High-performance size-exclusion chromatographic characterization of water-soluble polymeric substances produced by Phanerochaete chrysosporium from free and wheat cell wall bound 3,4-dichloroaniline

1995 ◽  
Vol 708 (2) ◽  
pp. 273-281 ◽  
Author(s):  
Enamul Hoque
Keyword(s):  
Cell Wall ◽  
Phanerochaete Chrysosporium ◽  
High Performance ◽  
Water Soluble ◽  
Size Exclusion ◽  
Size Exclusion Chromatographic ◽  
Chromatographic Characterization

Size exclusion chromatographic characterization of sodium hyaluronate fractions prepared by high energetic sonication

1993 ◽  
Vol 37 (1-2) ◽  
pp. 20-22 ◽  
Author(s):  
E. Orviský ◽  
L. Šoltés ◽  
P. Chabreček ◽  
I. Novák ◽  
M. Stančíková
Keyword(s):  
Sodium Hyaluronate ◽  
Size Exclusion ◽  
Size Exclusion Chromatographic ◽  
Chromatographic Characterization

The degradation of Indulin ATR by Streptomyces: chemical characterization of the water soluble acid precipitable products

1989 ◽  
Vol 67 (2) ◽  
pp. 350-355 ◽  
Author(s):  
P. F. Vidal ◽  
J. Bouchard ◽  
E. Chornet ◽  
R. P. Overend ◽  
H. Giroux ◽  
...  
Keyword(s):  
High Performance ◽  
Protein Complexes ◽  
Chemical Characterization ◽  
Water Soluble ◽  
Size Exclusion ◽  
Produce Water ◽  
Lignin Fraction ◽  
Exclusion Chromatography ◽  
Soluble Acid

Two strains of Streptomyces, S. badius and S. virdosporus, when incubated with kraft lignin (Indulin ATR) produce water soluble acid precipitable compounds. We show by a combination of elemental analysis, amino acid analysis, and FTIR that these are complexes of lignin fragments strongly associated with proteins. The latter, which can be separated by solvent extraction, are added by the microorganisms themselves. The lignin fraction, which represents approximately 60% of the complex weight, consists of lower molecular weight, lower methoxyl content Indulin fragments. KeyWords: lignin, Indulin, lignin–protein complexes, Fourier transform infrared spectrophotometry, high performance size exclusion chromatography, Streptomyces.

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