several commonly employed methods. The level of conjugated dienes peaked within 30 min and then decreased, suggesting the transitory nature of lipid peroxides in vivo. Values for thiobarbituric acid positive material peaked 30 min after CCl4 treatment, diminished thereafter for a time, and gradually rose to a new maximum at 24 h; the first peak appears to represent lipid peroxides and the second represents further degradation products including malondialdehyde. Fluorescence intensity (excitation, 360 nm; emission, 430 nm) was closely correlated with the second peak. Our findings support the involvement of lipid peroxidation in CCl4-induced hepatotoxicity in mice and emphasize the necessity for several analytical indices of lipid peroxidation performed at different time intervals.

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The effect of inorganic iron on the thiobarbituric acid method for the determination of lipid peroxides

Biochimica et Biophysica Acta (BBA) - Specialized Section on Lipids and Related Subjects ◽

10.1016/0926-6542(64)90016-2 ◽

1964 ◽

Vol 84 (4) ◽

pp. 475-477

Author(s):

E.D. Wills

Keyword(s):

Lipid Peroxides ◽

Thiobarbituric Acid ◽

Acid Method

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A New Platform for Profiling Degradation-Related Impurities Via Exploiting the Opportunities Offered by Ion-Selective Electrodes: Determination of Both Diatrizoate Sodium and Its Cytotoxic Degradation Product

Journal of AOAC International ◽

10.5740/jaoacint.16-0369 ◽

2018 ◽

Vol 101 (3) ◽

pp. 723-731 ◽

Cited By ~ 2

Author(s):

Safaa M Riad ◽

Mohamed K Abd El-Rahman ◽

Esraa M Fawaz ◽

Mostafa A Shehata

Keyword(s):

Degradation Product ◽

Degradation Products ◽

Ion Selective Electrodes ◽

Selective Determination ◽

Pharmaceutical Ingredients ◽

Related Impurities ◽

Chromatographic Methods ◽

Stability Indicating Method ◽

Impurity Profiling

Abstract Although the ultimate goal of administering active pharmaceutical ingredients (APIs) is to save countless lives, the presence of impurities and/or degradation products in APIs or formulations may cause harmful physiological effects. Today, impurity profiling (i.e., the identity as well as the quantity of impurity in a pharmaceutical) is receiving critical attention from regulatory authorities. Despite the predominant use of spectroscopic and chromatographic methods over electrochemical methods for impurity profiling of APIs, this work investigates the opportunities offered by electroanalytical methods, particularly, ion-selective electrodes (ISEs), for profiling degradation-related impurities (DRIs) compared with conventional spectroscopic and chromatographic methods. For a meaningful comparison, diatrizoate sodium (DTA) was chosen as the anionic X-ray contrast agent based on its susceptibility to deacetylation into its cytotoxic and mutagenic degradation product, 3,5-diamino-2,4,6 triiodobenzoic acid (DTB). This cationic diamino compound can be also detected as an impurity in the final product because it is used as a synthetic precursor for the synthesis of DTA. In this study, four novel sensitive and selective sensors for the determination of both DTA and its cytotoxic degradation products are presented. Sensors I and II were developed for the determination of the anionic drug, DTA, and sensors III and IV were developed for the determination of the cationic cytotoxic impurity. The use of these novel sensors not only provides a stability-indicating method for the selective determination of DTA in the presence of its degradation product, but also permits DRI profiling. Moreover, a great advantage of these proposed ISE systems is their higher sensitivity for the quantification of DTB relative to other spectroscopic and chromatographic methods, so it can measure trace amounts of DTB impurities in DTA bulk powder and pharmaceutical formulation without a need for preliminary separation.

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