Growth modelling of Listeria monocytogenes and Yersinia enterocolitica in food model systems and dairy products

1994 ◽  
Vol 24 (1-2) ◽  
pp. 83-92 ◽  
Author(s):  
M.Elisabetta Guerzoni ◽  
Rosalba Lanciotti ◽  
Sandra Torriani ◽  
Franco Dellaglio
Keyword(s):  
Listeria Monocytogenes ◽  
Yersinia Enterocolitica ◽  
Dairy Products ◽  
Model Systems ◽  
Growth Modelling ◽  
Food Model

A Validated PCR-Based Method To Detect Listeria monocytogenes Using Raw Milk as a Food Model—Towards an International Standard

2004 ◽  
Vol 67 (8) ◽  
pp. 1646-1655 ◽  
Author(s):  
M. D'AGOSTINO ◽  
M. WAGNER ◽  
J. A. VAZQUEZ-BOLAND ◽  
T. KUCHTA ◽  
R. KARPISKOVA ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Detection Probability ◽  
Dairy Products ◽  
Raw Milk ◽  
Correct Identification ◽  
Positive Signal ◽  
Pcr Assay ◽  
Collaborative Trial ◽  
Milk Samples ◽  
Food Model

A PCR assay with an internal amplification control was developed for Listeria monocytogenes. The assay has a 99% detection probability of seven cells per reaction. When tested against 38 L. monocytogenes strains and 52 nontarget strains, the PCR assay was 100% inclusive (positive signal from target) and 100% exclusive (no positive signal from nontarget). The assay was then evaluated in a collaborative trial involving 12 European laboratories, where it was tested against an additional 14 target and 14 nontarget strains. In that trial, the inclusivity was 100% and the exclusivity was 99.4%, and both the accordance (repeatability) and the concordance (reproducibility) were 99.4%. The assay was incorporated within a method for the detection of L. monocytogenes in raw milk, which involves 24 h of enrichment in half-Fraser broth followed by 16 h of enrichment in a medium that can be added directly into the PCR. The performance characteristics of the PCR-based method were evaluated in a collaborative trial involving 13 European laboratories. In that trial, a specificity value (percentage of correct identification of blank samples) of 81.8% was obtained; the accordance was 87.9%, and the concordance was 68.1%. The sensitivity (correct identification of milk samples inoculated with 20 to 200 L. monocytogenes cells per 25 ml) was 89.4%, the accordance was 81.2%, and the concordance was 80.7%. This method provides a basis for the application of routine PCR-based analysis to dairy products and other foodstuffs and should be appropriate for international standardization.

scholarly journals Natural Plant-Derived Chemical Compounds as Listeria monocytogenes Inhibitors In Vitro and in Food Model Systems

Pathogens ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 12
Author(s):  
Iwona Kawacka ◽  
Agnieszka Olejnik-Schmidt ◽  
Marcin Schmidt ◽  
Anna Sip
Keyword(s):  
Listeria Monocytogenes ◽  
Foodborne Pathogen ◽  
Chemical Compounds ◽  
Food Product ◽  
Model Systems ◽  
Biofilm Inhibition ◽  
Natural Plant ◽  
Alternative Delivery ◽  
Food Model

Listeria monocytogenes is a foodborne pathogen, sporadically present in various food product groups. An illness caused by the pathogen, named listeriosis, has high fatality rates. Even though L. monocytogenes is resistant to many environmental factors, e.g., low temperatures, low pH and high salinity, it is susceptible to various natural plant-derived antimicrobials (NPDA), including thymol, carvacrol, eugenol, trans-cinnamaldehyde, carvone S, linalool, citral, (E)-2-hexenal and many others. This review focuses on identifying NPDAs active against L. monocytogenes and their mechanisms of action against the pathogen, as well as on studies that showed antimicrobial action of the compounds against the pathogen in food model systems. Synergistic action of NDPA with other factors, biofilm inhibition and alternative delivery systems (encapsulation and active films) of the compounds tested against L. monocytogenes are also summarized briefly.

Effect of Ozone on the Inactivation of Yersinia enterocolitica and the Reduction of Natural Flora on Potatoes

2006 ◽  
Vol 69 (10) ◽  
pp. 2357-2363 ◽  
Author(s):  
MARÍA VICTORIA SELMA ◽  
DAVID BELTRÁN ◽  
ELISEO CHACÓN-VERA ◽  
MARÍA ISABEL GIL
Keyword(s):  
Listeria Monocytogenes ◽  
Yersinia Enterocolitica ◽  
Disease Outbreaks ◽  
Wash Water ◽  
Foodborne Disease ◽  
Pathogenic Microorganisms ◽  
Psychrotrophic Bacteria ◽  
Fresh Vegetables ◽  
Natural Flora ◽  
Soil Irrigation

Fresh vegetables contaminated with Yersinia enterocolitica have been implicated in foodborne disease outbreaks. Surfaces of vegetables can become contaminated with pathogenic microorganisms through contact with soil, irrigation water, fertilizers, equipment, humans, and animals. One approach to reduce this contamination is to treat fresh produce with sanitizers. In this study, the ability of ozone to inactivate Y. enterocolitica inoculated in water and on potato surfaces was evaluated. Furthermore, the efficacy of ozone in reducing natural flora on whole potato was determined. Total aerobic mesophilic and psychrotrophic bacteria, total coliforms, and Listeria monocytogenes were enumerated. Finally, several disinfection kinetic models were considered to predict Y. enterocolitica inactivation with ozone. Treatments with ozone (1.4 and 1.9 ppm) for 1 min decreased the Y. enterocolitica population in water by 4.6 and 6.2 log CFU ml−1, respectively. Furthermore, ozonated water (5 ppm) for 1 min decreased Y. enterocolitica and L. monocytogenes from potato surfaces by 1.6 and 0.8 log CFU g−1, respectively. Therefore, ozone can be an effective treatment for disinfection of wash water and for reduction of potato surface contamination.

DIAGNOSTIC PREPARATIONS FOR DETERMINING THE SPECIES OF THE BACTERIA LISTERIA MONOCYTOGENES

2021 ◽  
Author(s):  
V. G. Lobanova ◽  
Keyword(s):  
Listeria Monocytogenes ◽  
Dairy Products ◽  
Pathogenic Bacteria ◽  
Food Products ◽  
Nutrient Media ◽  
Microbiological Study ◽  
Highly Sensitive

This article presents a work on the microbiological study of food products for the presence of pathogenic bacteria Listeria monocytogenes, which can cause listeriosis in humans if sanitary and hygienic rules and regulations are not followed. Highly sensitive and specific nutrient media make it possible to quickly and efficiently test meat, vegetable and dairy products for the detection of Listeria monocytogenes.

Occurrence of Listeria monocytogenes in Milk and Dairy Products Produced or Imported Into Morocco

1993 ◽  
Vol 56 (3) ◽  
pp. 256-259 ◽  
Author(s):  
A. EL MARRAKCHI ◽  
A. HAMAMA ◽  
F. EL OTHMANI
Keyword(s):  
Listeria Monocytogenes ◽  
Dairy Products ◽  
Raw Milk ◽  
Listeria Innocua ◽  
Pasteurized Milk ◽  
Listeria Species ◽  
Fresh Cheese ◽  
Milk And Dairy Products

Examination of 227 samples of milk and dairy products for Listeria monocytogenes showed that raw milk and some Moroccan traditionally made dairy products such as Iben and raib (fermented milks) and jben (fresh cheese) were contaminated with this pathogen. L. monocytogenes was the only Listeria species isolated except in one case in which it was associated with Listeria innocua. Pasteurized milk, fresh cream, and fresh and ripened cheeses (industrially made) were free from L. monocytogenes.

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