The antimutagenic effects of human saliva investigated with the umu-test. I. Effects of filtration and storage at low temperatures

Author(s):  
M. Okada ◽  
S. Nakamura ◽  
K. Miura ◽  
K. Morimoto
1991 ◽  
pp. 461-471 ◽  
Author(s):  
G.R. Chaplin ◽  
S.P. Cole ◽  
M. Landrigan ◽  
P.A. Nuevo ◽  
P.F. Lam ◽  
...  

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 521a-521
Author(s):  
L.E. Towill ◽  
S.A. Blackman

cryopreservation of dormant, vegetative apple buds at the National Seed Storage Laboratory is used to maintain a base collection for germplasm held in the National Clonal Germplasm Repository for apple and grape, Geneva, NY, and is performed by a method previously reported1. Growth of buds after grafting is now used to test for survival after exposure to and storage at very low temperatures (ca.-160°C). We are interested in determining if measures of respiration can be used to assess 1. the status of buds and bark used for preservation, 2. survival after different treatments related to cryopreservation, and 3. the extent of sublethal injury after treatments. A Licor 6252 CO2 analyzer was used to measure respiration. Reproducible measurements of respiration required at least 2-3 buds. Buds from winter harvested twigs (ca 45-48% moisture content) that were briefly warmed to room temperature respired at a rate of 34 umoles CO2 g-1 (dw) hr-1. Survival of buds is enhanced if twigs are dried prior to cooling. We found such treatments reduced respiration over non-dried controls. Respiration increased as the bud was rehydrated. Buds from dried twigs slowly cooled to low temperatures had levels of respiration after warming and rehydrating similar to undried, unfrozen controls. Buds from undried twigs directly placed at -196°C and warmed gave little CO2 production.


2016 ◽  
Vol 69 (2) ◽  
pp. 7955-7963 ◽  
Author(s):  
Jairo Mercado Camargo ◽  
Arnulfo Taron Dunoyer ◽  
Luis A. García-Zapateiro

The tropical fruits are sensitive to low storage temperatures, so optimal parameters have been searched for storage and transport for the purpose of maintaining its overall quality as long as possible to the consumer. The effect of different storage temperatures (6, 10, 15, 21 and 27 °C) and storage durations (0 to 20 d) on total phenolics and enzymatic activity of peroxidase (POD), catalase (CAT), and polyphenol oxidase (PPO) on sapodilla (Achras sapota L.) fruit was investigated. The extraction and quantitation of protein and phenols from fruit was performed, then the enzymatic activity of PPO, POD and CAT was determined. The concentration of total phenolics decreased in the control fruit. POD activity was 3268.7 ± 1.4 U g-1 in ripening and senescence of sapodilla stored at 27 °C. CAT activity reached a peak of 34.0 ± 0.25 U g-1 in senescence in control fruit. PPO activity remained unchanged in the ripening stage and until consumption. The best storage temperatures to prolong the post-harvest life of the sapodilla fruit were 6 °C and 10 °C when storage was at low temperatures. POD activity was inactivated during sapodilla storage at low temperatures (6 and 10 °C) and after being transferred to 27 °C the activity was reactivated. Likewise of fruits stored at 21 °C after being transferred to 27 °C the POD activity was reactive with a maximum value of 46.3 ± 0.012 U g-1. Enzyme activity decreased at low temperatures, which contributed to the preservation of the fruit, showing that the cold retards the maturation processes.


2006 ◽  
pp. 373-376
Author(s):  
M. Chatzi ◽  
V. Fragopoulou ◽  
V. Giannou ◽  
C. Tzia ◽  
I. Karayiannis

2013 ◽  
Vol 23 (4) ◽  
pp. 257-269 ◽  
Author(s):  
K.M.G. Gehan Jayasuriya ◽  
Asanga S.T.B. Wijetunga ◽  
Jerry M. Baskin ◽  
Carol C. Baskin

AbstractSeeds of legumes are generally considered to have physical dormancy and to be orthodox, but most seed biologists are unaware of the various kinds and combinations of dormancy and storage behaviour in seeds of this family. The aim of our study was to document the dormancy and storage behaviour of seeds of 100 native and introduced tropical Fabaceae species in Sri Lanka and classify them into germination/storage behaviour categories. Moisture content (MC) was < 16% for fresh seeds of 94 species and >29% for those of six. Seeds of these six species had low tolerance for desiccation and for low temperatures. Thus, seeds of six species are non-orthodox and 94 species orthodox. Nine of the 100 species were non-dormant, and 2, 3 and 86 had physiological, physiological epicotyl and physical dormancy, respectively. Six germination/storage behaviour categories were identified among the 100 species. However, as in extratropical regions of the world, orthodox storage behaviour and physical dormancy are characteristic of seeds of the majority of species of Fabaceae in tropical Sri Lanka.


Author(s):  
Vadym Paziuk

Energy efficiency of the drying process today is the main parameter that affects the choice of drying mode. Seed requires more careful selection of technology and equipment, which is associated with quality characteristics. Seed material to maintain high germination is dried at low temperatures, the operation of the grain dryer at high temperatures significantly reduces the quality and requires the development of special technology for the production and storage of seeds of cereals. The article reviews the increase in energy efficiency of equipment for drying grain seeds. Traditional seed drying technologies are too expensive and significantly increase the cost. The developed energy-efficient technologies are more aimed at drying food grain with a process at high temperature, which allows to use the high temperature potential of spent coolant at the outlet of the dryer, which can be recycled and re-directed to heat the drying agent at the dryer. When drying the seeds of cereals, it is necessary to carry out the process at low temperatures, which reduces the efficiency of the temperature potential of the spent coolant. Therefore, to increase the energy efficiency of the drying process and technology, it is proposed to use heat pumps that ensure the quality of seed material. The article also analyzes heat losses and recommends measures to reduce losses during drying, which provide for the use of heat pump units. Energy-efficient technology of drying and storage of seeds of grain crops provides operations of sifting, drying, storage, division into fractions, pickling, packing in bags. A 5-zone grain dryer with step drying modes using a heat pump and a generator motor has been developed for seed grain. Comparison of energy efficiency of different drying units showed that the proposed technical solutions are verified by calculations and heat consumption is much lower and is 3024 kJ / kg vip . moisture.


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