Hemolysis and membrane lipid changes induced by xanthine oxidase in vitamin E deficient red cells

1986 ◽  
Vol 2 (1) ◽  
pp. 49-56
Author(s):  
H TAMAI ◽  
M MIKI ◽  
M MINO
Keyword(s):  
Vitamin E ◽  
Xanthine Oxidase ◽  
Membrane Lipid ◽  
Red Cells

scholarly journals Tocotrienol Rich Palm Oil Extract Is More Effective Than Pure Tocotrienols at Improving Endothelium-Dependent Relaxation in the Presence of Oxidative Stress

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Saher F. Ali ◽  
Owen L. Woodman
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
Endothelial Function ◽  
Xanthine Oxidase ◽  
Palm Oil ◽  
Oxidant Stress ◽  
Rat Aorta ◽  
Isolated Aorta ◽  
Oil Extract ◽  
Endothelium Dependent Relaxation

Oxidative endothelial dysfunction is a critical initiator of vascular disease. Vitamin E is an effective antioxidant but attempts to use it to treat vascular disorders have been disappointing. This study investigated whether tocotrienols, the less abundant components of vitamin E compared to tocopherols, might be more effective at preserving endothelial function. Superoxide generated by hypoxanthine/xanthine oxidase or rat aorta was measured using lucigenin-enhanced chemiluminescence. The effect ofα-tocopherol,α-,δ-, andγ-tocotrienols and a tocotrienol rich palm oil extract (tocomin) on levels of superoxide was assessed. Endothelial function in rat aorta was assessed in the presence of the auto-oxidant pyrogallol. Whilst all of the compounds displayed antioxidant activity, the tocotrienols were more effective when superoxide was produced by hypoxanthine/xanthine oxidase whereas tocomin andα-tocopherol were more effective in the isolated aorta. Tocomin andα-tocopherol restored endothelial function in the presence of oxidant stress butα-,δ-, andγ-tocotrienols were ineffective. The protective effect of tocomin was replicated when the tocotrienols were present with, but not without,α-tocopherol. Tocotrienol rich tocomin is more effective thanα-tocopherol at reducing oxidative stress and restoring endothelium-dependent relaxation in rat aortae and althoughα-,δ-, andγ-tocotrienols effectively scavenged superoxide, they did not improve endothelial function.

Vitamin E Deficiency and Xanthine Oxidase in Rabbits.

1953 ◽  
Vol 84 (2) ◽  
pp. 468-470 ◽  
Author(s):  
D. A. Richert ◽  
W. W. Westerfeld
Keyword(s):  
Vitamin E ◽  
Xanthine Oxidase ◽  
Vitamin E Deficiency

Changes in membrane constituents and chemiluminescence in vitamin E-deficient red blood cells induced by the xanthine oxidase reaction

1989 ◽  
Vol 272 (1) ◽  
pp. 81-87 ◽  
Author(s):  
Hiroshi Yasuda ◽  
Masayuki Miki ◽  
Yoshito Takenaka ◽  
Hiroshi Tamai ◽  
Makoto Mino
Keyword(s):  
Vitamin E ◽  
Red Blood Cells ◽  
Xanthine Oxidase ◽  
Blood Cells ◽  
Oxidase Reaction

Antioxidant supplementation of boar spermatozoa from different fractions of the ejaculate improves cryopreservation: changes in sperm membrane lipid architecture

Zygote ◽  
2004 ◽  
Vol 12 (2) ◽  
pp. 117-124 ◽  
Author(s):  
F.J. Peña ◽  
A. Johannisson ◽  
M. Wallgren ◽  
H. Rodriguez Martinez
Keyword(s):  
Vitamin E ◽  
Sperm Quality ◽  
Membrane Lipid ◽  
Water Soluble ◽  
Sample Design ◽  
Antioxidant Supplementation ◽  
Water Soluble Vitamin ◽  
Split Sample ◽  
The Status ◽  
Boar Spermatozoa

Previous studies have shown sperm quality after cryopreservation differs depending on the fraction of seminal plasma the boar spermatozoa are contained in. Thus, spermatozoa contained in the first 10 ml of the sperm-rich fraction (portion I) withstand handling procedures (extension, handling and freezing/thawing) better than those contained in the latter part of a fractionated ejaculate (second portion of the sperm-rich fraction and the post-spermatic fraction; portion II). The present study evaluated whether an exogenous antioxidant, the water-soluble vitamin E analogue Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid), could, when added to the freezing extender in a split-sample design trial, improve the post-thaw viability and membrane quality of this particular portion of the ejaculate, with particular attention to the status of the plasma membrane. Using a split-sample design, the initial changes in the fluidity status of the sperm plasmalemma after thawing were measured by flow cytometry (FC) after loading with Merocyanine-540 and YO-PRO-1. The FC-derived data revealed a clear ejaculate portion-dependent effect of the antioxidant supplementation. While no beneficial effect of the antioxidant supplementation was visible in spermatozoa from portion I, more spermatozoa with intact membranes were observed in the supplemented samples of portion II, suggesting the protective effect of vitamin E is dependent of the portion of the boar ejaculate considered.

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