Iron-binding activity of female-specific serum proteins of rainbow trout (Salmo gairdneri) and chum salmon (Oncorhyncus keta)

1976 ◽  
Vol 427 (2) ◽  
pp. 549-557 ◽  
Author(s):  
Akihiko Hara
Keyword(s):  
Rainbow Trout ◽  
Chum Salmon ◽  
Serum Proteins ◽  
Binding Activity ◽  
Salmo Gairdneri ◽  
Iron Binding ◽  
Specific Serum ◽  
Female Specific

Spawning Behavior of Chum Salmon (Oncorhynchus keta) and Rainbow Trout (Salmo gairdneri)

1975 ◽  
Vol 32 (5) ◽  
pp. 633-642 ◽  
Author(s):  
A. F. Tautz ◽  
C. Groot
Keyword(s):  
Rainbow Trout ◽  
Chum Salmon ◽  
Nest Site ◽  
Salmo Gairdneri ◽  
Oncorhynchus Keta ◽  
Spawning Behavior ◽  
Current Pattern ◽  
Chum Salmon Oncorhynchus Keta ◽  
Nest Sites ◽  
Gravel Size

Detailed accounts of the spawning behavior of chum salmon (Oncorhynchus keta) and rainbow trout (Salmo gairdneri) in laboratory flumes are provided. The behavioral activities, quivering, probing, and crossing over increase in frequency as a function of time prior to spawning whereas digging remains constant or decreases slightly. Maps of digging locations and movie films suggest nest shape and current pattern are monitored by the female, allowing her to intensify her digging activity near the center of the nest. Probing appears to be a signal to the male indicating approach of oviposition and also provides information to the female regarding the shape and suitability of the nest site. The probing act is also used in the synchronization of the male and female spawning acts. Changes in velocity do not appear to markedly affect nest construction either in terms of number of digs to oviposition or in size of nest constructed. It is inferred that velocity and gravel size are important insofar as they influence the construction of a suitable nest depression. Nest sites would appear to be selected on the basis of acceleration of flow rather than velocity per se though high limits must obviously exist.

Glomerular permselectivity to serum proteins in rainbow trout (Salmo gairdneri)

1988 ◽  
Vol 255 (3) ◽  
pp. R418-R423
Author(s):  
B. Elger ◽  
H. Ruhs ◽  
H. Hentschel
Keyword(s):  
Rainbow Trout ◽  
Glomerular Filtration ◽  
Serum Proteins ◽  
Salmo Gairdneri ◽  
Total Serum Protein ◽  
Total Serum ◽  
Protein Patterns ◽  
Gradient Gel Electrophoresis ◽  
Glomerular Hemodynamics

Changes in glomerular filtration rate (GFR) were induced in the unanesthetized rainbow trout by short-term adaptation from freshwater (FW) to brackish water (BW, 50% seawater) to investigate the effect of altered glomerular function on the glomerular permselectivity to endogenous serum proteins in vivo. Protein patterns of serum and urine were obtained by nondenaturing polyacrylamide gradient gel electrophoresis. In the serum, high-molecular-weight proteins dominate by 83.1 +/- 8.16%, and total serum protein concentrations were 3.25 +/- 0.32 (FW) and 3.12 +/- 0.16 g/100 ml (BW). The urinary protein concentration increased from 16.0 +/- 1.41 (FW) to 42.8 +/- 11.0 mg/100 ml (BW), whereas GFR and urine flow rate, both linearly related, decreased from 4.55 +/- 0.34 (FW) to 1.84 +/- 0.11 (BW) ml.h-1.kg-1 and from 2.31 +/- 0.19 (FW) to 0.56 +/- 0.02 (BW) ml.h-1.kg-1, respectively. The fractional clearance of protein increased exponentially at decreased GFR. This suggests increased glomerular filtration of serum proteins due to altered glomerular hemodynamics.

Serum Proteins and Antibody Production in Rainbow Trout (Salmo gairdneri)

1966 ◽  
Vol 23 (12) ◽  
pp. 1957-1963 ◽  
Author(s):  
George Post
Keyword(s):  
Rainbow Trout ◽  
Specific Antibody ◽  
Aeromonas Hydrophila ◽  
Serum Proteins ◽  
Immune Serum ◽  
Salmo Gairdneri ◽  
Antibody Activity ◽  
Major Protein ◽  
Humoral Antibodies ◽  
Protein Components

Rainbow trout serum was shown to be made up of three major protein components. These three major components did not coincide by electrophoretic mobility or by relative salt solubility with the classic albumin, α-, β-, and γ-globulin separation of the sera of higher animals. A component equivalent to γ-globulin of higher animals was found to be completely lacking in the sera of both immunized and non-immunized rainbow trout.Specific humoral antibodies in rainbow trout immune sera were found to be contained in the electrophoretically least mobile proteins. These proteins coincided most nearly to the β-globulins and possibly the least electrophoretically mobile α-globulins of higher animals. Salt solubility of the serum proteins containing the specific antibody assisted in this conclusion. The electrophoretically least mobile major serum protein components were found to contain all of the antibody activity. These proteins could be removed from immune serum by 20% sodium sulfate. The assumption was made that Aeromonas hydrophila antibodies in immunized rainbow trout blood serum may be made up of a complex of serum proteins with a broad range of salt solubility and electrophoretic mobilities.Serum proteins of rainbow trout could also be classified as euglobulins or pseudoglobulins. The serum proteins which contained the A. hydrophila antibodies were found to be euglobulins.

Induction of female-specific serum proteins in the sand lamprey,Lampetra reissneri, by exogenous estradiol-17β

1987 ◽  
Vol 34 (2) ◽  
Author(s):  
Shoichi Fukayama ◽  
Akihiko Hara ◽  
Takahiro Matsubara ◽  
Hiroya Takahashi
Keyword(s):  
Serum Proteins ◽  
Specific Serum ◽  
Female Specific ◽  
Estradiol 17Β

Mitochondrial DNA analysis of Pacific salmonid evolution

1986 ◽  
Vol 64 (5) ◽  
pp. 1058-1064 ◽  
Author(s):  
W. Kelley Thomas ◽  
Ruth E. Withler ◽  
Andrew T. Beckenbach
Keyword(s):  
Mitochondrial Dna ◽  
Rainbow Trout ◽  
Dna Sequences ◽  
Chinook Salmon ◽  
Related Species ◽  
Life Histories ◽  
Chum Salmon ◽  
Dna Analysis ◽  
Salmo Gairdneri ◽  
Closely Related Species

The salmonids of Pacific North America are a group of closely related species with complex life histories and interesting distribution. Previous studies of their evolution and population structure have involved classical morphological and genetic techniques. We have analysed both intra- and inter-specific variation in mitochondrial DNA sequences of the five North American species of the genus Oncorhynchus and the rainbow trout species, Salmo gairdneri. Cleavage sites for 13 different restriction enzymes were sampled, comparing an average of 48 sites per individual, or approximately 1.7% of the genome. No obvious size variation in the 16 500 ± 500 base pair length was observed. Levels of intraspecific variation detected in the chum salmon and rainbow trout were 0.24 ± 0.23 and 0.45 ± 0.26%, respectively. This variation was population specific; no variation was detected within any of the populations sampled, suggesting the existence of population substructuring. Estimates of divergence between species range from 2.46 ± 0.72% in the coho–chinook salmon comparison to 6.88 ± 1.27% between coho and chum salmon. The phylogenetic relationship among these species, based on the levels of sequence divergence, organizes the species into three distinct groups. One group includes the pink and chum salmon while a second group contains the coho and chinook salmon, as well as the rainbow trout. The sockeye salmon are distinct from both groups. Although most of these results are in accordance with classical analyses, the relationship of the rainbow trout to the coho and chinook salmon suggests different interpretations of the evolution of life histories and morphological traits in these closely related species.

Suppression of the Primary Immune Response in Rainbow Trout, Salmo gairdneri, Sublethally Exposed to Tritiated Water During Embryogenesis

1977 ◽  
Vol 34 (9) ◽  
pp. 1293-1304 ◽  
Author(s):  
J. A. Strand ◽  
M. P. Fujihara ◽  
R. D. Burdett ◽  
T. M. Poston
Keyword(s):  
Immune Response ◽  
Rainbow Trout ◽  
Total Protein ◽  
Serum Proteins ◽  
Tritiated Water ◽  
Primary Immune Response ◽  
Salmo Gairdneri ◽  
Major Protein ◽  
Antibody Synthesis ◽  
Agglutination Assay

Antibody synthesis, in response to vaccination with a 0.1-ml (1.8 × 108 cells/ml) intraperitoneally injected, heat-killed strain of Flexibacter columnaris, was employed to investigate the effect of tritium irradiation (0, 0.04, 0.4, 4.0, 40 rads total dose for 20 days during embryogenesis) on development of the primary immune response in 5-mo rainbow trout, Salmo gairdneri, reared under essentially pathogen-free conditions. Specific agglutinins to F. columnaris, determined 1-wk pre vaccination, and 3, 5, 7, 9, and 11 wk postvaccination increased rapidly in both control and irradiated fish following vaccination. Agglutinin levels in irradiated fish were suppressed to 50% of control levels at 40 rads during the 9th wk, and 50% of control levels at 4.0 rads during the 11th wk. Electrophoretic separation of serum proteins of both control and irradiated–vaccinated fish demonstrated four major protein fractions. Densitometry analyses demonstrated that fraction IV increased significantly in percent of total protein following antigenic stimulation, suggesting that fraction IV represents the specific humoral antibody to F. columnaris. The relative percent of total protein contained in fraction IV was significantly reduced in irradiated–vaccinated fish. Key words: antibody synthesis, agglutination assay, vaccination, Flexibacter columnaris, tritium, Salmo gairdneri, electrophoresis, serum protein, densitometry, primary immune response

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