Uptake of heavy metals by Plectonema boryanum (cyanophyceae) into cellular components, especially polyphosphate bodies: An X-ray energy dispersive study

1982 ◽  
Vol 27 (2) ◽  
pp. 119-127 ◽  
Author(s):  
Thomas E. Jensen ◽  
Mike Baxter ◽  
Joseph W. Rachlin ◽  
Vandana Jani
1998 ◽  
Vol 4 (S2) ◽  
pp. 262-263
Author(s):  
J.J. Goldberg ◽  
T.E. Jensen

The elemental composition of polyphosphate bodies (PPB’s) and other components of the cell have previously been determined by using energy dispersive X-ray spectrometers(EDX). In this present study we perform a quantitative analysis or normally grown cells and cells that were grown in the overplus phenomenon.Cells of Plectonema boryanum were grown in modified Fitzgerald's media and harvested after a logarithmic growth phase of 14 days. The overplus cells were grown as has been previously described2. The cells were exposed to l00ppm of lead, as well as other metals separately, for one hour. The cells were then fixed and embedded in EPON according to Luft's procedure.For X-ray analysis cells were first located using the TEM mode, and then the microscope was switched to the STEM mode. Analysis of cell components were carried out using the spot mode (75Kv).


1979 ◽  
Vol 69 (2) ◽  
pp. 232-238 ◽  
Author(s):  
Barbara B. Doonan ◽  
Richard E. Crang ◽  
Thomas E. Jensen ◽  
Michael Baxter

2002 ◽  
Vol 30 (4) ◽  
pp. 669-672 ◽  
Author(s):  
M. Merroun ◽  
C. Hennig ◽  
A. Rossberg ◽  
G. Geipel ◽  
T. Reich ◽  
...  

A combination of EXAFS, transmission electron microscopy and energy-dispersive X-ray was used to conduct a molecular and atomic analysis of the uranium complexes formed by Acidithiobacillus ferrooxidans. The results demonstrate that this bacterium accumulates uranium as phosphate compounds. We suggest that at toxic levels when the uranium enters the bacterial cells, A. ferrooxidans can detoxify and efflux this metal by a process in which its polyphosphate bodies are involved.


2015 ◽  
Vol 7 (16) ◽  
pp. 6545-6551 ◽  
Author(s):  
Shigeru Taguchi ◽  
Miyabi Asaoka ◽  
Eiko Hirokami ◽  
Noriko Hata ◽  
Hideki Kuramitz ◽  
...  

A simple and rapid pre-concentration technique for the simultaneous determination of eight trace heavy metals in water by energy-dispersive X-ray fluorescence spectrometry.


1997 ◽  
Vol 16 (4) ◽  
pp. 807-812 ◽  
Author(s):  
Maria L. Carvalho ◽  
Joao, G. Ferreira ◽  
Pedro Amorim ◽  
Maria I. M. Marques ◽  
Maria T. Ramos
Keyword(s):  

2017 ◽  
Vol 7 (1) ◽  
pp. 36-39
Author(s):  
Nilay Akcay ◽  
Nevzat Batan ◽  
Yunus Cinar

Abstract In recent years, environmental safety associated with heavy metal levels of a tourism area and its surrounding areas has been one of the basic security for tourists health. Various methods have been used for measuring concentrations of heavy metal levels in these areas. Biomonitoring organisms such as mosses provide quantitative information on the quality of the environment around them, and they respond to pollution by altering their physiology or their ability to accumulate heavy metals. The aim of this study is to assess  the concentration of aluminum, manganese, iron, nickel, copper, and zinc levels in five different moss species collected around Camlihemsin district of Rize, by using Energy Dispersive X-ray Fluorescence (EDXRF) Spectrometry. The average concentrations of heavy metals in moss samples were found ranged from 3.8-8.3% for aluminum, 0.1-0.47% for manganese, 2.3-3.8% for iron, 38.8-74.2 ppm for nickel, 92.1-495.3 ppm for copper, 187.3-334 ppm for zinc. This study has shown that Abietinella abietina, Hypnum cupressiforme, Plagiomnium undulate, Rhytidium rugosum, and Thuidium tamariscinum samples were used to assess the potential contamination of Al, Mn, Fe, Ni, Cu, and Zn contamination in the region and made important contributions toward the understanding of Al, Mn, Fe, Ni, Cu, and Zn baseline data can be used for identification of changes in the levels of these heavy metals in the studied area. Keywords: Energy Dispersive X-ray Fluorescence (EDXRF) Spectrometry, heavy metals, moss, biomonitor, environment.  


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