Comparison of two rapid tests for the detection of legionellaceae in water: a microbiological-immunological method and a commercial gene-probe testkit

1995 ◽  
Vol 31 (5-6) ◽  
pp. 403-406 ◽  
Author(s):  
E. Frahm ◽  
U. Obst
Keyword(s):  
Monoclonal Antibodies ◽  
Conventional Method ◽  
Standard Method ◽  
False Positive ◽  
Gene Probe ◽  
Immunological Method ◽  
Probe Test ◽  
Rapid Tests ◽  
Positive Results

Two recently developed Legionella detection tests, a microbiological-immunological method based on monoclonal antibodies (carried out as a colony-blot assay) and a commercial gene-probe testkit (the EnvironAmp Legionella Kit), are compared with the standard method. The colony-blot assay is faster than the conventional method; the gene-probe test is much faster still and is the most sensitive, but in consequence is at greater risk of false-positive results.

scholarly journals Unreliable Measurement of Basophil Maximum Leukotriene Release with the Bühlmann CAST 2000 Enzyme-Linked Immunosorbent Assay Kit

2006 ◽  
Vol 13 (3) ◽  
pp. 420-422 ◽  
Author(s):  
S. E. Burastero ◽  
C. Paolucci ◽  
D. Breda ◽  
G. Monasterolo ◽  
R. E. Rossi ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Immunosorbent Assay ◽  
False Positive ◽  
Positive Control ◽  
Enzyme Linked Immunosorbent Assay ◽  
Positive Results

ABSTRACT The Bühlmann CAST 2000 enzyme-linked immunosorbent assay is a potentially useful assay for measuring sulfidoleukotrienes released in vitro by allergen-challenged basophils. However, we observed that the positive-control reagent yielded positive signals in cell-free systems. These false-positive results depended on using a mouse anti-FcεRI monoclonal antibody and were prevented by degranulation-inducing reagents other than mouse monoclonal antibodies.

Factors Associated with False-Positive Results from Fingerstick OraQuick ADVANCE Rapid HIV 1/2 Antibody Test

2012 ◽  
Vol 11 (6) ◽  
pp. 356-360 ◽  
Author(s):  
Samara B. Rifkin ◽  
Lauren E. Owens ◽  
Jeffrey L. Greenwald
Keyword(s):  
Risk Factors ◽  
False Positive ◽  
Medical Center ◽  
Antibody Test ◽  
Blood Type ◽  
Factors Associated ◽  
Rapid Tests ◽  
Antibody Tests ◽  
Positive Results ◽  
Hiv 1

Objective: Identify factors associated with false-positive rapid HIV antibody tests. Design: This retrospective cohort study with nested case–controls involved patients tested for HIV by Boston Medical Center (BMC) affiliates. Methods: Cases had a reactive fingerstick OraQuick ADVANCE rapid HIV 1/2 antibody test and a negative Western blot. Controls had nonreactive rapid tests. We compared the prevalence of HIV risk factors between cases and the total nonreactive population and the prevalence of other clinical factors between cases and controls. Results: Of the 15 094 tests, 14 937 (98.9%) were negative and 11 (0.07%) were false positives (specificity of 99.9%). Cases were more likely to have had an HIV-infected sex partner and to be tested at certain sites compared to true negatives. More cases than controls had O-negative blood type. Conclusion: O-negative blood type and sex with an HIV-infected person may increase false-positive HIV fingerstick results. More targeted studies should examine these risk factors.

Use of chicken antibodies in enzyme immunoassays to avoid interference by rheumatoid factors

1991 ◽  
Vol 37 (3) ◽  
pp. 411-414 ◽  
Author(s):  
Anders Larsson ◽  
Alex Karlsson-Parra ◽  
J Sjöquist
Keyword(s):  
Monoclonal Antibodies ◽  
False Positive ◽  
Mammalian Species ◽  
Sandwich Elisa ◽  
Rheumatoid Factors ◽  
Igg Antibodies ◽  
False Positive Reaction ◽  
Avian Origin ◽  
Capture Antibody ◽  
Positive Results

Abstract Rheumatoid factor (RF) is a major source of interference in many immunoassays. Most immunoassays use mammalian polyclonal or monoclonal antibodies, and RF can react with IgG from mammalian species, thus causing false-positive results. In this work we have studied RF interference in a sandwich ELISA, where RF in the sample may react with both the capture antibody and the detection antibody to give a false-positive reaction. We show that rheumatoid factors do not react with chicken IgY; if the capture antibody or detection antibody (or both) is of avian origin, the interference of RF or other anti-IgG antibodies in sandwich ELISA can be avoided.

MONOCLONAL ANTIBODIES To D-DIMER: DISCREPANT LATEX AGGLUTINATION AND EIA RESULTS IN LIVER DISEASE PATIENTS

1987 ◽  
Author(s):  
JM Carr ◽  
ML McKinney ◽  
I Neuringer ◽  
J McDonagh
Keyword(s):  
Monoclonal Antibodies ◽  
Liver Disease ◽  
False Positive ◽  
Degradation Products ◽  
Metastatic Cancer ◽  
Latex Agglutination ◽  
Fibrin Monomer ◽  
Sds Page ◽  
Positive Results ◽  
D Dimer

Measurement of fibrin (ogen) degradation products (FDP) by latex agglutination with polyclonal antifibrinogen may be falsely elevated in liver disease due to the presence of “poorly clottable” fibrinogen in serum (Blood 67:1468, 1986). TSro monoclonal antibodies recognizing different epitopes of fibrin degradation fragment D-dimer (DD) are now commercially available. Because of the reported lack of crossreactivity of the DD monoclonals with fibrinogen, we employed them as tools to distinguish true fibrinolysis from false positive results due to liver disease. 17 citrated plasmas were evaluated by 5%SDS PAGE, nitrocellulose transfer and polyclonal antifibrinogen blotting for detection of FDP (X,ED,Y,D). Samples were then evaluated for ED by latex agglutination and by EIA (enzyme immunoassay) with the following results:Patients in Groups 3a and 3b were jaundiced (bilirubins 3.2-27) and had either hepatitis or cirrhosis except for one patient in Group 3a who had metastatic cancer. Assay for soluble fibrin monomer (SFM) by hemagglutination was negative in group 3b but positive in 5 of 7 patients in group 3a. Neither eta recognized fibrinogen. Purified fibrin monomer gave positive results with both EIAs. Detection of fibrinolysis by latex agglutination using monoclonal anti-DD resolves the problem of false positive results seen with polyclonal antifibrinogen in patients with liver disease. Elevated SFM in the absence of disseminated intravascular coagulation (DIC) in patients with liver disease may induce false positive ED results when measured by EIA.

scholarly journals Archived dengue serum samples produced false-positive results in SARS-CoV-2 lateral flow-based rapid antibody tests

2021 ◽  
Vol 70 (6) ◽  
Author(s):  
Himadri Nath ◽  
Abinash Mallick ◽  
Subrata Roy ◽  
Soumi Sukla ◽  
Keya Basu ◽  
...  
Keyword(s):  
Dengue Virus ◽  
False Positive ◽  
Lateral Flow ◽  
Accurate Estimation ◽  
Serum Samples ◽  
Positive Serum ◽  
Rapid Tests ◽  
Antibody Tests ◽  
Positive Results ◽  
Rapid Antibody

Co-endemicity of SARS-CoV-2 and dengue virus (DV) infection is becoming a matter of serious concern as it has been already reported that antibodies (Ab) elicited by SARS-CoV-2 infection can produce false-positive results in dengue IgG and IgM rapid tests and vice versa. Here we communicate that five of thirteen DV antibody-positive serum samples from Kolkata, archived in 2017 (predating the COVID-19 outbreak), produced false-positive results in SARS-CoV-2 IgG/IgM lateral flow-based rapid tests. Our results emphasize the importance of implementing tests with higher specificity to conduct sero-surveillance for accurate estimation of SARS-CoV-2/DV prevalence in regions where both viruses now co-exist.

Critical Evaluation of Impedance Phlebography for the Diagnosis of Deep Vein Thrombosis

1974 ◽  
Vol 31 (02) ◽  
pp. 273-278
Author(s):  
Kenneth K Wu ◽  
John C Hoak ◽  
Robert W Barnes ◽  
Stuart L Frankel
Keyword(s):  
Deep Vein Thrombosis ◽  
False Positive ◽  
False Negative ◽  
Critical Evaluation ◽  
Original Method ◽  
Vein Thrombosis ◽  
Negative Results ◽  
False Negative Results ◽  
Deep Vein ◽  
Positive Results

SummaryIn order to evaluate its daily variability and reliability, impedance phlebography was performed daily or on alternate days on 61 patients with deep vein thrombosis, of whom 47 also had 125I-fibrinogen uptake tests and 22 had radiographic venography. The results showed that impedance phlebography was highly variable and poorly reliable. False positive results were noted in 8 limbs (18%) and false negative results in 3 limbs (7%). Despite its being simple, rapid and noninvasive, its clinical usefulness is doubtful when performed according to the original method.

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