Rat peritoneal macrophage procoagulant and fibrinolytic activities. An expression of the local inflammatory response

Endotoxins play a crucial role in ruminant health due to their deleterious effects on animal health. The study aimed to evaluate whether LPS and LTA can induce an inflammatory response in rumen epithelial cells. For this purpose, epithelial cells isolated from rumen tissue (RECs) were stimulated with LPS and LTA for 1, 2, 4, and 24 h. Thereafter, the expression of selected genes of the LPS and LTA pathway and inflammatory response were evaluated. Furthermore, it was assessed whether LPS affects inflammatory response and structural integrity of claw explants. Therefore, claw explants were incubated with LPS for 4 h to assess the expression of selected genes and for 24 h to evaluate tissue integrity via separation force. LPS strongly affected the expression of genes related to inflammation (NFkB, TNF-α, IL1B, IL6, CXCL8, MMP9) in RECs. LTA induced a delayed and weaker inflammatory response than LPS. In claw explants, LPS affected tissue integrity, as there was a concentration-dependent decrease of separation force. Incubation time had a strong effect on inflammatory genes in claw explants. Our data suggest that endotoxins can induce a local inflammatory response in the rumen epithelium. Furthermore, translocation of LPS might negatively impact claw health.

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Localized pancreatic NF-κB activation and inflammatory response in taurocholate-induced pancreatitis

AJP Gastrointestinal and Liver Physiology ◽

10.1152/ajpgi.2001.280.6.g1197 ◽

2001 ◽

Vol 280 (6) ◽

pp. G1197-G1208 ◽

Cited By ~ 104

Author(s):

Eva Vaquero ◽

Ilya Gukovsky ◽

Vjekoslav Zaninovic ◽

Anna S. Gukovskaya ◽

Stephen J. Pandol

Keyword(s):

Transcription Factor ◽

Inflammatory Response ◽

Pancreatic Head ◽

Pancreatic Injury ◽

Nuclear Factor Κb ◽

Saline Infusion ◽

Activator Protein 1 ◽

Local Inflammatory Response ◽

Monocyte Chemoattractant Protein 1 ◽

Factor Α

Transcription factor nuclear factor-κB (NF-κB) is activated in cerulein pancreatitis and mediates cytokine expression. The role of transcription factor activation in other models of pancreatitis has not been established. Here we report upregulation of NF-κB and inflammatory molecules, and their correlation with local pancreatic injury, in a model of severe pancreatitis. Rats received intraductal infusion of taurocholate or saline, and the pancreatic head and tail were analyzed separately. NF-κB and activator protein-1 (AP-1) activation were assessed by gel shift assay, and mRNA expression of interleukin-6, tumor necrosis factor-α, KC, monocyte chemoattractant protein-1, and inducible nitric oxide synthase was assessed by semiquantitative RT-PCR. Morphological damage and trypsin activation were much greater in the pancreatic head than tail, in parallel with a stronger activation of NF-κB and cytokine mRNA. Saline infusion mildly affected these parameters. AP-1 was strongly activated in both pancreatic segments after either taurocholate or saline infusion. NF-κB inhibition with N-acetylcysteine ameliorated the local inflammatory response. Correlation between localized NF-κB activation, cytokine upregulation, and tissue damage suggests a key role for NF-κB in the development of the inflammatory response of acute pancreatitis.

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Microarray analysis reveals a local inflammatory response induced by cardiopulmonary bypass in rat kidney

European Journal of Anaesthesiology ◽

10.1097/00003643-201106001-00208 ◽

2011 ◽

Vol 28 ◽

pp. 66

Author(s):

H. R. Bouma ◽

I. V. Samarska ◽

M. M.R.F. Struys ◽

R. H. Henning ◽

A. H. Epema

Keyword(s):

Cardiopulmonary Bypass ◽

Inflammatory Response ◽

Microarray Analysis ◽

Rat Kidney ◽

Local Inflammatory Response

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Priming of human neutrophils by peroxynitrite: potential role in enhancement of the local inflammatory response

Journal of Leukocyte Biology ◽

10.1002/jlb.65.1.59 ◽

1999 ◽

Vol 65 (1) ◽

pp. 59-70 ◽

Cited By ~ 36

Author(s):

Troy T. Rohn ◽

Laura K. Nelson ◽

Karen M. Sipes ◽

Steve D. Swain ◽

Kathryn L. Jutila ◽

...

Keyword(s):

Inflammatory Response ◽

Potential Role ◽

Human Neutrophils ◽

Local Inflammatory Response

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Diesel exhaust particles, the local inflammatory response, and the systemic IgE response to ovalbumin

Toxicology Letters ◽

10.1016/s0378-4274(96)80053-1 ◽

1996 ◽

Vol 88 ◽

pp. 15

Author(s):

Martinus Løvik ◽

Per Ivar Gaarder ◽

Ann-Kristin Høgseth ◽

Randi Hagemann ◽

Ingvar Eide

Keyword(s):

Inflammatory Response ◽

Diesel Exhaust ◽

Diesel Exhaust Particles ◽

Local Inflammatory Response ◽

Exhaust Particles ◽

Ige Response

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Different experimental multiple trauma models induce comparable inflammation and organ injury

Scientific Reports ◽

10.1038/s41598-020-76499-z ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Borna Relja ◽

Bing Yang ◽

Katrin Bundkirchen ◽

Baolin Xu ◽

Kernt Köhler ◽

...

Keyword(s):

Inflammatory Response ◽

Multiple Trauma ◽

Inflammatory Cells ◽

Organ Damage ◽

Organ Injury ◽

Local Inflammatory Response ◽

Healthy Control ◽

Post Traumatic ◽

Tissue Trauma ◽

Trauma Group

AbstractMultiple injuries appear to be a decisive factor for experimental polytrauma. Therefore, our aim was to compare the inflammatory response and organ damage of five different monotrauma with three multiple trauma models. For this, mice were randomly assigned to 10 groups: Healthy control (Ctrl), Sham, hemorrhagic shock (HS), thoracic trauma (TxT), osteotomy with external fixation (Fx), bilateral soft tissue trauma (bsTT) or laparotomy (Lap); polytrauma I (PT I, TxT + HS + Fx), PT II (TxT + HS + Fx + Lap) and one multi-trauma group (MT, TxT + HS + bsTT + Lap). The inflammatory response and organ damage were quantified at 6 h by analyses of IL-6, IL-1β, IL-10, CXCL1, SAA1, HMGB1 and organ injury. Systemic IL-6 increased in all mono and multiple trauma groups, while CXCL1 increased only in HS, PT I, PT II and MT vs. control. Local inflammatory response was most prominent in HS, PT I, PT II and MT in the liver. Infiltration of inflammatory cells into lung and liver was significant in all multiple trauma groups vs. controls. Hepatic and pulmonary injury was prominent in HS, PT I, PT II and MT groups. These experimental multiple trauma models closely mimic the early post-traumatic inflammatory response in human. Though, the choice of read-out parameters is very important for therapeutic immune modulatory approaches.

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The familial trend of the local inflammatory response in periodontal disease

Oral Diseases ◽

10.1111/odi.13738 ◽

2020 ◽

Author(s):

Mabelle Freitas Monteiro ◽

Márcio Zaffalon Casati ◽

Enilson Antonio Sallum ◽

Karina Gonzales Silvério ◽

Francisco Humberto Nociti‐Jr ◽

...

Keyword(s):

Inflammatory Response ◽

Periodontal Disease ◽

Local Inflammatory Response

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Preoperative irrigation and vacuum sealing drainage with antibiotic-containing drainage fluid of foot and ankle wounds improves outcome of reconstructive skin flap surgery

Journal of Orthopaedic Surgery and Research ◽

10.1186/s13018-019-1418-0 ◽

2019 ◽

Vol 14 (1) ◽

Author(s):

Xu Gao ◽

Hailei Yin ◽

Jixia Sun

Keyword(s):

Inflammatory Response ◽

Skin Flap ◽

Foot And Ankle ◽

Flap Surgery ◽

Local Inflammatory Response ◽

Drainage Fluid ◽

Before And After ◽

Group A ◽

Group B ◽

Vacuum Sealing Drainage

Abstract Objectives By observing the infection and soft tissue defect on the wound surface of the foot and ankle, this paper attempts to explore the effect of preoperative irrigation and vacuum sealing drainage with antibiotic-containing drainage fluid (abPI-VSD) on the bacterial quantity and the local inflammatory response at the flap, and further to provide a basis for applying this technique before a reconstructive skin flap surgery of foot and ankle wounds. Methods Seventy-five patients were randomly divided into two groups, and all surgeries were done by one physician. The flap reconstructions were done to 31 cases with the abPI-VSD being used (group A); the flap reconstructions were done to the rest 44 cases after wound cleaning using antibiotic irrigation solution without the use of the abPI-VSD (group B). Quantitative bacteriology was made to group A before and after the use of abPI-VSD; quantitative bacteriology was made to group B before and after wound cleaning. Then, the reconstructive skin flap surgery was done. After the surgeries, the time of local inflammatory response at the flap in both groups were recorded. The measured bacterial quantity was evaluated in logarithm and by t test. Results The bacterial quantity was 3.2 ± 1.9 × 107 cfu/g in group A before the use of abPI-VSD and 2.3 ± 2.0 × 107 in group B (P > 0.05) before debridement. The bacterial quantity was 1.2 ± 2.0 × 104 cfu/g in group A after abPI-VSD and was 2.9 ± 4.0 × 106 in group B after wound cleaning (P < 0.05). The time of postoperative inflammatory response in the flap was 8 ± 2.5 days in group A and 13 ± 3.4 days in group B (P < 0.05). Conclusions abPI-VSD can distinctly reduce the bacterial quantity on the surface of the wound, provide a good condition of tissue bed for the flap reconstruction, and effectively control the local inflammatory response at the flap and hence improve the survival quality of the flap.

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Inhibition of the Local Inflammatory Response in Man by Antimetabolites

Blood ◽

10.1182/blood.v27.1.38.38 ◽

1966 ◽

Vol 27 (1) ◽

pp. 38-48 ◽

Cited By ~ 70

Author(s):

EVAN M. HERSH ◽

VERNON G. WONG ◽

EMIL J. FREIREICH

Keyword(s):

Combination Therapy ◽

Inflammatory Response ◽

Acute Leukemia ◽

Mononuclear Cell ◽

Antitumor Agents ◽

Local Inflammatory Response ◽

Inflammatory Site ◽

The Individual

Abstract The effects of chemotherapy on the local inflammatory response in man were studied in 33 patients. Inhibition of the mononuclear cell phase of the response at 4, 8 and 24 hours was noted. The median on-therapy values at these hours were 0, 8.5 and 23 per cent compared to control values of 4, 24.5 and 86 per cent, respectively. The 24-hour values for the individual types of therapy were 13.5 per cent for intensive combination therapy with 4 antitumor agents, 21 per cent for 6-mercaptopurine given i.v. daily for 5 days, 2 per cent for methotrexate given i.v. daily for 5 days, 37 per cent for intermittent methotrexate and 56 per cent for other therapy. Recovery was substantial within 5 days of the end of therapy. The inhibition was not correlated with alterations in the levels of circulating granulocytes or lymphocytes, nor with the numbers of granulocytes entering the inflammatory site. Complicating infections were noted in eight patients, seven of whom were in the most strongly inhibited group. Observations of the response in ten untreated patients with acute leukemia showed both impaired granulocyte and mononuclear phases of the response.

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