Differentiation of isolated calvarial cells into a mature heterogeneous bone cell population in culture

1978 ◽  
Vol 1 (1) ◽  
pp. 75-79 ◽  
Author(s):  
Arnold J. Altman ◽  
Janet G. Bandelin ◽  
Jesus H. Dominguez ◽  
Gregory R. Mundy
Keyword(s):  
2021 ◽  
pp. 33-52
Author(s):  
Mahziyar Darvishi ◽  
Hooman Dadras ◽  
Mohammad Mahmoodi Gahrouei ◽  
Kiarash Tabesh ◽  
Dmitry Timofeev

Author(s):  
Joana Reis ◽  
António Ramos

Oxidative stress plays a central role in physiological and pathological bone conditions. Its role in signalment and control of bone cell population differentiation, activity, and fate is increasingly recognized. The possibilities of its use and manipulation with therapeutic goals are virtually unending. However, how redox balance interplays with the response to mechanical stimuli is yet to be fully understood. The present work summarizes current knowledge on these aspects, in an integrative and broad introductory perspective.


Author(s):  
J. Russo ◽  
W. Isenberg ◽  
M. Ireland ◽  
I.H. Russo

The induction of rat mammary carcinoma by the chemical carcinogen DMBA is used as a model for the study of the human disease (1). We previously described the histochemical changes that occur in the mammary gland of DMBA treated animals before the earliest manifested histological change, the intraductal proliferation (IDP), was observed (2). In the present work, we demonstrate that a change in the stable cell population found in the resting mammary gland occurs after carcinogen administration.Fifty-five day old Sprague-Dawley virgin female rats were inoculated intragastrically with 20mg of 7,12-dimethylbenz(a)anthracene (DMBA) in 1ml sesame oil. Non-inoculated, age-matched females were used as controls. Mammary glands from control and inoculated rats were removed weekly from the time of inoculation until 60 days post-inoculation. For electron microscopy, the glands were immersed in Karnovsky's fixative, post-fixed in 1% OsO4, dehydrated, and embedded in an Epon-Araldite mixture. Thick (lμ) sections were stained with 1% toluidine blue and were used for selecting areas for ultrastructural study.


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