scholarly journals 619 PB 325 Respiration as an indicator of health in excised, dormant vegetative apple buds subjected to cryopreservation treatments

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 521a-521
Author(s):  
L.E. Towill ◽  
S.A. Blackman

cryopreservation of dormant, vegetative apple buds at the National Seed Storage Laboratory is used to maintain a base collection for germplasm held in the National Clonal Germplasm Repository for apple and grape, Geneva, NY, and is performed by a method previously reported1. Growth of buds after grafting is now used to test for survival after exposure to and storage at very low temperatures (ca.-160°C). We are interested in determining if measures of respiration can be used to assess 1. the status of buds and bark used for preservation, 2. survival after different treatments related to cryopreservation, and 3. the extent of sublethal injury after treatments. A Licor 6252 CO2 analyzer was used to measure respiration. Reproducible measurements of respiration required at least 2-3 buds. Buds from winter harvested twigs (ca 45-48% moisture content) that were briefly warmed to room temperature respired at a rate of 34 umoles CO2 g-1 (dw) hr-1. Survival of buds is enhanced if twigs are dried prior to cooling. We found such treatments reduced respiration over non-dried controls. Respiration increased as the bud was rehydrated. Buds from dried twigs slowly cooled to low temperatures had levels of respiration after warming and rehydrating similar to undried, unfrozen controls. Buds from undried twigs directly placed at -196°C and warmed gave little CO2 production.

2019 ◽  
Vol 4 (1) ◽  
Author(s):  
Melati Melati ◽  
Devi Rusmin

One problem for developing small white ginger (Zingiber officinale var. amarum) is theavailability of high quality rhizome seeds in right quantity and time. Seed rhizome will sproutquickly, and its quality will decreased in immature seed rhizome and in not good storage conditions.The main objective of the experiment was to study the best storage room conditions for storagerhizome seeds of small white ginger. The experiment was conducted in seed laboratory and storageroom of Research Institute for Medicinal and Aromatic Crops Bogor. The experiment wasconducted by randomized complete design with nine different storage, 3 replication and 25 sampleseach treatment. The treatments are 1) rhizome seeds storage in room temperature (control),(2)rhizome seeds storage in AC room (16–240C),(3) rhizome seed soaked in paclobutrazol 1000 ppmfor 4 hours then seeds storage in room temperature ( 4) put rhizome seeds in rack and cover bystraw, storage in room temperature (5) rhizome seed storage on straws in the greenhouse ( 6)rhizome seed storage in the greenhouse without straw ( 7) rhizome seed soaked in water during 1hour every month then dried naturally in 300C and storage in AC room (8) rhizome seed soakedin paclobutrazol 1000 ppm for 4 hour then storage in AC room ( 9) put rhizome in wood box andcover by rice straw and husk. Variables observed include moisture contents of ginger seed, lostweight of seed and germination percentage of rhizome seeds at the end of storage period. The resultof experiment indicated that the moisture content and weight rhizome seeds decrease after 2 months. After four months storage period, moisture content was still high above 80 %, exceptrhizome seeds in under ground. The rhizome seeds that store in AC room showed the bestperformance and thus, this treatment can be recommended for storage of small white gingerrhizome seeds for 4 months. The low moisture content (< 80%) of rhizome small white ginger seedswill decreased its viability.Keywords: Zingiber officinale var. amarum, seed, storage, viability


Author(s):  
O. A. Zadorozhna ◽  
T. P. Shyianova ◽  
M.Yu. Skorokhodov

Seed longevity of 76 spring barley gene pool samples (Hordeum vulgare L. subsp. distichon, convar. distichon: 56 nutans Schubl., two deficience (Steud.) Koern., two erectum Rode ex Shuebl., two medicum Koern.; convar. nudum (L.) A.Trof.: one nudum L. та subsp. vulgare: convar. vulgare: nine pallidum Ser., three rikotense Regel.; convar. coeleste (L.) A.Trof.: one coeleste (L.) A.Trof.) from 26 countries, 11 years and four places of reproduction was analyzed. Seeds with 5–8% moisture content were stored in chamber with unregulated and 4oC temperature. The possibility of seed storage under these conditions for at least 10 years without significant changes in germination has been established. The importance of meteorological conditions in the formation and ripening of seeds for their longevity is confirmed. The relationship between the decrease of barley seeds longevity and storage conditions, amount of rainfall, temperature regime during the growing season of plants is discussed.


2021 ◽  
Vol 38 (1) ◽  
pp. 144-157
Author(s):  
Thaísa De Oliveira Silveira ◽  
Madelon Rodrigues Braz ◽  
Gilmara Pires de Moura Palermo ◽  
Tiago Böer Breier

Seeds of native forest species for food and seedling production have a growing demand that impacts the need for studies involving seed storage in order to maintain good levels of germination quality. Thus, scientific analyzes that explore the physiological potential of the seeds of these species are necessary, especially for the pink pepper (Schinus terebinthifolia Raddi.), a species used as an alternative source of income for traditional communities and which has been arousing interest due to the consumption of its fruits as a condiment in the national and international market. This work aimed to assess the physiological quality of pink pepper seeds according to the storage period and seed size. The seeds came from a rural settlement, a pioneer in the extraction of pink pepper, in the state of Rio de Janeiro, Brazil. The seeds were evaluated for moisture content, germination, first count, length and dry weight of the germinated seedlings, during the period of time that they remained stored and the size of the seeds. The results allowed to conclude that: i) the germinative percentage of the stored pink pepper seeds decreased over the months; ii) the moisture content of the seeds decreased over the months evaluated iii) the different sizes of seeds did not show differences in germination and storage time and iv) values of length and dry weight of the seedlings did not differ according to the period of storage.


2013 ◽  
Vol 23 (4) ◽  
pp. 257-269 ◽  
Author(s):  
K.M.G. Gehan Jayasuriya ◽  
Asanga S.T.B. Wijetunga ◽  
Jerry M. Baskin ◽  
Carol C. Baskin

AbstractSeeds of legumes are generally considered to have physical dormancy and to be orthodox, but most seed biologists are unaware of the various kinds and combinations of dormancy and storage behaviour in seeds of this family. The aim of our study was to document the dormancy and storage behaviour of seeds of 100 native and introduced tropical Fabaceae species in Sri Lanka and classify them into germination/storage behaviour categories. Moisture content (MC) was < 16% for fresh seeds of 94 species and >29% for those of six. Seeds of these six species had low tolerance for desiccation and for low temperatures. Thus, seeds of six species are non-orthodox and 94 species orthodox. Nine of the 100 species were non-dormant, and 2, 3 and 86 had physiological, physiological epicotyl and physical dormancy, respectively. Six germination/storage behaviour categories were identified among the 100 species. However, as in extratropical regions of the world, orthodox storage behaviour and physical dormancy are characteristic of seeds of the majority of species of Fabaceae in tropical Sri Lanka.


2019 ◽  
Vol 2 (3) ◽  
pp. 43
Author(s):  
. Sulassih ◽  
Yohanes Aris Purwanto ◽  
. Sobir ◽  
Naekman Naibaho ◽  
Siaga Yudha Pratama ◽  
...  

Viability of garlic seed is important for seed quality. Periode of seed storage Penyimpanan benih berkaitan dengan viabilitas benih. Viability testing measured after storage at 0 oC, 7 oC and room temperate. Moisture content decreased (40-45%) after 1 month in the storage under light condition, but the weight loss approximately at 1-8%, still lower than ASEAN rules (2009) that is not over than 10-15% for physical damage. Tawang Mangu variety showed higher for percentage of field emergence (83.33%) after was storages at 0 oC for one month and produced higher bulb (11 bulbs), but at the room temperature showed the lower for percentage of field emergence (56.94%) and produced 2-3 bulbs. All the treatment (7oC and room temperature) produced small size of bulbs in weight (1-2 g), height (1.5-1.7 cm) and width (1.5-1.6 cm). Three of the temperature condition was produced single bulb called “bawang lanang”, it suggested causing the land unfertilizer. Garlic was not suitable for medium land and high temperature such as Bogor.Keywords: diameter, height, male garlic, small, weight


AGROFOR ◽  
2018 ◽  
Vol 3 (2) ◽  
Author(s):  
Branko POPOVIĆ ◽  
Vele TEŠEVIĆ ◽  
Olga MITROVIĆ ◽  
Miodrag KANDIĆ ◽  
Aleksandar LEPOSAVIĆ ◽  
...  

Consumer acceptance of plum is based, primarily, on the corresponding sensorycharacteristics of the fruits: appearance, colour, firmness, taste and aroma. If notconsumed immediately after harvesting, the aforementioned characteristics of plumwill change. Still, those changes are less expressed when fruits are kept in coldstorage, at low temperatures. However, during the transport and storage of plum atroom temperature, which is often the case in local market plum sale, intensechanges of certain characteristics of the fruit happens as well as a significantshortening of shelf life. The most expressive is firmness change where fruitsbecome completely soft after seven days. Appearance and colour of the fruit areless susceptible to change. Since plum taste and odour depend on sugar and acidcontent, i.e., sugar-acid ratio and the content of certain volatile aromaticcomponents, changes of their contents have been examined in some importantplum cultivars in Serbia after seven-day storage at the temperature 20±1 °C. Sugaracidratio that determines sweetness, i.e., fruit acidity, has not significantly changedduring thе storage period. Among 10 examined aromatic components, the mostsignificant changes have been observed in 2-E-hexenal, which gives a distinctivegreen note aroma and nonanal, characteristic plum-like scent. During the seven-daystorage of picked plums, the content of 2-E-hexenal decreased while the content ofnonanal increased.


2020 ◽  
Author(s):  
Peter Murithi Angaine ◽  
Stephen Muriithi Ndungú ◽  
Alice Adongo Onyango ◽  
Jesse Omondi Owino

Abstract Background: Globally, forestry faces challenges in the availability of seeds due to limited knowledge on seed handling of various species. Forestry seeds are constantly being reviewed and classified as either recalcitrant, intermediate, or orthodox based on their storage behavior. It is essential to understand the tree seed storage behavior to maintain seed viability and thus minimize seed losses. There is scanty literature combining factors of seed moisture content (6%, 9%, 12%, 15%, and 20%), seed storage temperature (20oC, 5oC and -20oC), seed storage duration (1, 4, 9 and 12 months), and germination in different sites with varying environmental variables. Ehretia cymosa is important in the Afromontane forestry landscape as a medicinal, rehabilitation, and conservation species. This study conducted desiccation and storage studies and their influence on the viability of E. cymosa seeds. The study sought to determine the optimum conditions for the storage of Ehretia cymosa that maintains viability. Results: This study observed that E. cymosa dried to seed moisture content of 6%, stored for 12 months at 20oC and sown in the laboratory had the highest germination performance (27.6 ± 3.18%) (p<0.05). Conclusion: This confirms that E.cymosa seeds exhibit orthodox storage behavior. The authors recommend longer storage studies (>12months) to determine the actual longevity of the seeds of this species. The significance of these results would be useful for foresters and farmers that would need to use this species for various purposes.


1970 ◽  
Vol 6 (1) ◽  
pp. 24-33
Author(s):  
Ulfa Ridha Andini ◽  
Hot Setiado ◽  
Luthfi Aziz Mahmud Siregar

Amarilis is one of the flower of the park that is attractive and has high economic value. This study aims to determine the effect of vernalization on the appearance of local amaryllis plants in Tanah Karo and West Sumatra. This research was conducted at the Plant Breeding Basic Laboratory of the Faculty of Agriculture, University of North Sumatra, Medan, from February 2018 to July 2018. This research was carried out by storing tubers at room temperature (control) and storage of tubers at low temperatures (vernalization) at 10 oC for 6 weeks, and with differences in accession of plants namely G1 (Accession of West Sumatra Payakumbuh), G2 (Bukit Tinggi West Sumatra Accession), G3 (Padang Panjang West Sumatra Accession), G4 (Local Kabanjahe Accession Tanah Karo), G5 (Berastagi Local Accession Tanah Karo ), G6 (Tanah Karo Local Accession Accession). The results showed that vernalization gave a different appearance compared to the control treatment.


2019 ◽  
Vol 12 (1) ◽  
pp. 46 ◽  
Author(s):  
Marek Domin ◽  
Franciszek Kluza ◽  
Dariusz Góral ◽  
Sybilla Nazarewicz ◽  
Katarzyna Kozłowicz ◽  
...  

The present research attempts to characterize the effect of low temperatures, and the moisture content of maize (Kosmo 230) meant for sowing on its energy and capacity to germinate. Seeds were moistened to varying degrees and stored under various conditions; then, their germination energy and capacity were assessed. Sowing material with 15% moisture content showed slightly declined germination ability when stored at −25–−20 °C for over three days, while the storage of seeds with a 25 and 30% moisture content at −5–0 °C for 1–3 days had the effect of seed conditioning. Seedlings obtained from conditioned seeds showed sustainability characterized by faster growth, and demonstrated nearly twice the size as other plants. Warehousing and storage of maize grains with a 11.12% moisture content at temperatures up to −25 °C did not significantly affect seed germination capacity or energy, irrespective of storage time.


2012 ◽  
Vol 2 (2) ◽  
pp. 71-76
Author(s):  
Yantri Nuryani ◽  
Laili Susanti ◽  
Kurnia Herlina Dewi

Lemea is one of the unique food of Rejangnese which  made from bamboo shoots  and fermented fish.  This research is  aimed to find the effect of processing and storage temperature on moisture content, pH, total microbial and consumers preferences ( color, aroma, and taste) of sambal lemea. This research carried out by using CRD with two factors namely processing (pots and bamboo) and storage temperature (room temperature and refrigerator). The results showed that the treatment gives significant effect on moisture content, pH and the level of preference (color, aroma and flavor). While the temperature did not show significant differences.  Sambal lemea which cooked in bamboo has the highest preferences score on color, aroma and flavor on days 0 and 7.


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