An immunological method for the purification of radiolabelled thyroxine

1978 ◽  
Vol 86 (1) ◽  
pp. 51-59 ◽  
Author(s):  
E. Cooper ◽  
C.W. Burke
Keyword(s):  
Immunological Method

Comparison of two rapid tests for the detection of legionellaceae in water: a microbiological-immunological method and a commercial gene-probe testkit

1995 ◽  
Vol 31 (5-6) ◽  
pp. 403-406 ◽  
Author(s):  
E. Frahm ◽  
U. Obst
Keyword(s):  
Monoclonal Antibodies ◽  
Conventional Method ◽  
Standard Method ◽  
False Positive ◽  
Gene Probe ◽  
Immunological Method ◽  
Probe Test ◽  
Rapid Tests ◽  
Positive Results

Two recently developed Legionella detection tests, a microbiological-immunological method based on monoclonal antibodies (carried out as a colony-blot assay) and a commercial gene-probe testkit (the EnvironAmp Legionella Kit), are compared with the standard method. The colony-blot assay is faster than the conventional method; the gene-probe test is much faster still and is the most sensitive, but in consequence is at greater risk of false-positive results.

scholarly journals An immunological method of fractionation of ribosomes from the chick lens

1970 ◽  
Vol 117 (3) ◽  
pp. 63P-63P ◽  
Author(s):  
Ruth M. Clayton ◽  
D. E. S. Truman ◽  
J. C. Campbell
Keyword(s):  
Immunological Method ◽  
Chick Lens

Prenatal Diagnosis of Homozygous αº-Thalassemia by an Immunological Method

1994 ◽  
Vol 731 (1) ◽  
pp. 193-196 ◽  
Author(s):  
L. F. BERNINI ◽  
H. H. H. KANHAI ◽  
M. LOSEKOOT ◽  
P. GIORDANO ◽  
C. L. HARTEVELD
Keyword(s):  
Prenatal Diagnosis ◽  
Immunological Method

scholarly journals Comparative study on glucocerebrosidase in spleens from patients with Gaucher disease

1990 ◽  
Vol 269 (1) ◽  
pp. 93-100 ◽  
Author(s):  
J M F G Aerts ◽  
W E Donker-Koopman ◽  
S Brul ◽  
S Van Weely ◽  
M C Sa Miranda ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Comparative Study ◽  
Molecular Mass ◽  
Gaucher Disease ◽  
Specific Activity ◽  
Normal Subjects ◽  
Accurate Assessment ◽  
Residual Enzyme Activity ◽  
Immunological Analysis ◽  
Immunological Method

In Gaucher disease (glucosylceramide lipidosis), deficiency of glucocerebrosidase causes pathological storage of glucosylceramide, particularly in the spleen. A comparative biochemical and immunological analysis has therefore been made of glucocerebrosidase in spleens from normal subjects (n = 4) and from Gaucher disease patients with non-neuronopathic (n = 5) and neuronopathic (n = 5) phenotypes. The spleens from all Gaucher disease patients showed markedly decreased glucocerebrosidase activity. Discrimination of different phenotypes of Gaucher disease was not possible on the basis of the level of residual enzyme activity, or by measurements, using the immunopurified enzyme, of kinetic constants, pI or molecular mass forms. A severe decrease was found in the specific activity of glucocerebrosidase purified to homogeneity from the spleen of a patient with the non-neuronopathic phenotype of Gaucher disease, as compared with that of the enzyme purified from the spleen of a normal subject. This finding was confirmed by an immunological method developed for accurate assessment of the relative enzyme activity per molecule of glucocerebrosidase protein. The method revealed that the residual enzyme in the spleens of all investigated patients with a non-neuronopathic course of Gaucher disease had a more than 7-fold decreased activity of glucocerebrosidase (measured in the presence of taurocholate) per molecule of enzyme, and that the concentration of glucocerebrosidase molecules in the spleens of these patients was near normal. Observations made with immunoblotting experiments were consistent with these findings. In contrast, in the spleens of patients with neuronopathic phenotypes of Gaucher disease, the concentration of glucocerebrosidase molecules was severely decreased.

AN IMMUNOLOGICAL PREGNANCY TEST

1960 ◽  
Vol XXXV (II) ◽  
pp. 261-267 ◽  
Author(s):  
Leif Wide ◽  
Carl A. Gemzell
Keyword(s):  
Urinary Excretion ◽  
Early Pregnancy ◽  
Human Urine ◽  
Haemagglutination Inhibition ◽  
Chorionic Gonadotrophin ◽  
Menstrual Period ◽  
Pregnancy Test ◽  
Immunological Method ◽  
Inhibition Reaction

ABSTRACT An immunological method for the assay of chorionic gonadotrophin (HCG) in human urine has been described in detail. The method is useful as a simple and rapid pregnancy test and can be applied for quantitative determinations of HCG in urine. Morning urine from 306 women were examined; 212 were found to be pregnant by the haemagglutination inhibition reaction and the pregnancy was confirmed by ordinary pregnancy tests; 94 women were not pregnant and the urine of these women gave in no case a haemagglutination inhibition reaction. Quantitative determinations of HCG were performed in the urine of 103 women in early pregnancy. The urinary excretion of HCG increased following the missed menstrual period and reached in the 8th week of pregnancy a level of about 160.000 IU of HCG per liter of urine.

URINARY EXCRETION OF LUTEINIZING HORMONE IN BOYS AND ADULT MEN

1970 ◽  
Vol 46 (2) ◽  
pp. 229-236 ◽  
Author(s):  
N. SCIARRA ◽  
U. LEONE
Keyword(s):  
Luteinizing Hormone ◽  
Urinary Excretion ◽  
Haemagglutination Inhibition ◽  
Adult Child ◽  
Mean Value ◽  
Sharp Rise ◽  
Adult Men ◽  
Immunological Method ◽  
The Mean

SUMMARY The daily urinary excretion of luteinizing hormone (LH) was determined in 15 boys, aged 5–11 yr., and in 15 adult men, aged 18–65 yr., by an immunological method using the haemagglutination inhibition system. The hormone was detected in every subject investigated. The mean value for urinary LH excretion in boys was equivalent to 3·4 i.u./24 hr. (range 1·3–6·5) and was 29·3 i.u./24 hr. in adults (range 15·4–44·6). The mean adult: child ratio was 8·6. There was a significant increase in LH output with age in both the boys and the men; the rate of this increase was the same in both groups. However, there was a sharp rise in hormone output at about the onset of puberty.

Creatine kinase isoenzyme patterns in human tissue obtained at surgery.

1976 ◽  
Vol 22 (2) ◽  
pp. 173-175 ◽  
Author(s):  
S H Tsung
Keyword(s):  
Creatine Kinase ◽  
Human Tissue ◽  
Creatine Kinase Isoenzyme ◽  
Quantitative Distribution ◽  
Immunological Method ◽  
Creatine Kinase Isoenzymes ◽  
Isoenzyme Patterns ◽  
Postmortem Autolysis

Abstract Chromatography on DEAE-Sephadex A-50 was applied to study the quantitative distribution of creatine kinase isoenzymes in extracts of human tissue obtained during surgery. The results are compared with those determined by an immunological method [Clin. Chim. Acta 58, 223 (1975)]. Conflicting results for some organs as reported by the two methods are probably attributable to postmortem autolysis.

Fibrinolysis After Multiple Trauma In Man. Clinical And Methodological Aspects

1981 ◽  
Author(s):  
A-C Teger-Nilsson ◽  
E Gyzander ◽  
A Medegård ◽  
B Risberq ◽  
B Wiman
Keyword(s):  
Traffic Accidents ◽  
Soft Tissues ◽  
Bone Fractures ◽  
Degradation Products ◽  
Clinical Situation ◽  
Fibrinolytic System ◽  
Phase Reaction ◽  
Chromogenic Substrate ◽  
Immunological Method ◽  
Activity Measurements

Fifteen patients with at least two bone fractures and damages of soft tissues, mainly after traffic accidents, were investigated for changes in the fibrinolytic system. Seventeen healthy students were controls. The patients were conventionally treated in an intensive care unit. Blood samples, in all 140, were taken on the day of admission and then daily for about one week. The blood was analysed for plasminogen with electroimmunoassay and with chromogenic substrate after streptokinase activation, for α 2-antiplasmin with electroimmunoassay and two types of activity measurements (chromogenic substrate), for α 2-antiplasmin - plasmin complexes with RIA technique, and for α 2-macroglobulin with electroimmunoassay and activity measurements. In addition fibrinogen, fibrinogen-fibrin degradation products and fibrinolytic activity on fibrin plates were estimated.Plasminogen was somewhat low the first days after trauma lowest 69 % ± 22 % (M ± SD), day 3]. It then slowly increased towards the end of the investigation (highest 123% ± 21%). The last days of investigation the immunological method also gave higher values than the activity method, α 2 Antiplasmin was low directly after the trauma (lowest 67% ± 18%, day 1 ) and increased gradually (highest 115% ± 19%, day 6 ). The first days after trauma immunological method gave higher values than the activity methods, α 2 Antiplasmin - plasmin complexes varied, but showed occasionally high values in particular the first day. α 2 -Macroglobulin decreased only slightly on day 5-6, when it also showed a difference between the immunological and activity methods.It is concluded that the fibrinolytic system is activated immediately after a trauma. Later there is an increase of plasminogen, α 2 antiplasmin and fibrinogen, probably due to an acute phase reaction. Some reasons for the methodological discrepancies are discussed. Serial analysis of α 2-antiplasmin activity seems to be a relevant method for evaluation of fibrinolysis in a complex clinical situation.

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