Evaluation of enrichment procedures for recovering Listeria monocytogenes from dairy products

1989 ◽  
Vol 9 (3) ◽  
pp. 249-268 ◽  
Author(s):  
A Lammerding
Keyword(s):  
Listeria Monocytogenes ◽  
Dairy Products

DIAGNOSTIC PREPARATIONS FOR DETERMINING THE SPECIES OF THE BACTERIA LISTERIA MONOCYTOGENES

2021 ◽  
Author(s):  
V. G. Lobanova ◽  
Keyword(s):  
Listeria Monocytogenes ◽  
Dairy Products ◽  
Pathogenic Bacteria ◽  
Food Products ◽  
Nutrient Media ◽  
Microbiological Study ◽  
Highly Sensitive

This article presents a work on the microbiological study of food products for the presence of pathogenic bacteria Listeria monocytogenes, which can cause listeriosis in humans if sanitary and hygienic rules and regulations are not followed. Highly sensitive and specific nutrient media make it possible to quickly and efficiently test meat, vegetable and dairy products for the detection of Listeria monocytogenes.

Occurrence of Listeria monocytogenes in Milk and Dairy Products Produced or Imported Into Morocco

1993 ◽  
Vol 56 (3) ◽  
pp. 256-259 ◽  
Author(s):  
A. EL MARRAKCHI ◽  
A. HAMAMA ◽  
F. EL OTHMANI
Keyword(s):  
Listeria Monocytogenes ◽  
Dairy Products ◽  
Raw Milk ◽  
Listeria Innocua ◽  
Pasteurized Milk ◽  
Listeria Species ◽  
Fresh Cheese ◽  
Milk And Dairy Products

Examination of 227 samples of milk and dairy products for Listeria monocytogenes showed that raw milk and some Moroccan traditionally made dairy products such as Iben and raib (fermented milks) and jben (fresh cheese) were contaminated with this pathogen. L. monocytogenes was the only Listeria species isolated except in one case in which it was associated with Listeria innocua. Pasteurized milk, fresh cream, and fresh and ripened cheeses (industrially made) were free from L. monocytogenes.

Identification and Enumeration of Beta-Hemolytic Listeria monocytogenes in Naturally Contaminated Dairy Products

1988 ◽  
Vol 71 (3) ◽  
pp. 673-675
Author(s):  
Atin R Datta ◽  
Barry A Wentz ◽  
Walter E Hill
Keyword(s):  
Listeria Monocytogenes ◽  
Dairy Products ◽  
Dna Probe ◽  
Milk Samples ◽  
Unpasteurized Milk ◽  
Pure Cultures ◽  
Camp Test

Abstract A DNA probe was used to identify hemolytic Listeria monocytogenes in naturally contaminated dairy products: unpasteurized milk, ricotta cheese, and imported semisoft cheeses. Of 34 milk samples, 12 were suspected to contain hemolytic L. monocytogenes; 1 contained >6000 viable organisms/g. The ricotta cheese, although temperature-abused, had a titer of 3.6 x 10-6 beta-hemolytic L. monocytogenes cells/g, whereas the semisoft cheeses reached a maximum of 5.6 x 10-6 cells/g. Pure cultures of L. monocytogenes isolated from both types of cheese were found positive by the CAMP test and the DNA probe

scholarly journals Survivability of Salmonella and Escherichia coli O157:H7 Pathogens and Food Safety Concerns on Commercial Powder Milk Products

Dairy ◽  
2020 ◽  
Vol 1 (3) ◽  
pp. 189-201 ◽  
Author(s):  
Roshan Paswan ◽  
Young W. Park
Keyword(s):  
Escherichia Coli ◽  
Listeria Monocytogenes ◽  
Dairy Products ◽  
Milk Powder ◽  
Escherichia Coli O157 ◽  
Foodborne Illnesses ◽  
Milk Products ◽  
Salmonella Infections ◽  
Powder Milk ◽  
The U.S

Milk and dairy products are susceptible to the incidence of foodborne illnesses by numerous pathogens, including Listeria monocytogenes, Salmonella spp., Escherichia coli, enteropathogenic Campylobacter jejuni, Yersinia enterocolitica, Cronobacter (Enterobacter sakazakii) and Staphylococcus aureus. Annually Salmonella infections cause approximately 93.8 million cases of gastroenteritis and 155,000 deaths worldwide. Including meat and poultry, dairy products are the most commonly contaminated foods by Salmonella. Studies show that Salmonella, Escherichia coli O157:H7 and Listeria monocytogenes are among the top 5 pathogens causing hospitalization and life-threatening foodborne illnesses. The U.S. Centers for Disease Control and Prevention (CDC) estimated that annually around 1.2 million foodborne illnesses with more than 23,000 hospitalizations, 450 deaths and 130 outbreaks were attributed to Salmonella infection in the U.S. The Salmonella enteric in skim milk powder survived at three months storage, with water activity as low as 0.33. With respect to Escherichia coli O157:H7, it is capable of causing disease at a low dosage, ranging from 5–50 cells. Viable cells of Escherichia coli O157:H7 reportedly survive in infant formula powder for one year at 5 °C. The survivability of Escherichia coli in powder milk was significantly reduced with the synergistic effects of storage time and temperature. The U.S. Dairy Export Council recommends that milk powder should be stored in a cool and dry place, at a temperature not to exceed 27 °C, and a relative humidity not to exceed 65%. Reports have recommended that milk powder products need to be stored in light, oxygen, and moisture-proof containers. In this article, the survival of the major foodborne pathogens including Salmonella and Escherichia coli O157:H7 in powdered milk products from common dairy species such as cow and goats are reviewed.

Comparison of EB and Fraser Enrichment Broths for the Detection of Listeria spp. and Listeria monocytogenes in Raw-Milk Dairy Products and Environmental Samples

1996 ◽  
Vol 59 (11) ◽  
pp. 1172-1175 ◽  
Author(s):  
GEERTRUI M. VLAEMYNCK ◽  
RENAAT MOERMANS
Keyword(s):  
Listeria Monocytogenes ◽  
Dairy Products ◽  
Raw Milk ◽  
Chain Reaction ◽  
Enrichment Broth ◽  
Selective Enrichment ◽  
Isolation Frequency ◽  
Detection And Identification ◽  
Significant Difference ◽  
Polymerase Chain

This study is a comparison of the isolation frequency of Listeria spp. and Listeria monocytogenes from selected naturally contaminated dairy products, especially soft smear-ripened cheeses from raw milk and samples of feces and rinse samples from the udder taken on the farm, by using an enrichment broth (EB) recommended by the International Dairy Federation and the U.S. Food and Drug Administration (IDF and FDA) or Fraser broth as the selective enrichment. Detection and identification were carried out according to the IDF protocols and a polymerase chain reaction technique. Listeria spp. were detected in 39.8% of the 570 samples while 15.3% were positive for L. monocytogenes. For cheese and curd samples, Fraser enrichment broth gave a statistically significant higher recovery for all Listeria spp. (26 to 21 %) as well as for L. monocytogenes in particular (9 to 1.4%). For raw milk and samples taken from feces and the udder rinse no significant difference was found between EB and Fraser broth. A combination of both enrichments resulted in an increase of recovery over all matrices by 15%.

scholarly journals Collection of Listeria monocytogenes Isolates from Milk, Dairy Products and Food Processing Environments in Slovakia for the Purposes of European Molecular Database

2017 ◽  
Vol 61 (1) ◽  
pp. 60-65
Author(s):  
Z. Kubicová ◽  
M. Filipová ◽  
J. Jurovčíková ◽  
L Cabanová
Keyword(s):  
Listeria Monocytogenes ◽  
Dairy Products ◽  
Food Chains ◽  
Reference Laboratory ◽  
The European Union ◽  
Comparison Analysis ◽  
European Union Reference Laboratory ◽  
And Control ◽  
Different Sources ◽  
Epidemiological Information

Abstract The molecular typing of Listeria monocytogenes isolates is an important tool for monitoring the spread of the strains in food chains, providing evidence for epidemiological investigations and for the detection of out-breaks. The demand of European typing data centralization, collection and sharing stimulated the generation of “EURL L. monocytogenes Database (EURL Lm DB)” in 2012 led by the European Union Reference Laboratory (EURL) for L. monocytogenes (ANSES Maisons-Alfort Laboratory for Food Safety, France) in close collaboration with Applied Maths. This database includes the typing results and epidemiological information on strains isolated from food, environmental or animal samples and it is in connection with human strains database TESSy (The European Surveillance System) led by the ECDC (European Centre for Disease Prevention and Control). In total 147 L. monocytogenes isolates were examined by PFGE (pulsed field gel electrophoresis) in 2014—2015 in VFI Dolny Kubin from different sources. Nearly half (68) of the 147 isolates in the national Slovak database came from milk or dairy products samples and the related manufacturing environment. In this work, 68 isolates associated with milk were selected and divided into 27 clusters (95 % similarity level) after combined comparison analysis (AscI and ApaI) by BioNumerics 6.6 software. Eight clusters included three or more similar PFGE profiles.

scholarly journals Population Genetic Structure of Listeria monocytogenes Strains as Determined by Pulsed-Field Gel Electrophoresis and Multilocus Sequence Typing

2016 ◽  
Vol 82 (18) ◽  
pp. 5720-5728 ◽  
Author(s):  
Clémentine Henri ◽  
Benjamin Félix ◽  
Laurent Guillier ◽  
Pimlapas Leekitcharoenphon ◽  
Damien Michelon ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Food Safety ◽  
Genetic Structure ◽  
Gel Electrophoresis ◽  
Dairy Products ◽  
Population Genetic ◽  
Meat Products ◽  
Pulsed Field Gel Electrophoresis ◽  
Content Type ◽  
Food Matrices

ABSTRACTListeria monocytogenesis a ubiquitous bacterium that may cause the foodborne illness listeriosis. Only a small amount of data about the population genetic structure of strains isolated from food is available. This study aimed to provide an accurate view of theL. monocytogenesfood strain population in France. From 1999 to 2014, 1,894L. monocytogenesstrains were isolated from food at the French National Reference Laboratory forL. monocytogenesand classified according to the five risk food matrices defined by the European Food Safety Authority (EFSA). A total of 396 strains were selected on the basis of different pulsed-field gel electrophoresis (PFGE) clusters, serotypes, and strain origins and typed by multilocus sequence typing (MLST), and the MLST results were supplemented with MLST data available from Institut Pasteur, representing human and additional food strains from France. The distribution of sequence types (STs) was compared between food and clinical strains on a panel of 675 strains. High congruence between PFGE and MLST was found. Out of 73 PFGE clusters, the two most prevalent corresponded to ST9 and ST121. Using original statistical analysis, we demonstrated that (i) there was not a clear association between ST9 and ST121 and the food matrices, (ii) serotype IIc, ST8, and ST4 were associated with meat products, and (iii) ST13 was associated with dairy products. Of the two major STs, ST121 was the ST that included the fewest clinical strains, which might indicate lower virulence. This observation may be directly relevant for refining risk analysis models for the better management of food safety.IMPORTANCEThis study showed a very useful backward compatibility between PFGE and MLST for surveillance. The results enabled better understanding of the population structure ofL. monocytogenesstrains isolated from food and management of the health risks associated withL. monocytogenesfood strains. Moreover, this work provided an accurate view ofL. monocytogenesstrain populations associated with specific food matrices. We clearly showed that some STs were associated with food matrices, such as meat, meat products, and dairy products. We opened the way to source attribution modeling in order to quantify the relative importance of the main food matrices.

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