The degradation of Indulin ATR by Streptomyces: chemical characterization of the water soluble acid precipitable products

Two strains of Streptomyces, S. badius and S. virdosporus, when incubated with kraft lignin (Indulin ATR) produce water soluble acid precipitable compounds. We show by a combination of elemental analysis, amino acid analysis, and FTIR that these are complexes of lignin fragments strongly associated with proteins. The latter, which can be separated by solvent extraction, are added by the microorganisms themselves. The lignin fraction, which represents approximately 60% of the complex weight, consists of lower molecular weight, lower methoxyl content Indulin fragments. KeyWords: lignin, Indulin, lignin–protein complexes, Fourier transform infrared spectrophotometry, high performance size exclusion chromatography, Streptomyces.

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CHARACTERIZATION OF INULIN FROM BLACK SALSIFY (SCORZONERA HISPANICA L.) FOR FOOD AND PHARMACEUTICAL PURPOSES

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i12.28262 ◽

2018 ◽

Vol 11 (12) ◽

pp. 221 ◽

Cited By ~ 1

Author(s):

Nadezhda Petkova

Keyword(s):

High Performance ◽

Chemical Characterization ◽

Degree Of Polymerization ◽

Size Exclusion ◽

Future Application ◽

Coating Agent ◽

Swelling Capacity ◽

Exclusion Chromatography ◽

Water Holding

Objective: The aim of the current study was to evaluate the black salsify (Scorzonera hispanica L.) as a potential source of inulin and to characterize the physicochemical properties of isolated polysaccharide.Methods: The carbohydrate content in its roots and leaves was analyzed by high-performance liquid chromatography with refractive index detection (HPLC-RID) method. Microwave-assisted extraction was performed for isolation of inulin from black salsify roots. The obtained polysaccharide was characterized by HPLC-RID method, HPLC size-exclusion chromatography, and Fourier transformed-infrared spectroscopy. Functional properties as swelling capacity, solubility, and water-holding and oil-holding capacities (OHCs) were also evaluated.Results: Black salsify (S. hispanica L.) roots were evaluated as a rich source of inulin (22% dw) and 1-kestose (6.25 g/100 g dw). The isolated inulin (yield 20%) was characterized with average degree of polymerization 17, with polydispersity index (1.04) that was near to medium-chained inulin. This polysaccharide showed better OHC than water-holding capacity, and it was characterized with swelling capacity 0.5 ml/g sample.Conclusion: For the first time, inulin was isolated from black salsify roots. The chemical characterization of inulin reveals the potential of this plant to be used as a valuable source of this polysaccharide for future application in food technology and pharmaceutical industry for dietary fibers, stabilizer, and coating agent.

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Chemical characterization of polymerized products formed in the reactions of matairesinol and pinoresinol with the stable radical 2,2-diphenyl-1-picrylhydrazyl

Holzforschung ◽

10.1515/hf.2011.151 ◽

2012 ◽

Vol 66 (3) ◽

Cited By ~ 9

Author(s):

Annika I. Smeds ◽

Patrik C. Eklund ◽

Evanthia Monogioudi ◽

Stefan M. Willför

Keyword(s):

High Performance ◽

Chemical Characterization ◽

Stable Radical ◽

Size Exclusion ◽

Evaporative Light Scattering Detection ◽

Light Scattering Detection ◽

Evaporative Light Scattering ◽

Exclusion Chromatography ◽

Tof Ms

Abstract Polymerization reactions of the lignans matairesinol and pinoresinol with the stable radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) were studied. The reactions were rapid and almost quantitative. The molecular weight distribution (MWD) of the formed lignan dimers and polymers was determined by high-performance size-exclusion chromatography (HPSEC) combined with evaporative light-scattering detection (ELSD), and the compounds were characterized by HPLC-MS, pyrolysis-GC/MS, NMR, MALDI-ToF-MS, and GC-MS of the monomeric and dimeric units formed by KMnO4 oxidation. The yield of high-MW polymers is higher for pinoresinol (69%) than for matairesinol (43%). According to the HPSEC-ELSD analyses, the MWD is also broader for the pinoresinol polymers. The latter seem to consist mainly of hepta-hexacontalignans (MW 2.5–21.4 kDa), whereas the matairesinol polymers are mainly penta-pentadecalignans (MW 1.8–5.3 kDa). The polymers seem to contain mainly unmodified lignan units linked by 5-5′ bonds.

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