Production of antibodies against murine class II antigens using unconjugated synthetic peptides of the β chain region 63–78

1988 ◽  
Vol 113 (2) ◽  
pp. 165-174 ◽  
Author(s):  
Daniel J. McCormick ◽  
Kathleen Hillman ◽  
Chella S. David
Keyword(s):  
Synthetic Peptides ◽  
Class Ii ◽  
Class Ii Antigens ◽  
Β Chain

Notes: Partial Characterization of anti-H L A Class II Antibodies Isolated by Aid of Sepharose-Peptide Immunoadsorbents

1991 ◽  
Vol 46 (3-4) ◽  
pp. 321-324
Author(s):  
Alberto Chersi ◽  
Maria Cristina Morganti-Kossmann
Keyword(s):  
Immune Response ◽  
Amino Acid ◽  
Membrane Proteins ◽  
Synthetic Peptides ◽  
Class Ii ◽  
Late Response ◽  
Early Immune Response ◽  
Hla Dq ◽  
Class Ii Antigens

Synthetic peptides selected from HLA-DQ and HLADP glycoproteins were coupled to Sepharose, and used for the isolation of anti-HLA Class II antibodies from the immune sera of rabbits immunized with human lymphoblastoid cells expressing Class II antigens. Antibodies from early and late bleedings displayed remarkable differences in affinity for peptides and for soluble membrane proteins: these differences might be due to an early immune response directed preferentially against surface linear determinants, and to a late response to assembled (discontinuous) sites. The possibility that such antibodies might be used for the identification of amino acid stretches involved in the formation of the same assembled determinant is considered.

scholarly journals Immunocytochemical identification of bovine Langerhans cells by use of a monoclonal antibody directed against class II MHC antigens.

1988 ◽  
Vol 36 (8) ◽  
pp. 991-995 ◽  
Author(s):  
L A Bryan ◽  
P J Griebel ◽  
D M Haines ◽  
W C Davis ◽  
J R Allen
Keyword(s):  
Monoclonal Antibody ◽  
Langerhans Cells ◽  
Staining Method ◽  
Class Ii ◽  
Immunocytochemical Staining ◽  
Microscopic Technique ◽  
Mhc Antigens ◽  
Class Ii Mhc ◽  
Class Ii Mhc Antigens ◽  
Class Ii Antigens

We undertook a study to develop a reliable light microscopic technique for identifying Langerhans cells (LC) in bovine epidermis. Monoclonal antibodies (MCA) detecting bovine class II MHC antigens were used in conjunction with an avidin-biotin-peroxidase complex (ABC) immunocytochemical staining method. The specificity of the MCA for LC was confirmed ultrastructurally by use of gold-labeled second antibody. Epidermal sheets and epidermal single-cell suspensions examined by light microscopy confirm that bovine epidermal LC express class II antigens. Anti-bovine class II MCA is a dependable reagent for identification of LC in normal bovine epidermis.

A rapid and reliable in vivo method for anti-GPLA (class I and class II antigens) antibody titration and GPLA typing

1983 ◽  
Vol 59 (2) ◽  
pp. 205-215 ◽  
Author(s):  
Thérèse Neveu ◽  
Michèle Lefroit-Joliy ◽  
Guy André Voisin
Keyword(s):  
Class Ii ◽  
Class I ◽  
Class Ii Antigens

scholarly journals Inhibitor (IK) of IFN-γ induced HLA class II antigens expression also inhibits HLA class II constitutive expression in the human Raji B cell line

Oncogene ◽  
1997 ◽  
Vol 14 (12) ◽  
pp. 1453-1461 ◽  
Author(s):  
Jocelyn Vedrenne ◽  
Eric Assier ◽  
Raffaele Pereno ◽  
Haniaa Bouzinba-Segard ◽  
Bruno Azzarone ◽  
...  
Keyword(s):  
B Cell ◽  
Cell Line ◽  
Constitutive Expression ◽  
Class Ii ◽  
Hla Class Ii ◽  
Ifn Γ ◽  
Class Ii Antigens

THE DETAILED DISTRIBUTION OF MHC CLASS II ANTIGENS IN NORMAL HUMAN ORGANS

1984 ◽  
Vol 38 (3) ◽  
pp. 293-298 ◽  
Author(s):  
A. S. DAAR ◽  
S. V. FUGGLE ◽  
J. W. FABRE ◽  
A. TING ◽  
P. J. MORRIS
Keyword(s):  
Mhc Class Ii ◽  
Class Ii ◽  
Human Organs ◽  
Normal Human ◽  
Mhc Class Ii Antigens ◽  
Class Ii Antigens
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