A highly sensitive technique for staining DNA and RNA in polycrylamide gels using silver

1981 ◽  
Vol 5 (4) ◽  
pp. 219-228 ◽  
Author(s):  
Teni Boulikas ◽  
Ronald Hancock
Keyword(s):  
Sensitive Technique ◽  
Dna And Rna ◽  
Highly Sensitive

The use of droplet digital PCR in liquid biopsies: A highly sensitive technique for MYD88 p.(L265P) detection in cerebrospinal fluid

2017 ◽  
Vol 36 (2) ◽  
pp. 429-435 ◽  
Author(s):  
Laura S. Hiemcke-Jiwa ◽  
Monique C. Minnema ◽  
Joyce H. Radersma-van Loon ◽  
N. Mehdi Jiwa ◽  
Mirthe de Boer ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Digital Pcr ◽  
Droplet Digital Pcr ◽  
Liquid Biopsies ◽  
Sensitive Technique ◽  
Highly Sensitive

Revealing the unseen: the organizer region of the nucleolus

2001 ◽  
Vol 114 (17) ◽  
pp. 3199-3205 ◽  
Author(s):  
Marco Biggiogera ◽  
Manuela Malatesta ◽  
Sousan Abolhassani-Dadras ◽  
François Amalric ◽  
Lawrence I. Rothblum ◽  
...  
Keyword(s):  
Inner Core ◽  
Organizer Region ◽  
Exact Distribution ◽  
Dense Fibrillar Component ◽  
Fibrillar Center ◽  
Energy Filtering ◽  
Dna And Rna ◽  
Highly Sensitive ◽  
Fibrillar Component ◽  
First Time

We carried out a high-resolution ultrastructural analysis of the nucleolus in mouse P815 cells by combining specific DNA and RNA staining, anti-fibrillarin immunolabeling, contrast enhancement by energy filtering TEM and phosphorus mapping by ESI to visualize nucleic acids. We demonstrated that specifically contrasted DNA, fibrillarin and phosphorus overlap within the nucleolar dense fibrillar component. Moreover, we describe a ‘DNA cloud’ consisting of an inner core of DNA fibers (fibrillar center) and a periphery made of extremely thin fibrils overlapping the anti-fibrillarin immunolabeling (dense fibrillar component). This highly sensitive approach has allowed us to demonstrate, for the first time, the exact distribution of DNA within the decondensed interphase counterpart of the NOR, which includes both the fibrillar center and the dense fibrillar component.

FTIR-spectroscopy as a highly sensitive technique to study adsorption and desorption on ionic film and single crystal surfaces

1988 ◽  
Vol 95 (1-6) ◽  
pp. 105-108 ◽  
Author(s):  
Joachim Heidberg ◽  
Elisabeth Kampshoff ◽  
Helmut Stein ◽  
Helmut Weiss ◽  
Michael Warskulat
Keyword(s):  
Single Crystal ◽  
Ftir Spectroscopy ◽  
Crystal Surfaces ◽  
Adsorption And Desorption ◽  
Single Crystal Surfaces ◽  
Sensitive Technique ◽  
Highly Sensitive

Detection of cellulase and xylanase activity in isoelectric-focused gels using agar substrate gels supported on plastic film

1984 ◽  
Vol 30 (12) ◽  
pp. 1522-1525 ◽  
Author(s):  
C. R. MacKenzie ◽  
R. E. Williams
Keyword(s):  
Congo Red ◽  
Carboxymethyl Cellulose ◽  
Xylanase Activity ◽  
Plastic Film ◽  
Sensitive Technique ◽  
Highly Sensitive ◽  
Agar Substrate

A simple, rapid, and highly sensitive technique is described for the detection of endoglucanase and endoxylanase in isoelectric-focused gels. Agar substrate gels containing 0.1% carboxymethyl cellulose or xylan are prepared on GelBond® plastic film and placed in contact with an electrofocused gel containing the enzymes under study. Hydrolysis zones in substrate gels are visualized by staining with Congo red. As little as 1 ng of enzyme is detected in 5 min using this technique.

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