High-level production of human acidic fibroblast growth factor in E. coli cells: Inhibition of DNA synthesis in rat mammary fibroblasts at high concentrations of growth factor

1990 ◽  
Vol 171 (3) ◽  
pp. 963-971 ◽  
Author(s):  
Youqiang Ke ◽  
David G. Fernig ◽  
John A. Smith ◽  
Mark C. Wilkinson ◽  
Shanez Y. Anandappa ◽  
...  
Keyword(s):  
Growth Factor ◽  
Dna Synthesis ◽  
Fibroblast Growth Factor ◽  
Fibroblast Growth ◽  
Acidic Fibroblast Growth Factor ◽  
E Coli ◽  
High Concentrations ◽  
High Level ◽  
Inhibition Of Dna Synthesis ◽  
Level Production

scholarly journals Inability of the Acidic Fibroblast Growth Factor Mutant K132E to Stimulate DNA Synthesis after Translocation into Cells

1998 ◽  
Vol 273 (18) ◽  
pp. 11164-11172 ◽  
Author(s):  
Olav Klingenberg ◽  
Antoni Wiedlocha ◽  
Andrzej Rapak ◽  
Raquel Muñoz ◽  
Pål Ø. Falnes ◽  
...  
Keyword(s):  
Growth Factor ◽  
Dna Synthesis ◽  
Fibroblast Growth Factor ◽  
Fibroblast Growth ◽  
Acidic Fibroblast Growth Factor

scholarly journals Detection of high levels of heparin binding growth factor-1 (acidic fibroblast growth factor) in inflammatory arthritic joints.

1990 ◽  
Vol 110 (4) ◽  
pp. 1417-1426 ◽  
Author(s):  
H Sano ◽  
R Forough ◽  
J A Maier ◽  
J P Case ◽  
A Jackson ◽  
...  
Keyword(s):  
T Cell ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Disease Process ◽  
Fibroblast Growth ◽  
Heparin Binding ◽  
Acidic Fibroblast Growth Factor ◽  
Streptococcal Cell Wall ◽  
Fos Protein ◽  
High Level

The synovium from patients with rheumatoid arthritis (RA) and LEW/N rats with streptococcal cell wall (SCW) arthritis, an experimental model resembling RA, is characterized by massive proliferation of synovial connective tissues and invasive destruction of periarticular bone and cartilage. Since heparin binding growth factor (HBGF)-1, the precursor of acidic fibroblast growth factor (FGF), is a potent angiogenic polypeptide and mitogen for mesenchymal cells, we sought evidence that it was involved in the synovial pathology of RA and SCW arthritis. HBGF-1 mRNA was detected in RA synovium using the polymerase chain reaction technique, and its product was immunolocalized intracellularly in both RA and osteoarthritis (OA) synovium. HBGF-1 staining was more extensive and intense in synovium of RA patients than OA and correlated with the extent and intensity of synovial mononuclear cell infiltration. HBGF-1 staining also correlated with c-Fos protein staining. In SCW arthritis, HBGF-1 immunostaining was noted in bone marrow, bone, cartilage, synovium, ligamentous and tendinous structures, as well as various dermal structures and developed early in both T-cell competent and incompetent rats. Persistent high level immunostaining of HBGF-1 was only noted in T-cell competent rats like the disease process in general. These observations implicate HBGF-1 in a multitude of biological functions in inflammatory joint diseases.

Acidic fibroblast growth factor (FGF) but not basic FGF induces sleep and fever in rabbits

1995 ◽  
Vol 269 (1) ◽  
pp. R87-R91 ◽  
Author(s):  
M. Knefati ◽  
C. Somogyi ◽  
L. Kapas ◽  
T. Bourcier ◽  
J. M. Krueger
Keyword(s):  
Growth Factor ◽  
Dna Synthesis ◽  
Fibroblast Growth Factor ◽  
Brain Temperature ◽  
Interleukin 1 ◽  
Fibroblast Growth ◽  
Sleep Regulation ◽  
Acidic Fibroblast Growth Factor ◽  
Basic Fgf ◽  
Functional Features

Acidic fibroblast growth factor (FGF) and basic FGF belong to a growth factor family. Interleukin-1, another member of that family, is involved in sleep regulation. FGFs and interleukin-1 share structural and functional features. We therefore determined whether acidic FGF and basic FGF were somnogenic. Male New Zealand White rabbits were provided with electroencephalographic (EEG) electrodes, a brain thermistor, and a lateral intracerebroventricular (icv) cannula. The animals were injected icv with isotonic NaCl (control) and on separate days with one of three doses of acidic or basic FGF (0.01, 0.1, or 1.0 micrograms) or with heat-treated acidic FGF (1.0 micrograms). The EEG, brain temperature, and motor activity were recorded for 23 h. The biological activity of basic FGF was determined in vitro by its ability to induce DNA synthesis in rat aortic smooth muscle cells. Acidic FGF induced prolonged dose-related increases in non-rapid eye movement sleep beginning in the 1st postinjection h and continuing for 12-23 h after the treatment. Acidic FGF also induced fevers of approximately 1 degree C after the 1.0 micrograms dose. Both activities of acidic FGF were lost after heat treatment. In contrast, basic FGF lacked somnogenic and pyrogenic activity, although it did induce DNA synthesis. Current results suggest that acidic FGF is part of the complex cytokine network in brain involved in sleep regulation.

Sulfation of extracellular matrices modifies growth factor effects on type II cells on laminin substrata

1998 ◽  
Vol 275 (4) ◽  
pp. L701-L708 ◽  
Author(s):  
Philip L. Sannes ◽  
Jody Khosla ◽  
Cheng-Ming Li ◽  
Ines Pagan
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Dna Synthesis ◽  
Fibroblast Growth Factor ◽  
Alveolar Epithelial Cells ◽  
Type Ii Cells ◽  
Type Ii ◽  
Fibroblast Growth ◽  
Heparin Binding ◽  
Acidic Fibroblast Growth Factor

The alveolar basement membrane contains a variety of extracellular matrix (ECM) molecules, including laminin and sulfated glycosaminoglycans of proteoglycans. These mixtures exist within microdomains of differing levels of sulfate, which may specifically interact to be key determinants of the known capacity of the type II cell to respond to certain growth factors. Isolated type II cells were exposed to either acidic fibroblast growth factor (FGF-1), basic fibroblast growth factor (FGF-2), or keratinocyte growth factor (KGF; FGF-7) on culture wells precoated with laminin alone or in combination with chondroitin sulfate (CS), high-molecular-weight heparin, or their desulfated forms. Desulfated heparin significantly elevated FGF-1- and FGF-2-stimulated DNA synthesis, whereas desulfated CS and N-desulfated heparin elevated FGF-7-stimulated DNA synthesis by type II cells on laminin substrata. When FGF-1 was mixed into the various test matrix substrata, DNA synthesis was significantly increased in all cases. These results demonstrated that decreased levels of sulfate in ECM substrata act to upregulate responses to heparin-binding growth factors by alveolar epithelial cells on laminin substrata.

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