Analysis of United Kingdom purchased spices for aflatoxins using an immunoaffinity column clean-up procedure followed by high-performance liquid chroma

1993 ◽  
Vol 648 (2) ◽  
pp. 485-490 ◽  
Author(s):  
R.Colin Garner ◽  
Matthew M. Whattam ◽  
Patrick J.L. Taylor ◽  
Martin W. Stow
Keyword(s):  
United Kingdom ◽  
High Performance ◽  
High Performance Liquid Chroma ◽  
Immunoaffinity Column

Determination of fumonisins B1 and B2 in maize-based baby food products by HPLC with fluorimetric detection after immunoaffinity column clean-up

2010 ◽  
Vol 3 (2) ◽  
pp. 135-146 ◽  
Author(s):  
A. De Girolamo ◽  
D. Pereboom-de Fauw ◽  
E. Sizoo ◽  
H. van Egmond ◽  
L. Gambacorta ◽  
...  
Keyword(s):  
High Performance ◽  
Signal To Noise Ratio ◽  
Retail Market ◽  
Immunoaffinity Column ◽  
Limit Of Quantification ◽  
Citrate Buffer ◽  
Relative Standard ◽  
Phosphate Citrate Buffer ◽  
Infants And Young Children

A method for the determination of fumonisin B1 (FB1) and B2 (FB2) in different commercial maize-based products for infants and young children was developed and tested in a limited validation study involving 3 laboratories. The method used extraction at 55 °C with an acidic mixture of methanol-acetonitrile-phosphate/citrate buffer, clean-up through immunoaffinity column and fumonisin determination by high performance liquid chromatography with automated pre-column derivatisation with o-phthaldialdehyde. Recovery experiments were performed at five spiking levels in the ranges of 80-800 µg/kg FB1 and 20-200 µg/kg FB2. Mean recoveries ranged from 83 to 97% for FB1 and from 61 to 78% for FB2. Relative standard deviations for within-laboratory repeatability (RSDr) ranged from 5 to 12% for FB1 and from 8 to 13% for FB2, whereas relative standard deviation for between-laboratory reproducibility (RSDR) ranged from 6 to 10% for FB1 and from 9 to 16% for FB2. The limit of quantification of the method (signal to noise ratio of 6) was 2.8 µg/kg for FB1 and 2.2 µg/kg for FB2. Fumonisins were found in 6 out of 19 maize-based baby foods obtained from the Italian retail market at levels up to 53 µg/kg.

scholarly journals Research of Polyocarboxy Acid Water-Reducer with Modified Ployether and its Effect on Concrete’s Workability

2011 ◽  
Vol 250-253 ◽  
pp. 2032-2035 ◽  
Author(s):  
Lu Feng Pang ◽  
Xin Xin Zhou
Keyword(s):  
High Performance ◽  
High Performance Liquid Chroma ◽  
Reducing Agents ◽  
Acid Water ◽  
Concrete Mixture ◽  
Synthetic Temperature ◽  
Naphthalene Series ◽  
High Range

In this paper, polycarboxylic type high performance water reducers have been synthesized through using TPEG modified monomer. If synthetic temperature and addition time of copolymer monomers can be controlled, the performance of polycarboxylic superplasticizer will be improved. Testing results indicates that when temperature is at 60~65°C, addition time of copolymer monomers is three hours, the performance of superplasticizer is best. Through High Performance Liquid Chroma- tography (HPLC) testing, we can find it is almost the same with the famous brand abroad of the same type. The ues of polycarboxylic type high performance water reducers cooperated with naphthalene series high range water reducing agents thereby markedly improv the workability of concrete mixture.

scholarly journals Aflatoxin M1 Determination in Traditional Küp Cheese Samples of Turkey Using Immunoaffinity Column and High-Performance Liquid Chromatography

2015 ◽  
Vol 3 (12) ◽  
pp. 916 ◽  
Author(s):  
Akın Koluaçık ◽  
Göksel Tırpancı Sivri ◽  
Binnur Kaptan
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Continuous Monitoring ◽  
Aflatoxin M1 ◽  
Immunoaffinity Column ◽  
Traditional Foods ◽  
Fluorescence Detector ◽  
The Public ◽  
Maximum Tolerance

Mycotoxin occurrence in foods, especially in uncontrolled produced traditional foods causes serious health problems. In this study, traditional Küp cheese samples were collected from different part of Anatolian region in Turkey (Ankara, Nevşehir and Yozgat) and analyzed to determine Aflatoxin M1 (AFM1) level. AFM1 analysis was carried out by, immunoaffinity column (IAC) clean-up and high performance liquid chromatography (HPLC) attached with fluorescence detector (FL) The level of AFM1 in all samples was in the range of 16 and 136 ng/kg which is lower than the maximum tolerance limit of the Turkish Codex Regulations (250 ng/kg). The levels of contamination indicated that more detailed and continuous monitoring is required to increase the public health conscious and reduce consumers’ exposure to AFM1.

Sign in / Sign up

Export Citation Format

Share Document